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Tissue References Search Tool

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Organoids
Human

Human

Gamero-Estevez, E. , Hensel, I. , Steinhauer, M. , Müllertz, O. , Savochkina, E. , Sektioglu, I. , Stoll, B. , Derakhshani, S. , Devriese, S. , Kim, K. and Resnik-Docampo, M.
Protocol for Immune Cell Isolation, Organoid Generation, and Co-culture Establishment from Cryopreserved Whole Human Intestine. ,
Bio Protoc
 15 , e5157 , 2025
Cells
Intestinal organoids
Enzymes
Medium
RPMI
Organoids
Human

Human

Geest, A. , Jakobs, C. , Ljubikj, T. , Huffels, C. , Luna, M. , Sá, R. , Adolfs, Y. , Wit, M. , Rutten, D. , Kaal, M. , Zwartkruis, M. , Carcolé, M. , Groen, E. , Hol, E. , Basak, O. , Isaacs, A. , Westeneng, H. , Berg, L. , Veldink, J. , Schlegel, D. and Pasterkamp, R.
Molecular Pathology, Developmental Changes, and Synaptic Dysfunction in (pre-) Symptomatic Human C9ORF72-ALS/FTD Cerebral Organoids. , Acta Neuropathol Commun Vol. 12 , 152 , 2024
Cells
Cerebral organoids
Enzymes
Papain : 20 u/ml
Medium
DMEM/F12
Organoids
Human

Human

Lisowski, P. , Lickfett, S. , Rybak-Wolf, A. , Menacho, C. and Le, S.
Mutant Huntingtin Impairs Neurodevelopment in Human Brain Organoids through CHCHD2-mediated Neurometabolic Failure. ,
Nat Commun
 15 , 7027 , 2024
Cells
Brain organoids
Enzymes
Papain : 20 u/ml
Medium
EBSS
Organoids
Human

Human

Tresenrider, A. , Sridhar, A. , Eldred, K. , Cuschieri, S. , Hoffer, D. , Trapnell, C. and Reh, T.
Single-cell Sequencing of Individual Retinal Organoids Reveals Determinants of Cell Fate Heterogeneity. , bioRxiv , 2023
Cells
Retinal Organoids
Enzymes
PDS kit : see reference
Medium
DMEM
Organoids
Human

Human

Chiu, M. , Li, C. , Yu, Y. , Liu, X. , Huang, J. , Wan, Z. , Yuen, K. and Zhou, J.
Establishing Bipotential Human Lung Organoid Culture System and Differentiation to Generate Mature Alveolar and Airway Organoids. ,
Bio Protoc
 13 , e4657 , 2023
Cells
Lung Organoids
Enzymes
Medium
Basal medium
Organoids
Human

Human, adolescent

Hemeryck, L. , Hermans, F. , Chappell, J. , Kobayashi, H. , Lambrechts, D. , Lambrichts, I. , Bronckaers, A. and Vankelecom, H.
Organoids from Human Tooth Showing Epithelial Stemness Phenotype and Differentiation Potential. ,
Cell Mol Life Sci
 79 , 153 , 2022
Cells
Epithelial organoids
Enzymes
Medium
MEM
Organoids
Human

Human

Schröter, M. , Wang, C. , Terrigno, M. , Hornauer, P. , Huang, Z. , Jagasia, R. and Hierlemann, A.
Functional Imaging of Brain Organoids Using High-Density Microelectrode Arrays. , MRS Bull Vol. 47 , 530-544 , 2022
Cells
Cerebral organoids
Enzymes
PDS kit : see reference
Medium
PBS
Organoids
Human

Human

Uzquiano, A. , Kedaigle, A. , Pigoni, M. , Paulsen, B. , Adiconis, X. , Kim, K. , Faits, T. , Nagaraja, S. , Antón-Bolaños, N. , Gerhardinger, C. , Tucewicz, A. , Murray, E. , Jin, X. , Buenrostro, J. , Chen, F. , Velasco, S. , Regev, A. , Levin, J. and Arlotta, P.
Proper Acquisition of Cell Class Identity in Organoids Allows Definition of Fate Specification Programs of the Human Cerebral Cortex ,
Cell
 185 , 3770-3788.e27 , 2022
Cells
Brain organoids
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Xue, Y. , Seiler, M. , Tang, W. , Wang, J. , Delgado, J. , McLelland, B. , Nistor, G. , Keirstead, H. and Browne, A.
Retinal Organoids On-a-Chip: a Micro-Millifluidic Bioreactor for Long-term Organoid Maintenance. ,
Lab Chip
 21 , 3361-3377 , 2021
Cells
Stem cell-derived retinal organoids
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Vaart, J. , Böttinger, L. , Geurts, M. , Wetering, W. , Knoops, K. , Sachs, N. , Begthel, H. , Korving, J. , Lopez-Iglesias, C. , Peters, P. , Eitan, K. , Gileles-Hillel, A. and Clevers, H.
Modelling of Primary Ciliary Dyskinesia using Patient-derived Airway Organoids. , EMBO Rep Vol. 22 , e52058 , 2021
Cells
Airway organoids
Enzymes
Pronase : 0.15%
Collagenase : 0.05%
Medium
Airway medium
Organoids
Human

Human

Marinucci, M. , Ercan, C. , Taha-Mehlitz, S. , Fourie, L. , Panebianco, F. , Bianco, G. , Gallon, J. , Staubli, S. , Soysal, S. , Zettl, A. , Rauthe, S. , Vosbeck, J. , Droeser, R. , Bolli, M. , Peterli, R. , Flüe, M. , Ng, C. , Kollmar, O. , Coto-Llerena, M. and Piscuoglio, S.
Standardizing Patient-Derived Organoid Generation Workflow to Avoid Microbial Contamination From Colorectal Cancer Tissues. , Front Oncol Vol. 11 , 781833 , 2021
Cells
Colorectal cancer organoids
Enzymes
Medium
DMEM/F-12
Organoids
Human

Human

Schreurs, R. , Baumdick, M. , Drewniak, A. and Bunders, M.
Co-culture of Human Intestinal Organoids and Lamina Propria-derived CD4 T Cells. , STAR Protoc Vol. 2 , 100519 , 2021
Cells
Lamina propria lymphocytes
Enzymes
Medium
DMEM/F-12
Organoids
Human

Human

Barbar, L. , Rusielewicz, T. , Zimmer, M. , Kalpana, K. and Fossati, V.
Isolation of Human CD49f Astrocytes and iPSC-Based Neurotoxicity Assays. , STAR Protoc Vol. 1 , 100172 , 2020
Cells
Astrocytes
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Velasco, S. , Kedaigle, A. , Simmons, S. , Nash, A. , Rocha, M. , Quadrato, G. , Paulsen, B. , Nguyen, L. , Adiconis, X. , Regev, A. , Levin, J. and Arlotta, P.
Individual Brain Organoids Reproducibly Form Cell Diversity of the Human Cerebral Cortex. ,
Nature
 570 , 523-527 , 2019
Cells
Brain organoids
Enzymes
PDS kit : see reference
Medium
PBS
Organoids
Human

Human

Mansour, A. , Gonçalves, J. , Bloyd, C. , Li, H. , Fernandes, S. , Quang, D. , Johnston, S. , Parylak, S. , Jin, X. and Gage, F.
An In Vivo Model of Functional and Vascularized Human Brain Organoids. ,
Nat Biotechnol
 36 , 432-441 , 2018
Cells
Cerebral organoids
Enzymes
Medium
DMEM/F12
Organoids
Human

Human, embryonic

Nikolić, M. , Caritg, O. , Jeng, Q. , Johnson, J. , Sun, D. , Howell, K. , Brady, J. , Laresgoiti, U. , Allen, G. , Butler, R. , Zilbauer, M. , Giangreco, A. and Rawlins, E.
Human Embryonic Lung Epithelial Tips are Multipotent Progenitors that can be Expanded In vitro as Long-term Self-renewing Organoids. ,
Elife
 6 , 2017
Cells
Lung organoids
Enzymes
Medium
DMEM/F12
Pancreas
Human
Human
Brissova, M. , Haliyur, R. , Saunders, D. , Shrestha, S. , Dai, C. , Blodgett, D. , Bottino, R. , Campbell-Thompson, M. , Aramandla, R. , Poffenberger, G. , Lindner, J. , Pan, F. , von Herrath, M. , Greiner, D. and Shultz, L.
α Cell Function and Gene Expression Are Compromised in Type 1 Diabetes. ,
Cell Rep
 22 , 2667-2676 , 2018
Cells
Islets
Enzymes
Collagenase Type 1 : 1,600u/isolation
Neutral Protease : 200 u/isolation
Deoxyribonuclease I : 12,000 u/isolation
Medium
RPMI
Pancreas
Human
Human
Cane, M. , Sutton, R. and Criddle, D.
Isolation of Human Pancreatic Acinar Cells From Rat and Human Pancreas ,
Pancreapedia
 20 , 1 , 2011
Cells
Acinar
Enzymes
CLSPA : 200 u/ml
Medium
See Reference
Pancreas
Human
Human
Li, C. , Heidt, D. , Dalerba, P. , Burant, C. , Zhang, L. , Adsay, V. , Wicha, M. , Clarke, M. and Simeone, D.
Identification of Pancreatic Cancer Stem Cells. ,
Cancer Res
 67 , 1030 , 2007
Cells
Pancreatic cancer stem cells
Enzymes
Collagenase Type 4 : 200 u/ml
Medium
medium 199
Pancreas
Human
Human, 25-55 yrs old
(also porcine)
Contractor, H. , Johnson, P. , Chadwick, D. , Robertson, G. and London, N.
The Effect of UW Solution and its Components on the Collagenase Digestion of Human and Porcine Pancreas ,
Cell Transplant
 4 , 615 , 1995
Cells
Islets
Enzymes
Collagenase : 0.4%
Medium
HBSS
Pancreas
Human
Human
Watt, P. , Mullen, Y. , Benhamou, P. , Hober, C. , Nomura, Y. , Watanabe, Y. , Passaro, E. , Zinner, M. and Brunicardi, F.
Simplified Semiautomated Method For Isolating Islets From the Human Pancreas ,
Transplant Proc
 26 (2) , 582 , 1994
Cells
Islets
Enzymes
Trypsin : Trypsin: 0.125-0.25%
Collagenase : 0.2%
Deoxyribonuclease I : 200 u/ml
Medium
Eurocollins solution
Pancreas
Human
Human, adult organ donors
Warnock, G. , Rajotte, R. , Evans, M. , Ellis, D. , DeGroot, T. and Dawidson, I.
Isolation of Islets of Langerhans Following Cold Storage of Human Pancreas ,
Transplant Proc
 XIX (4) , 3466 , 1987
Cells
Islets
Enzymes
Collagenase : 0.6%
Medium
Eurocollins solution
Pancreas
Human
Human
Izumi, R. , Konishi, K. , Ueno, K. , Shimizu, K. , Hirosawa, H. , Takahashi, N. and Miyazaki, I.
Isolation of Human Pancreatic Islets from Cryopreserved Pancreas ,
Transplant Proc
 XVII , 383 , 1985
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Human
Human
Gray, D. , McShane, P. , Grant, A. and Morris, P.
A Method for Isolation of Islets of Langerhans from the Human Pancreas ,
Diabetes
 33 , 1055 , 1984
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Human
Human, infant, age 1 day-1 year
Sutherland, D. , Matas, A. , Steffes, M. and Najarian, J.
Infant Human Pancreas: A Potential Source of Islet Tissue for Transplantation ,
Diabetes
 25 (12) , 1123 , 1976
Cells
Islets
Enzymes
Collagenase : 170-210 u/ml
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.