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Tissue References Search Tool

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Brain
Human

Human

Nolle, A. , Dijken, I. , Waelti, C. , Calini, D. , Bryois, J. and Lezan, E.
Enrichment of Glial Cells From Human Post-mortem Tissue for Transcriptome and Proteome Analysis Using Immunopanning. ,
Front Cell Neurosci
 15 , 772011 , 2021
Enzymes
Papain : 20 u/ml
Medium
EBSS
Brain
Human

Human, fetal

Barbar, L. , Rusielewicz, T. , Zimmer, M. , Kalpana, K. and Fossati, V.
Isolation of Human CD49f Astrocytes and iPSC-Based Neurotoxicity Assays. , STAR Protoc Vol. 1 , 100172 , 2020
Cells
Astrocytes
Enzymes
PDS kit : see reference
Medium
EBSS
Brain
Human
Human, adult
Spaethling, J. , Na, Y. , Lee, J. , Ulyanova, A. , Baltuch, G. , Bell, T. , Brem, S. , Chen, H. , Dueck, H. , Fisher, S. , Garcia, M. , Khaladkar, M. , Kung, D. , Lucas, T. , O'Rourke, D. and Stefanik, D.
Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics. ,
Cell Rep
 18 , 791-803 , 2017
Cells
Neuronal
Enzymes
Papain : 20 u/ml
Medium
Neurobasal
Brain
Human
Human
Mizee, M. , Miedema, S. , van der Poel, M. , Adelia , S. , van Strien, M. , Melief, J. , Smolders, J. , Hendrickx, D. , Heutinck, K. , Hamann, J. and Huitinga, I.
Isolation of Primary Microglia from the Human Post-Mortem Brain: Effects of Ante- and Post-Mortem Variables. ,
Acta Neuropathol
 5 , 16 , 2017
Cells
Microglia
Enzymes
Collagenase Type 1 : 300 u/ml
Trypsin : 0.125%
Medium
DMEM
Brain
Human
Human
Rustenhoven, J. , Park Thomas, I. , Schweder, P. , Scotter, J. , Correia, J. , Smith, A. , Gibbons, H. , Oldfield, R. , Bergin, P. , Mee, E. , Faull, R. , Curtis, M. , Scott, G. and Dragunow, M.
Isolation of Highly Enriched Primary Human Microglia for Functional Studies. ,
Sci Rep
 6 , 19371 , 2016
Cells
Microglia
Enzymes
Neutral Protease : 10 u/ml
Papain : 2.5 u/ml
Medium
Hibernate A
Brain
Human
Human, fetal and mature
Zhang, Y. , Sloan, S. , Clarke, L. , Caneda, C. , Plaza, C. , Blumenthal, P. , Vogel, H. , Steinberg, G. , Edwards, M. , Li, G. , Duncan, J. , Cheshier, S. , Shuer, L. , Chang, E. , Grant, G. , Gephart, M. and Barres, B.
Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse. ,
Neuron
 89 , 37-53 , 2016
Cells
Astrocytes and neurons
Enzymes
Papain : 7.5-20 u/ml
Medium
RPMI
Brain
Human
Human
Scherpelz, K. , Lu, J.,Tycko, R. and Meredith, S.
Preparation of Amyloid Fibrils Seeded from Brain and Meninges ,
Methods Mol Biol
 1345 , 299 , 2016
Cells
Amyloid Fibrils
Enzymes
Medium
50mM Tris
Brain
Human
Human, 25-81 yr
Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,
A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
 17 , 30 , 2016
Cells
Brain tumor
Enzymes
Neutral Protease : 0.11 u/ml
Hyaluronidase : 1,000 u/ml
Medium
HBSS
Brain
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
Brain
Human
Human
Sauvageot, C. , Weatherbee, J. , Kesari, S. , Winters, S. , Barnes, J. , Dellagatta, J. , Ramakrishna, N. , Stiles, C. , Kung, A. , Kieran, M. and Wen, P.
Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells. , Neuro Oncol Vol. 11 , 109 , 2009
Cells
Tumor
Enzymes
Medium
see reference
Brain
Human
Human
Klegeris Andis, McGeer PatrickL.
Chymotrypsin-like proteases contribute to human monocytic THP-1 cell as well as human microglial neurotoxicity ,
Glia
 51 , 56-64 , 2005
Cells
Microglia
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Brain
Human
Human
Roher, A.E. , Palmer, K.C. , Chau, V. and Ball, M.J.
Isolation and Chemical Characterization of Alzheimer's Disease Paired Helical Filament Cytoskeletons:Differentiation from Amyloid Plaque Core Protein ,
J Cell Biol
 107 , 2703 , 1988
Cells
Neuronal
Enzymes
Deoxyribonuclease I : 10 µg/ml
Medium
Tris-HCl, 50 mM, CaCl2, 2 mM
Brain
Human
Human
Gerhart, D. Z. , Broderius, M. A. and Drewes, L. R.
Cutlured Human and Canine Endothelial Cells from Brain Microvessels ,
Brain Res Bull
 21 , 785 , 1988
Cells
Microvessels
Enzymes
Medium
DMEM
Brain
Human
Human, 15-54 years
Vinters, H.V. , Reave, S. , Costello, P. , Girvin, J.P. and Moore, S.A.
Isolation and Culture of Cells Derived From Human Cerebral Microvessels ,
Cell Tissue Res
 249 , 657 , 1987
Cells
Microvessels
Enzymes
Collagenase : 0.1%
Medium
Serum-free modified Lewis medium
Brain
Human

Human

Arlotta, P. , Quadrato, G. and Sherwood, L.
Long-term Culture and Electrophysiological Characterization of Human Brain Organoids , Protocol.OI Vol. https://doi.org/10.1038/protex.2017.049
Cells
Brain organoids
Pancreas
Human
Human
Brissova, M. , Haliyur, R. , Saunders, D. , Shrestha, S. , Dai, C. , Blodgett, D. , Bottino, R. , Campbell-Thompson, M. , Aramandla, R. , Poffenberger, G. , Lindner, J. , Pan, F. , von Herrath, M. , Greiner, D. and Shultz, L.
α Cell Function and Gene Expression Are Compromised in Type 1 Diabetes. ,
Cell Rep
 22 , 2667-2676 , 2018
Cells
Islets
Enzymes
Collagenase Type 1 : 1,600u/isolation
Neutral Protease : 200 u/isolation
Deoxyribonuclease I : 12,000 u/isolation
Medium
RPMI
Pancreas
Human
Human
Cane, M. , Sutton, R. and Criddle, D.
Isolation of Human Pancreatic Acinar Cells From Rat and Human Pancreas ,
Pancreapedia
 20 , 1 , 2011
Cells
Acinar
Enzymes
CLSPA : 200 u/ml
Medium
See Reference
Pancreas
Human
Human
Li, C. , Heidt, D. , Dalerba, P. , Burant, C. , Zhang, L. , Adsay, V. , Wicha, M. , Clarke, M. and Simeone, D.
Identification of Pancreatic Cancer Stem Cells. ,
Cancer Res
 67 , 1030 , 2007
Cells
Pancreatic cancer stem cells
Enzymes
Collagenase Type 4 : 200 u/ml
Medium
medium 199
Pancreas
Human
Human, 25-55 yrs old
(also porcine)
Contractor, H. , Johnson, P. , Chadwick, D. , Robertson, G. and London, N.
The Effect of UW Solution and its Components on the Collagenase Digestion of Human and Porcine Pancreas ,
Cell Transplant
 4 , 615 , 1995
Cells
Islets
Enzymes
Collagenase : 0.4%
Medium
HBSS
Pancreas
Human
Human
Watt, P. , Mullen, Y. , Benhamou, P. , Hober, C. , Nomura, Y. , Watanabe, Y. , Passaro, E. , Zinner, M. and Brunicardi, F.
Simplified Semiautomated Method For Isolating Islets From the Human Pancreas ,
Transplant Proc
 26 (2) , 582 , 1994
Cells
Islets
Enzymes
Trypsin : Trypsin: 0.125-0.25%
Collagenase : 0.2%
Deoxyribonuclease I : 200 u/ml
Medium
Eurocollins solution
Pancreas
Human
Human, adult organ donors
Warnock, G. , Rajotte, R. , Evans, M. , Ellis, D. , DeGroot, T. and Dawidson, I.
Isolation of Islets of Langerhans Following Cold Storage of Human Pancreas ,
Transplant Proc
 XIX (4) , 3466 , 1987
Cells
Islets
Enzymes
Collagenase : 0.6%
Medium
Eurocollins solution
Pancreas
Human
Human
Izumi, R. , Konishi, K. , Ueno, K. , Shimizu, K. , Hirosawa, H. , Takahashi, N. and Miyazaki, I.
Isolation of Human Pancreatic Islets from Cryopreserved Pancreas ,
Transplant Proc
 XVII , 383 , 1985
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Human
Human
Gray, D. , McShane, P. , Grant, A. and Morris, P.
A Method for Isolation of Islets of Langerhans from the Human Pancreas ,
Diabetes
 33 , 1055 , 1984
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Human
Human, infant, age 1 day-1 year
Sutherland, D. , Matas, A. , Steffes, M. and Najarian, J.
Infant Human Pancreas: A Potential Source of Islet Tissue for Transplantation ,
Diabetes
 25 (12) , 1123 , 1976
Cells
Islets
Enzymes
Collagenase : 170-210 u/ml
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.