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Adipose/Fat
Mouse

Mouse, Adult

Hay, A. , Murphy, T. , Popichak, K. , Zabel, M. and Moreno, J.
Adipose-derived mesenchymal stromal cells decrease prion-induced glial inflammation in vitro. ,
Sci Rep
 12 , 22567 , 2022
Cells
Mesenchymal stromal
Enzymes
Stemxyme 1 : 400u/ml
Medium
DMEM/F12
Adipose/Fat
Mouse
Mouse
Jones, J. , Rabhi, N. , Orofino, J. , Gamini, R. , Perissi, V. , Vernochet, C. and Farmer, S.
The Adipocyte Acquires a Fibroblast-Like Transcriptional Signature in Response to a High Fat Diet. ,
Sci Rep
 10 , 2380 , 2020
Cells
Adipocytes
Enzymes
Medium
DMEM
Adipose/Fat
Mouse
Mouse
Bowles, A. , Strong, A. , Wise, R. , Thomas, R. , Gerstein, B. , Dutreil, M. , Hunter, R. , Gimble, J. and Bunnell, B.
Adipose Stromal Vascular Fraction-Mediated Improvements at Late-Stage Disease in a Murine Model of Multiple Sclerosis. ,
Stem Cells
 35 , 532-544 , 2017
Cells
Adipose stromal
Enzymes
Medium
HBSS
Adipose/Fat
Mouse
Mouse
Yao, W. , Lay, Y. , Kot, A. , Liu, R. , Zhang, H. , Chen, H. , Lam, K. and Lane, N.
Improved Mobilization of Exogenous Mesenchymal Stem Cells to Bone for Fracture Healing and Sex Difference. ,
Stem Cells
 34 , 2587-2600 , 2016
Cells
Adipose stromal
Enzymes
Medium
PBS
Adipose/Fat
Mouse
Mouse
Maria, O. , Shalaby, M. , Syme, A. , Eliopoulos, N. and Muanza, T.
Adipose Mesenchymal Stromal Cells Minimize and Repair Radiation-Induced Oral Mucositis. ,
Cytotherapy
 18 , 1129-45 , 2016
Cells
Adipose mesenchyal stromal
Enzymes
Medium
DMEM
Adipose/Fat
Mouse
Mouse
Jang, H. , Bhasin, S. , Guarneri, T. , Serra, C. , Schneider, M. , Lee, M. , Guo, W. , Fried, S. , Pencina, K. and Jasuja, R.
The Effects of A Single Developmentally-Entrained Pulse of Testosterone in Female Neonatal Mice On Reproductive and Metabolic Functions in Adult Life. ,
Endocrinology
 156 , 3737 , 2015
Cells
Adipocytes
Enzymes
Collagenase : 0.1%
Medium
Krebs
Adipose/Fat
Mouse
Mouse
Seaman, S. , Tannan, S. , Cao, Y. , Peirce, S. and Lin, K.
Differential Effects of Processing Time and Duration of Collagenase Digestion on Human and Murine Fat Grafts. ,
Plast Reconstr Surg
 136 , 189e-199e , 2015
Cells
Adipocytes
Enzymes
Medium
PBS
Adipose/Fat
Mouse
Mouse, 20 week
Kondo, T, Toyoshima, Y. , Ishii, Y. and Kyuwa, S.
Natural Killer T Cells in Adipose Tissue are Activated in Lean Mice. ,
Exp Anim
 62 , 319 , 2013
Cells
Stromal vascular
Enzymes
Medium
RPMI-1640
Adipose/Fat
Mouse
Mouse
Takahashi, H. , Haraguchi, N. , Nishikawa, S. , Miyazaki, S. , Suzuki, Y. , Mizushima, T. , Nishimura, J. , Takemasa, I. , Yamamoto, H. , Mimori, K. , Ishii, H. , Doki, Y. and Mori, M.
Biological and Clinical Availability of Adipose-Derived Stem Cells for Pelvic Dead Space Repair. ,
Stem Cells Transl Med
 1 , 803 , 2012
Cells
Adipose derived stem
Enzymes
Medium
DMEM
Adipose/Fat
Mouse
Mouse
Han, J. , Koh, Y. , Moon, H. , Ryoo, H. , Cho, C. , Kim, I. and Koh, G.
Adipose Tissue is an Extramedullary Reservoir for Functional Hematopoietic Stem and Progenitor Cells. ,
Blood
 115 , 957 , 2010
Cells
Stem and progenitor
Enzymes
Medium
HBSS
Adipose/Fat
Mouse
Mouse
Wong, K. , Szeto, F, Zhang, W. , Ye, H. , Kong, J. , Zhang, Z. , Sun, X. and Li, Y
Involvement of the Vitamin D Receptor in Energy Metabolism: Regulation of Uncoupling Proteins. ,
Am J Physiol/Endo
 296 , 820 , 2009
Cells
White adipocytes
Enzymes
Medium
DMEM
Adipose/Fat
Mouse
Mouse
Thangarajah, H. , Vial, I. , Chang, E. , El-Ftesi, S. , Januszyk, M. , Chang, E. , Paterno, J. , Neofytou, E. , Longaker, M. and Gurtner, G.
IFATS Collection: Adipose Stromal Cells Adopt a Proangiogenic Phenotype Under the Influence of Hypoxia. ,
Stem Cells
 27 , 266 , 2009
Cells
Adipose stromal
Enzymes
Medium
DMEM
Adipose/Fat
Mouse
Mouse, 3 week
De Matteis, R. , Zingaretti, M. , Murano, I. , Vitali, A. , Frontini, A. , Giannulis, I. , Barbatelli, G. , Marcucci, F. , Bordicchia, M. , Sarzani, R. , Raviola, E. and Cinti, S.
In Vivo Physiological Transdifferentiation of Adult Adipose Cells. ,
Stem Cells
 27 , 2761 , 2009
Cells
Adipocytes
Enzymes
Medium
HBSS
Adipose/Fat
Mouse
Mouse
Cho, K. , Park, H. , Park, H. , Jung, J. , Jeon, S. , Kim, Y. and Roh, H.
IFATS Collection: Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells in an Allergic Rhinitis Mouse Model. ,
Stem Cells
 27 , 259-65 , 2009
Cells
Adipose tissue-derived stem
Enzymes
Medium
Modified Eagles
Adipose/Fat
Mouse
Mouse, C57Bl/6J
Aoyagi T, Shimba S, Tezuka M.
Characteristics of Circadian Gene Expressions in Mice White Adipose Tissue and 3T3-L1 Adipocytes ,
J Health Sci
 51 , 21 , 2005
Cells
Adipocytes
Enzymes
Medium
DMEM/F12
Adipose/Fat
Mouse
Mouse
Planat-Benard Valerie, Silvestre Jean-Sebastien, Cousin Beatrice, Andre Mireille, Nibbelink Maryse, Tamarat Radia, Clergue Michel, Manneville Carole, Saillan-Barreau Corinne, Duriez Micheline, Tedgui Alain, Levy Bernard, Penicaud Luc, Casteilla Louis.
Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives ,
Circulation
 109 , 656-63 , 2004
Cells
Stromal vascular, adipocytes
Enzymes
Collagenase : 0.2%
Medium
DMEM/F12
Adipose/Fat
Mouse
Mouse, 3-6 day and 2-3 month
Cowan Catherine M, Shi Yun-Ying, Aalami Oliver O, Chou Yu-Fen, Mari Carina, Thomas Romy, Quarto Natalina, Contag Christopher H, Wu Benjamin, Longaker Michael T.
Adipose-derived adult stromal cells heal critical-size mouse calvarial defects ,
Nat Biotechnol
 22 , 560-7 , 2004
Cells
Adipose-derived stromal cells
Enzymes
Medium
PBS
Adipose/Fat
Mouse
Mouse, C57BL/6J and FVB, male, 8-9 week
Ruan, H. , Zarnowski, MJ. , Cushman, S. and Lodish, H.
Standared Isolation of Primary Adipose Cells from Mouse Epididymal Fat Pads Induces Inflammatory Mediators and Down-regulates Adipocyte Genes ,
J Biol Chem
 278 , 47585 , 2003
Cells
Adipocytes
Enzymes
Collagenase : see reference
Medium
DMEM
Adipose/Fat
Mouse
Mouse, both sexes
Nadler, S. , Stoehr, J. , Rabaglia, M. , Schueler, K. , Birnbaum, M. and Attie, A.
Normal Akt/PKB with Reduced PI3K Activation in Insulin-resistant Mice ,
Am J Physiol/Endo
 281 , E1249 , 2001
Cells
Adipocytes
Enzymes
Collagenase : 0.05%
Medium
Krebs-Ringer Phosphate HEPES (KRPH)
Adipose/Fat
Mouse
Mouse, B6D2F1, F1 hybrids, New Zealand black female & New Zealand white male
Launder, T. , Gegen, N. , Knedler, A. and Harbeck, R.
The Isolation and Characterization of Enriched Microvascular Endothelial Cells From Mouse Adipose Tissue ,
J Immunol Methods
 102 , 45 , 1987
Cells
Vascular endothelial
Enzymes
Collagenase : 0.2%
Medium
PBS
Endothelial
Mouse
Mouse, 4 week
Esteban, S. , Clemente, C. , Koziol, A. , Gonzalo, P. , Rius, C. , Martinez, F. , Linares, P. , Chaparro, M. , Urzainqui, A. , Andres, V. , Seiki, M. , Gisbert, J. and Arroyo, A.
Endothelial MT1-MMP Targeting Limits Intussusceptive Angiogenesis and Colitis via TSP1/Nitric Oxide Axis. ,
EMBO Mol Med
 12 , e10862 , 2020
Cells
Aortic endothelial
Enzymes
Elastase : 0.25%
Medium
DMEM
Endothelial
Mouse
Mouse, 2 month
Sabbagh, M. and Nathans, J.
A Genome-wide View of the De-differentiation of Central Nervous System Endothelial Cells in Culture ,
Elife
 , 1 , 2020
Cells
Endothelial, CNS
Enzymes
PDS kit : with modifications
Stemxyme 1 : 0.1%
Medium
EC growth medium
Endothelial
Mouse
Mouse
Luo, S. , Truong, A. and Makino, A.
Isolation of Mouse Coronary Endothelial Cells. ,
J Vis Exp
 , 2016
Cells
Coronary endothelial
Enzymes
Medium
Kreb's
Endothelial
Mouse
Mouse
Wang, J. , Sun, C. , Gerdes, N. , Liu, C. , Liao, M. , Liu, J. , Shi, M. , He, A. , Zhou, Y. , Sukhova, G. , Chen, H. , Cheng, X. , Kuzuya, M. , Murohara, T. , Zhang, J. , Cheng, X. , Jiang, M. , Shull, G. and Rogers, S.
Interleukin 18 Function in Atherosclerosis is Mediated by the Interleukin 18 Receptor and the Na-Cl Co-Transporter. ,
Nat Med
 21 , 820-6 , 2015
Cells
Endothelial lung
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse
Xiao, L. , McCann, J. and Dudley, A.
Isolation and Culture Expansion of Tumor-specific Endothelial Cells. ,
J Vis Exp
 , e53072 , 2015
Cells
Endothelial
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse
Kochukov, M. , Balasubramanian, A. , Abramowitz, J. , Birnbaumer, L. and Marrelli, S.
Activation of Endothelial Transient Receptor Potential C3 Channel is Required for Small Conductance Calcium-Activated Potassium Channel Activation and Sustained Endothelial Hyperpolarization and Vasodilation of Cerebral Artery. ,
J Am Heart Assoc
 3 , 2014
Cells
Cerebral artery endothelial
Enzymes
Neutral Protease : 4 u/ml
Elastase : 1 u/ml
Collagenase Type 1 : 120 u/ml
Medium
DMEM
Endothelial
Mouse
Mouse
Kazerounian, S. , Gerald, D. , Huang, M. , Chin, R, Udayakumar, D, Zheng, N. , O'Donnell, R. , Perruzzi, C. , Mangiante, L. , Pourat, J. , Phung, T. , Bravo-Nuevo, A. , Shechter, S. , McNamara, S. and Duhadaway, K.
RhoB Differentially Controls Akt Function in Tumor Cells and Stromal Endothelial Cells During Breast Tumorigenesis. ,
Cancer Res
 73 , 50 , 2013
Cells
Tumor endothelial
Enzymes
Medium
HBSS
Endothelial
Mouse
Mouse, 8-14 week
Imoukhuede, P. and Popel, A.
Expression of VEGF Receptors on Endothelial Cells in Mouse Skeletal Muscle. ,
PLoS ONE
 7 , e44791 , 2012
Cells
Endothelial
Enzymes
Medium
HBSS
Endothelial
Mouse
Mouse
Daneman, R. , Zhou, L. , Agalliu, D. , Cahoy, J. , Kaushal, A. and Barres, B.
The Mouse Blood-Brain Barrier Transcriptome: a New Resource for Understanding the Development and Function of Brain Endothelial Cells. ,
PLoS ONE
 5 , e13741 , 2010
Cells
Endothelial
Enzymes
Papain : 40 u/ml
Deoxyribonuclease I : 125 u/ml
Medium
DPBS
Endothelial
Mouse
Mouse, 7-10 week
Follenzi, A. , Benten, D. , Novikoff, P. , Faulkner, L. , Raut, S. and Gupta, S.
Transplanted Endothelial Cells Repopulate the Liver Endothelium and Correct the Phenotype of Hemophilia A Mice. ,
J Clin Invest
 118 , 935 , 2008
Cells
Liver endothelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Endothelial
Mouse
Mouse, 4 week
Kondo, S. , Scheef, E. , Sheibani, N. and Sorenson, C.
PECAM-1 Isoform-Specific Regulation of Kidney Endothelial Cell Migration and Capillary Morphogenesis. ,
Am J Physiol Cell Physiol
 292 , C2070 , 2007
Cells
Endothelial kidney
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse, embryonic or yolk sac
Braren Rickmer, Hu Huiqing, Kim YungHae, Beggs HilaryE, Reichardt LouisF, Wang Rong.
Endothelial FAK is essential for vascular network stability, cell survival, and lamellipodial formation ,
J Cell Biol
 172 , 151-62 , 2006
Cells
Endothelial
Enzymes
Medium
PBS
Endothelial
Mouse
Mouse, neonatal
Cha, S. , Talavera, D. , Demir, E. , Nath, A. and Sierra-Honigmann, M.
A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin. ,
Microvasc Res
 70 , 198 , 2005
Cells
Microvascular endothelial
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse, 4 week
Su X, Sorenson CM. and Sheibani N.
Isolation and characterization of murine retinal endothelial cells ,
Mol Vis
 9 , 171 , 2003
Cells
Retinal endothelial cells
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse, male
Izawa D, Tanaka T, Saito K, Ogihara H, Usui T, Kawamoto S, Matsubara K, Okubo K. and Miyasaka M
Expression profile of active genes in mouse lymph node high endothelial cells ,
Int Immunol
 11(12) , 1989 , 1999
Cells
Endothelial cells from lymph node
Enzymes
Medium
PBS
Endothelial
Mouse
Mouse, 2-4 week
Sapatino, B. , Welsh, C. , Smith, C. , Bebo, B. and Linticum, D.
Cloned Mouse Cerebrovascular Endothelial Cells That Maintain Their Differentiation Markers for Factor VIII, Low Density Lipoprotein, and Angiotensin-Converting Enzyme ,
In Vitro Cell Dev Biol
 29A , 923 , 1993
Cells
Cerebrovascular Endothelial
Enzymes
Medium
PBS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.