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Tissue References Search Tool

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Eye
Human
Human
Yusoff,B, Riau, A. , Yam, G. , Halim, N. and Mehta, J.
Isolation and Propagation of Human Corneal Stromal Keratocytes for Tissue Engineering and Cell Therapy. ,
Cells
 11 , 178 , 2022
Cells
Corneal stromal keratocytes
Enzymes
Medium
DMEM/F-12
Eye
Human

Human

Menon, M. , Mohammadi, S. , Davila-Velderrain, J. , Goods, B. , Cadwell, T. , Xing, Y. , Stemmer-Rachamimov, A. , Shalek, A. , Love, J. , Kellis, M. and Hafler, B.
Single-cell Transcriptomic Atlas of the Human Retina Identifies Cell Types Associated with Age-related Macular Degeneration. ,
Nat Commun
 10 , 4902 , 2019
Cells
Retinal
Enzymes
PDS kit : see reference
Medium
EBSS
Eye
Human
Human
Xu, N. , Chen, Y. , Dean, K. , Lu, X. , Liu, X. , Wang, W. , Dean, D. , Kaplan, H. , Gao, L. , Dong, F. and Liu, Y.
Sphere-Induced Rejuvenation of Swine and Human Muller Glia Is Primarily Caused by Telomere Elongation. ,
Stem Cells
 35 , 1579-1591 , 2017
Cells
Muller glia
Enzymes
PDS kit : per instructions
Medium
DMEM
Eye
Human
Human
Sidney, L. , Branch, M. , Dua, H. and Hopkinson, A.
Effect of Culture Medium on Propagation and Phenotype of Corneal Stroma-Derived Stem Cells. ,
Cytotherapy
 17 , 1706-22 , 2015
Cells
Corneal stroma stem
Enzymes
Medium
DMEM
Eye
Human
Human
Du, Y. , Roh, D. , Funderburgh, M. , Mann, M. , Marra, K. , Rubin, J. , Li, X. and Funderburgh, J.
Adipose-Derived Stem Cells Differentiate to Keratocytes In Vitro. ,
Mol Vis
 16 , 2680 , 2010
Cells
Corneal stromal stem
Enzymes
Neutral Protease : 1.2 u/ml
Collagenase : 0.1%
Medium
DMEM
Eye
Human
Human, 18-68 yr
Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G.
A novel method of isolation, preservation, and expansion of human corneal endothelial cells ,
Inv Ophthal Visual Sci
 48 , 614-20 , 2007
Cells
Corneal endothelial
Enzymes
Medium
DMEM/F12
Eye
Human
Human
Espana EM, Romano AC, Kawakita T, Di Pascuale M, Smiddy R. and Tseng SC
Novel enzymatic isolation of an entire viable human limbal epithelial sheet ,
Inv Ophthal Visual Sci
 44(10) , 4275 , 2003
Cells
Corneal limbal epithelial sheet
Enzymes
Medium
see reference
Eye
Human
Human, 5-65 years
Von Recum, H. , Okano, T. , Kim, S. and Bernstein, P.
Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture ,
Exp Eye Res
 69 , 97 , 1999
Cells
Retinal pigment epithelial (RPE)
Enzymes
Trypsin : 0.25%
Medium
HBSS
Kidney
Human
Human
Basu, J. , Genheimer, C. , Sangha, N. , Quinlan, S. , Guthrie, K. , Kelley, R. , Ilagan, R. , Jain, D. , Bertram, T. and Ludlow, J.
Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. ,
Lipids Health Dis
 10 , 171 , 2011
Cells
Renal adipose derived cells
Enzymes
Medium
DMEM
Kidney
Human
Human, adult
Valente, M. , Henrique, R. , Costa, V. , Jeronimo, C. , Carvalho, F. , Bastos, M. , Guedes de Pinho, P. and Carvalho, M.
A Rapid and Simple Procedure for the Establishment of Human Normal and Cancer Renal Primary Cell Cultures from Surgical Specimens. ,
PLoS ONE
 6 , e19337 , 2011
Cells
Renal tumor cells and proximal tubular epithelial
Enzymes
Medium
DMEM/F12
Kidney
Human
Human
Presnell, S. , Bruce, A. , Wallace, S. , Choudhury, S. , Genheimer, C. , Cox, B. , Guthrie, K. , Werdin, E. , Tatsumi-Ficht, P. , Ilagan, R. , Kelley, R. , Rivera, E. , Ludlow, J. , Wagner, B. , Jayo, M. and Bertram, T.
Isolation, Characterization, and Expansion Methods for Defined Primary Renal Cell Populations from Rodent, Canine, and Human Normal and Diseased Kidneys. ,
Tiss Eng
 17 , 261 , 2011
Cells
Renal cells
Enzymes
Collagenase Type 4 : 300 u/ml
Neutral Protease : see reference
Medium
HBSS
Kidney
Human
Human, adult
Johnson, D. , Saunders, H. , Johnson, F. , Huq, S. , Field, M. and Pollock, C.
Cyclosporin exerts a direct fibrogenic effect on human tubulointerstitial cells: roles of insulin-like growth factor I, transforming growth factor beta1, and platelet-derived growth factor ,
J Pharmacol Exp Ther
 289 , 535-42 , 1999
Cells
Renal proximal tubule and cortical fibroblasts
Enzymes
Collagenase Type 2 : 383 u/ml
Medium
DMEM/F-12
Kidney
Human
Human
McAteer, J, Kempson, S. and Evan, A
Culture of Human Renal Cortex Epithelial Cells ,
J Tiss Cul Meth
 13 , 143 , 1991
Cells
Renal cortex
Enzymes
Trypsin : 0.1%
Medium
Tissue Culture Grade Water
Kidney
Human
Human
Trifillis, A. and Kahng, M.
Characterization of an In Vitro System of Human Renal Papillary Collecting Duct Cells ,
In Vitro Cell Dev Biol
 26 , 441 , 1990
Cells
Papillary duct
Enzymes
Collagenase : 400 u/ml
Medium
Eagle's MEM-HEPES buffer w/L-glutamine
Kidney
Human
Human
Heieren, M. , van der Woude, F. and Balfour Jr., H.
Cytomegalovirus Replicates Efficiently in Human Kidney Mesangial Cells ,
Proc Natl Acad Sci U S A
 85 , 1642 , 1988
Cells
Mesangial
Enzymes
Trypsin : 0.25%
Medium
DMEM/Ham's F-12
Kidney
Human
Human, adult
Yang, A. , Gould-Kostka, J. and Oberley, T.
In Vitro Growth and Differentiation of Human Kidney Tubular Cells on a Basement Membrane Substrate ,
In Vitro Cell Dev Biol
 23 (1) , 34 , 1987
Cells
Tubular
Enzymes
Collagenase : 250 u/ml
Medium
PBS
Kidney
Human
Human, adult, 14-66 years
Trifillis, A. , Regec, A. and Trump, B.
Isolation, Culture, and Charaterization of Human Renal Tubular Cells ,
J Urol
 133 , 324 , 1985
Cells
Tubular
Enzymes
Collagenase : 100 u/ml
Medium
Joklik's MEM
Kidney
Human
Human
Hemstreet, G. , Enoch, P. and Pretlow, T.
Tissue Disaggregation of Human Renal Cell Carcinoma with Further Isopyknic and Isokinetic Gradient Purification ,
Cancer Res
 40 , 1043 , 1980
Cells
Malignant
Stromal
Enzymes
Papain : 0.009%
Medium
Sacks solution
Kidney
Human
Human, 24 newborn and stillborn
(also rabbit)
De Oca, H. and Malinin, T.
Dispersion and Cultivation of Renal Cells After Short-Term Storage of Kidneys ,
J Clin Microbiol
 2 , 243 , 1975
Cells
Renal
Enzymes
Trypsin : 0.25%
Medium
See reference

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.