For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.

Quick Order

Tissue References Search Tool

Search
Brain
Human

Human

Nolle, A. , Dijken, I. , Waelti, C. , Calini, D. , Bryois, J. and Lezan, E.
Enrichment of Glial Cells From Human Post-mortem Tissue for Transcriptome and Proteome Analysis Using Immunopanning. ,
Front Cell Neurosci
 15 , 772011 , 2021
Enzymes
Papain : 20 u/ml
Medium
EBSS
Brain
Human

Human, fetal

Barbar, L. , Rusielewicz, T. , Zimmer, M. , Kalpana, K. and Fossati, V.
Isolation of Human CD49f Astrocytes and iPSC-Based Neurotoxicity Assays. , STAR Protoc Vol. 1 , 100172 , 2020
Cells
Astrocytes
Enzymes
PDS kit : see reference
Medium
EBSS
Brain
Human
Human, adult
Spaethling, J. , Na, Y. , Lee, J. , Ulyanova, A. , Baltuch, G. , Bell, T. , Brem, S. , Chen, H. , Dueck, H. , Fisher, S. , Garcia, M. , Khaladkar, M. , Kung, D. , Lucas, T. , O'Rourke, D. and Stefanik, D.
Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics. ,
Cell Rep
 18 , 791-803 , 2017
Cells
Neuronal
Enzymes
Papain : 20 u/ml
Medium
Neurobasal
Brain
Human
Human
Mizee, M. , Miedema, S. , van der Poel, M. , Adelia , S. , van Strien, M. , Melief, J. , Smolders, J. , Hendrickx, D. , Heutinck, K. , Hamann, J. and Huitinga, I.
Isolation of Primary Microglia from the Human Post-Mortem Brain: Effects of Ante- and Post-Mortem Variables. ,
Acta Neuropathol
 5 , 16 , 2017
Cells
Microglia
Enzymes
Collagenase Type 1 : 300 u/ml
Trypsin : 0.125%
Medium
DMEM
Brain
Human
Human
Rustenhoven, J. , Park Thomas, I. , Schweder, P. , Scotter, J. , Correia, J. , Smith, A. , Gibbons, H. , Oldfield, R. , Bergin, P. , Mee, E. , Faull, R. , Curtis, M. , Scott, G. and Dragunow, M.
Isolation of Highly Enriched Primary Human Microglia for Functional Studies. ,
Sci Rep
 6 , 19371 , 2016
Cells
Microglia
Enzymes
Neutral Protease : 10 u/ml
Papain : 2.5 u/ml
Medium
Hibernate A
Brain
Human
Human, fetal and mature
Zhang, Y. , Sloan, S. , Clarke, L. , Caneda, C. , Plaza, C. , Blumenthal, P. , Vogel, H. , Steinberg, G. , Edwards, M. , Li, G. , Duncan, J. , Cheshier, S. , Shuer, L. , Chang, E. , Grant, G. , Gephart, M. and Barres, B.
Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse. ,
Neuron
 89 , 37-53 , 2016
Cells
Astrocytes and neurons
Enzymes
Papain : 7.5-20 u/ml
Medium
RPMI
Brain
Human
Human
Scherpelz, K. , Lu, J.,Tycko, R. and Meredith, S.
Preparation of Amyloid Fibrils Seeded from Brain and Meninges ,
Methods Mol Biol
 1345 , 299 , 2016
Cells
Amyloid Fibrils
Enzymes
Medium
50mM Tris
Brain
Human
Human, 25-81 yr
Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,
A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
 17 , 30 , 2016
Cells
Brain tumor
Enzymes
Neutral Protease : 0.11 u/ml
Hyaluronidase : 1,000 u/ml
Medium
HBSS
Brain
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
Brain
Human
Human
Sauvageot, C. , Weatherbee, J. , Kesari, S. , Winters, S. , Barnes, J. , Dellagatta, J. , Ramakrishna, N. , Stiles, C. , Kung, A. , Kieran, M. and Wen, P.
Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells. , Neuro Oncol Vol. 11 , 109 , 2009
Cells
Tumor
Enzymes
Medium
see reference
Brain
Human
Human
Klegeris Andis, McGeer PatrickL.
Chymotrypsin-like proteases contribute to human monocytic THP-1 cell as well as human microglial neurotoxicity ,
Glia
 51 , 56-64 , 2005
Cells
Microglia
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Brain
Human
Human
Roher, A.E. , Palmer, K.C. , Chau, V. and Ball, M.J.
Isolation and Chemical Characterization of Alzheimer's Disease Paired Helical Filament Cytoskeletons:Differentiation from Amyloid Plaque Core Protein ,
J Cell Biol
 107 , 2703 , 1988
Cells
Neuronal
Enzymes
Deoxyribonuclease I : 10 µg/ml
Medium
Tris-HCl, 50 mM, CaCl2, 2 mM
Brain
Human
Human
Gerhart, D. Z. , Broderius, M. A. and Drewes, L. R.
Cutlured Human and Canine Endothelial Cells from Brain Microvessels ,
Brain Res Bull
 21 , 785 , 1988
Cells
Microvessels
Enzymes
Medium
DMEM
Brain
Human
Human, 15-54 years
Vinters, H.V. , Reave, S. , Costello, P. , Girvin, J.P. and Moore, S.A.
Isolation and Culture of Cells Derived From Human Cerebral Microvessels ,
Cell Tissue Res
 249 , 657 , 1987
Cells
Microvessels
Enzymes
Collagenase : 0.1%
Medium
Serum-free modified Lewis medium
Brain
Human

Human

Arlotta, P. , Quadrato, G. and Sherwood, L.
Long-term Culture and Electrophysiological Characterization of Human Brain Organoids , Protocol.OI Vol. https://doi.org/10.1038/protex.2017.049
Cells
Brain organoids
Muscle
Human
Human
Malysz, J. , Rovner, E. , Wake, R. and Petkov, G.
Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents. ,
J Vis Exp
 , 2020
Cells
Bladder smooth muscle
Enzymes
Collagenase Type 2 : 0.1-0.2%
Papain : 0.1-0.2%
Medium
Digestion solution (see reference)
Muscle
Human
Human
Garcia, S. , Tamaki, S. , Lee, S. , Wong, A. , Jose, A. , Dreux, J. , Kouklis, G. , Sbitany, H. , Seth, R. , Knott, P. , Heaton, C. , Ryan, W. , Kim, E. , Hansen, S. , Hoffman, W. and Pomerantz, J.
High-Yield Purification, Preservation, and Serial Transplantation of Human Satellite Cells. ,
Stem Cell Reports
 10 , 1160-1174 , 2018
Cells
Muscle satellite
Enzymes
Collagenase : 0.1%
Trypsin : 0.25%
Medium
DMEM
Muscle
Human
Human
Charville, G. , Cheung, T. , Yoo, B. , Santos, P. , Lee, G. , Shrager, J. and Rando, T.
Ex Vivo Expansion and In Vivo Self-Renewal of Human Muscle Stem Cells. ,
Stem Cell Reports
 5 , 621 , 2015
Cells
Skeletal muscle stem
Enzymes
Collagenase Type 2 : 750 u/ml
Neutral Protease : 2 u/ml
Medium
Ham's F10
Muscle
Human
Human
Lu, S. , Sun, X. , Hong, T. , Song, K. , Yang, S. and Wang, C.
Isolation and Culture of Smooth Muscle Cells from Human Acute Type A Aortic Dissection. ,
J Cardiothorac Surg
 8 , 83 , 2013
Cells
Smooth muscle
Enzymes
Medium
DMEM
Muscle
Human
Human, male
Stadler, G. , Chen, J. , Wagner, K. , Robin, J. , Shay, J. , Emerson, C. and Wright, W.
Establishment of Clonal Myogenic Cell Lines from Severely Affected Dystrophic Muscles - CDK4 Maintains the Myogenic Population. ,
Skelet Muscle
 1 , 12 , 2011
Cells
Myogenic
Enzymes
Medium
HBSS
Muscle
Human
Human
Nesti, L. , Jackson, W. , Shanti, R. , Koehler, S. , Aragon, A. , Bailey, J. , Sracic, M. , Freedman, B. , Giuliani, J. and Tuan, R.
Differentiation Potential of Multipotent Progenitor Cells Derived from War-Traumatized Muscle Tissue. ,
J Bone Joint Surg Am
 90 , 2390 , 2008
Cells
Muscle derived multiprogenitor cells
Enzymes
Medium
DMEM
Muscle
Human
Human
Moss, S. , Bates, M. , Parrino, P. and Woods, TC.
Isolation of Endothelial Cells and Vascular Smooth Muscle Cells from Internal Mammary Artery Tissue. ,
Ochsner J
 7 , 133 , 2007
Cells
Endothelial and vascular smooth muscle
Enzymes
Medium
HBSS
Muscle
Human
Human
Kimuli, M. , Eardley, I. and Southgate, J.
In Vitro Assessment of Decellularized Porcine Dermis as a Matrix for Urinary Tract Reconstruction. ,
BJU Int
 94 , 859 , 2004
Cells
Urinary tract smooth muscle
Enzymes
Collagenase Type 4 : 100 u/ml
Medium
DMEM
Muscle
Human
Human, female
Richardson, M. , Taylor, D. , Casey, M. , MacDonald, P. and Stull, J.
Biochemical Markers of Contraction in Human Myometrial Smooth Muscle Cells in Culture ,
In Vitro Cell Dev Biol
 23 , 21 , 1987
Cells
Smooth muscle
Myometrial
Enzymes
Deoxyribonuclease I : 0.015% and 0.007%
Medium
HBSS
Muscle
Human
Human, female
Casey, M. , MacDonald, P. , Mitchell, M. and Snyder, J.
Maintenance and Characterization of Human Myometrial Smooth Muscle Cells in Monolayer Culture ,
In Vitro
 20 , 396 , 1984
Cells
Smooth muscle
Myometrial
Enzymes
Medium
HBSS
Muscle
Human
Human, fetal
(also bovine)
Davies, P. and Kerr, C.
Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts ,
Biochim Biophys Acta
 712 , 26 , 1982
Cells
Smooth muscle, fibroblasts
Enzymes
Trypsin : 0.055%
Medium
DMEM
Muscle
Human
Human
Eskin, S. , Sybers, H. , Lester, J. , Navarro, L. , Gotto, A. and DeBakey, M.
Human Smooth Muscle Cells Cultured From Atherosclerotic Plaques and Uninvolved Vessel Wall ,
In Vitro
 17 (8) , 713 , 1981
Cells
Smooth muscle
Enzymes
Trypsin : 0.25%
Medium
DMEM
Muscle
Human
Human
(also rat, guinea-pig, chick, monkey)
Chamley-Campbell, J. , Campbell, G. and Ross, R.
The Smooth Muscle in Cell Culture ,
Physiol Res
 59 , 1 , 1979
Cells
Smooth muscle
Enzymes
Trypsin : 0.05% - 0.1%
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.