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Tissue References Search Tool

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Skin
Human
Human, 18-50 years
Bell, C. and Quinton, P.
Effects of Media Buffer Systems on Growth and Electrophysiologic Characteristics of Cultured Sweat Duct Cells ,
In Vitro Cell Dev Biol
 27 , 47 , 1991
Cells
Sweat duct
Enzymes
Collagenase : 0.03%
Medium
MEM
Skin
Human
Human
Limat, A. , Hunziker, T. , Boillat, C. , Noser, F. and Wiesmann, U.
Postmitotic Human Dermal Fibroblasts Preserve Intact Feeder Properties for Epithelial Cell Growth After Long-Term Cryopreservation ,
In Vitro Cell Dev Biol
 26 , 709 , 1990
Cells
Fibroblasts
Enzymes
Trypsin : 0.25%
Medium
CMF solution
Skin
Human
Human
Krouse, M. , Hagiqara, G. , Chen, J. , Lewiston, N. and Wine, J.
Ion Channels in Normal Human and Cystic Fibrosis Sweat Gland Cells ,
Am J Physiol
 257 , C129 , 1989
Cells
Sweat gland
Enzymes
Medium
(see reference)
Skin
Human
Human, ages 16-30
Pedersen, P.
Human Sweat Duct Cells in Primary Culture. Basic Bioelectric Properties of Cultures Derived From Normals and Patients with Cystic Fibrosis ,
In Vitro Cell Dev Biol
 25 (4) , 342 , 1989
Cells
Human sweat duct
Enzymes
Medium
RPMI 1640
(see reference)
Skin
Human
Human
Peacocke, M. , Yaar, M. , Mansur, C. , Chao, M. and Gilchrest, B.
Induction of Nerve Growth Factor Receptors on Cultured Human Melanocytes ,
Proc Natl Acad Sci U S A
 85 , 5282 , 1988
Cells
Melanocytes, skin/ foreskin
Enzymes
Trypsin : 0.25%
Medium
PBS
Skin
Human
Human
Lee, C. , Carpenter, F. , Coaker, T. and Kealey, T.
The Primary Culture of Epithelia From the Secretory Coil and Collecting Duct of Normal Human and Cystic Fibrotic Eccrine Sweat Glands ,
J Cell Sci
 83 , 103 , 1986
Cells
Human sweat glands
Enzymes
Collagenase : 0.2%
Medium
HBSS
Skin
Human
Human
Dover, R. and Potten, C.
Cell Cycle Kinetics of Cultured Human Epidermal Keratinocytes ,
J Invest Dermatol
 80 , 423 , 1983
Cells
Keratinocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Human
Human, 18-30 years, male, female
McCoy, B. , Galdun, J. and Cohen, I.
Effects of Density and Cellular Aging On Collagen Synthesis and Growth Kinetics in Keloid and Normal Skin Fibroblasts ,
In Vitro
 18 (1) , 79 , 1982
Cells
Fibroblasts
Enzymes
Trypsin : 0.1%
Medium
HBSS
Skin
Human
Human, adult
Alitalo K, Kuismanen E, Myllyla R, Kiistala U, Asko-Seljavaara S, Vaheri A.
Extracellular matrix proteins of human epidermal keratinocytes and feeder 3T3 cells ,
J Cell Biol
 94 , 497-505 , 1982
Cells
Epidermal keratinocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.2%
Medium
Eagle's MEM
Skin
Human
Human, fetal
Davies, P. and Kerr, C.
Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts ,
Biochim Biophys Acta
 712 , 26 , 1982
Cells
Smooth muscle, fibroblasts
Enzymes
Trypsin : 0.055%
Medium
DMEM
Skin
Human
Human, newborn
Liu, S. , Eaton, M. and Karasek, M.
Growth Characteristics of Human Epidermal Keratinocytes from Newborn Foreskin in Primary and Serial Cultures ,
In Vitro
 15 (10) , 813 , 1979
Cells
Keratinocytes
Enzymes
Trypsin : 0.3%
Medium
DMEM
Skin
Human
Human
Rheinwald, J. and Green, H.
Serial Cultivation of Strains of Human Epidermal Kerotinocytes: The Formation of Keratinizing Colonies from Single Cells ,
Cell
 6 , 331 , 1975
Cells
Keratinocytes
Enzymes
Trypsin : 0.25%
Medium
(see reference)
Skin
Rat
Rat, Wistar, 4-8 day
Sugihara, H. and Toda, S
Primary Tissue Intact and Dissociated Cell Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 3A:2.1 , 1995
Cells
Dermal fibroblasts and keratinocytes
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Skin
Rat
Rat, SD, male, 40-60 days
Laurent, S. , Mednieks, M. and Rosenfield, R.
Growth of Sebaceous Cells in Monolayer Culture ,
In Vitro Cell Dev Biol
 28 , 83 , 1992
Cells
Sebaceous
Enzymes
Trypsin : 0.2%
Medium
DMEM with FBS
PBS
Skin
Rat
Rat, neonatal, 12-24 hr post partum
Acheson, A. , Barker, P. , Alderson, R. , Miller, F. and Murphy, R.
Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF ,
Neuron
 7 , 265 , 1991
Cells
Fibroblasts
Enzymes
Trypsin : 0.2%
Medium
HEPES buffered DMEM
Skin
Rat
Rat, albino, one day old, CFN
Vaughan, F. , Gray, R. and Bernstein, I.
Growth and Differentiation of Primary Rat Keratinocytes on Synthetic Membranes ,
In Vitro Cell Dev Biol
 22 (3) , 141 , 1986
Cells
Keratinocytes
Enzymes
Trypsin : 1%
Medium
EBSS
Stem
Human
Human
Alstrup, T. , Eijken, M. , Bohn, A. , Moller, B. and Damsgaard, T.
Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat. ,
Curr Protoc Stem Cell Biol
 48 , e68 , 2019
Cells
Adipose derived stem
Enzymes
Collagenase Type 4 : 150-300 u/ml
Medium
RPMI-1640
Stem
Human
Human
Yam, G. , Teo, E. , Setiawan, M. , Lovatt, M. , Yusoff, N. , Fuest, M. , Goh, B. and Mehta, J.
Postnatal Periodontal Ligament as a Novel Adult Stem Cell Source for Regenerative Corneal Cell Therapy. ,
J Cell Mol Med
 , 2018
Cells
Postnatal periodontal ligament
Enzymes
Medium
DMEM/F12
Stem
Human
Human
Matsumura, E. , Tsuji, K. , Komori, K. , Koga, H. , Sekiya, I. and Muneta, T.
Pretreatment with IL-1β Enhances Proliferation and Chondrogenic Potential of Synovium-Derived Mesenchymal Stem Cells. ,
Cytotherapy
 19 , 181-193 , 2017
Cells
Synovial mesenchymal stem
Enzymes
Collagenase : 0.3%
Medium
MEM
Stem
Human
Human
Clark, K. , Fierro, F. , Ko, E. , Walker, N. , Arzi, B. , Tepper, C. , Dahlenburg, H. , Cicchetto, A. , Koi, A. , Marsh, L. , Murphy, W. , Fazel, N. and Borjesson, D.
Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome. ,
Stem Cell Res Ther
 8 , 69 , 2017
Cells
Adipose derived mesenchymal stem
Enzymes
Medium
PBS
Stem
Human
Human
de Witte, S. , Lambert, E. , Merino, A. , Strini, T. , Douben, H. , O'Flynn, L. , Elliman, S. , de Klein, A. , Newsome, P. , Baan, C. and Hoogduijn, M.
Aging of Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stromal Cells During Expansion. ,
Cytotherapy
 19 , 798-807 , 2017
Cells
Umbilical cord mesenchymal stromal
Enzymes
Medium
MEM
Stem
Human
Human
Oppliger, B. , Joerger-Messerli, M. , Simillion, C. , Mueller, M. , Surbek, D. and Schoeberlein, A.
Mesenchymal Stromal Cells from Umbilical Cord Wharton's Jelly Trigger Oligodendroglial Differentiation in Neural Progenitor Cells Through Cell-to-Cell Contact. ,
Cytotherapy
 19 , 829-838 , 2017
Cells
Wharton jelly mesenchymal stromal
Enzymes
Collagenase Type 2 : 270 u/ml
Medium
DMEM/F12
Stem
Human
Human
Rigamonti, A. , Repetti, G. , Sun, C. , Price, F. , Reny, D. , Rapino, F. , Weisinger, K. , Benkler, C. , Peterson, Q. , Davidow, L. , Hansson, E. and Rubin, L.
Large-Scale Production of Mature Neurons from Human Pluripotent Stem Cells in a Three-Dimensional Suspension Culture System. ,
Stem Cell Reports
 6 , 993-1008 , 2016
Cells
Motor neurons
Enzymes
Papain : 20 u/ml
Medium
EBSS
Stem
Human
Human
Randelli, P. , Menon, A. , Ragone, V. , Creo P. , Alfieri, M. , Perucca, O. , Banfi, G. , Cabitza, P. , Tettamanti, G. and Anastasia, L.
Effects of the Pulsed Electromagnetic Field PST on Human Tendon Stem Cells: a Controlled Laboratory Study. ,
BMC Complement Altern Med
 16 , 293 , 2016
Cells
Tendon stem
Enzymes
Medium
MEM
Stem
Human
Human
Cheng, N. , Hsieh, T. , Lai, H. and Young, T.
High Glucose-Induced Reactive Oxygen Species Generation Promotes Stemness in Human Adipose-Derived Stem Cells ,
Cytotherapy
 18 , 371-83 , 2016
Cells
Adipose stem
Enzymes
Medium
DMEM/F12
Stem
Human
Human
Lim, J. , Razi, Z. , Law, J. , Nawi, A. , Idrus, R. and Ng, M.
MSCs can be Differentially Isolated From Maternal, Middle and Fetal Segments of the Human Umbilical Cord. ,
Cytotherapy
 18 , 1493-1502 , 2016
Cells
Wharton jelly mesenchymal stromal
Enzymes
Medium
DMEM
Stem
Human
Human
Mothe, A. and Tator, C.
Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord. ,
J Vis Exp
 , e52732 , 2015
Cells
Spinal cord neural progenitor
Enzymes
PDS kit : with modifications
Medium
EBSS
Stem
Human
Human
Wu, Z. , Wang, J. , Dong, R. , Wang, L. , Fan, Z. , Liu, D. and Wang, S.
Depletion of MEIS2 Inhibits Osteogenic Differentiation Potential of Human Dental Stem Cells. ,
Int J Clin Exp Med
 8 , 7220-30 , 2015
Cells
Dental mesenchymal stem
Enzymes
Medium
DMEM
Stem
Human
Human
Kusuma, G. , Menicanin, D. , Gronthos, S. , Manuelpillai, U. , Abumaree, M. , Pertile, M. , Brennecke, S. and Kalionis, B.
Ectopic Bone Formation by Mesenchymal Stem Cells Derived from Human Term Placenta and the Decidua. ,
PLoS ONE
 10 , e0141246 , 2015
Cells
Decidua mesenchymal stem
Enzymes
Medium
PBS
Stem
Human
Human
Satish, L. , Krill-Burger, J. , Gallo, P. , Etages, S. , Liu, F. , Philips, B. , Ravuri, S. , Marra, K. , LaFramboise, W. , Kathju, S. and Rubin, J.
Expression Analysis of Human Adipose-Derived Stem Cells During In Vitro Differentiation to an Adipocyte Lineage. ,
BMC Med Genomics
 8 , 41 , 2015
Cells
Adipose derived stem cells
Enzymes
Medium
HBSS
Stem
Human
Human
Charville, G. , Cheung, T. , Yoo, B. , Santos, P. , Lee, G. , Shrager, J. and Rando, T.
Ex Vivo Expansion and In Vivo Self-Renewal of Human Muscle Stem Cells. ,
Stem Cell Reports
 5 , 621 , 2015
Cells
Skeletal muscle stem
Enzymes
Collagenase Type 2 : 750 u/ml
Neutral Protease : 2 u/ml
Medium
Ham's F10
Stem
Human
Human
Lee, S. , Park, B. , Kim, J. , Jekarl, D. , Choi, H. , Lee, S. , Kim, M. , Kim, Y. and Park, M.
The Effect of Fibroblast Growth Factor on Distinct Differentiation Potential of Cord Blood-Derived Unrestricted Somatic stem Cells and Wharton's Jelly-Derived Mesenchymal Stem/Stromal Cells. ,
Cytotherapy
 17 , 1723-31 , 2015
Cells
Wharton jelly mesenchymal stem
Enzymes
Medium
PBS
Stem
Human
Human
Choudhery,M, Badowski, M, Muise, A. and Harris, D
Effect of Mild Heat Stress on the Proliferative and Differentiative Ability of Human Mesenchymal Stromal Cells. ,
Cytotherapy
 17 , 359-68 , 2015
Cells
Mesenchymal stromal
Enzymes
Medium
PBS
Stem
Human
Human
Najar, M, Rodrigues, R, Buyl, K, Branson, S, Vanhaecke, T, Lagneaux, L, Rogiers, V. and De Kock, J
Proliferative and Phenotypical Characteristics of Human Adipose Tissue-Derived Stem Cells: Comparison of Ficoll Gradient Centrifugation and Red Blood Cell Lysis Buffer Treatment Purification Methods. ,
Cytotherapy
 16 , 1220-8 , 2014
Cells
Mesenchymal stromal
Enzymes
Medium
PBS
Stem
Human
Human
Douvaras, P. , Wang, J. , Zimmer, M. , Hanchuk, S. , O'Bara, M. , Sadiq, S. , Sim, F. , Goldman, J. and Fossati, V.
Efficient Generation of Myelinating Oligodendrocytes from Primary Progressive Multiple Sclerosis Patients by Induced Pluripotent Stem Cells. ,
Stem Cell Reports
 3 , 250 , 2014
Cells
Skin fibroblasts
Enzymes
Collagenase Type 1 : 1000 u/ml
Medium
DMEM
Stem
Human
Human
Hang, H. , Yu, Y. , Wu, N. , Huang, Q. , Xia, Q. and Bian, J.
Induction of Highly Functional Hepatocytes from Human Umbilical Cord Mesenchymal Stem Cells by HNF4α Transduction. ,
PLoS ONE
 9 , e104133 , 2014
Cells
Umbilical mesenchymal stem
Enzymes
Trypsin : 0.25%
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.