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Tissue References Search Tool

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Kidney
Human
Human
Basu, J. , Genheimer, C. , Sangha, N. , Quinlan, S. , Guthrie, K. , Kelley, R. , Ilagan, R. , Jain, D. , Bertram, T. and Ludlow, J.
Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. ,
Lipids Health Dis
 10 , 171 , 2011
Cells
Renal adipose derived cells
Enzymes
Medium
DMEM
Kidney
Human
Human, adult
Valente, M. , Henrique, R. , Costa, V. , Jeronimo, C. , Carvalho, F. , Bastos, M. , Guedes de Pinho, P. and Carvalho, M.
A Rapid and Simple Procedure for the Establishment of Human Normal and Cancer Renal Primary Cell Cultures from Surgical Specimens. ,
PLoS ONE
 6 , e19337 , 2011
Cells
Renal tumor cells and proximal tubular epithelial
Enzymes
Medium
DMEM/F12
Kidney
Human
Human
Presnell, S. , Bruce, A. , Wallace, S. , Choudhury, S. , Genheimer, C. , Cox, B. , Guthrie, K. , Werdin, E. , Tatsumi-Ficht, P. , Ilagan, R. , Kelley, R. , Rivera, E. , Ludlow, J. , Wagner, B. , Jayo, M. and Bertram, T.
Isolation, Characterization, and Expansion Methods for Defined Primary Renal Cell Populations from Rodent, Canine, and Human Normal and Diseased Kidneys. ,
Tiss Eng
 17 , 261 , 2011
Cells
Renal cells
Enzymes
Collagenase Type 4 : 300 u/ml
Neutral Protease : see reference
Medium
HBSS
Kidney
Human
Human, adult
Johnson, D. , Saunders, H. , Johnson, F. , Huq, S. , Field, M. and Pollock, C.
Cyclosporin exerts a direct fibrogenic effect on human tubulointerstitial cells: roles of insulin-like growth factor I, transforming growth factor beta1, and platelet-derived growth factor ,
J Pharmacol Exp Ther
 289 , 535-42 , 1999
Cells
Renal proximal tubule and cortical fibroblasts
Enzymes
Collagenase Type 2 : 383 u/ml
Medium
DMEM/F-12
Kidney
Human
Human
McAteer, J, Kempson, S. and Evan, A
Culture of Human Renal Cortex Epithelial Cells ,
J Tiss Cul Meth
 13 , 143 , 1991
Cells
Renal cortex
Enzymes
Trypsin : 0.1%
Medium
Tissue Culture Grade Water
Kidney
Human
Human
Trifillis, A. and Kahng, M.
Characterization of an In Vitro System of Human Renal Papillary Collecting Duct Cells ,
In Vitro Cell Dev Biol
 26 , 441 , 1990
Cells
Papillary duct
Enzymes
Collagenase : 400 u/ml
Medium
Eagle's MEM-HEPES buffer w/L-glutamine
Kidney
Human
Human
Heieren, M. , van der Woude, F. and Balfour Jr., H.
Cytomegalovirus Replicates Efficiently in Human Kidney Mesangial Cells ,
Proc Natl Acad Sci U S A
 85 , 1642 , 1988
Cells
Mesangial
Enzymes
Trypsin : 0.25%
Medium
DMEM/Ham's F-12
Kidney
Human
Human, adult
Yang, A. , Gould-Kostka, J. and Oberley, T.
In Vitro Growth and Differentiation of Human Kidney Tubular Cells on a Basement Membrane Substrate ,
In Vitro Cell Dev Biol
 23 (1) , 34 , 1987
Cells
Tubular
Enzymes
Collagenase : 250 u/ml
Medium
PBS
Kidney
Human
Human, adult, 14-66 years
Trifillis, A. , Regec, A. and Trump, B.
Isolation, Culture, and Charaterization of Human Renal Tubular Cells ,
J Urol
 133 , 324 , 1985
Cells
Tubular
Enzymes
Collagenase : 100 u/ml
Medium
Joklik's MEM
Kidney
Human
Human
Hemstreet, G. , Enoch, P. and Pretlow, T.
Tissue Disaggregation of Human Renal Cell Carcinoma with Further Isopyknic and Isokinetic Gradient Purification ,
Cancer Res
 40 , 1043 , 1980
Cells
Malignant
Stromal
Enzymes
Papain : 0.009%
Medium
Sacks solution
Kidney
Human
Human, 24 newborn and stillborn
(also rabbit)
De Oca, H. and Malinin, T.
Dispersion and Cultivation of Renal Cells After Short-Term Storage of Kidneys ,
J Clin Microbiol
 2 , 243 , 1975
Cells
Renal
Enzymes
Trypsin : 0.25%
Medium
See reference
Stem
Human
Human
Alstrup, T. , Eijken, M. , Bohn, A. , Moller, B. and Damsgaard, T.
Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat. ,
Curr Protoc Stem Cell Biol
 48 , e68 , 2019
Cells
Adipose derived stem
Enzymes
Collagenase Type 4 : 150-300 u/ml
Medium
RPMI-1640
Stem
Human
Human
Yam, G. , Teo, E. , Setiawan, M. , Lovatt, M. , Yusoff, N. , Fuest, M. , Goh, B. and Mehta, J.
Postnatal Periodontal Ligament as a Novel Adult Stem Cell Source for Regenerative Corneal Cell Therapy. ,
J Cell Mol Med
 , 2018
Cells
Postnatal periodontal ligament
Enzymes
Medium
DMEM/F12
Stem
Human
Human
Matsumura, E. , Tsuji, K. , Komori, K. , Koga, H. , Sekiya, I. and Muneta, T.
Pretreatment with IL-1β Enhances Proliferation and Chondrogenic Potential of Synovium-Derived Mesenchymal Stem Cells. ,
Cytotherapy
 19 , 181-193 , 2017
Cells
Synovial mesenchymal stem
Enzymes
Collagenase : 0.3%
Medium
MEM
Stem
Human
Human
Clark, K. , Fierro, F. , Ko, E. , Walker, N. , Arzi, B. , Tepper, C. , Dahlenburg, H. , Cicchetto, A. , Koi, A. , Marsh, L. , Murphy, W. , Fazel, N. and Borjesson, D.
Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome. ,
Stem Cell Res Ther
 8 , 69 , 2017
Cells
Adipose derived mesenchymal stem
Enzymes
Medium
PBS
Stem
Human
Human
de Witte, S. , Lambert, E. , Merino, A. , Strini, T. , Douben, H. , O'Flynn, L. , Elliman, S. , de Klein, A. , Newsome, P. , Baan, C. and Hoogduijn, M.
Aging of Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stromal Cells During Expansion. ,
Cytotherapy
 19 , 798-807 , 2017
Cells
Umbilical cord mesenchymal stromal
Enzymes
Medium
MEM
Stem
Human
Human
Oppliger, B. , Joerger-Messerli, M. , Simillion, C. , Mueller, M. , Surbek, D. and Schoeberlein, A.
Mesenchymal Stromal Cells from Umbilical Cord Wharton's Jelly Trigger Oligodendroglial Differentiation in Neural Progenitor Cells Through Cell-to-Cell Contact. ,
Cytotherapy
 19 , 829-838 , 2017
Cells
Wharton jelly mesenchymal stromal
Enzymes
Collagenase Type 2 : 270 u/ml
Medium
DMEM/F12
Stem
Human
Human
Rigamonti, A. , Repetti, G. , Sun, C. , Price, F. , Reny, D. , Rapino, F. , Weisinger, K. , Benkler, C. , Peterson, Q. , Davidow, L. , Hansson, E. and Rubin, L.
Large-Scale Production of Mature Neurons from Human Pluripotent Stem Cells in a Three-Dimensional Suspension Culture System. ,
Stem Cell Reports
 6 , 993-1008 , 2016
Cells
Motor neurons
Enzymes
Papain : 20 u/ml
Medium
EBSS
Stem
Human
Human
Randelli, P. , Menon, A. , Ragone, V. , Creo P. , Alfieri, M. , Perucca, O. , Banfi, G. , Cabitza, P. , Tettamanti, G. and Anastasia, L.
Effects of the Pulsed Electromagnetic Field PST on Human Tendon Stem Cells: a Controlled Laboratory Study. ,
BMC Complement Altern Med
 16 , 293 , 2016
Cells
Tendon stem
Enzymes
Medium
MEM
Stem
Human
Human
Cheng, N. , Hsieh, T. , Lai, H. and Young, T.
High Glucose-Induced Reactive Oxygen Species Generation Promotes Stemness in Human Adipose-Derived Stem Cells ,
Cytotherapy
 18 , 371-83 , 2016
Cells
Adipose stem
Enzymes
Medium
DMEM/F12
Stem
Human
Human
Lim, J. , Razi, Z. , Law, J. , Nawi, A. , Idrus, R. and Ng, M.
MSCs can be Differentially Isolated From Maternal, Middle and Fetal Segments of the Human Umbilical Cord. ,
Cytotherapy
 18 , 1493-1502 , 2016
Cells
Wharton jelly mesenchymal stromal
Enzymes
Medium
DMEM
Stem
Human
Human
Mothe, A. and Tator, C.
Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord. ,
J Vis Exp
 , e52732 , 2015
Cells
Spinal cord neural progenitor
Enzymes
PDS kit : with modifications
Medium
EBSS
Stem
Human
Human
Wu, Z. , Wang, J. , Dong, R. , Wang, L. , Fan, Z. , Liu, D. and Wang, S.
Depletion of MEIS2 Inhibits Osteogenic Differentiation Potential of Human Dental Stem Cells. ,
Int J Clin Exp Med
 8 , 7220-30 , 2015
Cells
Dental mesenchymal stem
Enzymes
Medium
DMEM
Stem
Human
Human
Kusuma, G. , Menicanin, D. , Gronthos, S. , Manuelpillai, U. , Abumaree, M. , Pertile, M. , Brennecke, S. and Kalionis, B.
Ectopic Bone Formation by Mesenchymal Stem Cells Derived from Human Term Placenta and the Decidua. ,
PLoS ONE
 10 , e0141246 , 2015
Cells
Decidua mesenchymal stem
Enzymes
Medium
PBS
Stem
Human
Human
Satish, L. , Krill-Burger, J. , Gallo, P. , Etages, S. , Liu, F. , Philips, B. , Ravuri, S. , Marra, K. , LaFramboise, W. , Kathju, S. and Rubin, J.
Expression Analysis of Human Adipose-Derived Stem Cells During In Vitro Differentiation to an Adipocyte Lineage. ,
BMC Med Genomics
 8 , 41 , 2015
Cells
Adipose derived stem cells
Enzymes
Medium
HBSS
Stem
Human
Human
Charville, G. , Cheung, T. , Yoo, B. , Santos, P. , Lee, G. , Shrager, J. and Rando, T.
Ex Vivo Expansion and In Vivo Self-Renewal of Human Muscle Stem Cells. ,
Stem Cell Reports
 5 , 621 , 2015
Cells
Skeletal muscle stem
Enzymes
Collagenase Type 2 : 750 u/ml
Neutral Protease : 2 u/ml
Medium
Ham's F10
Stem
Human
Human
Lee, S. , Park, B. , Kim, J. , Jekarl, D. , Choi, H. , Lee, S. , Kim, M. , Kim, Y. and Park, M.
The Effect of Fibroblast Growth Factor on Distinct Differentiation Potential of Cord Blood-Derived Unrestricted Somatic stem Cells and Wharton's Jelly-Derived Mesenchymal Stem/Stromal Cells. ,
Cytotherapy
 17 , 1723-31 , 2015
Cells
Wharton jelly mesenchymal stem
Enzymes
Medium
PBS
Stem
Human
Human
Choudhery,M, Badowski, M, Muise, A. and Harris, D
Effect of Mild Heat Stress on the Proliferative and Differentiative Ability of Human Mesenchymal Stromal Cells. ,
Cytotherapy
 17 , 359-68 , 2015
Cells
Mesenchymal stromal
Enzymes
Medium
PBS
Stem
Human
Human
Najar, M, Rodrigues, R, Buyl, K, Branson, S, Vanhaecke, T, Lagneaux, L, Rogiers, V. and De Kock, J
Proliferative and Phenotypical Characteristics of Human Adipose Tissue-Derived Stem Cells: Comparison of Ficoll Gradient Centrifugation and Red Blood Cell Lysis Buffer Treatment Purification Methods. ,
Cytotherapy
 16 , 1220-8 , 2014
Cells
Mesenchymal stromal
Enzymes
Medium
PBS
Stem
Human
Human
Douvaras, P. , Wang, J. , Zimmer, M. , Hanchuk, S. , O'Bara, M. , Sadiq, S. , Sim, F. , Goldman, J. and Fossati, V.
Efficient Generation of Myelinating Oligodendrocytes from Primary Progressive Multiple Sclerosis Patients by Induced Pluripotent Stem Cells. ,
Stem Cell Reports
 3 , 250 , 2014
Cells
Skin fibroblasts
Enzymes
Collagenase Type 1 : 1000 u/ml
Medium
DMEM
Stem
Human
Human
Hang, H. , Yu, Y. , Wu, N. , Huang, Q. , Xia, Q. and Bian, J.
Induction of Highly Functional Hepatocytes from Human Umbilical Cord Mesenchymal Stem Cells by HNF4α Transduction. ,
PLoS ONE
 9 , e104133 , 2014
Cells
Umbilical mesenchymal stem
Enzymes
Trypsin : 0.25%
Medium
DMEM
Stem
Human
Human, adult
Koellensperger, E. , Bollinger, N. , Dexheimer, V. , Gramley, F. , Germann, G. and Leimer, U.
Choosing the Right Type of Serum for Different Applications of Human Adipose Tissue-Derived Stem Cells: Influence on Proliferation and Differentiation Abilities. ,
Cytotherapy
 16 , 789 , 2014
Cells
Adipose derived stem
Enzymes
Medium
DMEM
Stem
Human
Human, adult
Al-Saqi, S. , Saliem, M. , Asikainen, S. , Quezada, H. , Ekblad, A. , Hovatta, O. , Le Blanc, K. , Jonasson, A. and Gotherstrom, C.
Defined Serum-Free Media for In Vitro Expansion of Adipose-Derived Mesenchymal Stem Cells. ,
Cytotherapy
 16 , 915 , 2014
Cells
Adipose derived mesenchymal stem
Enzymes
Medium
see reference
Stem
Human
Human
Avolio, E. , Gianfranceschi, G. , Cesselli, D. , Caragnano, A. , Athanasakis, E. , Katare, R. , Meloni, M. , Palma, A. , Barchiesi, A. , Vascotto, C. , Toffoletto, B. , Mazzega, E. , Finato, N. , Aresu, G. and Livi, U.
Ex vivo Molecular Rejuvenation Improves the Therapeutic Activity of Senescent Human Cardiac Stem Cells in a Mouse Model of Myocardial Infarction. ,
Stem Cells
 32 , 2373 , 2014
Cells
Cardiac stem
Enzymes
Medium
Joklik modified Eagle’s
Stem
Human
Human
Barkauskas, C. , Cronce, M. , Rackley, C. , Bowie, E. , Keene, D. , Stripp, B. , Randell, S. , Noble, P. and Hogan, B.
Type 2 Alveolar Cells are Stem Cells in Adult Lung ,
J Clin Invest
 123 , 3025 , 2013
Cells
Alveolar epithelial
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 0.25%
Elastase : 10 u/ml
Medium
Bronchial Epithelial Growth Medium
Stem
Human
Human
Dobbin, Z. and Landen, C.
Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors--Potential Applications. ,
Curr Protoc Pharmacol
 63 , Unit 14.28. , 2013
Cells
Cancer stem
Enzymes
Trypsin : 0.25%
Collagenase : 0.05%
Hyaluronidase : 0.005%
Medium
RPMI

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.