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Tissue References Search Tool

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Lung
Human

Human

Konishi, S. , Tata, A. and Tata, P.
Defined Conditions for Long-term Expansion of Murine and Human Alveolar Epithelial Stem Cells in Three-dimensional Cultures. , STAR Protoc Vol. 3 , 101447 , 2022
Cells
Alveolar epithelial stem
Enzymes
Collagenase Type 1 : 450 u/ml
Neutral Protease : 5 u/ml
Deoxyribonuclease I : 0.33 u/ml
Medium
DMEM/F12
Lung
Human

Human

Youk, J. , Kim, T. , Evans, K. , Jeong, Y. , Hur, Y. , Hong, S. , Kim, J. , Yi, K. , Kim, S. , Na, K. , Bleazard, T. , Kim, H. , Fellows, M. , Mahbubani, K. , Saeb-Parsy, K. , Kim, S. , Kim, Y. , Koh, G. , Choi, B. , Ju, Y. and Lee, J.
Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2. , Cell Stem Cell Vol. 27 , 905-919.e10 , 2020
Cells
Aveolar
Enzymes
Medium
DMEM
Lung
Human
Human
Bahmed, K. , Boukhenouna, S. , Karim, L. , Andrews, T. , Lin, J. , Powers, R. , Wilson, M. , Lin, C. , Messier, E. , Reisdorph, N. , Powell, R. , Tang, H. , Mason, R. , Criner, G. and Kosmider, B.
The Effect of Cysteine Oxidation on DJ-1 Cytoprotective Function in Human Alveolar Type II Cells. ,
Cell Death Dis
 10 , 638 , 2019
Cells
Aveolar
Enzymes
Elastase : 12.9 u/ml
Medium
PBS
Lung
Human
Human
Quatromoni, J. , Singhal, S. , Bhojnagarwala, P. , Hancock, W. , Albelda, S. and Eruslanov, E.
An Optimized Dissagregation Method for Human Lung Tumors That Perserves the Phenotype and Function of the Immune Cells ,
J Leukoc Biol
 97 , 201 , 2015
Cells
Lung tumor
Enzymes
Collagenase Type 1 : 45-60 u/ml
Collagenase Type 2 : 15-20 u/ml
Collagenase Type 4 : 45-60 u/ml
Elastase : 0.002%
Medium
DMEM/F12
Lung
Human
Human
Barkauskas, C. , Cronce, M. , Rackley, C. , Bowie, E. , Keene, D. , Stripp, B. , Randell, S. , Noble, P. and Hogan, B.
Type 2 Alveolar Cells are Stem Cells in Adult Lung ,
J Clin Invest
 123 , 3025 , 2013
Cells
Alveolar epithelial
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 0.25%
Elastase : 10 u/ml
Medium
Bronchial Epithelial Growth Medium
Lung
Human
Human
Mackay, L. , Dodd, S. , Dougall, I. , Tomlinson, W. , Lordan, J. , Fisher, A. and Corris, P.
Isolation and Characterisation of Human Pulmonary Microvascular Endothelial Cells from Patients with Severe Emphysema. ,
Respir Res
 14 , 23 , 2013
Cells
Pulmonary endothelial
Enzymes
Medium
RPMI
Lung
Human
Human
Comhair, S. , Xu, W. , Mavrakis, L. , Aldred, M. , Asosingh, K. and Erzurum, S.
Human Primary Lung Endothelial Cells in Culture. ,
Am J Respir Cell Mol Biol
 46 , 723-30 , 2012
Cells
Lung endothelial
Enzymes
Medium
PBS
Lung
Human
Human
Fujino, N. , Kubo, H. , Suzuki, T. , Ota, C. , Hegab, A. , He, M. , Suzuki, S. , Suzuki, T. , Yamada, M. , Kondo, T. , Kato, H. and Yamaya, M.
Isolation of Alveolar Epithelial Type II Progenitor Cells from Adult Human Lungs. ,
Lab Invest
 91 , 363 , 2011
Cells
Lung
Enzymes
Medium
DMEM
Lung
Human
Human
Zhu, Y. , Skold, C. , Liu, X. , Wang, H. , Kohyama. , Wen, F. , Ertl, R. and Rennard, S.
Fibroblasts and Monocyte Macrophages Contract and Degrade Three-Dimensional Collagen Gels in Extended Co-Culture ,
Respir Res
 2 (5) , 295 , 2001
Cells
Lung
Fibroblasts
Enzymes
Trypsin : 0.05%
Medium
DMEM
Lung
Human
Human, 16-24 week therapeutic abortions
Liley, H. , Ertsey, R. , Gonzales, L. , Odom, M. , Hawgood, S. , Dobbs, L. and Ballard, P.
Synthesis of Surfactant Components by Cultured Type II Cells From Human Lung ,
Biochim Biophys Acta
 961 , 86 , 1988
Cells
Alveolar type II
Fetal
Enzymes
Trypsin : 50 ug/ml
Medium
Ham's F-12, Eagle's MEM
Lung
Human
Human
Lechner, J. , Haugen, A. , McClendon, I. and Pettis, E.
Clonal Growth of Normal Adult Human Bronchial Epithelial Cells in a Serum-Free Medium ,
In Vitro
 18 (7) , 633 , 1982
Cells
Epithelial
Enzymes
Trypsin : 0.02%
Medium
Medium 199
Lung
Human
Human, 12-16 wk old embryo
Kan, M. and Yamane, I.
In Vitro Proliferation and Lifespan of Human Diploid Fibroblasts in Serum-Free BSA-Containing Medium ,
J Cell Physiol
 111 , 155 , 1982
Cells
Fibroblasts
Enzymes
Trypsin : 0.01%
Medium
Eagle's MEM
Lung
Human
Human fetuses, 80 day
(also swine fetuses, 70 day, adult Amer Dutch, 250 day)
Hinz, R. and Syverton, J.
Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase ,
Proc Soc Exp Biol Med
 101 , 19 , 1959
Cells
Lung
Enzymes
Collagenase : 0.01%
Medium
HBSS
Muscle
Human
Human
Malysz, J. , Rovner, E. , Wake, R. and Petkov, G.
Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents. ,
J Vis Exp
 , 2020
Cells
Bladder smooth muscle
Enzymes
Collagenase Type 2 : 0.1-0.2%
Papain : 0.1-0.2%
Medium
Digestion solution (see reference)
Muscle
Human
Human
Garcia, S. , Tamaki, S. , Lee, S. , Wong, A. , Jose, A. , Dreux, J. , Kouklis, G. , Sbitany, H. , Seth, R. , Knott, P. , Heaton, C. , Ryan, W. , Kim, E. , Hansen, S. , Hoffman, W. and Pomerantz, J.
High-Yield Purification, Preservation, and Serial Transplantation of Human Satellite Cells. ,
Stem Cell Reports
 10 , 1160-1174 , 2018
Cells
Muscle satellite
Enzymes
Collagenase : 0.1%
Trypsin : 0.25%
Medium
DMEM
Muscle
Human
Human
Charville, G. , Cheung, T. , Yoo, B. , Santos, P. , Lee, G. , Shrager, J. and Rando, T.
Ex Vivo Expansion and In Vivo Self-Renewal of Human Muscle Stem Cells. ,
Stem Cell Reports
 5 , 621 , 2015
Cells
Skeletal muscle stem
Enzymes
Collagenase Type 2 : 750 u/ml
Neutral Protease : 2 u/ml
Medium
Ham's F10
Muscle
Human
Human
Lu, S. , Sun, X. , Hong, T. , Song, K. , Yang, S. and Wang, C.
Isolation and Culture of Smooth Muscle Cells from Human Acute Type A Aortic Dissection. ,
J Cardiothorac Surg
 8 , 83 , 2013
Cells
Smooth muscle
Enzymes
Medium
DMEM
Muscle
Human
Human, male
Stadler, G. , Chen, J. , Wagner, K. , Robin, J. , Shay, J. , Emerson, C. and Wright, W.
Establishment of Clonal Myogenic Cell Lines from Severely Affected Dystrophic Muscles - CDK4 Maintains the Myogenic Population. ,
Skelet Muscle
 1 , 12 , 2011
Cells
Myogenic
Enzymes
Medium
HBSS
Muscle
Human
Human
Nesti, L. , Jackson, W. , Shanti, R. , Koehler, S. , Aragon, A. , Bailey, J. , Sracic, M. , Freedman, B. , Giuliani, J. and Tuan, R.
Differentiation Potential of Multipotent Progenitor Cells Derived from War-Traumatized Muscle Tissue. ,
J Bone Joint Surg Am
 90 , 2390 , 2008
Cells
Muscle derived multiprogenitor cells
Enzymes
Medium
DMEM
Muscle
Human
Human
Moss, S. , Bates, M. , Parrino, P. and Woods, TC.
Isolation of Endothelial Cells and Vascular Smooth Muscle Cells from Internal Mammary Artery Tissue. ,
Ochsner J
 7 , 133 , 2007
Cells
Endothelial and vascular smooth muscle
Enzymes
Medium
HBSS
Muscle
Human
Human
Kimuli, M. , Eardley, I. and Southgate, J.
In Vitro Assessment of Decellularized Porcine Dermis as a Matrix for Urinary Tract Reconstruction. ,
BJU Int
 94 , 859 , 2004
Cells
Urinary tract smooth muscle
Enzymes
Collagenase Type 4 : 100 u/ml
Medium
DMEM
Muscle
Human
Human, female
Richardson, M. , Taylor, D. , Casey, M. , MacDonald, P. and Stull, J.
Biochemical Markers of Contraction in Human Myometrial Smooth Muscle Cells in Culture ,
In Vitro Cell Dev Biol
 23 , 21 , 1987
Cells
Smooth muscle
Myometrial
Enzymes
Deoxyribonuclease I : 0.015% and 0.007%
Medium
HBSS
Muscle
Human
Human, female
Casey, M. , MacDonald, P. , Mitchell, M. and Snyder, J.
Maintenance and Characterization of Human Myometrial Smooth Muscle Cells in Monolayer Culture ,
In Vitro
 20 , 396 , 1984
Cells
Smooth muscle
Myometrial
Enzymes
Medium
HBSS
Muscle
Human
Human, fetal
(also bovine)
Davies, P. and Kerr, C.
Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts ,
Biochim Biophys Acta
 712 , 26 , 1982
Cells
Smooth muscle, fibroblasts
Enzymes
Trypsin : 0.055%
Medium
DMEM
Muscle
Human
Human
Eskin, S. , Sybers, H. , Lester, J. , Navarro, L. , Gotto, A. and DeBakey, M.
Human Smooth Muscle Cells Cultured From Atherosclerotic Plaques and Uninvolved Vessel Wall ,
In Vitro
 17 (8) , 713 , 1981
Cells
Smooth muscle
Enzymes
Trypsin : 0.25%
Medium
DMEM
Muscle
Human
Human
(also rat, guinea-pig, chick, monkey)
Chamley-Campbell, J. , Campbell, G. and Ross, R.
The Smooth Muscle in Cell Culture ,
Physiol Res
 59 , 1 , 1979
Cells
Smooth muscle
Enzymes
Trypsin : 0.05% - 0.1%
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.