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Tissue References Search Tool

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Epithelial
Human
Human
Gottipamula, S. , Saraswat, S. and Sridhar, K.
Comparative Study of Isolation, Expansion and Characterization of Epithelial Cells. ,
Cytotherapy
 19 , 263-271 , 2017
Cells
Epithelial
Enzymes
Medium
PBS
Epithelial
Human
Human
Hsueh, Y. , Huang, S. , Lai, J. , Ma, S. , Chen, H. , Wu, S. , Wang, T. , Sun, C. , Ma, K. , Chen, J. , Lai, C. and Ma, D.
Preservation of Epithelial Progenitor Cells from Collagenase-Digested Oral Mucosa During Ex Vivo Cultivation. ,
Sci Rep
 6 , 36266 , 2016
Cells
Oral mucosal epithelial
Enzymes
Medium
DMEM/F12
Epithelial
Human
Human
VanDussen, K. , Marinshaw, J. , Shaikh, N. , Miyoshi, H. , Moon, C. , Tarr, P. , Ciorba, M. and Stappenbeck, T.
Development of an Enhanced Human Gastrointestinal Epithelial Culture System to Facilitate Patient-Based Assays. ,
Gut
 64 , 911-20 , 2015
Cells
Gastrointestinal epithelial
Enzymes
Medium
DMEM/F12
Epithelial
Human
Human
Widdicombe JH, Sachs LA, Morrow JL. and Finkbeiner WE.
Expansion of cultures of human tracheal epithelium with maintenance of differentiated structure and function ,
Biotechniques
 39(2) , 249 , 2005
Cells
Human tracheal epithelium
Enzymes
Protease Type XIV : 0.04%
Medium
DMEM/F12
Epithelial
Human
Human
Fukushima, K. and Fiocchi, C.
Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients. ,
Am J Physiol Gastrointest Liver Physiol
 286 , G804-13 , 2004
Cells
Colonic epithelial
Enzymes
Medium
RPMI 1640
Epithelial
Human
Human
Espana EM, Romano AC, Kawakita T, Di Pascuale M, Smiddy R. and Tseng SC
Novel enzymatic isolation of an entire viable human limbal epithelial sheet ,
Inv Ophthal Visual Sci
 44(10) , 4275 , 2003
Cells
Corneal limbal epithelial sheet
Enzymes
Medium
see reference
Epithelial
Human
Human
Fahlgren, A. , Hammarstrom, S. , Danielsson, A. and Hammarstrom, M.
Increased Expression of Antimicrobial Peptides and Lysozyme in Colonic Epithelial Cells of Patients with Ulcerative Colitis. ,
Clin Exp Immunol
 131 , 90 , 2003
Cells
Intestinal epithelial
Enzymes
Collagenase Type 4 : 72.5 u/ml
Medium
HBSS
Epithelial
Human
Human
Smoot, D. , Sewchand, J. , Young, K. , Desbrodes, B. , Allen, C. and Naab, T.
A Method for Establishing Primary Cultures of Human Gastric Epithelial Cells ,
Meth Cell Sci
 22 , 133 , 2000
Cells
Gastric epithelial cells
Enzymes
Medium
L-15
Epithelial
Human
Human
Halbert CL, Alexander IE, Wolgamot GM, Miller AD.
Adeno-associated virus vectors transduce primary cells much less efficiently than immortalized cells ,
J Virol
 69 , 1473-9 , 1995
Cells
Nasal polyp epithelial
Enzymes
Trypsin : 0.1%
Medium
see reference
Epithelial
Human
Human
Sarosiek, J, Marshall, B, Peura, D, Guerrant, L, McCallum, R. and Little, C
The Isolation and Maintenance of Human Gastric Epithelial Cells in Primary Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:10.1 , 1995
Cells
Gastric
Enzymes
Medium
F-12 medium
Epithelial
Human
Human
Sabatini, L. , Allen-Hoffmann, B, Warner, T. and Azen, E.
Serial Cultivation of Epithelial Cells from Human and Macaque Salivary Glands ,
In Vitro Cell Dev Biol
 27A , 939 , 1991
Cells
Epithelial
Enzymes
Trypsin : 0.05%
Medium
DMEM
Epithelial
Human
Human
Robinson, C. and Wu, R.
Culture of Conducting Airway Epithelial Cells in Serum-Free Medium ,
J Tiss Cul Meth
 13 , 95 , 1991
Cells
Epithelial
Enzymes
Trypsin : 0.2%
Medium
MEM, PBS
Epithelial
Human
Human
Emerman, J. and Wilkinson, D.
Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples ,
In Vitro Cell Dev Biol
 26 , 1186 , 1990
Cells
Epithelial
Enzymes
Collagenase : 2.0%
Medium
DMEM/Ham's F-12
Epithelial
Human
Human
Gruenert, D.C. , Basbaum, C.B. and Widdicombe, J.H.
Long-Term Culture of Normal and Cystic Fibrosis Epithelial Cells Grown Under Serum-Free Conditions ,
In Vitro Cell Dev Biol
 26 , 411 , 1990
Cells
Epithelial
Enzymes
Pronase : 0.1%
Medium
PBS
Epithelial
Human
Human
Wood, L. and Neufeld, E.
A Cystic Fibrosis Phenotype in Cells Cultured from Sweat Gland Secretory Coil. Altered Kinetics of 36Cl Efflux ,
J Biol Chem
 265 , 12796 , 1990
Cells
Epithelial
Sweat gland
Enzymes
Medium
(see reference)
Epithelial
Human
Human
(also bovine)
Munson, L. , Chandler, S. and Schlafer, D.
Cultivation of Bovine Fetal and Adult Endometrial Epithelial Cells ,
J Tiss Cul Meth
 11 (3) , 129 , 1988
Cells
Endometrial epithelial
Medium
DMEM/Ham's F-12
Epithelial
Human
Human
Widdicombe, J.H. , Coleman, D.L. , Finkbeiner, W.E. and Tuet, I.K.
Electrical Properties of Monolayers Cultured From Cells of Human Tracheal Mucosa ,
J Appl Physiol
 58 , 1729 , 1985
Cells
Epithelial
Enzymes
Medium
HEPES with 5.9mM Glucose,5mM DTT
Epithelial
Human
Human
Yankaskas, J. , Cotton, C. , Knowles, M. , Gatzy, J. and Boucher, R.
Culture of Human Nasal Epithelial Cells on Collagen Matrix Supports ,
Am Rev Respir Dis
 132 , 1281 , 1985
Cells
Epithelial
Enzymes
Protease Type XIV : 0.1%
Medium
Eagle's MEM
Epithelial
Human
Human, women, 27-49 years
Auersperg, N. , Siemens, C.H. and Myrdal, S.E.
Human Ovarian Surface Epithelium In Primary Culture ,
In Vitro
 20 , 743 , 1984
Cells
Epithelial
Ovary
Enzymes
Trypsin : 0.125%
Medium
HBSS, CMF
Epithelial
Human
Human, infant and neonate
Lechner, J. , Babcock, M. , Marnell, M. , Narayan, K. and Kaighn, M.
Normal Human Prostate Epithelial Cell Cultures ,
Methods Cell Biol
 21 , 195 , 1980
Cells
Epithelial
Prostate
Enzymes
Trypsin : 0.1%
Medium
HBSS
Organoids
Human

Human

Gamero-Estevez, E. , Hensel, I. , Steinhauer, M. , Müllertz, O. , Savochkina, E. , Sektioglu, I. , Stoll, B. , Derakhshani, S. , Devriese, S. , Kim, K. and Resnik-Docampo, M.
Protocol for Immune Cell Isolation, Organoid Generation, and Co-culture Establishment from Cryopreserved Whole Human Intestine. ,
Bio Protoc
 15 , e5157 , 2025
Cells
Intestinal organoids
Enzymes
Medium
RPMI
Organoids
Human

Human

Geest, A. , Jakobs, C. , Ljubikj, T. , Huffels, C. , Luna, M. , Sá, R. , Adolfs, Y. , Wit, M. , Rutten, D. , Kaal, M. , Zwartkruis, M. , Carcolé, M. , Groen, E. , Hol, E. , Basak, O. , Isaacs, A. , Westeneng, H. , Berg, L. , Veldink, J. , Schlegel, D. and Pasterkamp, R.
Molecular Pathology, Developmental Changes, and Synaptic Dysfunction in (pre-) Symptomatic Human C9ORF72-ALS/FTD Cerebral Organoids. , Acta Neuropathol Commun Vol. 12 , 152 , 2024
Cells
Cerebral organoids
Enzymes
Papain : 20 u/ml
Medium
DMEM/F12
Organoids
Human

Human

Lisowski, P. , Lickfett, S. , Rybak-Wolf, A. , Menacho, C. and Le, S.
Mutant Huntingtin Impairs Neurodevelopment in Human Brain Organoids through CHCHD2-mediated Neurometabolic Failure. ,
Nat Commun
 15 , 7027 , 2024
Cells
Brain organoids
Enzymes
Papain : 20 u/ml
Medium
EBSS
Organoids
Human

Human

Tresenrider, A. , Sridhar, A. , Eldred, K. , Cuschieri, S. , Hoffer, D. , Trapnell, C. and Reh, T.
Single-cell Sequencing of Individual Retinal Organoids Reveals Determinants of Cell Fate Heterogeneity. , bioRxiv , 2023
Cells
Retinal Organoids
Enzymes
PDS kit : see reference
Medium
DMEM
Organoids
Human

Human

Chiu, M. , Li, C. , Yu, Y. , Liu, X. , Huang, J. , Wan, Z. , Yuen, K. and Zhou, J.
Establishing Bipotential Human Lung Organoid Culture System and Differentiation to Generate Mature Alveolar and Airway Organoids. ,
Bio Protoc
 13 , e4657 , 2023
Cells
Lung Organoids
Enzymes
Medium
Basal medium
Organoids
Human

Human, adolescent

Hemeryck, L. , Hermans, F. , Chappell, J. , Kobayashi, H. , Lambrechts, D. , Lambrichts, I. , Bronckaers, A. and Vankelecom, H.
Organoids from Human Tooth Showing Epithelial Stemness Phenotype and Differentiation Potential. ,
Cell Mol Life Sci
 79 , 153 , 2022
Cells
Epithelial organoids
Enzymes
Medium
MEM
Organoids
Human

Human

Schröter, M. , Wang, C. , Terrigno, M. , Hornauer, P. , Huang, Z. , Jagasia, R. and Hierlemann, A.
Functional Imaging of Brain Organoids Using High-Density Microelectrode Arrays. , MRS Bull Vol. 47 , 530-544 , 2022
Cells
Cerebral organoids
Enzymes
PDS kit : see reference
Medium
PBS
Organoids
Human

Human

Uzquiano, A. , Kedaigle, A. , Pigoni, M. , Paulsen, B. , Adiconis, X. , Kim, K. , Faits, T. , Nagaraja, S. , Antón-Bolaños, N. , Gerhardinger, C. , Tucewicz, A. , Murray, E. , Jin, X. , Buenrostro, J. , Chen, F. , Velasco, S. , Regev, A. , Levin, J. and Arlotta, P.
Proper Acquisition of Cell Class Identity in Organoids Allows Definition of Fate Specification Programs of the Human Cerebral Cortex ,
Cell
 185 , 3770-3788.e27 , 2022
Cells
Brain organoids
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Xue, Y. , Seiler, M. , Tang, W. , Wang, J. , Delgado, J. , McLelland, B. , Nistor, G. , Keirstead, H. and Browne, A.
Retinal Organoids On-a-Chip: a Micro-Millifluidic Bioreactor for Long-term Organoid Maintenance. ,
Lab Chip
 21 , 3361-3377 , 2021
Cells
Stem cell-derived retinal organoids
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Vaart, J. , Böttinger, L. , Geurts, M. , Wetering, W. , Knoops, K. , Sachs, N. , Begthel, H. , Korving, J. , Lopez-Iglesias, C. , Peters, P. , Eitan, K. , Gileles-Hillel, A. and Clevers, H.
Modelling of Primary Ciliary Dyskinesia using Patient-derived Airway Organoids. , EMBO Rep Vol. 22 , e52058 , 2021
Cells
Airway organoids
Enzymes
Pronase : 0.15%
Collagenase : 0.05%
Medium
Airway medium
Organoids
Human

Human

Marinucci, M. , Ercan, C. , Taha-Mehlitz, S. , Fourie, L. , Panebianco, F. , Bianco, G. , Gallon, J. , Staubli, S. , Soysal, S. , Zettl, A. , Rauthe, S. , Vosbeck, J. , Droeser, R. , Bolli, M. , Peterli, R. , Flüe, M. , Ng, C. , Kollmar, O. , Coto-Llerena, M. and Piscuoglio, S.
Standardizing Patient-Derived Organoid Generation Workflow to Avoid Microbial Contamination From Colorectal Cancer Tissues. , Front Oncol Vol. 11 , 781833 , 2021
Cells
Colorectal cancer organoids
Enzymes
Medium
DMEM/F-12
Organoids
Human

Human

Schreurs, R. , Baumdick, M. , Drewniak, A. and Bunders, M.
Co-culture of Human Intestinal Organoids and Lamina Propria-derived CD4 T Cells. , STAR Protoc Vol. 2 , 100519 , 2021
Cells
Lamina propria lymphocytes
Enzymes
Medium
DMEM/F-12
Organoids
Human

Human

Barbar, L. , Rusielewicz, T. , Zimmer, M. , Kalpana, K. and Fossati, V.
Isolation of Human CD49f Astrocytes and iPSC-Based Neurotoxicity Assays. , STAR Protoc Vol. 1 , 100172 , 2020
Cells
Astrocytes
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Velasco, S. , Kedaigle, A. , Simmons, S. , Nash, A. , Rocha, M. , Quadrato, G. , Paulsen, B. , Nguyen, L. , Adiconis, X. , Regev, A. , Levin, J. and Arlotta, P.
Individual Brain Organoids Reproducibly Form Cell Diversity of the Human Cerebral Cortex. ,
Nature
 570 , 523-527 , 2019
Cells
Brain organoids
Enzymes
PDS kit : see reference
Medium
PBS
Organoids
Human

Human

Mansour, A. , Gonçalves, J. , Bloyd, C. , Li, H. , Fernandes, S. , Quang, D. , Johnston, S. , Parylak, S. , Jin, X. and Gage, F.
An In Vivo Model of Functional and Vascularized Human Brain Organoids. ,
Nat Biotechnol
 36 , 432-441 , 2018
Cells
Cerebral organoids
Enzymes
Medium
DMEM/F12
Organoids
Human

Human, embryonic

Nikolić, M. , Caritg, O. , Jeng, Q. , Johnson, J. , Sun, D. , Howell, K. , Brady, J. , Laresgoiti, U. , Allen, G. , Butler, R. , Zilbauer, M. , Giangreco, A. and Rawlins, E.
Human Embryonic Lung Epithelial Tips are Multipotent Progenitors that can be Expanded In vitro as Long-term Self-renewing Organoids. ,
Elife
 6 , 2017
Cells
Lung organoids
Enzymes
Medium
DMEM/F12

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.