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Tissue References Search Tool

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Kidney
Human
Human
Trifillis, A. and Kahng, M.
Characterization of an In Vitro System of Human Renal Papillary Collecting Duct Cells ,
In Vitro Cell Dev Biol
 26 , 441 , 1990
Cells
Papillary duct
Enzymes
Collagenase : 400 u/ml
Medium
Eagle's MEM-HEPES buffer w/L-glutamine
Kidney
Human
Human
Heieren, M. , van der Woude, F. and Balfour Jr., H.
Cytomegalovirus Replicates Efficiently in Human Kidney Mesangial Cells ,
Proc Natl Acad Sci U S A
 85 , 1642 , 1988
Cells
Mesangial
Enzymes
Trypsin : 0.25%
Medium
DMEM/Ham's F-12
Kidney
Human
Human, adult
Yang, A. , Gould-Kostka, J. and Oberley, T.
In Vitro Growth and Differentiation of Human Kidney Tubular Cells on a Basement Membrane Substrate ,
In Vitro Cell Dev Biol
 23 (1) , 34 , 1987
Cells
Tubular
Enzymes
Collagenase : 250 u/ml
Medium
PBS
Kidney
Human
Human, adult, 14-66 years
Trifillis, A. , Regec, A. and Trump, B.
Isolation, Culture, and Charaterization of Human Renal Tubular Cells ,
J Urol
 133 , 324 , 1985
Cells
Tubular
Enzymes
Collagenase : 100 u/ml
Medium
Joklik's MEM
Kidney
Human
Human
Hemstreet, G. , Enoch, P. and Pretlow, T.
Tissue Disaggregation of Human Renal Cell Carcinoma with Further Isopyknic and Isokinetic Gradient Purification ,
Cancer Res
 40 , 1043 , 1980
Cells
Malignant
Stromal
Enzymes
Papain : 0.009%
Medium
Sacks solution
Kidney
Human
Human, 24 newborn and stillborn
(also rabbit)
De Oca, H. and Malinin, T.
Dispersion and Cultivation of Renal Cells After Short-Term Storage of Kidneys ,
J Clin Microbiol
 2 , 243 , 1975
Cells
Renal
Enzymes
Trypsin : 0.25%
Medium
See reference
Kidney
Mouse

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.
Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
 13 , e4679 , 2023
Cells
T cells
Enzymes
Medium
RPMI
Kidney
Mouse

Mouse

Li, Z. , Zimmerman, K. , Cherakara, S. , Chumley, P. , Collawn, J. , Wang, J. , Haycraft, C. , Song, C. , Chacana, T. , Andersen, R. , Croyle, M. , Aloria, E. , Hombal, R. , Thomas, I. , Chweih, H. , Simanyi, K. , George, J. , Parant, J. , Mrug, M. and Yoder, B.
A Kidney Resident Macrophage Subset is a Candidate Biomarker for Renal Cystic Disease in Preclinical Models. , Dis Model Mech Vol. 16 , 2023
Cells
Macrophages
Enzymes
Medium
RPMI 1640
Kidney
Mouse
Mouse
Yuan, Y. , Li, L. , Zhu, L. , Liu, F. , Tang, X. , Liao, G. , Liu, J. , Cheng, J. , Chen, Y. and Lu, Y.
Mesenchymal Stem Cells Elicit Macrophages into M2 Phenotype Via Improving Transcription Factor EB-Mediated Autophagy to Alleviate Diabetic Nephropathy. ,
Stem Cells
 38 , 639-652 , 2020
Cells
Mactrophages
Enzymes
Medium
DMEM
Kidney
Mouse
Mouse
Adam, M. , Potter, A. and Potter, S.
Psychrophilic Proteases Dramatically Reduce Single-Cell RNA-Seq Artifacts: A Molecular Atlas of Kidney Development. ,
Development
 144 , 3625-3632 , 2017
Cells
Kidney
Enzymes
Deoxyribonuclease I : 125 u/ml
Protease, Bacillus Licheniformis : 1%
Medium
DPBS
Kidney
Mouse
Mouse
DiRocco, D. , Kobayashi, A. , Taketo, M. , McMahon, A. and Humphreys, B.
Wnt4/β-catenin Signaling in Medullary Kidney Myofibroblasts. ,
J Am Soc Nephrol
 24 , 1399 , 2013
Cells
Collecting duct epithelial
Enzymes
Medium
DMEM
Kidney
Mouse
Mouse
Sedrakyan, S. , Da Sacco, S. , Milanesi, A. , Shiri, L. , Petrosyan, A. , Varimezova, R. , Warburton, D. , Lemley, K. , De Filippo, R. and Perin, L.
Injection of Amniotic Fluid Stem Cells Delays Progression of Renal Fibrosis. ,
J Am Soc Nephrol
 23 , 661-73 , 2012
Cells
Glomeruli
Enzymes
Medium
DMEM
Kidney
Mouse
Mouse
Kabgani, N. , Grigoleit, T. , Schulte, K. , Sechi, A. , Sauer-Lehnen, S. , Tag, C. , Boor, P. , Kuppe, C. , Warsow, G. , Schordan, S. , Mostertz, J. , Chilukoti, R. , Homuth, G. , Endlich, N. , Tacke, F. and Weiskirchen, R.
Primary Cultures of Glomerular Parietal Epithelial Cells or Podocytes with Proven Origin. ,
PLoS ONE
 7 , e34907 , 2012
Cells
Glomeruli
Enzymes
Medium
RPMI 1640
Kidney
Mouse
Mouse, 10-14 week
Park, J. , Pasupulati, R. , Feldkamp, T. , Roeser, N. and Weinberg, J.
Cyclophilin D and the Mitochondrial Permeability Transition in Kidney Proximal Tubules after Hypoxic and Ischemic Injury. ,
Am J Physiol/Renal
 301 , F134 , 2011
Cells
Proximal tubule
Enzymes
Medium
See reference
Kidney
Mouse
Mouse, embryonic
Brown, A. , Blank, U. , Adams, D. , Karolak, M. , Fetting, J. , Hill, B. and Oxburgh, L.
Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney. ,
J Vis Exp
 50 , e2555 , 2011
Cells
Kidney
Enzymes
Medium
HBSS
Kidney
Mouse
Mouse, adult
Huang, Y. , Johnston, P. , Zhang, B. , Zakari, A. , Chowdhry, T. , Smith, R. , Marban, E. , Rabb, H. and Womer, K.
Kidney-Derived Stromal Cells Modulate Dendritic and T Cell Responses. ,
J Am Soc Nephrol
 20 , 831-41 , 2009
Cells
Kidney sphere-derived
Enzymes
Medium
Iscove's Modified Dulbecco's
Kidney
Mouse
Mouse, male, 8-16 week
Breggia, A. and Himmelfarb, J.
Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress. ,
Oxid Med Cell Longev
 1 , 33 , 2008
Cells
Renal tubular epithelial
Enzymes
Collagenase : 200 u/ml
Soybean Trypsin Inhibitor : see reference
Medium
HBSS
Kidney
Mouse
Mouse
Wright, J. , Morales, M. , Sousa-Menzes, J. , Ornellas, D. , Sipes, J. , Cui, Y. , Cui, I. , Hulamm, P. , Cebotaru, V. , Cebotaru, L. , Guggino, W. and Guggino, S.
Transcriptional Adaptation to Clcn5 Knockout in Proximal Tubules of Mouse Kidney. ,
Physiol Genomics
 33 , 341 , 2008
Cells
Proximal tubule
Enzymes
Medium
see reference
Kidney
Mouse
Mouse, 4 week
Kondo, S. , Scheef, E. , Sheibani, N. and Sorenson, C.
PECAM-1 Isoform-Specific Regulation of Kidney Endothelial Cell Migration and Capillary Morphogenesis. ,
Am J Physiol Cell Physiol
 292 , C2070 , 2007
Cells
Endothelial kidney
Enzymes
Medium
DMEM
Kidney
Mouse
Mouse, adult
Syal Ashu, Schiavi Susan, Chakravarty Sumana, Dwarakanath Vangipuram, Quigley Raymond, Baum Michel.
Fibroblast growth factor-23 increases mouse PGE2 production in vivo and in vitro ,
Am J Physiol/Renal
 290 , F450-5 , 2006
Cells
Cortex, proximal tubule
Enzymes
Collagenase : 0.15%
Medium
DMEM
Kidney
Mouse
Mouse
Plotkin, M. and Goligorsky, M.
Mesenchymal Cells from Adult Kidney Support Angiogenesis and Differentiate into Multiple Interstitial Cell Types including Erythropoietin-Producing Fibroblasts. ,
Am J Physiol Renal Physiol
 291 , F902-12 , 2006
Cells
Kidney mesenchymal
Enzymes
Collagenase : 250 u/ml
Neutral Protease : 33.3 u/ml
Medium
DMEM/F12
Kidney
Mouse
Mouse, 10-12 week
Dekel, B. , Zangi, L. , Shezen, E. , Reich-Zeliger, S. , Eventov-Friedman, S. , Katchman, H. , Jacob-Hirsch, J. , Amariglio, N. , Rechavi, G. , Margalit, R. and Reisner, Y.
Isolation and Characterization of Nontubular sca-1+lin- Multipotent Stem/Progenitor Cells from Adult Mouse Kidney. ,
J Am Soc Nephrol
 17 , 3300 , 2006
Cells
Kidney
Enzymes
Medium
DMEM
Kidney
Mouse
Mouse, male, 2 month
Sindic, A. , Velic, A. , Basoglu, C. , Hirsch, J. , Edemir, B. , Kuhn, M. and Schlatter, E.
Uroguanylin and Guanylin Regulate Transport of Mouse Cortical Collecting Duct Independent of Guanylate Cyclase C. ,
Kidney Int
 68 , 1008 , 2005
Cells
Cortical collecting duct
Enzymes
Collagenase : 54-178 u/ml
Protease : see reference
Medium
MEM
Kidney
Mouse
Mouse, male, 12-16 week
Cunningham, R. , Xiaofei, E. , Steplock, D. , Shenolikar, S. and Weinman, E.
Defective PTH Regulation of Sodium-Dependent Phosphate Transport in NHERF-1-/- Renal Proximal Tubule Cells and Wild-Type Cells Adapted to Low-Phosphate Media. ,
Am J Physiol Renal Physiol
 289 , F933 , 2005
Cells
Renal proximal tubule cells
Enzymes
Medium
DMEM/F-12
Kidney
Mouse
Mouse, 6 week
Radeke HH, Janssen-Graalfs I, Sowa EN, Chouchakova N, Skokowa J, Loscher F, Schmidt RE, Heeringa P, Gessner JE.
Opposite regulation of type II and III receptors for immunoglobulin G in mouse glomerular mesangial cells and in the induction of anti-glomerular basement membrane (GBM) nephritis ,
J Biol Chem
 277(30) , 27535 , 2002
Cells
Glomerular mesangial cells
Enzymes
Medium
RPMI 1640
Kidney
Mouse
Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
 10 , 1569-79 , 1999
Cells
Fibroblasts, mesangial, smooth muscle
Enzymes
Trypsin : 0.25%
Collagenase : see reference
Medium
DMEM
Kidney
Mouse
Mouse
Taub, M.
Renal Tubule Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 2 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley & Sons, Ltd. , 14B:6.1 , 1995
Cells
Epithelial
Enzymes
Medium
DMEM /F-12
Kidney
Mouse
Mouse, naive SJL (H-2S)
Haverty, T. , Kelly, C. , Hines, W. , Amenta, P. , Watanabe, M. , Harper, R. , Kefalides, N. and Neilson, E.
Characterization of a Renal Tubular Epithelial Cell Line Which Secretes the Autologous Target Antigen of Autoimmune Experimental Interstitial Nephritis ,
J Cell Biol
 107 , 1359 , 1988
Cells
Proximal tubular epithelial
Enzymes
Deoxyribonuclease I : 15 µg/ml
Medium
RPMI 1640
Liver
Human

Human

Kucykowicz, S. , Amin, O. , Burton, A. , Swadling, L. , Schmidt, N. , Zakeri, N. , Davies, J. , Aidoo-Micah, G. , Stegmann, K. , Easom, N. , Jeffery-Smith, A. , Maini, M. and Pallett, L.
Isolation of Human Intrahepatic Leukocytes for Phenotypic and Functional Characterization by Flow Cytometry. ,
Star Protocols
 3 , 101356 , 2022
Cells
Intrahepatic leukocytes
Enzymes
Medium
RPMI-1640
Liver
Human
Human
Reuschel, E, Jilg, W. , Seelbach-Goebel, B. and Demi, L.
Comparative Purification and Characterization of Hepatitis B Virus-like Particles Produced by Recombinant Vaccinia Viruses in Human Hepatoma Cells and Human Primary Hepatocytes ,
PLoS ONE
 , 2019
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Human
Human
Pfeiffer, E. , Kegel, V. , Zeilinger, K. , Hengstler, J. , Nussler, A. , Seehofer, D. and Damm, G.
Featured Article: Isolation, Characterization, and Cultivation of Human Hepatocytes and Non-Parenchymal Liver Cells. ,
Exp Biol Med (Maywood)
 240 , 645-56 , 2015
Cells
Hepatocytes and non-parenchymal cells
Enzymes
Medium
HBSS
Liver
Human
Human
Werner, M. , Driftmann, S. , Kleinehr, K. , Kaiser, G. , Mathe, Z. , Treckmann, J. , Paul, A. , Skibbe, K. , Timm, J. , Canbay, A. , Gerken, G. , Schlaak, J. and Broering, R.
All-In-One: Advanced Preparation of Human Parenchymal and Non-Parenchymal Liver Cells. ,
PLoS ONE
 10 , e0138655 , 2015
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Human
Human
Lee, S. , Schelcher, C. , Demmel, M. , Hauner, M. and Thasler, W.
Isolation of Human Hepatocytes by a Two-Step Collagenase Perfusion Procedure. ,
J Vis Exp
 , 2013
Cells
Hepatocytes
Enzymes
Collagenase : 0.1-0.15%
Medium
HEPES
Liver
Human
Human, fetal
Vatakis, D. , Bristol, G. , Kim, S. , Levin, B. , Liu, W. , Radu, C. , Kitchen, S. and Zack, J.
Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model. ,
J Vis Exp
 70 , e4181 , 2012
Cells
Liver hematopoietic
Enzymes
Medium
RPMI
Liver
Human
Human
Torok, E. , Lutgehetmann, M. , Bierwolf, J. , Melbeck, S. , Dullmann, J. , Nashan, B. , Ma, P. and Pollok, J.
Primary Human Hepatocytes on Biodegradable Poly(l-lactic acid) Matrices: A Promising Model for Improving Transplantation Efficiency with Tissue Engineering. ,
Liver Transpl
 17 , 104 , 2011
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Human
Human
Wauthier, E. , Schmelzer, E. , Turner, W. , Zhang, L. , LeCluyse, E. , Ruiz, J. , Turner, R. , Furth, M. , Kubota, H. , Lozoya, O. , Barbier, C. , McClelland, R. , Yao, H. , Moss, N. , Bruce, A. , Ludlow, J. and Reid, L.
Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance. ,
Methods Cell Biol
 86 , 137 , 2008
Cells
Hepatic stem cells and heptoblasts
Enzymes
Collagenase Type 4 : 0.014-0.06%
Medium
various

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.