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Tissue References Search Tool

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Eye
Human
Human
Yusoff,B, Riau, A. , Yam, G. , Halim, N. and Mehta, J.
Isolation and Propagation of Human Corneal Stromal Keratocytes for Tissue Engineering and Cell Therapy. ,
Cells
 11 , 178 , 2022
Cells
Corneal stromal keratocytes
Enzymes
Medium
DMEM/F-12
Eye
Human

Human

Menon, M. , Mohammadi, S. , Davila-Velderrain, J. , Goods, B. , Cadwell, T. , Xing, Y. , Stemmer-Rachamimov, A. , Shalek, A. , Love, J. , Kellis, M. and Hafler, B.
Single-cell Transcriptomic Atlas of the Human Retina Identifies Cell Types Associated with Age-related Macular Degeneration. ,
Nat Commun
 10 , 4902 , 2019
Cells
Retinal
Enzymes
PDS kit : see reference
Medium
EBSS
Eye
Human
Human
Xu, N. , Chen, Y. , Dean, K. , Lu, X. , Liu, X. , Wang, W. , Dean, D. , Kaplan, H. , Gao, L. , Dong, F. and Liu, Y.
Sphere-Induced Rejuvenation of Swine and Human Muller Glia Is Primarily Caused by Telomere Elongation. ,
Stem Cells
 35 , 1579-1591 , 2017
Cells
Muller glia
Enzymes
PDS kit : per instructions
Medium
DMEM
Eye
Human
Human
Sidney, L. , Branch, M. , Dua, H. and Hopkinson, A.
Effect of Culture Medium on Propagation and Phenotype of Corneal Stroma-Derived Stem Cells. ,
Cytotherapy
 17 , 1706-22 , 2015
Cells
Corneal stroma stem
Enzymes
Medium
DMEM
Eye
Human
Human
Du, Y. , Roh, D. , Funderburgh, M. , Mann, M. , Marra, K. , Rubin, J. , Li, X. and Funderburgh, J.
Adipose-Derived Stem Cells Differentiate to Keratocytes In Vitro. ,
Mol Vis
 16 , 2680 , 2010
Cells
Corneal stromal stem
Enzymes
Neutral Protease : 1.2 u/ml
Collagenase : 0.1%
Medium
DMEM
Eye
Human
Human, 18-68 yr
Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G.
A novel method of isolation, preservation, and expansion of human corneal endothelial cells ,
Inv Ophthal Visual Sci
 48 , 614-20 , 2007
Cells
Corneal endothelial
Enzymes
Medium
DMEM/F12
Eye
Human
Human
Espana EM, Romano AC, Kawakita T, Di Pascuale M, Smiddy R. and Tseng SC
Novel enzymatic isolation of an entire viable human limbal epithelial sheet ,
Inv Ophthal Visual Sci
 44(10) , 4275 , 2003
Cells
Corneal limbal epithelial sheet
Enzymes
Medium
see reference
Eye
Human
Human, 5-65 years
Von Recum, H. , Okano, T. , Kim, S. and Bernstein, P.
Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture ,
Exp Eye Res
 69 , 97 , 1999
Cells
Retinal pigment epithelial (RPE)
Enzymes
Trypsin : 0.25%
Medium
HBSS
Tumor
Human

Human

Song, H. , Weinstein, H. , Allegakoen, P. , Wadsworth, M. , Xie, J. , Yang, H. , Castro, E. , Lu, K. , Stohr, B. , Feng, F. , Carroll, P. , Wang, B. , Cooperberg, M. , Shalek, A. and Huang, F.
Single-cell Analysis of Human Primary Prostate Cancer Reveals the Heterogeneity of Tumor-associated Epithelial Cell States. ,
Nat Commun
 13 , 141 , 2022
Cells
Prostate cells
Enzymes
Collagenase Type 4 : 1,000 u/ml
Medium
RPMI
Tumor
Human
Human
Jeske, S. , Schuler, P. , Doescher, J. , Theodoraki, M. , Laban, S. , Brunner, C. , Hoffman, T. and Wigand, M.
Age-related changes in T lymphocytes of patients with head and neck squamous cell carcinoma ,
Immun Ageing
 17 , 3 , 2020
Cells
Tumor infiltrating lympocytes
Enzymes
Collagenase Type 1 : 200 u/ml
Medium
RPMI
Tumor
Human
Human
Ayuso, J. , Vitek, R. , Swick, A. , Skala, M. , Wisinski, K. , Kimple, R. , Lambert, P. and Beebe, D.
Effects of Culture Method on Response to EGFR Therapy in Head and Neck Squamous Cell Carcinoma Cells ,
Sci Rep
 9 , 12480 , 2019
Cells
Fibroblasts
Enzymes
Medium
FM Fibroblast media
Tumor
Human
Human
Phan, N. , Hong, J. , Tofig, B. , Mapua, M. , Elashoff, D. , Moatamed, N. , Huang, J. , Memarzadeh, S. , Damoiseaux, R. and Soragni, A.
A Simple High-Throughput Approach Identifies Actionable Drug Sensitivities in Patient-Derived Tumor Organoids. ,
Commun Biol
 2 , 78 , 2019
Cells
Tumor
Enzymes
Collagenase Type 4 : 200 u/ml
Medium
DMEM
Tumor
Human
Neal, J,, Li ,X. , Zhu, J. , Giangarra, V. , Grzeskowiak, C. , Ju, J. , Liu, I. Chiou, S. , Salahudeen, A. and Smith, A. et al
Organoid Modeling of the Tumor Immune Microenvironment. ,
Cell
 175 , 1972 , 2018
Cells
Organoids
Enzymes
Collagenase Type 4 : 200-300 u/ml
Medium
DMEM
Tumor
Human
Human
Jiang, C. , Zhang, Q. , Shanti, R. , Shi, S. , Chang, T. , Carrasco, L. , Alawi, F. and Le, A.
Mesenchymal Stromal Cell-Derived Interleukin-6 Promotes Epithelial-Mesenchymal Transition and Acquisition of Epithelial Stem-Like Cell Properties in Ameloblastoma Epithelial Cells. ,
Stem Cells
 35 , 2083-2094 , 2017
Cells
Ameloblastoma epithelial
Enzymes
Collagenase : 0.1%
Neutral Protease : 50 u/ml
Medium
MEM
Tumor
Human
Human, 25-81 yr
Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,
A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
 17 , 30 , 2016
Cells
Brain tumor
Enzymes
Neutral Protease : 0.11 u/ml
Hyaluronidase : 1,000 u/ml
Medium
HBSS
Tumor
Human
Human
Pham, K. , Delitto, D. , Knowlton, A. , Hartlage, E. , Madhavan, R. , Gonzalo, D. , Thomas, R. , Behrns, K. , George, T. , Hughes, S. , Wallet, S. , Liu, C. and Trevino, J.
Isolation of Pancreatic Cancer Cells from a Patient-Derived Xenograft Model Allows for Practical Expansion and Preserved Heterogeneity in Culture. ,
Am J Pathol
 186 , 2016
Cells
Pancreatic cancer
Enzymes
Stemxyme 1 : 0.2%
Medium
HBSS
Tumor
Human
Human
Baldan, V. , Griffiths, R. , Hawkins, R. and Gilham, D
Efficient and Reproducible Generation of Tumour-Infiltrating Lymphocytes for Renal Cell Carcinoma ,
Br J Cancer
 112 , 1510 , 2015
Cells
Tumour infiltrating lymphocytes
Enzymes
Medium
RPMI 1640
Tumor
Human
Human
Ali, M. , Anand, S. , Tangella, K. , Ramkumar, D. and Saif, T.
Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry. ,
J Vis Exp
 , e52532 , 2015
Cells
Colon
Enzymes
Trypsin : 0.1%
Collagenase : 0.1%
Medium
HBSS
Tumor
Human
Human
Liang, S. , Marti, T. , Dorn, P. , Froment, L. , Hall, S. , Berezowska, S. , Kocher, G. , Schmid, R. and Peng, R.
Blocking the Epithelial-to-Mesenchymal Transition Pathway Abrogates Resistance to Anti-Folate Chemotherapy in Lung Cancer. ,
Cell Death Dis
 6 , e1824 , 2015
Cells
Non-small cell lung tumor
Enzymes
Medium
RPMI-1640
Tumor
Human
Human
Quatromoni, J. , Singhal, S. , Bhojnagarwala, P. , Hancock, W. , Albelda, S. and Eruslanov, E.
An Optimized Dissagregation Method for Human Lung Tumors That Perserves the Phenotype and Function of the Immune Cells ,
J Leukoc Biol
 97 , 201 , 2015
Cells
Lung tumor
Enzymes
Collagenase Type 1 : 45-60 u/ml
Collagenase Type 2 : 15-20 u/ml
Collagenase Type 4 : 45-60 u/ml
Elastase : 0.002%
Medium
DMEM/F12
Tumor
Human
Human
Yan, K. , Wu, Q. , Yan, D. , Lee, C. , Rahim, N. , Tritschler, I. , DeVecchio, J. , Kalady, M. , Hjelmeland, A. and Rich, J.
Glioma Cancer Stem Cells Secrete Gremlin1 to Promote their Maintenance Within the Tumor Hierarchy. ,
Genes Dev
 28 , 1085-100 , 2014
Cells
Glioma cancer stem
Enzymes
PDS kit : per instructions
Medium
Neurobasal
Tumor
Human
Human
Hasselbach, L. , Irtenkauf, S. , Lemke, N. , Nelson, K. , Berezovsky, A. , Carlton, E. , Transou, A. , Mikkelsen, T. and deCarvalho, A.
Optimization of High Grade Glioma Cell Culture from Surgical Specimens for Use in Clinically Relevant Animal Models and 3D Immunochemistry. ,
J Vis Exp
 , e51088 , 2014
Cells
Glioblastoma tumor
Enzymes
Trypsin : 0.025%
Collagenase Type 4 : 500 u/ml
Medium
HBSS
Tumor
Human
Human
Dobbin, Z. and Landen, C.
Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors--Potential Applications. ,
Curr Protoc Pharmacol
 63 , Unit 14.28. , 2013
Cells
Cancer stem
Enzymes
Trypsin : 0.25%
Collagenase : 0.05%
Hyaluronidase : 0.005%
Medium
RPMI
Tumor
Human
Human
Chou, J. , Fitzgibbon, M. , Mortales, C. , Towlerton, A. , Upton, M. , Yeung, R. , McIntosh, M. and Warren, E.
Phenotypic and Transcriptional Fidelity of Patient-Derived Colon Cancer Xenografts in Immune-Deficient Mice. ,
PLoS ONE
 8 , e79874 , 2013
Cells
Tumor
Enzymes
Medium
DMEM/F12
Tumor
Human
Human
Welte, Y. , Davies, C. , Schafer, R. and Regenbrecht, C.
Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma. ,
J Vis Exp
 73 , e50200 , 2013
Cells
Melanoma
Enzymes
Medium
PBS
Tumor
Human
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
Tumor
Human
Human
Zhou, J, Belov, L. , Solomon, M. , Chan, C. , Clarke, S. and Christopherson, R.
Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing. ,
J Vis Exp
 55 , e3322 , 2011
Cells
Colorectal cancer
Enzymes
Medium
RPMI 1640
Tumor
Human
Human
Quintana, E. , Shackleton, M. , Foster, H. , Fullen, D. , Sabel, M. , Johnson, T. and Morrison, S.
Phenotypic Heterogeneity Among Tumorigenic Melanoma Cells from Patients that is Reversible and Not Hierarchically Organized. ,
Cancer Cell
 18 , 510 , 2010
Cells
Tumorigenic melanoma
Enzymes
Collagenase Type 4 : 200 u/ml
Trypsin : 0.05%
Deoxyribonuclease I : 50-100 u/ml
Medium
PBS
Tumor
Human
Human
Sauvageot, C. , Weatherbee, J. , Kesari, S. , Winters, S. , Barnes, J. , Dellagatta, J. , Ramakrishna, N. , Stiles, C. , Kung, A. , Kieran, M. and Wen, P.
Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells. , Neuro Oncol Vol. 11 , 109 , 2009
Cells
Tumor
Enzymes
Medium
see reference
Tumor
Human
Human
Varnat, F. , Duquet, A. , Malerba, M. , Zbinden, M. , Mas, C. , Gervaz, P. and Ruiz i Altaba, A.
Human Colon Cancer Epithelial Cells Harbour Active HEDGEHOG-GLI Signalling that is Essential for Tumour Growth, Recurrence, Metastasis and Stem Cell Survival and Expansion. ,
EMBO Mol Med
 1 , 338-51 , 2009
Cells
Colon cancer
Enzymes
Collagenase Type 1 : 300 u/ml
Hyaluronidase : 100 u/ml
Medium
DMEM/F12
Tumor
Human
Human
Kim, M. , Evans, D. , Wang, H. , Abbruzzese, J. , Fleming, J. and Gallick, G.
Generation of Orthotopic and Heterotopic Human Pancreatic Cancer Xenografts in Immunodeficient Mice. ,
Nat Protoc
 4 , 1670 , 2009
Cells
Pancreatic tumor
Enzymes
Collagenase Type 4 : 200 u/ml
Medium
RPMI-1640
Tumor
Human
Human
Liu, R. , Wang, X. , Chen, G. , Dalerba, P. , Gurney, A. , Hoey, T. , Sherlock, G. , Lewicki, J. , Shedden, K. and Clarke, M.
The Prognostic Role of a Gene Signature from Tumorigenic Breast-Cancer Cells. ,
N Engl J Med
 356 , 217 , 2007
Cells
Breast epithelial
Enzymes
Collagenase Type 3 : 200 u/ml
Medium
HBSS
Tumor
Human
Human
Nakashiro Koh-Ichi, Hara Shingo, Shinohara Yuji, Oyasu Miho, Kawamata Hitoshi, Shintani Satoru, Hamakawa Hiroyuki, Oyasu Ryoichi.
Phenotypic switch from paracrine to autocrine role of hepatocyte growth factor in an androgen-independent human prostatic carcinoma cell line, CWR22R ,
Am J Pathol
 165 , 533-40 , 2004
Cells
Prostate stromal cells
Enzymes
Medium
RPMI 1640
Tumor
Human
Human, adult
Nishio Jun, Iwasaki Hiroshi, Ishiguro Masko, Ohjimi Yuko, Fujita Chikako, Isayama Teruto, Naito Masatoshi, Oda Yoshinao, Kaneko Yasuhiko, Kikuchi Masahiro.
Establishment of a new human synovial sarcoma cell line, FU-SY-1, that expresses c-Met receptor and its ligand hepatocyte growth factor ,
Int J Oncol
 21 , 17-23 , 2002
Cells
Human synovial sarcoma
Enzymes
Collagenase Type 2 : 200 u/ml
Medium
DMEM/F-12
Tumor
Human
Human
Emenaker N, Calaf G, Cox D, Basson M. and Qureshi N
Short Chain Fatty Acids Differentially Modulate Cellular Phenotype and c-myc Protein Levels in Primary Human Nonmalignant and Malignant Colonocytes ,
J Nutr
 46 , 96-105 , 2001
Cells
Colonocytes
Enzymes
Collagenase : 0.15%
Medium
DMEM/F12
Tumor
Human
Human
MacLeod, R.
Rapid Monolayer Primary Cell Culture from Tissue Biopsy , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons Ltd , 3E:2.1 , 1995
Cells
Epithelial, fibroblasts
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.