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Tissue References Search Tool

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Pancreas
Mouse
Mouse
Li, D. , Yuan, Y. , Tu, H. , Liang, Q. and Dai, L.
A Protocol for Islet Isolation from Mouse Pancreas. ,
Nat Protoc
 4 , 1649 , 2009
Cells
Islets
Enzymes
Collagenase : 1,000 u/ml
Medium
RPMI 1640
Pancreas
Mouse
Mouse
Carter, J. , Dula, S. , Corbin, K. , Wu, R. and Nunemaker, C.
A Practical Guide to Rodent Islet Isolation and Assessment. ,
Biol Proced Online
 11 , 3 , 2009
Cells
Islets
Enzymes
Collagenase : 0.14%
Medium
RPMI 1640
Pancreas
Mouse
Mouse, adult, male
Ji, B. , Gaiser, S. , Chen, X. , Ernst, S. and Logsdon, C.
Intracellular Trypsin Induces Pancreatic Acinar Cell Death but not NF-KappaB Activation. ,
J Biol Chem
 284 , 17488 , 2009
Cells
Ancinar
Enzymes
CLSPA : see reference
Medium
DMEM
Pancreas
Mouse
Mouse, 3-4 week, 20-24 g
Huang, H. , Xie, Q. , Kang, M. , Zhang, B. , Zhang, H. , Chen, J. , Zhai, C. , Yang, D. , Jiang, B. and Wu, Y.
Labeling Transplanted Mice Islet with Polyvinylpyrrolidone Coated Superparamagnetic Iron Oxide Nanoparticles for In Vivo Detection By Magnetic Resonance Imaging. ,
Nanotechnology
 20 , 365101 , 2009
Cells
Islets
Enzymes
Medium
RPMI 1540
Pancreas
Mouse
Mouse
Szot, G. , Koudria, P. and Bluestone, J.
Murine Pancreatic Islet Isolation. ,
J Vis Exp
 7 , 255 , 2007
Cells
Islets
Enzymes
Collagenase : 0.03-0.08%
Medium
RPMI 1640
Pancreas
Mouse
Mouse, 6-10 month
Wang, Y. , Soyombo, A. , Shcheynikov, N. , Zeng, W. , Dorwart, M. , Marino, C. , Thomas, P. and Muallem, S.
Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis. ,
EMBO J
 25 , 5049 , 2006
Cells
Pancreatic ductal
Enzymes
CLSPA : 50 u/ml
Hyaluronidase : 400 u/ml
Medium
DMEM
Pancreas
Mouse
Mouse, adult
Voronina, S. , Barrow, S. , Gerasimenko, O. , Petersen, O. and Tepikin, A.
Effects of Secretagogues and Bile Acids on Mitochondrial Membrane Potential of Pancreatic Acinar Cells: Comparison of Different Modes of Evaluating Delta Psi. ,
J Biol Chem
 279 , 27327 , 2004
Cells
Acinar
Enzymes
CLSPA : 200 u/ml
Medium
see reference
Pancreas
Mouse
Mouse, 11-12 week
Astrof, S. , Crowley, D. , George, E. , Fukuda, T. , Sekiguchi, K. , Hanahan, D. and Hynes, R.
Direct Test of Potential Roles of EIIIA and EIIIB Alternatively Spliced Segments of Fibronectin in Physiological and Tumor Angiogenesis. ,
Mol Cell Biol
 24 , 8662-70 , 2004
Cells
Islets
Enzymes
Medium
RPMI
Pancreas
Mouse
Mouse
Wu Yulian, Han Bing, Luo Hongyu, Roduit Raphael, Salcedo TheodoraW, Moore PaulA, Zhang Jun, Wu Jiangping.
DcR3/TR6 effectively prevents islet primary nonfunction after transplantation ,
Diabetes
 52 , 2279-86 , 2003
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Mouse
Mouse
Haefliger Jacques-Antoine, Tawadros Thomas, Meylan Laure, Gurun SabineLe, Roehrich Marc-Estienne, Martin David, Thorens Bernard, Waeber Gerard.
The scaffold protein IB1/JIP-1 is a critical mediator of cytokine-induced apoptosis in pancreatic beta cells ,
J Cell Sci
 116 , 1463-9 , 2003
Cells
Pancreatic islet
Enzymes
Medium
HBSS
Pancreas
Mouse
Mouse, BALB/c, 6-8 week-old, either sex
Kurup, S. and Bhonde, R.
Analysis and Optimization of Nutritional Set-up for Murine Pancreatic Acinar Cells ,
JOP
 3 (1) , 8 , 2002
Cells
Acinar
Enzymes
Collagenase : 0.1%
Medium
Waymouth's MB
Pancreas
Mouse
Mouse, albino, 25 g
Fogarty, K. , Kidd, J. , Tuft, R. and Thorn, P.
A Bimodal Pattern of InsP(3)-Evoked Elementary Ca(2+) Signals in Pancreatic Acinar Cells. ,
Biophys J
 78 , 2298 , 2000
Cells
Acinar
Enzymes
CLSPA : see reference
Medium
see reference
Pancreas
Mouse
Mouse
Strowski M, Parmar R, Blake A, Schaeffer J.
Somatostatin inhibits insulin and glucagon secretion via two receptors subtypes: an in vitro study of pancreatic islets from somatostatin receptor 2 knockout mice ,
Endocrinology
 141 , 111-7 , 2000
Cells
Pancreatic islets
Enzymes
Medium
Gey's BSS
Pancreas
Mouse
Mouse
Koster, J. , Marshall, B. , Ensor, N. , Corbett, J. and Nichols, C.
Targeted Overativity of Cell KATP Channels Induces Profound Neonatal Diabetes ,
Cell
 100 , 645 , 2000
Cells
Islets
Enzymes
Medium
CF Medium
Pancreas
Mouse
Mouse
Toivola, D. , Ku, N. , Ghori, N. , Lowe, A. , Michie, S. and Omary, M.
Effects of Keratin Filament Disruption on Exocrine Pancreas-Stimulated Secretion and Susceptibility to Injury. ,
Exp Cell Res
 255 , 156 , 2000
Cells
Acinar cells and acini
Enzymes
Collagenase Type 1 : see reference
CLSPA : see reference
Medium
see reference
Pancreas
Mouse
Mouse
Githens, S.
Pancreatic Duct Epithelial Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:12.1 , 1995
Cells
Duct
Enzymes
Papain : 25 u/ml
Medium
DMEM /F-12
Pancreas
Mouse
Mouse
Jauch, P. , Peterson, O. and Lauger, P.
Electrogenic Properties of the Na-Alanine Cotransporter in Acinar Cells ,
J Membr Biol
 94 , 99 , 1986
Cells
Acinar
Enzymes
Collagenase : 100 u/ml
Medium
HEPES
Pancreas
Mouse
Mouse (C57BL/6J-ob/ob), 9-12 wks, male
Dalpe-Scott, M. , Heick, H. and Begin-Heick, N.
Secretion in the Obese (ob/ob) Mouse. The Effect of Oxytetracycline on Insulin Release ,
Diabetes
 32 , 932 , 1983
Cells
Islets
Enzymes
Hyaluronidase : 0.5%
Medium
Kreb's Ringer bicarbonate buffer
Pancreas
Mouse
Mouse, male, 18-24 g
Burnham DB, Williams JA.
Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini ,
J Biol Chem
 257 , 10523-8 , 1982
Cells
Acinar
Enzymes
CLSPA : 70-90 u/ml
Medium
Krebs-Henseleit
Pancreas
Mouse
Mouse, 7-10 week
Yesil, P, Michel, M. , Chwalek, K. , Pedack, S. , Jany, C. , Ludwig, B. , Bornstein, S. and Lammert, E.
A New Collagenase Blend Increases the Number of Islets Isolated from Mouse Pancreas. ,
Islets
 1 , 185
Cells
Islets
Enzymes
Collagenase : see reference
Medium
DMEM
Parotid
Mouse
Mouse
Ogawa, M. , Oshima, M. , Imamura, A. , Sekine, Y. , Ishida, K. , Yamashita, K. , Nakajima, K. , Hirayama, M. , Tachikawa, T. and Tsuji, T.
Functional Salivary Gland Regeneration by Transplantation of a Bioengineered Organ Germ. ,
Nat Commun
 4 , 2498 , 2013
Cells
Parotid and sublingual glandular
Enzymes
Neutral Protease : 50 u/ml
Collagenase Type 1 : 100 u/ml
Medium
PBS
Pituitary
Mouse
Mouse
Perez Millain, M. , Brinkmeier, M. , Mortensen, A. and Camper, S.
PROP1 Triggers Epithelial-Mesenchymal Transition-Like Process in Pituitary Stem Cells. ,
Elife
 5 , 2016
Cells
Pituitary
Enzymes
Medium
HBSS
Pituitary
Mouse
Mouse
Pyczek, J. , Buslei, R. , Schult, D. , Holsken, A. , Buchfelder, M. , Hess, I. , Hahn, H. and Uhmann, A.
Hedgehog Signaling Activation Induces Stem Cell Proliferation and Hormone Release in the Adult Pituitary Gland. ,
Sci Rep
 6 , 24928 , 2016
Cells
Pituitary
Enzymes
Medium
DMEM
Pituitary
Mouse
Mouse, male
Steveson Tami C, Ciccotosto Giuseppe D, Ma Xin-Ming, Mueller Gregory P, Mains Richard E, Eipper Betty A.
Menkes protein contributes to the function of peptidylglycine alpha-amidating monooxygenase ,
Endocrinology
 144 , 188-200 , 2003
Cells
Pituitary
Enzymes
Collagenase : 0.4%
Hyaluronidase : 0.1%
Trypsin : 0.3%
Medium
DMEM/Han's F12
Prostate
Mouse
Mouse, 6-8 week
Burger, P. , Gupta, R. , Xiong, X. , Ontiveros, C. , Salm, S. , Moscatelli, D. and Wilson, E.
High Aldehyde Dehydrogenase Activity: A Novel Functional Marker of Murine Prostate Stem/Progenitor Cells. ,
Stem Cells
 27 , 2220-8 , 2009
Cells
Prostate epithelial/stem
Enzymes
Trypsin : 0.05%
Medium
HBSS
Prostate
Mouse
Mouse, 2 week
Imamov Otabek, Morani Andrea, Shim Gil-Jin, Omoto Yoko, Thulin-Andersson Christina, Warner Margaret, Gustafsson Jan-Ake.
Estrogen receptor beta regulates epithelial cellular differentiation in the mouse ventral prostate ,
Proc Natl Acad Sci U S A
 101 , 9375-80 , 2004
Cells
Prostatic epithelial
Enzymes
Collagenase Type 3 : 170 u/ml
Medium
DMEM
Prostate
Mouse
Mouse, male
Dubey P, Wu H, Reiter RE, Witte ON.
Alternative pathways to prostate carcinoma activate prostate stem cell antigen expression ,
Cancer Res
 61 , 3256-61 , 2001
Cells
Prostatic stem
Enzymes
Collagenase Type 1 : 170 u/ml
Medium
DMEM
Reproductive
Mouse
Mouse, 4 week
Hou, M. , Han, J. , Li, G. , Kwon, M. , Jiang, J. , Emani, S. , Taglauer, E. , Park, J. , Choi, E. , Vodnala, M. , Fong, Y. , Emani, S. , Rosas, I. , Perrella, M. and Liu, X.
Multipotency of Mouse Trophoblast Stem Cells ,
Stem Cell Res Ther
 11 , 55 , 2020
Cells
Trophoblast
Enzymes
Medium
DMEM
Reproductive
Mouse
Mouse, adult
Lee, A, Rusch, J. , Lima, A. , Usmani, A. , Huang, N. , Lepamets, M. , Vigh-Conrad, K. , Worthington, R. and et al.
Rare Mutations in the Complement Regulatory Gene CSMD1 are Associated with Male and Female Infertility. ,
Nat Commun
 10 , 4626 , 2019
Cells
Testes
Enzymes
Medium
MEM
Reproductive
Mouse
Mouse, 1-7 day
Kang, H. , Chen, L. , Lichti-Kaiser, K. , Liao, G. , Gerrish, K. , Bortner, C. , Yao, Hu. , Eddy, E. and Jetten, A.
Transcription Factor GLIS3: A New and Critical Regulator of Postnatal Stages of Mouse Spermatogenesis. ,
Stem Cells
 34 , 2772-2783 , 2016
Cells
Testicular
Enzymes
Medium
HBSS
Reproductive
Mouse
Mouse, adult
Martin, L. and Seandel, M.
Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-Term Adult Mouse SSC Lines. ,
J Vis Exp
 72 , e50017 , 2013
Cells
Spermatogonial stem
Enzymes
Trypsin : 0.05%
Medium
DMEM
Reproductive
Mouse
Mouse, female
Jiang, J. and Kelly, K.
Isolation of Lymphocytes from Mouse Genital Tract Mucosa. ,
J Vis Exp
 67 , e4391 , 2012
Cells
Lymphocytes
Enzymes
Collagenase : 450 u/ml
Medium
RPMI
Reproductive
Mouse
Mouse
White, Y. , Woods, D. , Takai, Y. , Ishihara, O. , Seki, H. and Tilly, J.
Oocyte Formation by Mitotically Active Germ Cells Purified from Ovaries of Reproductive-Age Aomen. ,
Nat Med
 18 , 413 , 2012
Cells
Ovarian
Enzymes
Collagenase Type 4 : 800 u/ml
Medium
HBSS
Reproductive
Mouse
Mouse
Venkataraman, L. , Lenis, A. , Couri, B. , Damaser, M. and Ramarurthi, A
Induced Regenerative Elastic Matrix Repair in LOXL 1 Knockout Mouse Cell Cultures: Towards Potential Therapy for Pelvic Organ Prolapse ,
J Tissue Sci Eng
 3 , 3 , 2012
Cells
Vaginal smooth muscle
Enzymes
Collagenase Type 2 : 175 u/ml
Elastase : 0.025%
Medium
DMEM/F12
Reproductive
Mouse
Mouse
Yamatoya, K. , Ito, C. , Araki, M. , Furuse, R. and Toshimori, K.
One-Step Collagenase Method for Zona Pellucida Removal in Unfertilized Eggs: Easy and Gentle Method for Large-Scale Preparation. ,
Reprod Med Bio
 10 , 97-103 , 2011
Cells
Zona pellucid free eggs
Enzymes
Collagenase : 0.1%
Medium
THY-HEPES
Reproductive
Mouse
Mouse, male
Getun, I. , Torres, B. and Bois, P.
Flow Cytometry Purification of Mouse Meiotic Cells. ,
J Vis Exp
 50 , 2602 , 2011
Cells
Testis, meiotic
Enzymes
Collagenase Type 1 : 120 u/ml
Trypsin : 0.1%
Medium
Gey's BSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.