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Tissue References Search Tool

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Adipose/Fat
Avian
Scherpelz, K. , Lu, J.,Tycko, R. and Meredith, S.
Preparation of Amyloid Fibrils Seeded from Brain and Meninges ,
Methods Mol Biol
 1345 , 299 , 2016
Cells
Amyloid Fibrils
Enzymes
Medium
50mM Tris
Adipose/Fat
Avian
Scherpelz, K. , Lu, J.,Tycko, R. and Meredith, S.
Preparation of Amyloid Fibrils Seeded from Brain and Meninges ,
Methods Mol Biol
 1345 , 299 , 2016
Cells
Amyloid Fibrils
Enzymes
Medium
50mM Tris
Adrenal
Ovine
Ovine, adult and fetal
Valego, N. and Rose, J.
A Specific CRH Antagonist Attenuates ACTH-Stimulated Cortisol Secretion in Ovine Adrenocortical Cells. ,
Reprod Sci
 17 , 477 , 2010
Cells
Adrenocortical
Enzymes
Medium
DMEM/Ham's F12
Adrenal
Ovine
Ovine, fetal
Valego, N. , Su, Y. , Carey, L. , Young, S. , Tatter, S. , Wang, J. and Rose, J.
Hypothalamic-Pituitary Disconnection in Fetal Sheep Blocks the Peripartum Increases in Adrenal Responsiveness and Adrenal ACTH Receptor Expression. ,
Am J Physiol Regul Integr Comp Physiol
 289 , R410 , 2005
Cells
Adrenocortical
Enzymes
Medium
DMEM/Ham's F12
Adrenal
Ovine
Ovine, adult
Keating, D. , Rychkov, G. , Adams, M. , Holgert, H. , McMillen, I.C. and Roberts, M.
Opioid Receptor Stimulation Suppresses the Adrenal Medulla Hypoxic Response in Sheep by Actions on Ca(2+) and K(+) Channels. ,
J Physiol
 555 , 489 , 2004
Cells
Chromaffin cells
Enzymes
Medium
Locke's solution
Adrenal
Ovine
Ovine, 3 year
Canny B J, O'Farrell K A, Clarke I J, Tilbrook A J.
The influence of sex and gonadectomy on the hypothalamo-pituitary-adrenal axis of the sheep ,
J Endocrinol
 162 , 215-25 , 1999
Cells
Anterior pituitary
Enzymes
Trypsin : 2.5%
Medium
DMEM
Brain
Ovine
Ovine
Reddy, R. , Amodei, R. , Estill, C. , Stormshak, F. , Meaker, M. and Roselli, C.
Effect of Testosterone on Neuronal Morphology and Neuritic Growth of Fetal Lamb Hypothalamus-Preoptic Area and Cerebral Cortex in Primary Culture. ,
PLoS ONE
 10 , e0129521 , 2015
Cells
Neurons
Enzymes
Papain : 0.2%
Medium
Hibernate
Brain
Ovine
Ovine
Lepore G, Gadau S, Mura A, Zedda M, Farina V.
Aromatase Immunoreactivity in Fetal Ovine Neuronal Cell Cultures Exposed to Oxidative Injury. ,
Eur J Histochem
 53 , e28 , 2009
Cells
Neurons
Enzymes
PDS kit : per instructions
Medium
DMEM
Cartilage
Goat

Goat

Burdis, R. , Chariyev-Prinz, F. , Browe, D. , Freeman, F. , Nulty, J. , McDonnell, E. , Eichholz, K. , Wang, B. , Brama, P. and Kelly, D.
Spatial Patterning of Phenotypically Distinct Microtissues to Engineer Osteochondral Grafts for Biological Joint Resurfacing. ,
Biomaterials
 289 , 121750 , 2022
Cells
Chondrocytes
Enzymes
Collagenase Type 2 : 350 u/ml
Medium
DMEM
Cartilage
Goat
Goat
Bekkers,J, Creemers, L, Tsuchida, A, van Rijen, M, Custers, R, Dhert, W. and Saris, D.
One-Stage Focal Cartilage Defect Treatment with Bone Marrow Mononuclear Cells and Chondrocytes Leads to Better Macroscopic Cartilage Regeneration Compared to Microfracture in Goats. ,
Osteoarthritis Cartilage
 21 , 950-6 , 2013
Cells
Chondrocytes
Enzymes
Medium
DMEM
Heart
Ovine
Ovine
Voigt, N. , Pearman, C. , Dobrev, D. and Dibb, K.
Methods for Isolating Atrial Cells from Large Mammals and Humans. ,
J Mol Cell Cardiol
 86 , 187-98 , 2015
Cells
Atrial myocytes
Enzymes
Protease XIV : 0.006%
Medium
Tyrode's solution
Heart
Ovine
Ovine
Dibb, K. , Clarke, J. , Horn, M. , Richards, M. , Graham, H. , Eisner, D. and Trafford, A.
Characterization of an Extensive Transverse Tubular Network in Sheep Atrial Myocytes and its Depletion in Heart Failure. ,
Circ Heart Fail
 2 , 482-9 , 2009
Cells
Cardiomyocytes
Enzymes
Protease Type XIV : 0.006%
Medium
See reference
Heart
Ovine
Ovine
Dhamoon, A, Pandit, S. , Sarmast, F. , Parisian, K. , Guha, P. , Li, Y. , Bagwe, S. , Taffet, S. and Anumonwo, J.
Unique Kir2.x Properties Determine Regional and Species Differences in the Cardiac Inward Rectifier K+ Current. ,
Circ Res
 94 , 1332-9 , 2004
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 100-200 u/ml
Medium
See reference
Kidney
Avian
Avian, house sparrows
Goldstein D. , Reddy V. and Plaga K.
Second Messenger Production in Avian Medullary Nephron Segments in Response to Peptide Hormones ,
Am J Physiol
 276 , R847 , 1999
Cells
Tubule segments
Enzymes
Medium
HBSS
Kidney
Avian
Avian, chicken
Reich, C. and Bonar, R.
Separation of Avian Kidney Tubules and Glomeruli for In Vitro Culture ,
Meth Cell Sci
 7 , 97 , 1982
Cells
Tubule and glomeruli
Enzymes
Medium
RPMI 1640
Liver
Avian
Avian, various
Else, P. , Brand, M. , Turner, N. and Hulbert, A
Respiration Rate of Hepatocytes Varies With Body Mass in Birds ,
J Exp Biol
 207 , 2305 , 2004
Cells
Hepatocytes
Enzymes
Medium
see reference
Liver
Avian
Ducklings, 7 day
Lee J. , Culvenor J. , Angus P. , Smallwood R. , Nicoll A. and Locarnini S.
Duck Hepatitis B Virus Replication in Primary Bile Duct Epithelial Cells ,
J Virol
 75(16) , 7651 , 2001
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Hyaluronidase : 0.05%
Medium
DMEM/ F12
Mammary
Goat
Goat, lactating, 1 month
Blanco, A. , Rife, U. and Larson, B.
Lactate Dehydrogenase Isozymes during Dedifferentiation in Cultures of Mammary Secretory Cells ,
Nature
 214 , 1331 , 1967
Cells
Mammary gland
Enzymes
Collagenase : 0.02 - 0.03%
Medium
HBSS or EBSS
Miscellaneous
Goat
Goat
Arora, A. , Sriram, M. , Kothari, A. and Katti, D.
Co-culture of Infrapatellar Fat Pad-Derived Mesenchymal Stromal Cells and Articular Chondrocytes in Plasma Clot for Cartilage Tissue Engineering. ,
Cytotherapy
 19 , 881-894 , 2017
Cells
Infrapatellar fat pad mesenchymal stromal
Enzymes
Collagenase : 0.15%
Medium
DMEM
Neural
Avian
Quail and Chicken, embryonic
Rollo, B. , Zhang, D. , Simkin, J. , Menheniott, T. and Newgreen, D.
Why are Enteric Ganglia so Small? Role of Differential Adhesion of Enteric Neurons and Enteric Neural Crest Cells. ,
F1000Res
 4 , 113 , 2015
Cells
Midgut DRG
Enzymes
Medium
Ham's F12
Neural
Avian
Chick, White Leghorn, embryos
Temburni, M. , Rosenberg, M. , Pathak, N. , McConnell, R. and Jacob, M.
Neuronal Nicotinic Synapse Assembly Requires the Adenomatous Polyposis Coli Tumor Suppressor Protein. ,
J Neurosci
 24 , 6776 , 2004
Cells
Cilary ganglion neurons
Enzymes
Medium
PBS
Pituitary
Ovine
Ovine, adult
Xu Ruwei, Wang Qinling, Yan Ming, Hernandez Maria, Gong Changhong, Boon WahChin, Murata Yoko, Ueta Yoichi, Chen Chen.
Orexin-A augments voltage-gated Ca2+ currents and synergistically increases growth hormone (GH) secretion with GH-releasing hormone in primary cultured ovine somatotropes ,
Endocrinology
 143 , 4609-19 , 2002
Cells
Somatotropes
Enzymes
Medium
Medium 199
Reproductive
Ovine
Ovine, 3-5 year
Letouzey, V. , Tan, K. , Deane, J. , Ulrich, D. , Gurung, S. , Ong, Y. and Gargett, C.
Isolation and Characterisation of Mesenchymal Stem/Stromal Cells in the Ovine Endometrium. ,
PLoS ONE
 10 , e0127531 , 2015
Cells
Endometrial stromal
Enzymes
Medium
DMEM/F-12
Skin
Goat
Goat
Deng, Y. , Hu, J. and Athanasiou, K.
Isolation and Chondroinduction of a Dermis-Isolated, Aggrecan-Sensitive Subpopulation with High Chondrogenic Potential. ,
Arthritis Rheum
 56 , 168 , 2007
Cells
Dermis isolated aggrecan sensitive
Enzymes
Medium
DMEM
Stem
Ovine
Ovine, 3-5 year
Letouzey, V. , Tan, K. , Deane, J. , Ulrich, D. , Gurung, S. , Ong, Y. and Gargett, C.
Isolation and Characterisation of Mesenchymal Stem/Stromal Cells in the Ovine Endometrium. ,
PLoS ONE
 10 , e0127531 , 2015
Cells
Endometrial stromal
Enzymes
Medium
DMEM/F-12

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.