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Miscellaneous
Human

Human, 18-30 years

Pardo-Rodríguez, B. , Baraibar, A. , Manero-Roig, I. , Luzuriaga, J. , Salvador-Moya, J. , Polo, Y. , Basanta-Torres, R. , Unda, F. , Mato, S. , Ibarretxe, G. and Pineda, J.
Functional Differentiation of Human Dental Pulp Stem Cells into Neuron-like Cells Exhibiting Electrophysiological Activity. ,
Stem Cell Res Ther
 16 , 10 , 2025
Cells
Dental pulp stem cells
Enzymes
Medium
DMEM
Miscellaneous
Human

Human

Xue, Y. , Seiler, M. , Tang, W. , Wang, J. , Delgado, J. , McLelland, B. , Nistor, G. , Keirstead, H. and Browne, A.
Retinal Organoids On-a-Chip: a Micro-Millifluidic Bioreactor for Long-term Organoid Maintenance. ,
Lab Chip
 21 , 3361-3377 , 2021
Cells
Stem cell-derived retinal organoids
Enzymes
PDS kit : see reference
Medium
EBSS
Miscellaneous
Human
Human
Foster, D. , Marshall, C. , Gulati, G. , Chinta, M. , Nguyen, A. , Salhotra, A. , Jones, R. , Burcham, A. , Lerbs, T. , Cui, L. , King, M. , Titan, A. , Ransom, R. , Manjunath, A. , Hu, M. and Blackshear, C.
Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation. ,
Nat Commun
 11 , 4061 , 2020
Cells
Adhesion fibroblasts
Enzymes
Medium
Medium 199
Miscellaneous
Human

Human

Yoon, S. , Elahi, L. , Pașca, A. , Marton, R. , Gordon, A. , Revah, O. , Miura, Y. , Walczak, E. , Holdgate, G. , Fan, H. , Huguenard, J. , Geschwind, D. and Pașca, S.
Reliability of Human Cortical Organoid Generation. ,
Nat Methods
 16 , 75-78 , 2019
Cells
Cortical spheroids
Enzymes
Papain : 40 u/ml
Medium
EBSS
Miscellaneous
Human

Human

Velasco, S. , Kedaigle, A. , Simmons, S. , Nash, A. , Rocha, M. , Quadrato, G. , Paulsen, B. , Nguyen, L. , Adiconis, X. , Regev, A. , Levin, J. and Arlotta, P.
Individual Brain Organoids Reproducibly Form Cell Diversity of the Human Cerebral Cortex. ,
Nature
 570 , 523-527 , 2019
Cells
Brain organoids
Enzymes
PDS kit : see reference
Medium
PBS
Miscellaneous
Human
Human
Sun, Y. , Lv, M. , Zhou, L. , Tam, V. , Lv, F. , Chan, D. , Wang, H. , Zheng, Z. , Cheung, K. and Leung, V.
Enrichment of Committed Human Nucleus Pulposus Cells Expressing Chondroitin Sulfate Proteoglycans under Alginate Encapsulation. ,
Osteoarthritis Cartilage
 23 , 1194-203 , 2015
Cells
Nucleus pulposus
Enzymes
Pronase : 0.25%
Collagenase Type 2 : 600 u/ml
Medium
DMEM
Miscellaneous
Human
Human, 3-7 year
Salmon, B. , Bardet, C, Khaddam, M. Naji, J. , Coyac, B. , Baroukh, B. , Letourneur, F. , Lesieur, J. , Decup, F. , Le Denmat, D. , Nicoletti, A. , Poliard, A, Rowe, P. , Huet, E. , Vital, S. and Linglart, A.
MEPE-Derived ASARM Peptide Inhibits Odontogenic Differentiation of Dental Pulp Stem Cells and Impairs Mineralization in Tooth Models of X-linked Hypophosphatemia. ,
PLoS ONE
 8 , e56749 , 2013
Cells
Dental pulp stem cells
Enzymes
Medium
DMEM
Miscellaneous
Human
Human
Bonnamain, V. , Thinard, R. , Sergent-Tanguy, S. , Huet, P. , Bienvenu, G. , Naveilhan, P. , Farges, J. and Alliot-Licht, B.
Human Dental Pulp Stem Cells Cultured in Serum-Free Supplemented Medium. ,
Front Physiol
 4 , 357 , 2013
Cells
Dental pulp stem
Enzymes
Medium
DMEM/F12
Miscellaneous
Human
Human
Lee, Y. , Lee, J. , Park, H. , Lim, Y. , Lee, J. , Wang, S. and Lee, B.
Isolation of Mesenchymal Stromal Cells (MSCs) from Human Adenoid Tissue. ,
Cell Physiol Biochem
 31 , 513-24 , 2013
Cells
Mesenchymal stromal
Enzymes
Medium
PBS
Miscellaneous
Human
Human
Ozdener, H. , Spielman, A. and Rawson, N.
Isolation and Culture of Human Fungiform Taste Papillae Cells ,
J Vis Exp
 63 , e3730 , 2012
Cells
Fungiform taste papillae
Enzymes
Medium
see reference
Miscellaneous
Human
Human
Pauly, S. , Klatte, F. , Strobel, C. , Schmidmaier, G. , Greiner, S. and Scheibel, M. Wildemann B
Characterization of Tendon Cell Cultures of the Human Rotator Cuff. ,
Eur Cell Mater
 20 , 84-97 , 2010
Cells
Tendon cells
Enzymes
Medium
DMEM/Hams F12
Miscellaneous
Human
Human
Panetta, N. , Gupta, D. , Kwan, M. , Wan, D. , Commons, G. and Longaker, M.
Tissue Harvest by Means of Suction-Assisted or Third-Generation Ultrasound-Assisted Lipoaspiration Has No Effect on Osteogenic Potential of Human Adipose-Derived Stromal Cells. ,
Plast Reconstr Surg
 124 , 65 , 2009
Cells
Adipose derived stromal
Enzymes
Medium
DMEM
Miscellaneous
Human
Human
Nakamura, T. , Endo, K. and Kinoshita, S.
Identification of Human Oral Keratinocyte Stem/Progenitor Cells by Neurotrophin Receptor p75 and the Role of Neurotrophin/p75 Signaling. ,
Stem Cells
 25 , 628-38 , 2007
Cells
Oral epithelial
Enzymes
Neutral Protease : 1.2 u/ml
Trypsin : 0.05%
Medium
PBS
Miscellaneous
Human
Human
Patino WillmarD, Kang Ju-Gyeong, Matoba Satoaki, Mian OmarY, Gochuico BernadetteR, Hwang PaulM.
Atherosclerotic plaque macrophage transcriptional regulators are expressed in blood and modulated by tristetraprolin ,
Circ Res
 98 , 1282-9 , 2006
Cells
Carotid artery plaque macrophage
Enzymes
Medium
HBSS
Miscellaneous
Human
Human
Rafiee, P. , Ogawa, H. , Heidemann, J. , Li, M. , Aslam, M. , Lamirand, T. , Fisher, P. , Graewin, S. , Dwinell, M. , Johnson, C. , Shaker, R. and Binion, D.
Isolation and Characterization of Human Esophageal Microvascular Endothelial Cells: Mechanisms of Inflammatory Activation. ,
Am J Physiol Gastrointest Liver Physiol
 285 , G1277 , 2003
Cells
Esophageal microvascular endothelial
Enzymes
Medium
MCDB-131
Miscellaneous
Human
Smoot, D. , Sewchand, J. , Young, K. , Desbrodes, B. , Allen, C. and Naab, T.
A Method for Establishing Primary Cultures of Human Gastric Epithelial Cells ,
Meth Cell Sci
 22 , 133 , 2000
Cells
Gastric epithelial cells
Enzymes
Medium
L-15
Miscellaneous
Human
Human
Chen V, Croft D, Purkis P, Kramer IM.
Co-culture of synovial fibroblasts and differentiated U937 cells is sufficient for high interleukin-6 but not interleukin-1beta or tumour necrosis factor-alpha release. ,
Br J Rheumatol
 37 , 148-56 , 1998
Cells
Synoviocytes
Enzymes
Collagenase : 0.2%
Medium
DMEM/F12
Miscellaneous
Human
Human
Lanas. A. , Nerin, J. , Esteva, F. and Sainz, R.
Non-Steroidal Anti-Inflammatory Drugs and Prostaglandin Effects on Pepsinogen Secretion by Dispersed Human Peptic Cells. ,
Gut
 36 , 657-63 , 1995
Cells
Peptic cells
Enzymes
Medium
Ringer solution
Miscellaneous
Human
Human
Stern MH, Dreizen S, Mackler BF, Levy BM.
Isolation and characterization of inflammatory cells from the human periapical granuloma ,
J Dent Res
 61 , 1408-12 , 1982
Cells
Periapical granuloma
Enzymes
CLSPA : 0.25%
Medium
RPMI-1640
Miscellaneous
Rat
Rat, Lewis, M
Jain, S. , Tran, T. and Amiji, M.
Macrophage Repolarization with Targeted Alginate Nanoparticles Containing IL-10 Plasmid DNA for the Treatment of Experimental Arthritis. ,
Biomaterials
 61 , 162-77 , 2015
Cells
Synovial macrophages
Enzymes
Collagenase Type 1 : 250 u/ml
Medium
DMEM
Miscellaneous
Rat
Rat, SD, 6-8 month
Zhou, B. , Zhou, Y. and Tang, K.
The Effects of Substance P on Pluripotent Tendon Cells: an In Vitro and In Vivo Study. ,
J Musculoskelet Neuronal Interact
 14 , 349-58 , 2014
Cells
Pluripotent tendon
Enzymes
Medium
DMEM
Miscellaneous
Rat
Rat, male, 250-350g
Silver, N. , Proctor, GB, Arno, M. and Carpenter GH
Activation of mTOR Coincides with Autophagy During Ligation-induced Atrophy in the Rat Submandibular Gland. ,
Cell Death Dis
 1 , e14 , 2010
Cells
Submandibular acinar
Enzymes
Collagenase : 0.05%
Medium
PBS
Miscellaneous
Rat
Rat, SD, 8-10 week
Lin, A. , Hokugo, A. , Choi, J. and Nishimura, I.
Small Cytoskeleton-Associated Molecule, Fibroblast Growth Factor Receptor 1 Oncogene Partner 2/Wound Inducible Transcript-3.0 (FGFR1OP2/wit3.0), Facilitates Fibroblast-Driven Wound Closure. ,
Am J Pathol
 176 , 108-21 , 2010
Cells
Oral fibroblasts
Enzymes
Collagenase Type 1 : 200 u/ml
Medium
DMEM
Miscellaneous
Rat
Rat, Wistar, 7-9 week
Kaneko, N. , Rikimaru, T. , Fujimura, T. , Mori, S. , Hidaka, S. and Kaya, H.
Preparation of Rat Gingival Mitochondria with an Improved Isolation Method. ,
Int J Dent
 2010 , 275103 , 2010
Cells
Gingival mitochondria
Enzymes
Collagenase Type 1 : 0.115-0.130 %
Medium
HBSS
Miscellaneous
Rat
Rat, 8-10 week
Moghaddami, M. , Cleland, L. and Mayrhofer, G.
MHC II+ CD45+ Cells from Synovium-Rich Tissues of Normal Rats: Phenotype, Comparison with Macrophage and Dendritic Cell Lineages and Differentiation into Mature Dendritic Cells In Vitro. ,
Int Immunol
 17 , 1103 , 2005
Cells
Synovial cells
Enzymes
Collagenase Type 1 : 250 u/ml
Medium
RPMI 1640

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.