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Tissue References Search Tool

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Adipose/Fat
Fish
Fish, Atlantic salmon
Todorcevic, M. , Vegusdal, A. , Gjoen, T. , Sundvold, H. , Torstensen, B. , Kjaer, M. and Ruyter, B.
Changes in Fatty Acids Metabolism During Differentiation of Atlantic Salmon Preadipocytes; Effects of n-3 and n-9 Fatty Acids. ,
Biochim Biophys Acta
 1781 , 326 , 2008
Cells
Preadipocytes
Enzymes
Medium
HBSS
Adipose/Fat
Hamster
Hamster, adult
(also rat, gerbil, rabbit, lamb, guinea-pig)
Nedergaard, J. and Lindberg, O.
The Brown Fat Cell ,
Int Rev Cytol
 74 , 187 , 1982
Cells
Brown fat
Enzymes
Medium
Bicarbonate buffer
Adipose/Fat
Hamster
Hamster, 5 week-12 month
DiGirolamo, M. , Mendlinger, S. and Fertig, J.W.
A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species ,
Am J Physiol
 221 , 850 , 1971
Cells
White fat
Enzymes
Collagenase : 0.05%
Medium
Kreb's Ringer bicarbonate buffer
Adrenal
Hamster
Hamster (Mesocricetus auratus) 100-150 g
Liang, B.T. and Perlman, R.L.
Catecholamine Secretion by Hamster Adrenal Cells ,
J Neurochem
 32 , 927 , 1979
Cells
Adrenal
Chromaffin
Enzymes
Hyaluronidase : 0.20%
Medium
Kreb's Ringer bicarbonate buffer
Adrenal
Ovine
Ovine, adult and fetal
Valego, N. and Rose, J.
A Specific CRH Antagonist Attenuates ACTH-Stimulated Cortisol Secretion in Ovine Adrenocortical Cells. ,
Reprod Sci
 17 , 477 , 2010
Cells
Adrenocortical
Enzymes
Medium
DMEM/Ham's F12
Adrenal
Ovine
Ovine, fetal
Valego, N. , Su, Y. , Carey, L. , Young, S. , Tatter, S. , Wang, J. and Rose, J.
Hypothalamic-Pituitary Disconnection in Fetal Sheep Blocks the Peripartum Increases in Adrenal Responsiveness and Adrenal ACTH Receptor Expression. ,
Am J Physiol Regul Integr Comp Physiol
 289 , R410 , 2005
Cells
Adrenocortical
Enzymes
Medium
DMEM/Ham's F12
Adrenal
Ovine
Ovine, adult
Keating, D. , Rychkov, G. , Adams, M. , Holgert, H. , McMillen, I.C. and Roberts, M.
Opioid Receptor Stimulation Suppresses the Adrenal Medulla Hypoxic Response in Sheep by Actions on Ca(2+) and K(+) Channels. ,
J Physiol
 555 , 489 , 2004
Cells
Chromaffin cells
Enzymes
Medium
Locke's solution
Adrenal
Ovine
Ovine, 3 year
Canny B J, O'Farrell K A, Clarke I J, Tilbrook A J.
The influence of sex and gonadectomy on the hypothalamo-pituitary-adrenal axis of the sheep ,
J Endocrinol
 162 , 215-25 , 1999
Cells
Anterior pituitary
Enzymes
Trypsin : 2.5%
Medium
DMEM
Brain
Ovine
Ovine
Reddy, R. , Amodei, R. , Estill, C. , Stormshak, F. , Meaker, M. and Roselli, C.
Effect of Testosterone on Neuronal Morphology and Neuritic Growth of Fetal Lamb Hypothalamus-Preoptic Area and Cerebral Cortex in Primary Culture. ,
PLoS ONE
 10 , e0129521 , 2015
Cells
Neurons
Enzymes
Papain : 0.2%
Medium
Hibernate
Brain
Ovine
Ovine
Lepore G, Gadau S, Mura A, Zedda M, Farina V.
Aromatase Immunoreactivity in Fetal Ovine Neuronal Cell Cultures Exposed to Oxidative Injury. ,
Eur J Histochem
 53 , e28 , 2009
Cells
Neurons
Enzymes
PDS kit : per instructions
Medium
DMEM
Epithelial
Fish
Shark (Squalus acanthias)
Karnaky, Jr. , Valentich, J.D. , Currie, M.G. , Oehlenschlager, W.F. and Kennedy, M.P.
Atriopeptin Stimulates Chloride Secretion in Cultured Shark Rectal Gland Cells ,
Am J Physiol
 260 , 1125 , 1991
Cells
Rectal gland
Enzymes
Collagenase : 0.2%
Medium
Ringer's solution
Epithelial
Fish
Shark (Squalus acanthias)
Valentich, J.
Primary Cultures of Shark Rectal Gland Epithelial Cells: A Model for Hormone-Sensitive Chloride Transport ,
J Tiss Cul Meth
 13 , 149 , 1991
Cells
Rectal gland
Enzymes
Collagenase : 0.2%
Medium
Ringer's solution
Epithelial
Fish
Winter flounder, 200-500 g
(Pseudopleuronectes americanus)
Dickman, K. and Renfro, J.
Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport ,
Am J Physiol
 251 , 424 , 1986
Cells
Renal tubule
Enzymes
Trypsin : 0.2%
Trypsin : 0.2%
Medium
CMF solution
Epithelial
Hamster
Hamster, Syrian gold, male, 100-120 g
Niles,R. , Kim, K.C. , Hyman, B. , Christensen, T. , Wasano, K. and Brody, J.
Characterization Of Extended Primary And Secondary Cultures Of Hamster Tracheal Epithelial Cells ,
In Vitro Cell Dev Biol
 24 , 457 , 1988
Cells
Tracheal
Enzymes
Pronase : 0.1%
Medium
MEM with Hepes, CMF
Epithelial
Hamster
Hamster, Syrian golden (strain CR:RGH)
McDowell, E. and et al.
Differentiation of Tracheal Mucociliary Epithelium in Primary Cell Culture Recapitulates Normal Fetal Development and Regeneration Following Injury in Hamsters ,
Am J Pathol
 129 , 511 , 1987
Cells
Tracheal
Enzymes
Trypsin : 0.1%
Medium
Ham's F-12
Epithelial
Hamster
Hamster (strain 1516 EHS and Lakeview), 8-12 weeks
Lee, T. , Wu, R. , Brody, A. , Barrett, J. and Nettesheim, P.
Growth and Differentiation of Hamster Tracheal Epithelial Cells in Culture ,
Exp Lung Res
 6 , 27 , 1984
Cells
Tracheal
Enzymes
Trypsin : 0.05%
Medium
Medium 199
Epithelial
Hamster
Hamster, Syrian gold, male, 6 weeks- 4 months
Goldman, W.E. and Baseman, J.B.
Selective Isolation and Culture of a Proliferating Epithelial Cell Population from the Hamster Trachea ,
In Vitro
 16 , 313 , 1980
Cells
Tracheal
Enzymes
Trypsin : 0.25%
Medium
PBS with EDTA
Eye
Fish

Zebrafish, Danio rerio

Middel, C. , Dietrich, N. , Hammes, H. and Kroll, J.
Analysis of the Morphology of Retinal Vascular Cells in Zebrafish , Front Cell Dev Biol Vol. 11 , 1267232 , 2023
Cells
Retinal vascular
Enzymes
Trypsin : 0.3%
Medium
PBS
Eye
Fish
Fish, zebrafish
Diekmann, H. , Kalbhen, P. and Fischer, D.
Characterization of Optic Nerve Regeneration using Transgenic Zebrafish. ,
Front Cell Neurosci
 9 , 118 , 2015
Cells
Retinal
Enzymes
Papain : 10 u/ml
Medium
L-15
Eye
Fish
Goldfish
Graffe, M. , Zenisek, D. and Taraska, J.
A Marginal Band of Microtubules Transports and Organizes Mitochondria in Retinal Bipolar Synaptic Terminals. ,
J Gen Physiol
 146 , 109-17 , 2015
Cells
Retinal Bipolar
Enzymes
Papain : 30 u/ml
Medium
HEPES
Heart
Fish
Zebrafish
Fowler, E. , Wang, N. , Hezzell, M. , Chanoit, G. , Hancox, J. and Cannell, M.
Arrhythmogenic Late Ca2+ Sparks in Failing Heart Cells and Their Control by Action Potential Configuration ,
Proc Natl Acad Sci U S A
 117 , 2687-2692 , 2020
Cells
Cardiac Myocytes
Enzymes
Trypsin : 0.08%
Protease XIV : 0.005%
Collagenase Type 1 : 0.1-0.2%
Medium
HEPES
Heart
Fish
Fish, Danio rerio
Sander, V. , Sune, G. , Jopling, C. , Morera, C. and Belmonte J.
Isolation and In Vitro Culture of Primary Cardiomyocytes from Adult Zebrafish Hearts. ,
Nat Protoc
 8 , 800-9 , 2013
Cells
Cardiomyocytes
Enzymes
Medium
MEM
Heart
Ovine
Ovine
Voigt, N. , Pearman, C. , Dobrev, D. and Dibb, K.
Methods for Isolating Atrial Cells from Large Mammals and Humans. ,
J Mol Cell Cardiol
 86 , 187-98 , 2015
Cells
Atrial myocytes
Enzymes
Protease XIV : 0.006%
Medium
Tyrode's solution
Heart
Ovine
Ovine
Dibb, K. , Clarke, J. , Horn, M. , Richards, M. , Graham, H. , Eisner, D. and Trafford, A.
Characterization of an Extensive Transverse Tubular Network in Sheep Atrial Myocytes and its Depletion in Heart Failure. ,
Circ Heart Fail
 2 , 482-9 , 2009
Cells
Cardiomyocytes
Enzymes
Protease Type XIV : 0.006%
Medium
See reference
Heart
Ovine
Ovine
Dhamoon, A, Pandit, S. , Sarmast, F. , Parisian, K. , Guha, P. , Li, Y. , Bagwe, S. , Taffet, S. and Anumonwo, J.
Unique Kir2.x Properties Determine Regional and Species Differences in the Cardiac Inward Rectifier K+ Current. ,
Circ Res
 94 , 1332-9 , 2004
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 100-200 u/ml
Medium
See reference
Kidney
Fish
Winter flounder (Pseudopleur-onectes americanus)
200 - 500 g
Dickman, K. and Renfro, J.
Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport ,
Am J Physiol
 251 , 424 , 1986
Cells
Renal tubule
Enzymes
Trypsin : 0.2%
Trypsin : 0.2%
Medium
CMF solution
Kidney
Hamster
Hamster
(also rat, SD and Wistar, 150-225 g, rabbit, bovine)
Husted, R. , Hayashi, M. and Stokes, J.
Characteristics of Papillary Collecting Duct Cells in Primary Culture ,
Am J Physiol
 255 , F1160 , 1988
Cells
Papillary duct
Enzymes
Hyaluronidase : 0.2%
Medium
Keri's buffer
HEPES buffered saline
Liver
Fish
Rainbow trout (Oncorhynchus mykiss), male & female,
120-600 g
Blair, J.B. , Miller, M.R. , Pack, D. , Barnes, R. , Teh, S.J. and Hinton, D.E.
Isolated Trout Liver Cells:Establishing Short-Term Primary Cultures Exhibiting Cell-to-Cell Interactions ,
In Vitro Cell Dev Biol
 26 , 237 , 1990
Cells
Hepatocytes
Enzymes
Medium
Kreb's-Ringer bicarbonate buffer, CF
Liver
Fish
Trout, male, 100 - 200 g
Lipsky, M. , Sheridan, T. , Bennett, R. and May, E.
Comparison of Trout Hepatocyte Culture on Different Substrates ,
In Vitro Cell Dev Biol
 22 , 360 , 1986
Cells
Hepatocytes
Enzymes
Medium
HBSS with 0.05M HEPES
Liver
Fish
Rainbow trout, (Salmo gairdneri), male, 150-200 g
Klaunig, J. , Ruch, R. and Goldblah, P.
Trout Hepatocyte Culture: Isolation and Primary Culture ,
In Vitro Cell Dev Biol
 21 , 221 , 1985
Cells
Hepatocytes
Enzymes
Collagenase : 100 u/ml
Medium
HBSS/CMF
Liver
Fish
Trout 100g - 5kg
Bailey, G. , Taylor, M. and Selivonchick, D.
Aflatoxin B1 Metabolism and DNA Binding in Isolated Hepatocytes From Rainbow Trout Salmo gairdner ,
Carcinogenesis
 3 , 511 , 1982
Cells
Hepatocytes
Enzymes
Hyaluronidase : 0.08%
Medium
See reference
Liver
Fish
Trout (Salmo gairdneri), male, 150-200 g
Catfish (Ictalurus punctatus), male, 100 - 150 g
Klaunig, J.
Establishment of Fish Hepatocyte Cultures For Use in In Vitro Carcinogenicity Studies ,
Natl Cancer Inst Monogr
 65 , 163 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 100u/ml
Medium
HBSS
Muscle
Fish
Zebrafish
Alexander, M. , Kawahara, G. , Kho, A. , Howell, M. , Pusack, T. , Myers, J. , Montanaro, F. , Zon, L. , Guyon, J. and Kunkel, L.
Isolation and Transcriptome Analysis of Adult Zebrafish Cells Enriched for Skeletal Muscle Progenitors. ,
Muscle Nerve
 43 , 741-50 , 2011
Cells
Myogenic progenitor
Enzymes
Medium
PBS
Muscle
Fish
Dogfish, Squalus acanthias , 2-6 Kg
(also guinea-pig, rabbit, frog)
Mitra, R. and Morad, M.
A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates ,
Am J Physiol
 249 , H1056 , 1985
Cells
Myocytes, heart and stomach
Enzymes
Protease XIV : 0.028%
Medium
Solution C
(see reference)
Muscle
Hamster
Hamster, male, 60-70 day
Nakamura, T. , Iwata, Y. , Sampaolesi, M. , Hanada, H. , Saito, N. , Artman, M. , Coetzee, W. and Shigekawa, M.
Stretch-Activated Cation Channels in Skeletal Muscle Myotubes From Sarcoglycan-Deficient Hamsters ,
Am J Physiol Cell Physiol
 281 , C690 , 2001
Cells
Satellite
Enzymes
Trypsin : 0.25%
Medium
DMEM
Neural
Fish
Zebrafish, embryonic
Spiro, Z. , Koh, A. , Tay, S. , See, K. and Winkler, C.
Transcriptional Enhancement of Smn Levels in Motoneurons is Crucial for Proper Axon Morphology in Zebrafish. ,
Sci Rep
 6 , 27470 , 2016
Cells
Motorneurons
Enzymes
PDS kit : per instructions
Medium
L-15

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.