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Tissue References Search Tool

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Heart
Human
Human, fetal, 14.5 week gestation
Goldman, B. and Wurzel, J.
Effects of Subcultivation and Culture Medium on Differentiation of Human Fetal Cardiac Myocytes ,
In Vitro Cell Dev Biol
 28 , 109 , 1992
Cells
Myocytes
Enzymes
Hyaluronidase : 0.1%
Medium
HBSS with Calcium
Heart
Human
Human, both sexes, 18-81 years
Smith, D. , Glover, J. , Townsend, L. and Maupin, E.
A Method for the Harvest, Culture, and Characterization of Human Adult Atrial Myocardial Cells: Correlation with Age of Donor ,
In Vitro Cell Dev Biol
 27 , 914 , 1991
Cells
Myocardial
Atrial
Enzymes
Collagenase : 0.14%
Medium
HBSS, CMF
Heart
Human
Human, adult
Bugaisky, L.B.
Biology of Isolated Adult Cardiac Myocytes , Isolation and Culture of Human Adult Cardiac Myocytes , (Clark,Decker,Borg,eds) , Elsevier Science Publishing Co., Inc. , 1988
Cells
Myocytes
Enzymes
Trypsin : 0.25%
Medium
Joklik's MEM
Heart
Human
Human
Hassler, O. , Wiren, M. and Herbertsson, S.
The Elastic Coat of the Arterial Wall Studied with the Aid of Collagenase ,
Acta Pathol Microbiol Scand
 57 , 15 , 1963
Cells
Thoracic aorta
Enzymes
Collagenase : 0.2%
Medium
Phosphate buffer w/NaCl
Intestine
Human
Human
Trapecar, M. , Khan, S. , Roan, N. , Chen, T. , Telwatte, S. , Deswal, M. , Pao, M. , Somsouk, M. , Deeks, S. , Hunt, P. , Yukl, S. and Sanjabi, S.
An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies. ,
AIDS Res Hum Retroviruses
 33 , S31-S39 , 2017
Cells
Lymphocytes
Enzymes
CLSPA : 0.05%
Deoxyribonuclease I : 0.00075%
Medium
RPMI-1640
Intestine
Human
Human
Kessler, S. , Rho, H. , West, G. , Fiocchi, C. , Drazba, J. and de la Motte, C.
Hyaluronan (HA) Deposition Precedes and Promotes Leukocyte Recruitment in Intestinal Inflammation. ,
Clin Transl Sci
 1 , 57-61 , 2008
Cells
Microvessel endothelial
Enzymes
Medium
HBSS
Intestine
Human
Human
Ebert, Ellen.
CD2 Activation of Human Lamina Propria Lymphocytes Reduces CD3 Responsiveness. ,
Immunology
 117 , 71-7 , 2006
Cells
Lamina propria lymphocytes
Enzymes
Collagenase : 20 u/ml
Medium
Medium
Intestine
Human
Human
Kanai Takanori, Totsuka Teruji, Uraushihara Koji, Makita Shin, Nakamura Tetsuya, Koganei Kazutaka, Fukushima Tsuneo, Akiba Hisaya, Yagita Hideo, Okumura Ko, Machida Utako, Iwai Hideyuki, Azuma Miyuki, Chen Lieping, Watanabe Mamoru.
Blockade of B7-H1 suppresses the development of chronic intestinal inflammation ,
J Immunol
 171 , 4156-63 , 2003
Cells
Lamina propria mononuclear cells
Enzymes
Collagenase : 0.02%
Medium
HBSS
Intestine
Human
Human
Fahlgren, A. , Hammarstrom, S. , Danielsson, A. and Hammarstrom, M.
Increased Expression of Antimicrobial Peptides and Lysozyme in Colonic Epithelial Cells of Patients with Ulcerative Colitis. ,
Clin Exp Immunol
 131 , 90 , 2003
Cells
Intestinal epithelial
Enzymes
Collagenase Type 4 : 72.5 u/ml
Medium
HBSS
Intestine
Human
Human
Stallmach A, Schaffer F, Hoffmann S, Weber S, Muller-Molaian I, Schneider T, Kohne G, Ecker KW, Feifel G, Zeitz M.
Increased state of activation of CD4 positive T cells and elevated interferon gamma production in pouchitis ,
Gut
 43 , 499-505 , 1998
Cells
Mucosal mononuclear cells
Enzymes
Medium
RPMI
Kidney
Fish
Winter flounder (Pseudopleur-onectes americanus)
200 - 500 g
Dickman, K. and Renfro, J.
Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport ,
Am J Physiol
 251 , 424 , 1986
Cells
Renal tubule
Enzymes
Trypsin : 0.2%
Trypsin : 0.2%
Medium
CMF solution
Kidney
Human
Human
Basu, J. , Genheimer, C. , Sangha, N. , Quinlan, S. , Guthrie, K. , Kelley, R. , Ilagan, R. , Jain, D. , Bertram, T. and Ludlow, J.
Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. ,
Lipids Health Dis
 10 , 171 , 2011
Cells
Renal adipose derived cells
Enzymes
Medium
DMEM
Kidney
Human
Human, adult
Valente, M. , Henrique, R. , Costa, V. , Jeronimo, C. , Carvalho, F. , Bastos, M. , Guedes de Pinho, P. and Carvalho, M.
A Rapid and Simple Procedure for the Establishment of Human Normal and Cancer Renal Primary Cell Cultures from Surgical Specimens. ,
PLoS ONE
 6 , e19337 , 2011
Cells
Renal tumor cells and proximal tubular epithelial
Enzymes
Medium
DMEM/F12
Kidney
Human
Human
Presnell, S. , Bruce, A. , Wallace, S. , Choudhury, S. , Genheimer, C. , Cox, B. , Guthrie, K. , Werdin, E. , Tatsumi-Ficht, P. , Ilagan, R. , Kelley, R. , Rivera, E. , Ludlow, J. , Wagner, B. , Jayo, M. and Bertram, T.
Isolation, Characterization, and Expansion Methods for Defined Primary Renal Cell Populations from Rodent, Canine, and Human Normal and Diseased Kidneys. ,
Tiss Eng
 17 , 261 , 2011
Cells
Renal cells
Enzymes
Collagenase Type 4 : 300 u/ml
Neutral Protease : see reference
Medium
HBSS
Kidney
Human
Human, adult
Johnson, D. , Saunders, H. , Johnson, F. , Huq, S. , Field, M. and Pollock, C.
Cyclosporin exerts a direct fibrogenic effect on human tubulointerstitial cells: roles of insulin-like growth factor I, transforming growth factor beta1, and platelet-derived growth factor ,
J Pharmacol Exp Ther
 289 , 535-42 , 1999
Cells
Renal proximal tubule and cortical fibroblasts
Enzymes
Collagenase Type 2 : 383 u/ml
Medium
DMEM/F-12
Kidney
Human
Human
McAteer, J, Kempson, S. and Evan, A
Culture of Human Renal Cortex Epithelial Cells ,
J Tiss Cul Meth
 13 , 143 , 1991
Cells
Renal cortex
Enzymes
Trypsin : 0.1%
Medium
Tissue Culture Grade Water
Kidney
Human
Human
Trifillis, A. and Kahng, M.
Characterization of an In Vitro System of Human Renal Papillary Collecting Duct Cells ,
In Vitro Cell Dev Biol
 26 , 441 , 1990
Cells
Papillary duct
Enzymes
Collagenase : 400 u/ml
Medium
Eagle's MEM-HEPES buffer w/L-glutamine
Kidney
Human
Human
Heieren, M. , van der Woude, F. and Balfour Jr., H.
Cytomegalovirus Replicates Efficiently in Human Kidney Mesangial Cells ,
Proc Natl Acad Sci U S A
 85 , 1642 , 1988
Cells
Mesangial
Enzymes
Trypsin : 0.25%
Medium
DMEM/Ham's F-12
Kidney
Human
Human, adult
Yang, A. , Gould-Kostka, J. and Oberley, T.
In Vitro Growth and Differentiation of Human Kidney Tubular Cells on a Basement Membrane Substrate ,
In Vitro Cell Dev Biol
 23 (1) , 34 , 1987
Cells
Tubular
Enzymes
Collagenase : 250 u/ml
Medium
PBS
Kidney
Human
Human, adult, 14-66 years
Trifillis, A. , Regec, A. and Trump, B.
Isolation, Culture, and Charaterization of Human Renal Tubular Cells ,
J Urol
 133 , 324 , 1985
Cells
Tubular
Enzymes
Collagenase : 100 u/ml
Medium
Joklik's MEM
Kidney
Human
Human
Hemstreet, G. , Enoch, P. and Pretlow, T.
Tissue Disaggregation of Human Renal Cell Carcinoma with Further Isopyknic and Isokinetic Gradient Purification ,
Cancer Res
 40 , 1043 , 1980
Cells
Malignant
Stromal
Enzymes
Papain : 0.009%
Medium
Sacks solution
Kidney
Human
Human, 24 newborn and stillborn
(also rabbit)
De Oca, H. and Malinin, T.
Dispersion and Cultivation of Renal Cells After Short-Term Storage of Kidneys ,
J Clin Microbiol
 2 , 243 , 1975
Cells
Renal
Enzymes
Trypsin : 0.25%
Medium
See reference
Liver
Fish
Rainbow trout (Oncorhynchus mykiss), male & female,
120-600 g
Blair, J.B. , Miller, M.R. , Pack, D. , Barnes, R. , Teh, S.J. and Hinton, D.E.
Isolated Trout Liver Cells:Establishing Short-Term Primary Cultures Exhibiting Cell-to-Cell Interactions ,
In Vitro Cell Dev Biol
 26 , 237 , 1990
Cells
Hepatocytes
Enzymes
Medium
Kreb's-Ringer bicarbonate buffer, CF
Liver
Fish
Trout, male, 100 - 200 g
Lipsky, M. , Sheridan, T. , Bennett, R. and May, E.
Comparison of Trout Hepatocyte Culture on Different Substrates ,
In Vitro Cell Dev Biol
 22 , 360 , 1986
Cells
Hepatocytes
Enzymes
Medium
HBSS with 0.05M HEPES
Liver
Fish
Rainbow trout, (Salmo gairdneri), male, 150-200 g
Klaunig, J. , Ruch, R. and Goldblah, P.
Trout Hepatocyte Culture: Isolation and Primary Culture ,
In Vitro Cell Dev Biol
 21 , 221 , 1985
Cells
Hepatocytes
Enzymes
Collagenase : 100 u/ml
Medium
HBSS/CMF
Liver
Fish
Trout 100g - 5kg
Bailey, G. , Taylor, M. and Selivonchick, D.
Aflatoxin B1 Metabolism and DNA Binding in Isolated Hepatocytes From Rainbow Trout Salmo gairdner ,
Carcinogenesis
 3 , 511 , 1982
Cells
Hepatocytes
Enzymes
Hyaluronidase : 0.08%
Medium
See reference
Liver
Fish
Trout (Salmo gairdneri), male, 150-200 g
Catfish (Ictalurus punctatus), male, 100 - 150 g
Klaunig, J.
Establishment of Fish Hepatocyte Cultures For Use in In Vitro Carcinogenicity Studies ,
Natl Cancer Inst Monogr
 65 , 163 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 100u/ml
Medium
HBSS
Liver
Human

Human

Kucykowicz, S. , Amin, O. , Burton, A. , Swadling, L. , Schmidt, N. , Zakeri, N. , Davies, J. , Aidoo-Micah, G. , Stegmann, K. , Easom, N. , Jeffery-Smith, A. , Maini, M. and Pallett, L.
Isolation of Human Intrahepatic Leukocytes for Phenotypic and Functional Characterization by Flow Cytometry. ,
Star Protocols
 3 , 101356 , 2022
Cells
Intrahepatic leukocytes
Enzymes
Medium
RPMI-1640
Liver
Human
Human
Reuschel, E, Jilg, W. , Seelbach-Goebel, B. and Demi, L.
Comparative Purification and Characterization of Hepatitis B Virus-like Particles Produced by Recombinant Vaccinia Viruses in Human Hepatoma Cells and Human Primary Hepatocytes ,
PLoS ONE
 , 2019
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Human
Human
Pfeiffer, E. , Kegel, V. , Zeilinger, K. , Hengstler, J. , Nussler, A. , Seehofer, D. and Damm, G.
Featured Article: Isolation, Characterization, and Cultivation of Human Hepatocytes and Non-Parenchymal Liver Cells. ,
Exp Biol Med (Maywood)
 240 , 645-56 , 2015
Cells
Hepatocytes and non-parenchymal cells
Enzymes
Medium
HBSS
Liver
Human
Human
Werner, M. , Driftmann, S. , Kleinehr, K. , Kaiser, G. , Mathe, Z. , Treckmann, J. , Paul, A. , Skibbe, K. , Timm, J. , Canbay, A. , Gerken, G. , Schlaak, J. and Broering, R.
All-In-One: Advanced Preparation of Human Parenchymal and Non-Parenchymal Liver Cells. ,
PLoS ONE
 10 , e0138655 , 2015
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Human
Human
Lee, S. , Schelcher, C. , Demmel, M. , Hauner, M. and Thasler, W.
Isolation of Human Hepatocytes by a Two-Step Collagenase Perfusion Procedure. ,
J Vis Exp
 , 2013
Cells
Hepatocytes
Enzymes
Collagenase : 0.1-0.15%
Medium
HEPES
Liver
Human
Human, fetal
Vatakis, D. , Bristol, G. , Kim, S. , Levin, B. , Liu, W. , Radu, C. , Kitchen, S. and Zack, J.
Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model. ,
J Vis Exp
 70 , e4181 , 2012
Cells
Liver hematopoietic
Enzymes
Medium
RPMI
Liver
Human
Human
Torok, E. , Lutgehetmann, M. , Bierwolf, J. , Melbeck, S. , Dullmann, J. , Nashan, B. , Ma, P. and Pollok, J.
Primary Human Hepatocytes on Biodegradable Poly(l-lactic acid) Matrices: A Promising Model for Improving Transplantation Efficiency with Tissue Engineering. ,
Liver Transpl
 17 , 104 , 2011
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Human
Human
Wauthier, E. , Schmelzer, E. , Turner, W. , Zhang, L. , LeCluyse, E. , Ruiz, J. , Turner, R. , Furth, M. , Kubota, H. , Lozoya, O. , Barbier, C. , McClelland, R. , Yao, H. , Moss, N. , Bruce, A. , Ludlow, J. and Reid, L.
Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance. ,
Methods Cell Biol
 86 , 137 , 2008
Cells
Hepatic stem cells and heptoblasts
Enzymes
Collagenase Type 4 : 0.014-0.06%
Medium
various
Liver
Human
Human
Pichard L, Raulet E, Fabre G, Ferrini JB, Ourlin JC. and Maurel P
Human hepatocyte culture ,
Methods Mol Biol
 320 , 283 , 2006
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HEPES

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.