For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.

Quick Order

Tissue References Search Tool

Search
Stem
Human
Human
Zuk, P. , Zhu, M. , Ashjian, P. , De Ugarte, D. , Huang, J. , Mizuno, H. , Alfonso, Z. , Fraser, J. , Benhaim, P. and Hedrick, M.
Human adipose tissue is a source of multipotent stem cells ,
Mol Biol Cell
 13 , 4279-95 , 2002
Cells
Processed lipoaspirate cells
Enzymes
Medium
PBS
Stem
Human
Human
Zhang, S. , Wernig, M. , Duncan, I. , Brustle, O. and Thomson, J.
In Vitro Differentiation of Transplantable Neural Precursors from Human Embryonic Stem Cells ,
Nat Biotechnol
 19 , 1129 , 2001
Cells
Stem, embryonic
Enzymes
Neutral Protease : 0.01% - 0.02%
Medium
DMEM
Stem
Human
Human, adult
Kambe, N, Kambe, M, Kochan, J. and Schwartz ,L.
Human Skin-derived Mast Cells Can Proliferate While Retaining Their Characteristic Functional and Protease Phenotypes ,
Blood
 97 , 2045 , 2001
Cells
Human skin mast cells
Enzymes
Medium
HBSS
Stem
Human
Human
Uchida, N. , Buck, D. , He, D. , Reitsma, M. , Masek, M. , Phan, T. , Tsukamoto, A. , Gage, F. and Weissman, I.
Direct Isolation of Human Central Nervous System Stem Cells. ,
Proc Natl Acad Sci U S A
 97 , 14720 , 2000
Cells
Central nervous system stem
Enzymes
Collagenase : 0.1%
Hyaluronidase : 0.1%
Medium
HBSS
Stem
Human
Human
Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM.
Embryonic stem cell lines derived from human blastocysts ,
Science
 282 , 1145-7 , 1998
Cells
Embryonic stem
Enzymes
Medium
DMEM
Stem
Human
Human
Alessandri Giulio, Pagano Stefano, Bez Alessandra, Benetti Anna, Pozzi Stefano, Iannolo Gioacchin, Baronio Manuela, Invernici Gloria, Caruso Arnaldo, Muneretto Claudio, Bisleri Gianluigi, Parati Eugenio.
Isolation and culture of human muscle-derived stem cells able to differentiate into myogenic and neurogenic cell lineages ,
Lancet
 364 , 1872-83
Cells
Muscle-derived stem cells
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Stem
Rat
Rat, 9 week
Ohta, Y. , Hamaguchi, A. , Ootaki, M. , Watanabe, M. , Takeba, Y. , Iiri, T. , Matsumoto, N. and Takenaga, M.
Intravenous Infusion of Adipose-Derived Stem/Stromal Cells Improves Functional Recovery of Rats with Spinal Cord Injury. ,
Cytotherapy
 19 , 839-848 , 2017
Cells
Adipose stromal
Enzymes
Collagenase : 0.1%
Medium
DMEM
Stem
Rat
Rat
Jiang, H. , Zhang, Y. , Tian, K. , Wang, B. and Han, S.
Amelioration of Experimental Autoimmune Encephalomyelitis Through Transplantation of Placental Derived Mesenchymal Stem Cells. ,
Sci Rep
 7 , 41837 , 2017
Cells
Placental mesenchymal
Enzymes
Medium
DMEM
Stem
Rat
Rat, SD, 2-3 month
Dayer, D. , Tabar, M. , Moghimipour, E. , Tabandeh, M. , Ghadiri, A. , Bakhshi, E. , Orazizadeh, M. and Ghafari, M.
Sonic Hedgehog Pathway Suppression and Reactivation Accelerates Differentiation of Rat Adipose-Derived Mesenchymal Stromal Cells Toward Insulin-Producing Cells ,
Cytotherapy
 19 , 937-946 , 2017
Cells
Mesenchymal stromal
Enzymes
Medium
DMEM
Stem
Rat
Rat, 6-8 week
Mothe, A. and Tator, C.
Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord. ,
J Vis Exp
 , e52732 , 2015
Cells
Spinal cord neural progenitor
Enzymes
PDS kit : with modifications
Medium
EBSS
Stem
Rat
Rat
Emre, E, Yuksel, N, Duruksu, G, Pirhan, D, Subasi, C, Erman, G. and Karaoz, E
Neuroprotective Effects of Intravitreally Transplanted Adipose Tissue and Bone Marrow-Derived Mesenchymal Stem Cells in an Experimental Ocular Hypertension Model. ,
Cytotherapy
 17 , 543-59 , 2015
Cells
Mesenchymal stem
Enzymes
Medium
DMEM
Stem
Rat
Rat. SD, 5 month
Veronesi, F, Torricelli, P, Della, B, Pagani, S. and Fini, M
In Vitro Mutual Interaction Between Tenocytes and Adipose-Derived Mesenchymal Stromal Cells. ,
Cytotherapy
 17 , 215-23 , 2015
Cells
Mesenchymal stromal
Enzymes
Medium
DMEM
Stem
Rat
Rat, adult
Duarte, S. , Momier, D. , Baque, P. , Casanova, V. , Loubat, A. , Samson, M. , Guigonis, J. , Staccini, P. , Saint-Paul, M. , De Lima, M. , Carle, G. and Pierrefite-Carle, V.
Preventive Cancer Stem Cell-Based Vaccination Reduces Liver Metastasis Development in a Rat Colon Carcinoma Syngeneic Model. ,
Stem Cells
 31 , 423-32 , 2013
Cells
Tumor
Enzymes
Medium
DMEM/F12
Stem
Rat
Rat, SD, neonatal
Liu, Z. , Wang, H. , Zhang, Y. , Zhou, J. , Lin, Q. , Wang, Y. , Duan, C. , Wu, K. and Wang, C.
Efficient Isolation of Cardiac Stem Cells from Brown Adipose. ,
J Biomed Biotechnol
 2010 , 104296 , 2010
Cells
Brown adipocytes
Enzymes
Medium
PBS
Stem
Rat
Rat
Wei, X. , Du, Z. , Zhao, L. , Feng, D. , Wei, G. , He, Y. , Tan, J. , Lee, W. , Hampel, H. , Dodel, R. , Johnstone, B. , March, K. , Farlow, M. and Du, Y.
IFATS Collection: The Conditioned Media of Adipose Stromal Cells Protect Against Hypoxia-Ischemia-Induced Brain Damage In Neonatal Rats. ,
Stem Cells
 27 , 478 , 2009
Cells
Adipose derived stem
Enzymes
Medium
DMEM
Stem
Rat
Rat, SD, newborn
Zeng Yuan-Shan, Ding Ying, Wu Li-Zhi, Guo Jia-Song, Li Hai-Biao, Wong Wai-Man, Wu Wu-Tian.
Co-transplantation of schwann cells promotes the survival and differentiation of neural stem cells transplanted into the injured spinal cord ,
Dev Neurosci
 27 , 20-6 , 2005
Cells
Neutral stem cells, Schwann cells
Enzymes
Trypsin : 0.25%
Collagenase : 0.16%
Medium
DMEM/F12
Stem
Rat
Rat, 2 month
Mothe, A. , Kulbatski, I. , Van Bendegem, R. , Lee, L. , Kobayashi, E. , Keating, A. and Tator, C.
Analysis of Green Fluorescent Protein Expression in Transgenic Rats for Tracking Transplanted Neural Stem/Progenitor Cells ,
J Histochem Cytochem
 53(10) , 1215 , 2005
Cells
Spinal cord progenitor cells
Hybrid toxins
Enzymes
PDS kit : see reference
Medium
Neurobasal A
Stem
Rat
Rat, male, 200-250 g
Gobbel GT, Choi SJ, Beier S, Niranjan A.
Long-term cultivation of multipotential neural stem cells from adult rat subependyma ,
Brain Res
 980 , 221 , 2003
Cells
Neural stem cells
Enzymes
Papain : 0.09%
Medium
EBSS
Stem
Rat
Rat, postnatal, adult
Kruger, G. , Mosher, J. , Bixby, S. , Joseph, N. , Iwashita, T. and Morrison, S.
Neural Crest Stem Cells Persist in the Adult Gut but Undergo Changes in Self-Renewal, Neuronal Subtype Potential, and Factor Responsiveness. ,
Neuron
 35 , 657 , 2002
Cells
Gut neural crest stem
Enzymes
Trypsin : 0.025%
Medium
HBSS
Stem
Rat
Rat, SD, embyronic
Bixby, S. , Kruger, G. , Mosher, J. , Joseph, N. and Morrison, S.
Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity. ,
Neuron
 35 , 643 , 2002
Cells
Sciatic nerve and gut neural crest stem
Enzymes
Trypsin : 0.005%
Medium
HBSS
Stem
Rat
Rat, adult
Palmer, T. , Markakis, E. , Willhoite, A. , Safar, F. and Gage, F.
Fibroblast Growth Factor-2 Activates a Latent Neurogenic Program in Neural Stem Cells from Diverse Regions of the Adult CNS ,
J Neurosci
 19 , 8487 , 1999
Cells
Neural stem cells
Enzymes
Papain : 2.5 u/ml
Deoxyribonuclease I : 250 u/ml
Neutral Protease : 1 u/ml
Medium
DMEM/F-12
Stem
Rat
Rat
Ray, J. and Gage, F.
Neural Stem Cell Isolation, Characterization and Transplantation , Modern Techniques in Neuroscience Research , Johansson, H. and Windhorst, U. , Springer-Verlag New York , 339 , 1999
Cells
Central nervous system stem cells
Enzymes
Papain : 0.01%
Medium
DMEM/F-12
Stem
Rat
Rat (also mice)
Johansson, C. , Momma, S. , Clarke, D. , Risling, M. , Lendahl, U. and Frisen, J
Identification of a Neural Stem Cell in the Adult Mammalian Central Nervous System ,
Cell
 96 , 25 , 1999
Cells
Spinal cord
Enzymes
Trypsin : 0.133%
Medium
HBSS and PIPES
Stem
Rat
Rat, SD
Morrison, S. , White, P. , Zock, C. and Anderson, D.
Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells ,
Cell
 96 , 737 , 1999
Cells
Sciatic nerves
Enzymes
Trypsin : 0.025%
Medium
L-15 medium
(see reference)
Stem
Rat
Rat, embryonic
Maric, D. , Maric, I. and Barker, J.
Buoyant Density Gradient Fractionation and Flow Cytometric Analysis of Embryonic Rat Cortical Neurons and Progenitor Cells. ,
Methods
 16 , 247 , 1998
Cells
Neurons and progenitor
Enzymes
Papain : 20 u/ml
Medium
EBSS
Stem
Rat
Rat
Stemple, D. and Anderson, D.
Isolation of a Stem Cell for Neurons and Glia from the Mammalian Neural Crest ,
Cell
 71 , 973 , 1992
Cells
Stem, neural crest
Enzymes
Collagenase : 0.075%
Medium
Ringer's solution
Thymus
Human
Human
Vandenabeele S, Hochrein H, Mavaddat N, Winkel K, Shortman K.
Human thymus contains 2 distinct dendritic cell populations ,
Blood
 97 , 1733-41 , 2001
Cells
Dentritic
Enzymes
Medium
RPMI 1640
Thymus
Rat
Rat, postnatal
Bonfanti, P. , Claudinot, S. , Amici, A. , Farley, A. , Blackburn, C. and Barrandon, Y.
Microenvironmental Reprogramming of Thymic Epithelial Cells to Skin Multipotent Stem Cells. ,
Nature
 466 , 978 , 2010
Cells
Thymic
Enzymes
Trypsin : 0.05%
Medium
HBSS
Thymus
Rat
Rat
Masuda, A. and Matsuyama, M
Epithelial Cell Lines From Rat Thymoma and Rat Thymus , Cell & Tissue Culture: Laboratory Procedures Vol. 2 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 21C:4.1 , 1995
Cells
Thymic epithelial
Enzymes
Medium
DMEM
Thymus
Rat
Rat (ACI/NMs X BUF/Mna) F1, male, 28 months
Rat, (ACI/MNs) male, 8 weeks
(also bovine, adult)
Masuda, A. , Ohtsuka, K. and Matsuyama, M.
Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus ,
In Vitro Cell Dev Biol
 26 , 713 , 1990
Cells
Epthelial
Enzymes
Medium
Eagle's MEM Serum-free
Thyroid/Parathyroid
Human

Human

Yi, S. , Kim, H. , Koo, B. , Lee, S. , Choi, J. and Kang, Y.
Efficient Dissociation Protocol for Generation of Single Cell Suspension from Human Thyroid Tissue for Single Cell RNA Sequencing. , Endocrinol Metab (Seoul) Vol. 37 , 698-700 , 2022
Cells
Thyroid normal and tumor
Enzymes
Trypsin : 0.125%
Collagenase Type 4 : 300 u/ml
Medium
RPMI-1640
Thyroid/Parathyroid
Human
Human
Patel, V. , Logan, A. , Watkinson, J. , Uz-Zaman, S. , Sheppard, M. , Ramsden, J. and Eggo, M.
Isolation and Characterization of Human Thyroid Endothelial Cells. ,
Am J Physiol Endocrinol Metab
 284 , E168 , 2003
Cells
Endothelial
Enzymes
Medium
DMEM
Thyroid/Parathyroid
Human
Human
Gianoukakis, A. , Cao, H. , Jennings, T. and Smith, T.
Prostaglandin Endoperoxide H Synthase Expression in Human Thyroid Epithelial Cells. ,
Am J Physiol Cell Physiol
 280 , C701 , 2001
Cells
Thyrocytes
Enzymes
Collagenase Type 1 : 130 u/ml
Neutral Protease : 0.5 u/ml
Medium
HBSS
Thyroid/Parathyroid
Human
Human
Howie, A. , Walker, S. , Akesson, B. , Arthur, J. and Beckett, G.
Thyroidal Extracellular Glutathione Peroxidase: A Potential Regulator of Thyroid-Hormone Synthesis. ,
Biochem J
 308 ( Pt 3) , 713 , 1995
Cells
Thyrocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.1%
Medium
EBSS
Thyroid/Parathyroid
Human
Human
Miller, R. , Hiraoka, T. , Nakamura, N. , Tenou, H. , Kopecky, K. , Jones, M. and Gould, M.
In Vitro Culture of Human Thyroid Cells; Methods and Application to Radiation Biology. ,
J Radiat Res
 26 , 269 , 1985
Cells
Thyroid
Enzymes
Collagenase : 300 u/ml
Medium
Ham's F-12/MEM
Thyroid/Parathyroid
Rat
Rat, Lewis, male, 4 week
Arauchi, A. , Shimizu, T. , Yamato, M. , Obara, T. and Okano, T.
Tissue-Engineered Thyroid Cell Sheet Rescued Hypothyroidism in Rat Models After Receiving Total Thyroidectomy Comparing with Nontransplantation Models. ,
Tissue Eng Part A
 15 , 3943 , 2009
Cells
Thyroid
Enzymes
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.