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Tissue References Search Tool

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Organoids
Human

Human

Gamero-Estevez, E. , Hensel, I. , Steinhauer, M. , Müllertz, O. , Savochkina, E. , Sektioglu, I. , Stoll, B. , Derakhshani, S. , Devriese, S. , Kim, K. and Resnik-Docampo, M.
Protocol for Immune Cell Isolation, Organoid Generation, and Co-culture Establishment from Cryopreserved Whole Human Intestine. ,
Bio Protoc
 15 , e5157 , 2025
Cells
Intestinal organoids
Enzymes
Medium
RPMI
Organoids
Human

Human

Geest, A. , Jakobs, C. , Ljubikj, T. , Huffels, C. , Luna, M. , Sá, R. , Adolfs, Y. , Wit, M. , Rutten, D. , Kaal, M. , Zwartkruis, M. , Carcolé, M. , Groen, E. , Hol, E. , Basak, O. , Isaacs, A. , Westeneng, H. , Berg, L. , Veldink, J. , Schlegel, D. and Pasterkamp, R.
Molecular Pathology, Developmental Changes, and Synaptic Dysfunction in (pre-) Symptomatic Human C9ORF72-ALS/FTD Cerebral Organoids. , Acta Neuropathol Commun Vol. 12 , 152 , 2024
Cells
Cerebral organoids
Enzymes
Papain : 20 u/ml
Medium
DMEM/F12
Organoids
Human

Human

Lisowski, P. , Lickfett, S. , Rybak-Wolf, A. , Menacho, C. and Le, S.
Mutant Huntingtin Impairs Neurodevelopment in Human Brain Organoids through CHCHD2-mediated Neurometabolic Failure. ,
Nat Commun
 15 , 7027 , 2024
Cells
Brain organoids
Enzymes
Papain : 20 u/ml
Medium
EBSS
Organoids
Human

Human

Tresenrider, A. , Sridhar, A. , Eldred, K. , Cuschieri, S. , Hoffer, D. , Trapnell, C. and Reh, T.
Single-cell Sequencing of Individual Retinal Organoids Reveals Determinants of Cell Fate Heterogeneity. , bioRxiv , 2023
Cells
Retinal Organoids
Enzymes
PDS kit : see reference
Medium
DMEM
Organoids
Human

Human

Chiu, M. , Li, C. , Yu, Y. , Liu, X. , Huang, J. , Wan, Z. , Yuen, K. and Zhou, J.
Establishing Bipotential Human Lung Organoid Culture System and Differentiation to Generate Mature Alveolar and Airway Organoids. ,
Bio Protoc
 13 , e4657 , 2023
Cells
Lung Organoids
Enzymes
Medium
Basal medium
Organoids
Human

Human, adolescent

Hemeryck, L. , Hermans, F. , Chappell, J. , Kobayashi, H. , Lambrechts, D. , Lambrichts, I. , Bronckaers, A. and Vankelecom, H.
Organoids from Human Tooth Showing Epithelial Stemness Phenotype and Differentiation Potential. ,
Cell Mol Life Sci
 79 , 153 , 2022
Cells
Epithelial organoids
Enzymes
Medium
MEM
Organoids
Human

Human

Schröter, M. , Wang, C. , Terrigno, M. , Hornauer, P. , Huang, Z. , Jagasia, R. and Hierlemann, A.
Functional Imaging of Brain Organoids Using High-Density Microelectrode Arrays. , MRS Bull Vol. 47 , 530-544 , 2022
Cells
Cerebral organoids
Enzymes
PDS kit : see reference
Medium
PBS
Organoids
Human

Human

Uzquiano, A. , Kedaigle, A. , Pigoni, M. , Paulsen, B. , Adiconis, X. , Kim, K. , Faits, T. , Nagaraja, S. , Antón-Bolaños, N. , Gerhardinger, C. , Tucewicz, A. , Murray, E. , Jin, X. , Buenrostro, J. , Chen, F. , Velasco, S. , Regev, A. , Levin, J. and Arlotta, P.
Proper Acquisition of Cell Class Identity in Organoids Allows Definition of Fate Specification Programs of the Human Cerebral Cortex ,
Cell
 185 , 3770-3788.e27 , 2022
Cells
Brain organoids
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Xue, Y. , Seiler, M. , Tang, W. , Wang, J. , Delgado, J. , McLelland, B. , Nistor, G. , Keirstead, H. and Browne, A.
Retinal Organoids On-a-Chip: a Micro-Millifluidic Bioreactor for Long-term Organoid Maintenance. ,
Lab Chip
 21 , 3361-3377 , 2021
Cells
Stem cell-derived retinal organoids
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Vaart, J. , Böttinger, L. , Geurts, M. , Wetering, W. , Knoops, K. , Sachs, N. , Begthel, H. , Korving, J. , Lopez-Iglesias, C. , Peters, P. , Eitan, K. , Gileles-Hillel, A. and Clevers, H.
Modelling of Primary Ciliary Dyskinesia using Patient-derived Airway Organoids. , EMBO Rep Vol. 22 , e52058 , 2021
Cells
Airway organoids
Enzymes
Pronase : 0.15%
Collagenase : 0.05%
Medium
Airway medium
Organoids
Human

Human

Marinucci, M. , Ercan, C. , Taha-Mehlitz, S. , Fourie, L. , Panebianco, F. , Bianco, G. , Gallon, J. , Staubli, S. , Soysal, S. , Zettl, A. , Rauthe, S. , Vosbeck, J. , Droeser, R. , Bolli, M. , Peterli, R. , Flüe, M. , Ng, C. , Kollmar, O. , Coto-Llerena, M. and Piscuoglio, S.
Standardizing Patient-Derived Organoid Generation Workflow to Avoid Microbial Contamination From Colorectal Cancer Tissues. , Front Oncol Vol. 11 , 781833 , 2021
Cells
Colorectal cancer organoids
Enzymes
Medium
DMEM/F-12
Organoids
Human

Human

Schreurs, R. , Baumdick, M. , Drewniak, A. and Bunders, M.
Co-culture of Human Intestinal Organoids and Lamina Propria-derived CD4 T Cells. , STAR Protoc Vol. 2 , 100519 , 2021
Cells
Lamina propria lymphocytes
Enzymes
Medium
DMEM/F-12
Organoids
Human

Human

Barbar, L. , Rusielewicz, T. , Zimmer, M. , Kalpana, K. and Fossati, V.
Isolation of Human CD49f Astrocytes and iPSC-Based Neurotoxicity Assays. , STAR Protoc Vol. 1 , 100172 , 2020
Cells
Astrocytes
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Velasco, S. , Kedaigle, A. , Simmons, S. , Nash, A. , Rocha, M. , Quadrato, G. , Paulsen, B. , Nguyen, L. , Adiconis, X. , Regev, A. , Levin, J. and Arlotta, P.
Individual Brain Organoids Reproducibly Form Cell Diversity of the Human Cerebral Cortex. ,
Nature
 570 , 523-527 , 2019
Cells
Brain organoids
Enzymes
PDS kit : see reference
Medium
PBS
Organoids
Human

Human

Mansour, A. , Gonçalves, J. , Bloyd, C. , Li, H. , Fernandes, S. , Quang, D. , Johnston, S. , Parylak, S. , Jin, X. and Gage, F.
An In Vivo Model of Functional and Vascularized Human Brain Organoids. ,
Nat Biotechnol
 36 , 432-441 , 2018
Cells
Cerebral organoids
Enzymes
Medium
DMEM/F12
Organoids
Human

Human, embryonic

Nikolić, M. , Caritg, O. , Jeng, Q. , Johnson, J. , Sun, D. , Howell, K. , Brady, J. , Laresgoiti, U. , Allen, G. , Butler, R. , Zilbauer, M. , Giangreco, A. and Rawlins, E.
Human Embryonic Lung Epithelial Tips are Multipotent Progenitors that can be Expanded In vitro as Long-term Self-renewing Organoids. ,
Elife
 6 , 2017
Cells
Lung organoids
Enzymes
Medium
DMEM/F12
Reproductive
Human
Human
Chen, J. and Roan, N.
Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts. ,
Bio Protoc
 5 , 2015
Cells
Endometrial epithelial and stromal fibroblasts
Enzymes
Hyaluronidase : 62 u/ml
Medium
DMEM
Reproductive
Human
Human
Evans, J. and Salamonsen, L.
Decidualized Human Endometrial Stromal Cells are Sensors of Hormone Withdrawal in the Menstrual Inflammatory Cascade. ,
Biol Reprod
 90 , 14 , 2014
Cells
Endometrial stromal
Enzymes
Medium
DMEM/F-12
Reproductive
Human
Human
Patel, J. , Seppanen, E. , Chong, M. , Yeo, J. , Teo, E. , Chan, J. , Fisk, N. and Khosrotehrani, K.
Prospective Surface Marker-Based Isolation and Expansion of Fetal Endothelial Colony-Forming Cells from Human Term Placenta. ,
Stem Cells Transl Med
 2 , 839-47 , 2013
Cells
Endothelial colony forming
Enzymes
Medium
HBSS
Reproductive
Human
Human
Patel, M. , Ghosh, M. , Fahey, J. and Wira, C.
Uterine Epithelial Cells Specifically Induce Interferon-Stimulated Genes in Response to Polyinosinic-Polycytidylic Acid Independently of Estradiol. ,
PLoS ONE
 7 , e35654 , 2012
Cells
Uterine epithelial
Enzymes
Pancreatin : 0.34%
Hyaluronidase : 0.01%
Collagenase : 0.16%
Medium
HBSS
Reproductive
Human
Human
Shalini, V. , Pratheep, S. , Muhammad, A. , Sharmili, V. , Elizabeth, G. and Rajesh, R.
Generation and Characterisation of Human Mesenchymal Stem Cells Derived From Umbilical Cord and Placenta ,
Regenerative Research
 1 , 48 , 2012
Cells
Mesenchymal stem
Enzymes
Medium
DMEM/F12
Reproductive
Human
Human
Steigman, S. and Fauza, D.
Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta. ,
Curr Protoc Stem Cell Biol
 Chapter 1 , Unit 1E.2 , 2007
Cells
Mesenchymal stem
Enzymes
Neutral Protease : see reference
Medium
CMF-DPBS
Reproductive
Human
Human, female
Lockwood, C. , Arcuri, F. , Toti, P. , Felice, C. , Krikun, G. , Guller, S. , Buchwalder, L. and Schatz, F.
Tumor Necrosis Factor-Alpha and Interleukin-1 Beta Regulate Interleukin-8 Expression in Third Trimester Decidual Cells: Implications for the Genesis of Chorioamnionitis. ,
Am J Pathol
 169 , 1294-302 , 2006
Cells
Decidual
Enzymes
Collagenase : 0.25%
Deoxyribonuclease I : 6.25 u/ml
Medium
DMEM/F12
Reproductive
Human
Human
Spessotto, P. , Bulla, R. , Danussi, C. , Radillo, O. , Cervi, M. , Monami, G. , Bossi, F. , Tedesco, F. , Doliana, R. and Colombatti, A.
EMILIN1 Represents a Major Stromal Element Determining Human Trophoblast Invasion of the Uterine Wall. ,
J Cell Sci
 119 , 4574 , 2006
Cells
Extravillanous trophoblasts and decidual stromal
Enzymes
Trypsin : 0.1-0.25%
Deoxyribonuclease I : 0.02-0.05%
Medium
HBSS RPMI
Reproductive
Human
Human, female
Meter, R. , Wira, C. and Fahey, J.
Secretion of Monocyte Chemotactic Protein-1 by Human Uterine Epithelium Directs Monocyte Migration in Culture. ,
Fertil Steril
 84 , 191 , 2005
Cells
Uterine epithelial
Enzymes
Pancreatin : 0.34%
Hyaluronidase : 0.01%
Collagenase : 0.16%
Medium
HBSS
Reproductive
Human
Human
Teng Yan, Xue Wu-jun, Ding Xiao-ming, Feng Xin-shun, Xiang He-li, Jiang Ya-zhuo, Tian Pu-xun.
Isolation and culture of adult Sertoli cells and their effects on the function of co-cultured allogeneic islets in vitro ,
Chin Med J (Engl)
 118 , 1857-62 , 2005
Cells
Sertoli cells
Enzymes
Medium
DMEM/F-12
Reproductive
Human
Human
Wang Xin, Athayde Neil, Trudinger Brian.
Microvascular endothelial cell activation is present in the umbilical placental microcirculation in fetal placental vascular disease ,
Am J Obstet Gynecol
 190 , 596-601 , 2004
Cells
Endothelial placental
Enzymes
Medium
DMEM
Reproductive
Human
Human, female, 34-51 yr
Chan Rachel W S, Schwab Kjiana E, Gargett Caroline E.
Clonogenicity of human endometrial epithelial and stromal cells ,
Biol Reprod
 70 , 1738-50 , 2004
Cells
Endometrial epithelial and stromal cells
Enzymes
Medium
DMEM/F-12
Reproductive
Human
Human
Sun Kang, Myatt Leslie.
Enhancement of glucocorticoid-induced 11beta-hydroxysteroid dehydrogenase type 1 expression by proinflammatory cytokines in cultured human amnion fibroblasts ,
Endocrinology
 144 , 5568-77 , 2003
Cells
Amnion epithelial and fibroblast
Enzymes
Trypsin : 0.125%
Collagenase : 0.1%
Medium
PBS
Reproductive
Human
Human
Fahey John V, Wira Charles R.
Effect of menstrual status on antibacterial activity and secretory leukocyte protease inhibitor production by human uterine epithelial cells in culture ,
J Infect Dis
 185 , 1606-13 , 2002
Cells
Uterine epithelial cells
Enzymes
Pancreatin : 0.34%
Collagenase : 0.16%
Hyaluronidase : 0.16%
Medium
HBSS
Reproductive
Human
Human, embryo
Oberlin, E. , Tavian, M. , Blazsek, I. and Peault, B.
Blood-forming potential of vascular endothelium in the human embryo ,
Development
 129 , 4147 , 2002
Cells
Endothelial
Hematopoietic
Stromal
Enzymes
Medium
DMEM
Reproductive
Human
Human, female
Witz Craig A, Allsup Karen T, Montoya-Rodriguez Iris A, Vaughn Shelby L, Centonze Victoria E, Schenken Robert S.
Culture of menstrual endometrium with peritoneal explants and mesothelial monolayers confirms attachment to intact mesothelial cells ,
Hum Reprod
 17 , 2832-8 , 2002
Cells
Mesothelial
Enzymes
Medium
Eagle's MEM
Reproductive
Human
Human
Zhang, S. , Wernig, M. , Duncan, I. , Brustle, O. and Thomson, J.
In Vitro Differentiation of Transplantable Neural Precursors from Human Embryonic Stem Cells ,
Nat Biotechnol
 19 , 1129 , 2001
Cells
Stem, embryonic
Enzymes
Neutral Protease : 0.01% - 0.02%
Medium
DMEM
Reproductive
Human
Human, 20-40 yr
Arnold, J. , Kaufman, D. , Seppala, M. and Lessey, B.
Endometrial stromal cells regulate epithelial cell grownth in vitro: a new co-culture model ,
Hum Reprod
 16 , 836 , 2001
Cells
Endometrium epithelial and stromal cells
Enzymes
Medium
HBSS
Reproductive
Human
Human
Yusuf RZ, Naeem R.
Cytogenetic studies of spontaneous miscarriages: a seven year study to compare significance of primary vs. secondary culture methods for assessment of fetal karyotype yield and maternal cell contamination ,
Early Pregnancy
 5 , 121-31 , 2001
Cells
Chorionic villi
Enzymes
Trypsin : see reference
Collagenase Type 3 : 100 u/ml
Medium
HBSS
Reproductive
Human
Human, female, 20-40 year
Gargett CE, Bucak K, Rogers PA.
Isolation, characterization and long-term culture of human myometrial microvascular endothelial cells ,
Hum Reprod
 15 , 293-301 , 2000
Cells
Microvascular endothelial cells
Enzymes
Medium
PBS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.