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Tissue References Search Tool

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Heart
Guinea-Pig
Guinea pig, adult
Zorn-Pauly, K. , Schaffer, P. , Pelzmann, B. , Bernhart, E. , Lang, P. and Koidl, B.
L-type and T-type Ca2+ current in cultured ventricular guinea pig myocytes ,
Physiol Res
 53(4) , 369 , 2004
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 100 u/ml
Medium
M-199
Heart
Guinea-Pig
Guinea-Pig
Dhamoon, A, Pandit, S. , Sarmast, F. , Parisian, K. , Guha, P. , Li, Y. , Bagwe, S. , Taffet, S. and Anumonwo, J.
Unique Kir2.x Properties Determine Regional and Species Differences in the Cardiac Inward Rectifier K+ Current. ,
Circ Res
 94 , 1332-9 , 2004
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 100-200 u/ml
Medium
See reference
Heart
Guinea-Pig
Guinea pig, female, 300-350g
Buxton I L, Kaiser R A, Oxhorn B C, Cheek D J.
Evidence supporting the Nucleotide Axis Hypothesis: ATP release and metabolism by coronary endothelium ,
Am J Physiol Heart Circ Physiol
 281 , H1657-66 , 2001
Cells
Coronary endothelial
Enzymes
Medium
see reference
Heart
Guinea-Pig
Guinea pig, 250-450 g
Preisig-Muller, R. , Mederos Y Schnitzler, M. , Derst, C. and Daut J.
Separation of Cardiomyocytes and Coronary Endothelial Cells for Cell-Specific RT-PCR ,
Am J Physiol
 277 , H413 , 1999
Cells
Endothelial
Enzymes
Medium
Perfusing solution
(see reference)
Heart
Guinea-Pig
Guinea-pig, male, 450-600 g
Stemmer, P. , Akera,T. , Brody, T. , Rardon, D. and Watanabe, A.
Isolation and Enrichment of Ca2+-Tolerant Myocytes for Biochemical Experiments from Guinea-Pig Heart ,
Life Sci
 44 , 1231 , 1989
Cells
Myocytes
Enzymes
Hyaluronidase : 0.02%
Medium
Krebs Henseleit bicarbonate buffer
Heart
Guinea-Pig
Guinea-pig
Ishihara, K. , Mitsuiye, T. , Noma, A. and Takano, M.
The Mg2+ Block and Intrinsic Gating Underlying Inward Rectification of the K+ Current in Guinea-Pig Cardiac Myocytes ,
J Physiol
 419 , 297 , 1989
Cells
Cardiomyocytes
Enzymes
Collagenase : 0.04%
Medium
Tyrode solution, CF
Heart
Guinea-Pig
Guinea-pig
Bridge, J. , Spitzer, K. and Ershler, P.
Relaxation of Isolated Ventricular Cardiomyocytes by a Voltage-Dependent ,
Science
 241 , 823 , 1988
Cells
Myocytes
Enzymes
Hyaluronidase : 0.10%
Medium
Bicarbonate buffer, CF
Heart
Porcine
Porcine
Butcher, J. and Nerem, R.
Porcine Aortic Valve Interstitial Cells in Three-Dimensional Culture: Comparison of Phenotype with Aortic Smooth Muscle Cells. ,
J Heart Valve Dis
 13 , 478 , 2004
Cells
Aortic smooth muscle and intersitial cells
Enzymes
Collagenase : 600 u/ml
Medium
PBS
Heart
Porcine
Porcine
Christ, M. , Gunther, A. , Heck, M. , Schmidt, B. , Falkenstein, E. and Wehling, M.
Aldosterone, Not Estradiol, Is the Physiological Agonist for Rapid Increases in cAMP in Vascular Smooth Muscle Cells ,
Circulation
 99 , 1485 , 1999
Cells
Coronary vascular smooth muscle (PCVSMCs)
Enzymes
Trypsin : 0.037%
Medium
HEPES
Heart
Porcine
Miniature swine, adult, female, 25-40 kg, familiarized with treadmill exercise
Bowles, D. , Hu, Q. , Laughlin, M. and Sturek, M.
Exercise Training Increases L-type Calcium Current Density in Coronary Smooth Muscle ,
Am J Physiol
 275 (44) , H2159 , 1998
Cells
Coronary smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Krebs bicarbonate solution
Kidney
Guinea-Pig
Guinea-pig, male, 100 g
Phillips, H.
Dissociation of Single Cells from Lung or Kidney Tissue with Elastase ,
In Vitro
 8 , 101 , 1972
Cells
Single cells
Enzymes
Trypsin : 0.25%
Medium
CF salt solution
Kidney
Porcine
Porcine, hybrid
Heussner, A. and Dietrich, D.
Primary Porcine Proximal Tubular Cells as an Alternative to Human Primary Renal Cells in Vitro: an Initial Characterization. ,
BMC Cell Biol
 14 , 55 , 2013
Cells
Kidney
Enzymes
Medium
HBSS
Liver
Guinea-Pig
Guinea-pig, Hartley, male, 250-300 g
Arinze, I. and Kawai, Y.
Adrenergic Regulation of Glycogenolysis in Isolated Guinea-Pig Hepatocytes: Evidence that B2-Receptors Mediate Catecholamine Stimulation of Glycogenolysis ,
Arch Biochem Biophys
 225 , 196 , 1983
Cells
Hepatocytes
Medium
Kreb's Ringer bicarbonate buffer
Liver
Guinea-Pig
Guinea-pig, young
Reese, J. and Byard, J.
Isolation And Culture of Adult Hepatocytes from Liver Biopsies ,
In Vitro
 17 , 935 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 90 u/ml
Medium
CF EGTA perfusate
Liver
Porcine

Porcine

Almalla, A. , Elomaa, L. , Bechtella, L. , Daneshgar, A. , Yavvari, P. and Mahfouz, Z.
Papain-Based Solubilization of Decellularized Extracellular Matrix for the Preparation of Bioactive, Thermosensitive Pregels. , Biomacromolecules Vol. 24 , 5620-5637 , 2023
Cells
Decellularized Extracellular Matrix
Enzymes
Papain : 0.05%
Medium
PBS
Liver
Porcine
Porcine, mini-pig, 13 kg
Meng, F. , Chen, Z. , Han, M. , Hu, X. , He, X. , Liu, Y. , He, W. , Huang, W. , Guo, H. and Zhou, P.
Porcine Hepatocyte Isolation and Reversible Immortalization Mediated by Retroviral Transfer and Site-Specific Recombination. ,
World J Gastroenterol
 16 , 1660 , 2010
Cells
Hepatocytes
Enzymes
Deoxyribonuclease I : see reference
Medium
Williams E
Liver
Porcine
Porcine, Yorkshire, male, 21 day
Terner, M. , Gilmore, W.J. , Lou, Y. and Squires, E.J.
The Role of CYP2A and CYP2E1 in the Metabolism of 3-Methylindole in Primary Cultured Porcine Hepatocytes. ,
Drug Metab Dispos
 34 , 848 , 2006
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Porcine
Porcine, Chinese mini pig, 6-10 kg
Li, J, Li, L. , Chao, H. , Yang, Q. , Liu, X. , Sheng, J. , Yu, H. and Huang, J
Isolation and Short Term Cultivation of Swine Hepatocytes for Bioartificial Liver Support System. ,
Hepatobiliary Pancreat Dis Int
 4 , 249 , 2005
Cells
Hepatocytes
Enzymes
Collagenase Type 4 : 125 u/ml
Medium
Williams E
Liver
Porcine
Porcine, male, 2-3 week
Raman Priya, Donkin Shawn S, Spurlock Michael E.
Regulation of hepatic glucose metabolism by leptin in pig and rat primary hepatocyte cultures ,
Am J Physiol Regul Integr Comp Physiol
 286 , R206-16 , 2004
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
DMEM
Liver
Porcine
Porcine, one week
Wang Y. , Liu H. , Guo H. , Wen H. and Liu J.
Primary Hepatocyte Culture in Collagen Gel Mixture and Collagen Sandwich ,
World J Gastroenterol
 10 , 699 , 2004
Cells
Hepatocytes
Enzymes
Medium
RPMI 1640
Liver
Porcine
Porcine, adult
Zhou X, Liu L, Kano J, Mukaiyama T. and Tokiwa T.
Isolation and Cultivation of Porcine Hepatocytes for Extracorporeal Artificial Liver Support System ,
Chin Med J
 114 , 946 , 2001
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
MEM
Liver
Porcine
Porcine, male, 6-40 kg
Gerlach, J. , Brombacher, J. , Smith, M. and Neuhaus, P.
High Yield Hepatocyte Isolation from Pig Livers for Investigation of Hybrid Liver Support Systems: Influence of Collagenase Concentration and Body Weight ,
J Surg Res
 62 (1) , 85 , 1996
Cells
Hepatocytes
Enzymes
Collagenase : 0.8%
Medium
PBS
Lung
Guinea-Pig
Guinea-pig, male, 250-300 g
Sikpi, M. , Nair, C. , Johns, A. and Das, S.
Metabolic and Ultrastructural Characterizationof Guinea-Pig Alveolar Type II Cells Isolated by Centrifugal Elutriation ,
Biochim Biophys Acta
 877 , 20 , 1986
Cells
Alveolar type II
Enzymes
Elastase : 40 u/ml
Medium
PBS
(see reference)
Lung
Guinea-Pig
Guinea-pig, male, 100 g
Phillips, H.
Dissociation of Single Cells from Lung or Kidney Tissue with Elastase ,
In Vitro
 8 , 101 , 1972
Cells
Single cells
Enzymes
Trypsin : 0.25%
Medium
CF salt solution
Lung
Porcine
Swine fetuses (70 day), adult Amer Dutch 250 day
(also human fetuses, 80 day)
Hinz, R. and Syverton, J.
Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase ,
Proc Soc Exp Biol Med
 101 , 19 , 1959
Cells
Lung
Enzymes
Collagenase : 0.01%
Medium
HBSS
Mammary
Guinea-Pig
Guinea-pig, pregnant, 4-10 day
Turba, F. and Hilpert, N.
Secretion and Resorption of Proteins by Isolated Mammary Gland Cells. German ,
Biochem Z
 334 , 501 , 1961
Cells
Mammary gland
Enzymes
Medium
Dulbecco phosphate
Miscellaneous
Porcine
Porcine, 6 month
Kean, T. and Dennis, J.
Synoviocyte Derived-Extracellular Matrix Enhances Human Articular Chondrocyte Proliferation and Maintains Re-Differentiation Capacity at Both Low and Atmospheric Oxygen Tensions. ,
PLoS ONE
 10 , e0129961 , 2015
Cells
Synoviocytes
Enzymes
Hyaluronidase : 660 u/ml
Trypsin : 0.25%
Collagenase Type 2 : 583 u/ml
Medium
DMEM
Miscellaneous
Porcine
Porcine, <10 week
Potier, E. and Ito. K.
Can Notochordal Cells Promote Bone Marrow Stromal Cell Potential for Nucleus Pulposus Enrichment? A Simplified in Vitro System. ,
Tissue Eng Part A
 20 , 3241-51 , 2014
Cells
Nucleus pulposus
Enzymes
Collagenase : 0.05%
Pronase : 0.3%
Medium
DMEM
Miscellaneous
Porcine
Porcine, various ages
Cho, H. , Park, S. , Lee, S. , Kang, M. , Hasty, K. and Kim, S.
Snapshot of Degenerative Aging of Porcine Intervertebral Disc: A Model to Unravel the Molecular Mechanisms. ,
Exp Mol Med
 43 , 334-40 , 2011
Cells
Nucleous pulposus and AF
Enzymes
Pronase : 0.05%
Collagenase : 0.2%
Medium
Modified F-12K
Muscle
Guinea-Pig
Guinea-pig
Shieh CC, Feng J, Buckner SA, Brioni JD, Coghlan MJ, Sullivan JP, Gopalakrishnan M.
Functional implication of spare ATP-sensitive K(+) channels in bladder smooth muscle cells ,
J Pharmacol Exp Ther
 296 , 669-75 , 2001
Cells
Bladder smooth muscle
Enzymes
Collagenase Type 2 : 0.1-0.2%
Medium
Krebs-Ringer bicarbonate
Muscle
Guinea-Pig
Guinea pig, 2-4 wk old, male, female
Firth, T. , Mawe, G. and Nelson, M.
Pharmacology and Modulation of KATP Channels by Protein Kinase C and Phosphatates in Gallbladder Smooth Muscle ,
Am J Physiol Cell Physiol
 278 , C1031 , 2000
Cells
Smooth muscle
Gallbladder
Enzymes
Papain : 0.1%
Medium
Krebs solution
Muscle
Guinea-Pig
Guinea-pig, 200-380 g
Schnitzler, M. , Derst, C. , Daut, J. and Preisig-Muller, R.
ATP-Sensitive Potassium Channels in Capillaries Isolated From Guinea-Pig Heart ,
J Physiol
 525 (2) , 307 , 2000
Cells
Capillaries
Myocytes
Enzymes
Medium
CF solution
Muscle
Guinea-Pig
Guinea-pig, adult, 250-350 g
Jennings, L. , Xu, Q. , Firth, T. , Nelson, M. and Mawe, G.
Cholesterol Inhibits Spontaneous Action Potentials and Calcium Currents in Guinea Pig Gallbladder Smooth Muscle ,
Am J Physiol
 277 , G1017 , 1999
Cells
Smooth muscle
Gallbladder
Enzymes
Papain : 0.1%
Medium
NaCl, sodium glutamate, MgCl, KCl, glucose, Kreb's, and HEPES
Muscle
Guinea-Pig
Guinea-pig, Dunkin-Hartley, female
Ryder, K. , Bryant, S. and Hart, G.
Membrane Current Changes in Left Ventricular Myocytes Isolated From Guinea-Pigs After Abdominal Aortic Coarctation ,
Cardiovasc Res
 27 , 1278 , 1993
Cells
Myocytes
Medium
DMEM
Muscle
Guinea-Pig
Guinea-pig
(also rat, rabbit)
Hu, S. and Kim, H.
Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid ,
FASEB J
 6 , A383 , 1992
Cells
Smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Potassium buffer solution
Muscle
Guinea-Pig
Guinea-pig, 200-300 g
Ohya, Y. and Sperelakis, N.
ATP Regulation of the Slow Calcium Channels in Vascular Smooth Muscle Cells ,
Circ Res
 64 , 145 , 1989
Cells
Smooth muscle
Mesenteric artery
Enzymes
Collagenase : 0.3%
Medium
CF solution

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.