For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.

Quick Order

Tissue References Search Tool

Search
Heart
Human
Human
Holt-Casper, D. , Theisen, J. , Moreno, A. , Warren, M. , Silva, F. , Grainger, D. , Bull, D. and Patel, A.
Novel Xeno-free Human Heart Matrix-Derived Three-Dimensional Scaffolds. ,
J Transl Med
 13 , 194 , 2015
Cells
Cardiac
Enzymes
Collagenase : 0.045%
Pancreatin : 0.1%
Medium
DPBS
Heart
Human
Human
Sharma, S. , Mishra, R. , Simpson, D. , Wehman, B. , Colletti, E. , Deshmukh, S. , Datla, S. , Balachandran, K. , Guo, Y. , Chen, L. , Siddiqui, O. , Kaushal, S. and Kaushal, S.
Cardiosphere-Derived Cells From Pediatric End-Stage Heart Failure Patients Have Enhanced Functional Activity Due to the Heat Shock Response Regulating the Secretome. ,
Stem Cells
 33 , 1213-29 , 2015
Cells
Cardiospheres
Enzymes
Medium
Iscove modified DMEM
Heart
Human
Human
Avolio, E. , Gianfranceschi, G. , Cesselli, D. , Caragnano, A. , Athanasakis, E. , Katare, R. , Meloni, M. , Palma, A. , Barchiesi, A. , Vascotto, C. , Toffoletto, B. , Mazzega, E. , Finato, N. , Aresu, G. and Livi, U.
Ex vivo Molecular Rejuvenation Improves the Therapeutic Activity of Senescent Human Cardiac Stem Cells in a Mouse Model of Myocardial Infarction. ,
Stem Cells
 32 , 2373 , 2014
Cells
Cardiac stem
Enzymes
Medium
Joklik modified Eagle’s
Heart
Human
Human
Voigt, N. , Zhou, X. and Dobrev, D.
Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents. ,
J Vis Exp
 , e50235 , 2013
Cells
Atrial myocytes
Enzymes
Collagenase Type 1 : 286 u/ml
Protease : 5 u/ml
Medium
see references
Heart
Human
Human
Vukusic, K. , Jonsson, M. , Brantsing, C. , Dellgren, G. , Jeppsson, A. , Lindahl, A. and Asp, J.
High Density Sphere Culture of Adult Cardiac Cells Increases the Levels of Cardiac and Progenitor Markers and Shows Signs of Vasculogenesis. ,
Biomed Res Int
 2013 , 2013
Cells
Cardiac
Enzymes
Medium
DMEM
Heart
Human
Human
Anderson, E. , Rodriguez, E. , Anderson, C. , Thayne, K. , Chitwood, W. and Kypson, A.
Increased Propensity for Cell Death in Diabetic Human Heart is Mediated by Mitochondrial-Dependent Pathways. ,
Am J Physiol Heart Circ Physiol
 300 , H118 , 2011
Cells
Myofibers
Enzymes
Medium
see reference
Heart
Human
Human
Voigt, N. , Makary, S. , Nattel, S. and Dobrev, D.
Voltage-Clamp-Based Methods for the Detection of Constitutively Active Acetylcholine-Gated I(K,ACh) Channels in the Diseased Heart. ,
Methods Enzymol
 484 , 653 , 2010
Cells
Atrial cardiomyocytes
Enzymes
Collagenase Type 1 : 286 u/ml
Protease : 5 u/ml
Medium
see reference
Heart
Human
Human
Jensen, B. , Swigart, P. , Laden, M. , DeMarco, T. , Hoopes, C. and Simpson, P.
The Alpha-1D Is the Predominant Alpha-1-Adrenergic Receptor Subtype in Human Epicardial Coronary Arteries. ,
J Am Coll Cardiol
 54 , 1137 , 2009
Cells
Coronary artery smooth muscle
Enzymes
Elastase : 0.05%
Medium
DMEM
Heart
Human
Human
Mathew, S. , Tustison, K. , Sugatani, T. , Chaudhary, L. , Rifas, L. and Hruska, K.
The Mechanism of Phosphorus as a Cardiovascular Risk Factor in CKD. ,
J Am Soc Nephrol
 19 , 1092 , 2008
Cells
Smooth muscle aortic
Enzymes
Medium
DMEM
Heart
Human
Human (n=16, age: 60 &plusmin;3 years)
Mukerjee, R. , Multani, M. , Sample, J. , Dowdy, K. , Zellner, J. , Hoover, D. and Spinale, F.
Effects of adrenomedullin on human myocyte contractile function and beta-adrenergic response ,
J Cardiovasc Pharmacol
 7 (4) , 235 , 2002
Cells
Myocardial
Enzymes
Hyaluronidase : 0.05%
Medium
Medium 199
Heart
Human
Human
Todor Anastassia, Sharov Victor G, Tanhehco Elaine J, Silverman Norman, Bernabei Alvise, Sabbah Hani N.
Hypoxia-induced cleavage of caspase-3 and DFF45/ICAD in human failed cardiomyocytes ,
Am J Physiol Heart Circ Physiol
 283 , H990-5 , 2002
Cells
Cardiomyocytes
Enzymes
Protease XIV : 0.013%
Medium
HEPES solution
Heart
Human
Human
Shirai, T. , Rao, V. , Weisel, R. , Ikonomidis, J. , Li, R. , Tumiati, L. , Merante, F. and Mickle, D.
Preconditioning Human Cardiomyocytes and Endothelial Cells. ,
J Thorac Cardiovasc Surg
 115 , 210-9 , 1998
Cells
Cardiomyocytes and endothelial
Enzymes
Trypsin : 0.2%
Collagenase : 0.1%
Medium
PBS
Heart
Human
Human
Hoppe, U. , Jansen, E. , Sudkamp, M. and Beukelmann, D.
Hyperpolarization-Activated Inward Current in Ventricular Myocytes From Normal and Failing Human Hearts ,
Circulation
 97 , 55 , 1998
Cells
Heart
Myocytes
Enzymes
Collagenase Type 2 : 200 u/ml
Medium
Tyrode's solution
Heart
Human
Human
Van Wagoner, D. , Pond, A. , McCarthy, P. , Trimmer, J. and Nerbonne, J.
Outward K+ Current Densities and Kv1.5 Expression are Reduced in Chronic Human Atrial Fibrillation. ,
Circ Res
 80 , 772-81 , 1997
Cells
Atrial myocytes
Enzymes
Protease Type XIV : 0.04%
Medium
see reference
Heart
Human
Human, fetal, 14.5 week gestation
Goldman, B. and Wurzel, J.
Effects of Subcultivation and Culture Medium on Differentiation of Human Fetal Cardiac Myocytes ,
In Vitro Cell Dev Biol
 28 , 109 , 1992
Cells
Myocytes
Enzymes
Hyaluronidase : 0.1%
Medium
HBSS with Calcium
Heart
Human
Human, both sexes, 18-81 years
Smith, D. , Glover, J. , Townsend, L. and Maupin, E.
A Method for the Harvest, Culture, and Characterization of Human Adult Atrial Myocardial Cells: Correlation with Age of Donor ,
In Vitro Cell Dev Biol
 27 , 914 , 1991
Cells
Myocardial
Atrial
Enzymes
Collagenase : 0.14%
Medium
HBSS, CMF
Heart
Human
Human, adult
Bugaisky, L.B.
Biology of Isolated Adult Cardiac Myocytes , Isolation and Culture of Human Adult Cardiac Myocytes , (Clark,Decker,Borg,eds) , Elsevier Science Publishing Co., Inc. , 1988
Cells
Myocytes
Enzymes
Trypsin : 0.25%
Medium
Joklik's MEM
Heart
Human
Human
Hassler, O. , Wiren, M. and Herbertsson, S.
The Elastic Coat of the Arterial Wall Studied with the Aid of Collagenase ,
Acta Pathol Microbiol Scand
 57 , 15 , 1963
Cells
Thoracic aorta
Enzymes
Collagenase : 0.2%
Medium
Phosphate buffer w/NaCl
Stem
Human
Human
Alstrup, T. , Eijken, M. , Bohn, A. , Moller, B. and Damsgaard, T.
Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat. ,
Curr Protoc Stem Cell Biol
 48 , e68 , 2019
Cells
Adipose derived stem
Enzymes
Collagenase Type 4 : 150-300 u/ml
Medium
RPMI-1640
Stem
Human
Human
Yam, G. , Teo, E. , Setiawan, M. , Lovatt, M. , Yusoff, N. , Fuest, M. , Goh, B. and Mehta, J.
Postnatal Periodontal Ligament as a Novel Adult Stem Cell Source for Regenerative Corneal Cell Therapy. ,
J Cell Mol Med
 , 2018
Cells
Postnatal periodontal ligament
Enzymes
Medium
DMEM/F12
Stem
Human
Human
Matsumura, E. , Tsuji, K. , Komori, K. , Koga, H. , Sekiya, I. and Muneta, T.
Pretreatment with IL-1β Enhances Proliferation and Chondrogenic Potential of Synovium-Derived Mesenchymal Stem Cells. ,
Cytotherapy
 19 , 181-193 , 2017
Cells
Synovial mesenchymal stem
Enzymes
Collagenase : 0.3%
Medium
MEM
Stem
Human
Human
Clark, K. , Fierro, F. , Ko, E. , Walker, N. , Arzi, B. , Tepper, C. , Dahlenburg, H. , Cicchetto, A. , Koi, A. , Marsh, L. , Murphy, W. , Fazel, N. and Borjesson, D.
Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome. ,
Stem Cell Res Ther
 8 , 69 , 2017
Cells
Adipose derived mesenchymal stem
Enzymes
Medium
PBS
Stem
Human
Human
de Witte, S. , Lambert, E. , Merino, A. , Strini, T. , Douben, H. , O'Flynn, L. , Elliman, S. , de Klein, A. , Newsome, P. , Baan, C. and Hoogduijn, M.
Aging of Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stromal Cells During Expansion. ,
Cytotherapy
 19 , 798-807 , 2017
Cells
Umbilical cord mesenchymal stromal
Enzymes
Medium
MEM
Stem
Human
Human
Oppliger, B. , Joerger-Messerli, M. , Simillion, C. , Mueller, M. , Surbek, D. and Schoeberlein, A.
Mesenchymal Stromal Cells from Umbilical Cord Wharton's Jelly Trigger Oligodendroglial Differentiation in Neural Progenitor Cells Through Cell-to-Cell Contact. ,
Cytotherapy
 19 , 829-838 , 2017
Cells
Wharton jelly mesenchymal stromal
Enzymes
Collagenase Type 2 : 270 u/ml
Medium
DMEM/F12
Stem
Human
Human
Rigamonti, A. , Repetti, G. , Sun, C. , Price, F. , Reny, D. , Rapino, F. , Weisinger, K. , Benkler, C. , Peterson, Q. , Davidow, L. , Hansson, E. and Rubin, L.
Large-Scale Production of Mature Neurons from Human Pluripotent Stem Cells in a Three-Dimensional Suspension Culture System. ,
Stem Cell Reports
 6 , 993-1008 , 2016
Cells
Motor neurons
Enzymes
Papain : 20 u/ml
Medium
EBSS
Stem
Human
Human
Randelli, P. , Menon, A. , Ragone, V. , Creo P. , Alfieri, M. , Perucca, O. , Banfi, G. , Cabitza, P. , Tettamanti, G. and Anastasia, L.
Effects of the Pulsed Electromagnetic Field PST on Human Tendon Stem Cells: a Controlled Laboratory Study. ,
BMC Complement Altern Med
 16 , 293 , 2016
Cells
Tendon stem
Enzymes
Medium
MEM
Stem
Human
Human
Cheng, N. , Hsieh, T. , Lai, H. and Young, T.
High Glucose-Induced Reactive Oxygen Species Generation Promotes Stemness in Human Adipose-Derived Stem Cells ,
Cytotherapy
 18 , 371-83 , 2016
Cells
Adipose stem
Enzymes
Medium
DMEM/F12
Stem
Human
Human
Lim, J. , Razi, Z. , Law, J. , Nawi, A. , Idrus, R. and Ng, M.
MSCs can be Differentially Isolated From Maternal, Middle and Fetal Segments of the Human Umbilical Cord. ,
Cytotherapy
 18 , 1493-1502 , 2016
Cells
Wharton jelly mesenchymal stromal
Enzymes
Medium
DMEM
Stem
Human
Human
Mothe, A. and Tator, C.
Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord. ,
J Vis Exp
 , e52732 , 2015
Cells
Spinal cord neural progenitor
Enzymes
PDS kit : with modifications
Medium
EBSS
Stem
Human
Human
Wu, Z. , Wang, J. , Dong, R. , Wang, L. , Fan, Z. , Liu, D. and Wang, S.
Depletion of MEIS2 Inhibits Osteogenic Differentiation Potential of Human Dental Stem Cells. ,
Int J Clin Exp Med
 8 , 7220-30 , 2015
Cells
Dental mesenchymal stem
Enzymes
Medium
DMEM
Stem
Human
Human
Kusuma, G. , Menicanin, D. , Gronthos, S. , Manuelpillai, U. , Abumaree, M. , Pertile, M. , Brennecke, S. and Kalionis, B.
Ectopic Bone Formation by Mesenchymal Stem Cells Derived from Human Term Placenta and the Decidua. ,
PLoS ONE
 10 , e0141246 , 2015
Cells
Decidua mesenchymal stem
Enzymes
Medium
PBS
Stem
Human
Human
Satish, L. , Krill-Burger, J. , Gallo, P. , Etages, S. , Liu, F. , Philips, B. , Ravuri, S. , Marra, K. , LaFramboise, W. , Kathju, S. and Rubin, J.
Expression Analysis of Human Adipose-Derived Stem Cells During In Vitro Differentiation to an Adipocyte Lineage. ,
BMC Med Genomics
 8 , 41 , 2015
Cells
Adipose derived stem cells
Enzymes
Medium
HBSS
Stem
Human
Human
Charville, G. , Cheung, T. , Yoo, B. , Santos, P. , Lee, G. , Shrager, J. and Rando, T.
Ex Vivo Expansion and In Vivo Self-Renewal of Human Muscle Stem Cells. ,
Stem Cell Reports
 5 , 621 , 2015
Cells
Skeletal muscle stem
Enzymes
Collagenase Type 2 : 750 u/ml
Neutral Protease : 2 u/ml
Medium
Ham's F10
Stem
Human
Human
Lee, S. , Park, B. , Kim, J. , Jekarl, D. , Choi, H. , Lee, S. , Kim, M. , Kim, Y. and Park, M.
The Effect of Fibroblast Growth Factor on Distinct Differentiation Potential of Cord Blood-Derived Unrestricted Somatic stem Cells and Wharton's Jelly-Derived Mesenchymal Stem/Stromal Cells. ,
Cytotherapy
 17 , 1723-31 , 2015
Cells
Wharton jelly mesenchymal stem
Enzymes
Medium
PBS
Stem
Human
Human
Choudhery,M, Badowski, M, Muise, A. and Harris, D
Effect of Mild Heat Stress on the Proliferative and Differentiative Ability of Human Mesenchymal Stromal Cells. ,
Cytotherapy
 17 , 359-68 , 2015
Cells
Mesenchymal stromal
Enzymes
Medium
PBS
Stem
Human
Human
Najar, M, Rodrigues, R, Buyl, K, Branson, S, Vanhaecke, T, Lagneaux, L, Rogiers, V. and De Kock, J
Proliferative and Phenotypical Characteristics of Human Adipose Tissue-Derived Stem Cells: Comparison of Ficoll Gradient Centrifugation and Red Blood Cell Lysis Buffer Treatment Purification Methods. ,
Cytotherapy
 16 , 1220-8 , 2014
Cells
Mesenchymal stromal
Enzymes
Medium
PBS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.