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Tissue References Search Tool

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Lung
Mouse
Mouse
Paine R 3rd, Wilcoxen SE, Morris SB, Sartori C, Baleeiro CE, Matthay MA. and Christensen PJ
Transgenic overexpression of granulocyte macrophage-colony stimulating factor in the lung prevents hyperoxic lung injury ,
Am J Pathol
 163 , 2397 , 2003
Cells
Alveolar epithelial cells
Enzymes
Medium
DMEM
Lung
Mouse
Mouse, 6-10 week
Andonegui G. , Bonder C. , Green F. , Mullaly S. , Zbytnuik L. , Raharjo E. and Kubes P.
Endothelium-derived Toll-like Receptor-4 is the Key Molecule in LPS-induced Neutrophil Sequestration into Lungs ,
J Clin Invest
 111 , 1011 , 2003
Cells
Murine pulmonary endothelial
Enzymes
Medium
HBSS
Lung
Mouse
Mouse
Huaux F, Liu T, McGarry B, Ullenbruch M. and Phan SH.
Dual roles of IL-4 in lung injury and fibrosis ,
J Immunol
 170 , 2083 , 2003
Cells
Pulmonary T lymphocytes
Enzymes
Collagenase Type 3 : 10 mg/lung
Deoxyribonuclease I : 250 ug/lung
Medium
RPMI medium
Lung
Mouse
Mouse, 6-8 week
Vermaelen KY, Carro-Muino I, Lambrecht BN. and Pauwels RA.
Specific migratory dendritic cells rapidly transport antigen from the airways to the thoracic lymph nodes ,
J Exp Med
 193 , 51 , 2001
Cells
Lung and lymph node cells
Enzymes
Medium
RPMI 1640
Lung
Mouse
Mouse
Freedman SD, Katz MH, Parker EM, Laposata M, Urman MY. and Alvarez JG.
A membrane lipid imbalance plays a role in the phenotypic expression of cystic fibrosis in cftr(-/-) mice ,
Proc Natl Acad Sci U S A
 96 , 13995 , 1999
Cells
Lung
Enzymes
Collagenase : 100 u/ml
Deoxyribonuclease I : 200 u/ml
Medium
Krebs-Henseleit Buffer
Lung
Mouse
Mouse, 6-8 wk
Stampfli MR, Wiley RE, Neigh GS, Gajewska BU, Lei XF, Snider DP, Xing Z, Jordana M.
GM-CSF transgene expression in the airway allows aerosolized ovalbumin to induce allergic sensitization in mice ,
J Clin Invest
 102 , 1704-14 , 1998
Cells
Lung cells
Enzymes
Collagenase Type 3 : 150 u/ml
Medium
HBSS
Lung
Mouse
Mouse, female, 18-20 g
Dong QG, Bernasconi S, Lostaglio S, De Calmanovici RW, Martin-Padura I, Breviario F, Garlanda C, Ramponi S, Mantovani A,and Vecchi A.
A general strategy for isolation of endothelial cells from murine tissues. Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants ,
Arterioscler Thromb Vasc Biol
 17 , 1599 , 1997
Cells
Murine endothelial cells
Enzymes
Collagenase Type 1 : 1 mg/ml
Medium
DMEM
Lung
Mouse
Mouse, female
Hamilton-Easton A. and Eichelberger M
Virus-specific antigen presentation by different subsets of cells from lung and mediastinal lymph node tissues of influenza virus-infected mice ,
J Virol
 69 , 6359 , 1995
Cells
Antigen presenting cells
Enzymes
Collagenase : 150 u/ml
Medium
RPMI 1640
Lymph nodes
Mouse
Mouse
Terhorst, D. , Chelbi, R. , Wohn, C. , Malosse, C. , Tamoutounour, S. , Jorquera, A. , Bajenoff, M. , Dalod, M. , Malissen, B. and Henri, S.
Dynamics and Transcriptomics of Skin Dendritic Cells and Macrophages in an Imiquimod-Induced, Biphasic Mouse Model of Psoriasis. ,
J Immunol
 195 , 4953-61 , 2015
Cells
Dendritic
Enzymes
Medium
RPMI 1640
Lymph nodes
Mouse
Mouse
Dubrot, J. , Duraes, F. , Potin, L. , Capotosti, F. , Brighouse, D. , Suter, T. , LeibundGut-Landmann, S. , Garbi, N. , Reith, W. , Swartz, M. and Hugues, S.
Lymph Node Stromal Cells Acquire Peptide-MHCII Complexes from Dendritic Cells and Induce Antigen-Specific CD4+T Cell Tolerance ,
J Exp Med
 211 , 1153 , 2014
Cells
Stromal
Enzymes
Medium
RPMI
Lymph nodes
Mouse
Mouse
Rayamajhi, M. , Redente, E. , Condon, T. , Gonzalez-Juarrero, M. , Riches, D. and Lenz, L.
Non-Surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry. ,
J Vis Exp
 51 , 2702 , 2011
Cells
Lung draining
Enzymes
Collagenase Type 4 : 0.1-0.125%
Medium
HBSS
Lymph nodes
Mouse
Mouse
Kapasi ZF, Qin D, Kerr WG, Kosco-Vilbois MH, Shultz LD, Tew JG, Szakal AK.
Follicular dendritic cell (FDC) precursors in primary lymphoid tissues ,
J Immunol
 160 , 1078-84 , 1998
Cells
Follicular dentritic
Enzymes
Medium
HBSS
Mammary
Guinea-Pig
Guinea-pig, pregnant, 4-10 day
Turba, F. and Hilpert, N.
Secretion and Resorption of Proteins by Isolated Mammary Gland Cells. German ,
Biochem Z
 334 , 501 , 1961
Cells
Mammary gland
Enzymes
Medium
Dulbecco phosphate
Mammary
Mouse

Mouse, female

Sun, H. , Xu, X. and Deng, C.
Preparation of Single Epithelial Cells Suspension from Mouse Mammary Glands. ,
Bio Protoc
 10 , e3530 , 2020
Cells
Mammary epithelial
Enzymes
Medium
DMEM/F-12
Mammary
Mouse
Mouse
Liu, X. , Johnson, S. , Liu, S. , Kanojia, D. , Yue, W. , Singh, U. , Wang, Q, Wang Qi, Nie, Q. and Chen H.
Nonlinear Growth Kinetics of Breast Cancer Stem Cells: Implications for Cancer Stem Cell Targeted Therapy. ,
Sci Rep
 3 , 2473 , 2013
Cells
Mammary tumor
Enzymes
Collagenase : 0.15%
Hyaluronidase : 0.020%
Medium
DMEM/F12
Mammary
Mouse
Mouse, 10 week
Cheng, N. and Lambert, D.
Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice. ,
J Vis Exp
 54 , e2716 , 2011
Cells
Fibroblasts, carcinoma
Enzymes
Trypsin : 0.2%
Hyaluronidase : 1,000 u/ml
Medium
DMEM
Mammary
Mouse
Mouse, 12 week
Taddei, I. , Deugnier, M. , Faraldo, M. , Petit, V. , Bouvard, D. , Medina, D. , Fassler, R. , Thiery, J. and Glukhova, M.
Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells ,
Nat Cell Biol
 10 , 716-22 , 2008
Cells
Mammary epithelial
Enzymes
Collagenase : 0.3%
Hyaluronidase : 100 u/ml
Trypsin : 0.25%
Medium
see reference
Mammary
Mouse
Mouse, 11 week
Mueller, S. , Clark, J. , Myers, P. and Korach, K.
Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
 143 , 2357 , 2002
Cells
Epithelial
Enzymes
Collagenase Type 3 : 25 u/ml
Hyaluronidase : 0.1%
Protease XIV : 0.05%
Medium
DMEM/F12
Mammary
Mouse
Mouse
Boulanger CA, Smith GH.
Reducing mammary cancer risk through premature stem cell senescence ,
Oncogene
 20 , 2264-72 , 2001
Cells
Mammary epithelial stem
Enzymes
Hyaluronidase : 0.1%
Pronase : 1.25%
Medium
DMEM
Mammary
Mouse
Mouse, BALB/C
Kanazawa, T. and Hosick, H.
Transformed Growth Phenotype of Mouse Mammary Epithelium in Primary Culture Induced by Specific Fetal Mesenchymes ,
J Cell Physiol
 153 , 381 , 1992
Cells
Epithelium
Enzymes
Collagenase : 250 u/ml
Medium
HBSS
Mammary
Mouse
Mouse, BALB/c, virgin, female, 4 month
Beck, J. , Hosick, H. and Watkins, B.
Growth of Epithelium From a Preneoplastic Mammary Outgrowth on Response to Mammary Adipose Tissue ,
In Vitro Cell Dev Biol
 25 (5) , 409 , 1989
Cells
Adipocytes
Enzymes
Trypsin : 50 µg/ml
Medium
DMEM
Mammary
Mouse
Mouse (BALB/cCrgl), mature
Bandyopadhyay, G. , Imagawa, W. , Wallace, D. and Nandi, S.
Proliferative effects of insulin and epidermal growth factor on mouse mammary epithelial cells in primary culture ,
J Biol Chem
 263 , 7567 , 1988
Cells
Epithelial
Enzymes
Pronase : 0.01%
Medium
Hepes buffered medium 199
Mammary
Mouse
Mouse, BALB/c, female, 6-8 week
Ehmann, U. , Peterson, W. and Misfeldt, D.
To Grow Mouse Mammary Epithelial Cells in Culture ,
J Cell Biol
 98 , 1026 , 1984
Cells
Epithelial
Enzymes
Medium
DMEM
Mammary
Mouse
Mouse (C3H/HeN), female
Taketani, Y. and Oka, T.
EGF Stimulates Cell Proliferation and Inhibits Functional Differentiation of Mouse Mammalian Dipithelial Cells in Culture ,
Endocrinology
 113 , 871 , 1983
Cells
Epithelial
Enzymes
Medium
Medium 199
Mammary
Mouse
Mouse, (BALB/c/Crgl Me), female, pregnant
Riser, M. , Huff, B. and Medina, D.
Pepsin Can Be Used To Subculture Viable Mammary Epithelial Cells ,
In Vitro
 19 , 730 , 1983
Cells
Epithelial
Enzymes
Pepsin : 0.1% and 0.05%
Pepsin : 0.1%
Medium
HBSS with 0.2% EDTA, CMF
Mammary
Mouse
Mouse, female
Jones, W. and Hallowes, R.
Isolation of the Epithelial Subcomponents of the Mouse Mammary Gland for Tissue-Level Culture Studies ,
J Tiss Cul Meth
 8 (1) , 17 , 1983
Cells
Epithelial
Enzymes
Medium
DMEM
Mammary
Mouse
Mouse (NMuMG), female, 2 months
Ehmann, U. and Misfeldt, D.
Mouse Mammary Cells in D-Valine Medium ,
In Vitro
 18 , 407 , 1982
Cells
Epithelial
Enzymes
Medium
DMEM
Mammary
Mouse
Mouse, BALB/cCr1, female
Asch, B. , Burstein, N. , Vidrich, A. and Sun, T.
Identification of Mouse Mammary Epithelial Cells by Immunofluorescence With Rabbit and Guinea-Pig Antikeratin Antisera ,
Proc Natl Acad Sci U S A
 78 , 5643 , 1981
Cells
Epithelial
Enzymes
Medium
HBSS/DMEM
Mammary
Mouse
Mouse (BALB/cfC3H)
Yang, J. , Guzman, R. , Richards, J. and Nandi, S.
Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels ,
In Vitro
 16 , 502 , 1980
Cells
Mammary tumors
Epithelial
Enzymes
Collagenase : 1.0%
Medium
HBSS
Mammary
Mouse
Mouse, pregnant 8-12 day
Yang J, Richards J, Guzman R, Imagawa W. and Nandi S.
Sustained growth in primary culture of normal mammary epithelial cells embedded in collagen gels ,
Proc Natl Acad Sci U S A
 77 , 2088 , 1980
Cells
Mammary
Medium
HBSS
Mammary
Mouse
Mouse (BALB/c), pregnant, 60-80 days
Ceriani, R. , Peterson, J. and Abraham, S.
Removal of Cell Surface Material by Enzymes Used to Dissociate Mammary Gland Cells ,
In Vitro
 14 , 887 , 1978
Cells
Mammary
Enzymes
Hyaluronidase : 0.1%
Medium
CMF medium
Mammary
Mouse
Mouse, BALB/cfC3H/Crgl, virgin, female
DeOme, K. , Miyamoto, M. , Osborn, R. , Guzman, R. and Lum, K.
Detection of Inaparent Nodule-transformed Cells in the Mammary Gland Tissues of Virgn Female BALB/cfC3H Mice ,
Cancer Res
 38 , 2103 , 1978
Cells
Nodule-transformed
Enzymes
Hyaluronidase : 0.1%
Medium
Medium 199
Mammary
Mouse
Mouse (BALB/cCrgl), virgin, female, 4-8 month
White, M. , Hu, A. , Hamamoto, S. and Nandi
In vitro analysis of proliferating epithelial cell populations from the mouse mammary gland: fibroblast-free growth and serial passage ,
In Vitro
 14 , 271 , 1978
Cells
Epithelial
Mammary gland
Enzymes
Medium
HBSS
Mammary
Mouse
Mouse, BALB/cCrgl, female
(also guinea-pig)
Asch, B. and Medina, D.
Concanavalin A-Induced Agglutinability of Normal, Preneoplastic, and Neoplastic Mouse Mammary Cells ,
J Natl Cancer Inst
 61 (6) , 1423 , 1978
Cells
Swiss 3T3
Enzymes
Medium
PBS CMF
Mammary
Mouse
Mouse, (C3H/Crgl or BALB/cCrgl), 8-10 day pregnant
Emerman, J. , Enami, J. , Pitelka, D. and Nandi, S.
Hormonal Effects on Intracellular and Secreted Casein in Cultures of Mouse Mammary Epithelial Cells on Floating Collagen Membranes ,
Proc Natl Acad Sci U S A
 74 (10) , 4466 , 1977
Cells
Epithelial
Enzymes
Medium
HBSS
Mammary
Mouse
Mouse, lactating
Kerkof, P. and Abraham, S.
Preparation of Adipose Cell-Free Suspensions of Mammary Gland Parenchymal Cells from Lactating Mice ,
Methods Enzymol
 69 , 693 , 1976
Cells
Parenchymal
Enzymes
Medium
Kreb's Ringer bicarbonate buffer

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.