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Tissue References Search Tool

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Skin
Human
Human
Babina Magda, Guhl Sven, Starke Andrae, Kirchhof Loreen, Zuberbier Torsten, Henz Beate M.
Comparative cytokine profile of human skin mast cells from two compartments--strong resemblance with monocytes at baseline but induction of IL-5 by IL-4 priming ,
J Leukoc Biol
 75 , 244-52 , 2004
Cells
Mast cells
Enzymes
Medium
RPMI
Skin
Human
Human
Tuan Tai-Lan, Wu Huayang, Huang Eunice Y, Chong Sheree S N, Laug Walter, Messadi Diana, Kelly Paul, Le Anh.
Increased plasminogen activator inhibitor-1 in keloid fibroblasts may account for their elevated collagen accumulation in fibrin gel cultures ,
Am J Pathol
 162 , 1579-89 , 2003
Cells
Fibroblasts
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Human
Human
Supp Dorothy M, Wilson-Landy Kaila, Boyce Steven T.
Human dermal microvascular endothelial cells form vascular analogs in cultured skin substitutes after grafting to athymic mice ,
FASEB J
 16 , 797-804 , 2002
Cells
Keratinocytes, fibroblasts, endothelial
Enzymes
Neutral Protease : see reference
Collagenase : see reference
Medium
see reference
Skin
Human
Human, adult
Baudoux, B. , Castanares-Zapatero, D. , Leclercq-Smekens, M. , Berna, N. and Poumay, Y.
The Tetraspanin CD9 Associates with the Integrin a6B4 in Cultured Human Epidermal Keratinocytes and is Involved in Cell Motility ,
Eur J Cell Biol
 79 , 41 , 2000
Cells
Keratinocytes
Enzymes
Medium
PBS
Skin
Human
Human
Hybbinette S, Bostrom M, Lindberg K.
Enzymatic dissociation of keratinocytes from human skin biopsies for in vitro cell propagation ,
Exp Dermatol
 8 , 30-8 , 1999
Cells
Keratinocytes
Enzymes
Trypsin : 0.17%
Thermolysin : 0.05%
Medium
see reference
Skin
Human
Human
Grutzkau A, Kruger-Krasagakes S, Baumeister H, Schwarz C, Kogel H, Welker P, Lippert U, Henz BM, Moller A.
Synthesis, storage, and release of vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) by human mast cells: implications for the biological significance of VEGF206 ,
Mol Biol Cell
 9 , 875-84 , 1998
Cells
Human skin mast cells
Enzymes
Collagenase Type 1 : see reference
Medium
PBS
Skin
Human
Human
Reece J C, Handley A J, Anstee E J, Morrison W A, Crowe S M, Cameron P U.
HIV-1 selection by epidermal dendritic cells during transmission across human skin ,
J Exp Med
 187 , 1623-31 , 1998
Cells
Epidermal
Enzymes
Medium
PBS
Skin
Human
Human
Judd, D. , Battista, P. and Behm, D.
Culture of Human Keratinocytes in Defined Serum-Free Medium ,
Focus
 19 (1) , 2 , 1997
Cells
Keratinocytes
Enzymes
Trypsin : 0.05%
Medium
Dulbecco's PBS
Skin
Human
Human
Harley, C. and Sherwood, S.
, Methods in Molecular Biology, Basic Cell Culture Protocols, 2nd ed. Vol. 75 , Pollard, J., and Walker, J. , Humana Press , 23 , 1997
Cells
Epidermis plus dermis
Abdomen or inner forearm
Enzymes
Trypsin : 0.125%
Medium
DMEM, M199
Skin
Human
Human
Regnier, M.
Culture of Human Karatinocytes , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons Ltd , 11B:4.1 , 1995
Cells
Keratinocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Human
Human, adult, female, breast skin
Hirel, B. , Chesne, C. , Pailgheret, J. and Guillouzo, A.
Expression of Differentiation Markers in Human Adult Keratinocytes Cultured in Submerged Conditions ,
In Vitro Cell Dev Biol
 30A , 372 , 1994
Cells
Keratinocytes
Enzymes
Trypsin : 0.25%
Medium
PBS
Skin
Human
Human, neonatal foreskin
Hansbrough, J. , Cooper, M. , Cohen, R. , Spielvogel, R. , Greenleaf, G. , Bartel, R. and Naughton, G.
Evaluation of a Biodegradable Matrix Containing Cultured Human Fibroblasts as a Dermal Replacement Beneath Meshed Skin Grafts on Athymic Mice ,
Surgery
 111 (1) , 438 , 1992
Cells
Fibroblasts
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Human
Human, 18-50 years
Bell, C. and Quinton, P.
Effects of Media Buffer Systems on Growth and Electrophysiologic Characteristics of Cultured Sweat Duct Cells ,
In Vitro Cell Dev Biol
 27 , 47 , 1991
Cells
Sweat duct
Enzymes
Collagenase : 0.03%
Medium
MEM
Skin
Human
Human
Limat, A. , Hunziker, T. , Boillat, C. , Noser, F. and Wiesmann, U.
Postmitotic Human Dermal Fibroblasts Preserve Intact Feeder Properties for Epithelial Cell Growth After Long-Term Cryopreservation ,
In Vitro Cell Dev Biol
 26 , 709 , 1990
Cells
Fibroblasts
Enzymes
Trypsin : 0.25%
Medium
CMF solution
Skin
Human
Human
Krouse, M. , Hagiqara, G. , Chen, J. , Lewiston, N. and Wine, J.
Ion Channels in Normal Human and Cystic Fibrosis Sweat Gland Cells ,
Am J Physiol
 257 , C129 , 1989
Cells
Sweat gland
Enzymes
Medium
(see reference)
Skin
Human
Human, ages 16-30
Pedersen, P.
Human Sweat Duct Cells in Primary Culture. Basic Bioelectric Properties of Cultures Derived From Normals and Patients with Cystic Fibrosis ,
In Vitro Cell Dev Biol
 25 (4) , 342 , 1989
Cells
Human sweat duct
Enzymes
Medium
RPMI 1640
(see reference)
Skin
Human
Human
Peacocke, M. , Yaar, M. , Mansur, C. , Chao, M. and Gilchrest, B.
Induction of Nerve Growth Factor Receptors on Cultured Human Melanocytes ,
Proc Natl Acad Sci U S A
 85 , 5282 , 1988
Cells
Melanocytes, skin/ foreskin
Enzymes
Trypsin : 0.25%
Medium
PBS
Skin
Human
Human
Lee, C. , Carpenter, F. , Coaker, T. and Kealey, T.
The Primary Culture of Epithelia From the Secretory Coil and Collecting Duct of Normal Human and Cystic Fibrotic Eccrine Sweat Glands ,
J Cell Sci
 83 , 103 , 1986
Cells
Human sweat glands
Enzymes
Collagenase : 0.2%
Medium
HBSS
Skin
Human
Human
Dover, R. and Potten, C.
Cell Cycle Kinetics of Cultured Human Epidermal Keratinocytes ,
J Invest Dermatol
 80 , 423 , 1983
Cells
Keratinocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Human
Human, 18-30 years, male, female
McCoy, B. , Galdun, J. and Cohen, I.
Effects of Density and Cellular Aging On Collagen Synthesis and Growth Kinetics in Keloid and Normal Skin Fibroblasts ,
In Vitro
 18 (1) , 79 , 1982
Cells
Fibroblasts
Enzymes
Trypsin : 0.1%
Medium
HBSS
Skin
Human
Human, adult
Alitalo K, Kuismanen E, Myllyla R, Kiistala U, Asko-Seljavaara S, Vaheri A.
Extracellular matrix proteins of human epidermal keratinocytes and feeder 3T3 cells ,
J Cell Biol
 94 , 497-505 , 1982
Cells
Epidermal keratinocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.2%
Medium
Eagle's MEM
Skin
Human
Human, fetal
Davies, P. and Kerr, C.
Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts ,
Biochim Biophys Acta
 712 , 26 , 1982
Cells
Smooth muscle, fibroblasts
Enzymes
Trypsin : 0.055%
Medium
DMEM
Skin
Human
Human, newborn
Liu, S. , Eaton, M. and Karasek, M.
Growth Characteristics of Human Epidermal Keratinocytes from Newborn Foreskin in Primary and Serial Cultures ,
In Vitro
 15 (10) , 813 , 1979
Cells
Keratinocytes
Enzymes
Trypsin : 0.3%
Medium
DMEM
Skin
Human
Human
Rheinwald, J. and Green, H.
Serial Cultivation of Strains of Human Epidermal Kerotinocytes: The Formation of Keratinizing Colonies from Single Cells ,
Cell
 6 , 331 , 1975
Cells
Keratinocytes
Enzymes
Trypsin : 0.25%
Medium
(see reference)
Skin
Porcine
Porcine, 3-4 month
Ando, W. , Kutcher, J. , Krawetz, R. , Sen, A. , Nakamura, N. , Frank, C. and Hart, D.
Clonal Analysis of Synovial Fluid Stem Cells to Characterize and Identify Stable Mesenchymal Stromal Cell/Mesenchymal Progenitor Cell Phenotypes in a Porcine Model: A Cell Source with Enhanced Commitment to the Chondrogenic Lineage. ,
Cytotherapy
 16 , 776 , 2014
Cells
Synovial membrane and skin stem
Enzymes
Collagenase : 0.2%
Medium
DMEM
Skin
Porcine
Porcine, 2-6 month
Regauer S, Compton C.
Cultured porcine epithelial grafts: an improved method ,
J Invest Dermatol
 94 , 230-4 , 1990
Cells
Keratinocytes
Enzymes
Medium
Dulbecco-Vogt MEM
Stem
Human
Human
Alstrup, T. , Eijken, M. , Bohn, A. , Moller, B. and Damsgaard, T.
Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat. ,
Curr Protoc Stem Cell Biol
 48 , e68 , 2019
Cells
Adipose derived stem
Enzymes
Collagenase Type 4 : 150-300 u/ml
Medium
RPMI-1640
Stem
Human
Human
Yam, G. , Teo, E. , Setiawan, M. , Lovatt, M. , Yusoff, N. , Fuest, M. , Goh, B. and Mehta, J.
Postnatal Periodontal Ligament as a Novel Adult Stem Cell Source for Regenerative Corneal Cell Therapy. ,
J Cell Mol Med
 , 2018
Cells
Postnatal periodontal ligament
Enzymes
Medium
DMEM/F12
Stem
Human
Human
Matsumura, E. , Tsuji, K. , Komori, K. , Koga, H. , Sekiya, I. and Muneta, T.
Pretreatment with IL-1β Enhances Proliferation and Chondrogenic Potential of Synovium-Derived Mesenchymal Stem Cells. ,
Cytotherapy
 19 , 181-193 , 2017
Cells
Synovial mesenchymal stem
Enzymes
Collagenase : 0.3%
Medium
MEM
Stem
Human
Human
Clark, K. , Fierro, F. , Ko, E. , Walker, N. , Arzi, B. , Tepper, C. , Dahlenburg, H. , Cicchetto, A. , Koi, A. , Marsh, L. , Murphy, W. , Fazel, N. and Borjesson, D.
Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome. ,
Stem Cell Res Ther
 8 , 69 , 2017
Cells
Adipose derived mesenchymal stem
Enzymes
Medium
PBS
Stem
Human
Human
de Witte, S. , Lambert, E. , Merino, A. , Strini, T. , Douben, H. , O'Flynn, L. , Elliman, S. , de Klein, A. , Newsome, P. , Baan, C. and Hoogduijn, M.
Aging of Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stromal Cells During Expansion. ,
Cytotherapy
 19 , 798-807 , 2017
Cells
Umbilical cord mesenchymal stromal
Enzymes
Medium
MEM
Stem
Human
Human
Oppliger, B. , Joerger-Messerli, M. , Simillion, C. , Mueller, M. , Surbek, D. and Schoeberlein, A.
Mesenchymal Stromal Cells from Umbilical Cord Wharton's Jelly Trigger Oligodendroglial Differentiation in Neural Progenitor Cells Through Cell-to-Cell Contact. ,
Cytotherapy
 19 , 829-838 , 2017
Cells
Wharton jelly mesenchymal stromal
Enzymes
Collagenase Type 2 : 270 u/ml
Medium
DMEM/F12
Stem
Human
Human
Rigamonti, A. , Repetti, G. , Sun, C. , Price, F. , Reny, D. , Rapino, F. , Weisinger, K. , Benkler, C. , Peterson, Q. , Davidow, L. , Hansson, E. and Rubin, L.
Large-Scale Production of Mature Neurons from Human Pluripotent Stem Cells in a Three-Dimensional Suspension Culture System. ,
Stem Cell Reports
 6 , 993-1008 , 2016
Cells
Motor neurons
Enzymes
Papain : 20 u/ml
Medium
EBSS
Stem
Human
Human
Randelli, P. , Menon, A. , Ragone, V. , Creo P. , Alfieri, M. , Perucca, O. , Banfi, G. , Cabitza, P. , Tettamanti, G. and Anastasia, L.
Effects of the Pulsed Electromagnetic Field PST on Human Tendon Stem Cells: a Controlled Laboratory Study. ,
BMC Complement Altern Med
 16 , 293 , 2016
Cells
Tendon stem
Enzymes
Medium
MEM
Stem
Human
Human
Cheng, N. , Hsieh, T. , Lai, H. and Young, T.
High Glucose-Induced Reactive Oxygen Species Generation Promotes Stemness in Human Adipose-Derived Stem Cells ,
Cytotherapy
 18 , 371-83 , 2016
Cells
Adipose stem
Enzymes
Medium
DMEM/F12
Stem
Human
Human
Lim, J. , Razi, Z. , Law, J. , Nawi, A. , Idrus, R. and Ng, M.
MSCs can be Differentially Isolated From Maternal, Middle and Fetal Segments of the Human Umbilical Cord. ,
Cytotherapy
 18 , 1493-1502 , 2016
Cells
Wharton jelly mesenchymal stromal
Enzymes
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.