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Tissue References Search Tool

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Bone
Mouse
Mouse
Mohrin, D. , Koukos, G. , Alabdulaaly, L. , Paw, J. , Hake, K. , Kolumam, G. , Bouxsein, M. , Baron, R. , Kutskova, Y. , Freund, A. , Liu, J. , Zavala-Solorio, J. , Bhargava, S. , Maxwell, Trumble, J. , Brito, A. , Hu, D. and Brooks, D.
Inhibition of Longevity Regulator PAPP-A Modulates Tissue Homeostasis via Restraint of Mesenchymal Stromal Cells. , Aging Cell Vol. 20 , e13313 , 2021
Cells
Bone and bone marrow stromal
Enzymes
Medium
PBS
Bone
Mouse
Mouse
Baryawno, N. , Przybylski, D. , Kowalczyk, M. , Kfoury, Y. , Severe, N. , Gustafsson, K. , Kokkaliaris, K. , Mercier, F. , Tabaka, M. , Hofree, M. , Dionne, D. , Papazian, A. , Lee, D. , Ashenberg, O. and Subramanian, A.
A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia. ,
Cell
 177 , 1915-1932.e16 , 2019
Cells
Bone and bone marrow stroma
Enzymes
Medium
Media 199
Bone
Mouse
Mouse
Wang, W. , Majihail, G. , Lui, C. and Zhou, L.
Osteoblast Sorting and Intracellular Staining of CXCL12. ,
Bio Protoc
 8 , 2018
Cells
Osteoblast
Enzymes
Medium
HBSS
Bone
Mouse
Mouse
Agarwal, S. , Loder, S. , Sorkin, M. , Li, S. , Shrestha, S. , Zhao, B. , Mishina, Y. , James, A. and Levi, B.
Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification. ,
Stem Cells
 34 , 1692-701 , 2016
Cells
Bone-cartilage-stromal
Enzymes
Medium
HBSS
Bone
Mouse
Mouse
Igarashi, H. , Akahoshi, N. , Ohto-Nakanishi, T. , Yasuda, D. and Ishii, S.
The Lysophosphatidic Acid Receptor LPA4 Regulates Hematopoiesis-Supporting Activity of Bone Marrow Stromal Cells. ,
Sci Rep
 5 , 11410 , 2015
Cells
Bone stromal
Enzymes
Medium
DMEM
Bone
Mouse
Mouse
Kode, A.,Manavalan1, J.,Mosialou1, I. , Bhagat G. and Rathinam, C, Luo, N et al.
Leukemogenesis Induced by an Activating β-catenin Mutation in Osteoblasts ,
Nature
 506 , 240 , 2014
Cells
Osteoblasts
Enzymes
Medium
MEM
Bone
Mouse
Mouse
Lee, D. , Choung, H. , Kim, H. , Gronostajski, R. , Yang, Y. , Ryoo, H. , Lee, Z. , Kim, H. , Cho, E. and Park, J.
NFI-C Regulates Osteoblast Differentiation Via Control of Osterix Expression. ,
Stem Cells
 32 , 2467-79 , 2014
Cells
Osteoblasts
Enzymes
Medium
MEM
Bone
Mouse
Mouse, 2-5 day
Taylor, S. , Shah, M. and Orriss, I.
Generation of Rodent and Human Osteoblasts. ,
Bonekey Rep
 3 , 585 , 2014
Cells
Osteoblasts
Enzymes
Trypsin : 0.25%-1.0%
Medium
DMEM
Bone
Mouse
Mouse, 4-22 month
Stern, A. , Stern, M. , Van Dyke, M. , Jahn, K. , Prideaux, M. and Bonewald, L.
Isolation and Culture of Primary Osteocytes from the Long Bones of Skeletally Mature and Aged Mice. ,
Biotechniques
 52 , 361 , 2012
Cells
Osteocytes
Enzymes
Collagenase Type 1 : 300 u/ml
Medium
MEM
Bone
Mouse
Mouse
Nakamura, Y. , Arai, F. , Iwasaki, H. , Hosokawa, K. , Kobayashi, I. , Gomei, Y. , Matsumoto, Y. , Yoshihara, H. and Suda, T.
Isolation and Characterization of Endosteal Niche Cell Populations that Regulate Hematopoietic Stem Cells. ,
Blood
 116 , 1422 , 2010
Cells
Endosteal cells
Enzymes
Medium
DMEM
Bone
Mouse
Mouse
Morikawa, S. , Mabuchi, Y. , Kubota, Y. , Nagai, Y. , Niibe, K. , Hiratsu, E. , Suzuki, S. , Miyauchi-Hara, C. , Nagoshi, N. , Sunabori, T. , Shimmura, S. , Miyawaki, A. , Nakagawa, T. , Suda, T. , Okano, H. and Matsuzaki, Y.
Prospective Identification, Isolation, and Systemic Transplantation of Multipotent Mesenchymal Stem Cells in Murine Bone Marrow. ,
J Exp Med
 206 , 2483-96 , 2009
Cells
Bone marrow
Enzymes
Collagenase : 0.2%
Medium
DMEM
Bone
Mouse
Mouse, male, 6-8 week old
Sakai, E. , Miyamoto, H. , Okamoto, K. , Kato, Y. , Yamamoto, K. and Sakai, H.
Characterization of Phagosomal Subpopulations Along Endocytic Routes in Osteoclasts and Macrophages ,
J. Biochem.
 130 , 823 , 2001
Cells
Osteoclasts
Enzymes
Medium
DMEM
Bone
Mouse
Mouse, 3-5 day, 6-8 week
Chen X. , Qian H. , Neff L. , Satomura K. and Horowitz M.
Thy-1 antigen Expression by Cells in the Osteoblast Lineage ,
J Bone Miner Res
 14 , 362 , 1999
Cells
Bone Cells, Osteoblasts
Enzymes
Collagenase Type 2 : 200-250 u/ml
Medium
DMEM
Bone
Mouse
Mouse, BALB/c
Takanashi, H. , Matsuishi, T. and Yoshizato, K.
Establishment and Characterization of Stromal Cell Lines That Support Differentiation of Murine Hematopoietic Blast Cells into Osteoblast-like Cells ,
In Vitro Cell Dev Biol
 30A , 384 , 1994
Cells
Osteoblast-like Cells
Stromal Cell Lines
Hematopoietic Blast Cells
Enzymes
Trypsin : 0.1%
Medium
Eagle's MEM
Bone
Mouse
Mouse, Swiss-Webster
Chen,T. and Feldman,D.
Regulation of 1,25-Dihydroxyvitamin D3 Receptors in Cultured Mouse Bone Cells ,
J Biol Chem
 256 , 5561 , 1981
Cells
Neonatal bone
Enzymes
Medium
Tris-buffered saline
Liver
Mouse

Mouse

Bahrami, E. , Geiger, T. , Steixner-Kumar, A. , Santacruz, D. , Viollet, C. and Dick, A.
An Optimized Protocol for Isolation of Hepatic Leukocytes Retrieved from Murine and NASH Liver Biopsies. ,
Star Protocols
 4 , 102597 , 2023
Cells
Leukocytes
Enzymes
Collagenase Type 4 : 100 u/ml
Medium
RPMI-1640
Liver
Mouse

7-10 week

Plata-Gómez, A. , Crespo, M. , Arregui, C. , Prado-Rivas, L. , Sabio, G. and Efeyan, A.
Protocol for the Assessment of mTOR Activity in Mouse Primary Hepatocytes. , STAR Protoc Vol. 2 , 100918 , 2021
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Mouse

Mouse

Jung, Y. , Zhao, M. and Svensson, K.
Isolation, Culture, and Functional Analysis of Hepatocytes from Mice with Fatty Liver Disease. ,
Star Protocols
 1 , 100222 , 2020
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Mouse
Mouse
Cabral, F. , Miller, C. , Kudrna, K. , Hass, B. , Daubendiek, J. , Kellar, B. and Harris, E.
Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion. ,
J Vis Exp
 , 2018
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Mouse
Mouse
Bigorgne, A. , John, B. , Ebrahimkhani, M. , Shimizu-Albergine, M. , Campbell, J. and Crispe, I.
TLR4-Dependent Secretion by Hepatic Stellate Cells of the Neutrophil-Chemoattractant CXCL1 Mediates Liver Response to Gut Microbiota. ,
PLoS ONE
 11 , e0151063 , 2016
Cells
Hepatic
Enzymes
Medium
HBSS
Liver
Mouse
Mouse, 10-13 week
Sewing, S. , Boess, F. , Moisan, A. , Bertinetti-Lapatki, C. , Minz, T. , Hedtjaern, M. , Tessier, Y. , Schuler, F. , Singer, T. and Roth, A.
Establishment of a Predictive In Vitro Assay for Assessment of the Hepatotoxic Potential of Oligonucleotide Drugs. ,
PLoS ONE
 11 , e0159431 , 2016
Cells
Hepatocytes
Enzymes
Collagenase Type 2 : 0.2 u/ml
Medium
William's Medium E
Liver
Mouse
Mouse
Weerasinghe, S. , Park, M. , Portney, D. and Omary, M.
Mouse Genetic Background Contributes to Hepatocyte Susceptibility to Fas-mediated Apoptosis. ,
Mol Biol Cell
 27 , 3005-3012 , 2016
Cells
Hepatocytes
Enzymes
Collagenase Type 2 : 150 u/ml
Medium
PBS
Liver
Mouse
Mouse, 12 week
Mederacke, I. , Dapito, D. , Affo, S. , Uchinami, H. and Schwabe, R.
High-Yield and High-Purity Isolation of Hepatic Stellate Cells from Normal and Fibrotic Mouse Livers. ,
Nat Protoc
 10 , 305-15 , 2015
Cells
Hepatic stellate
Enzymes
Pronase : 0.04%
Medium
DMEM
Liver
Mouse
Mouse
Guenthner, C. , Luitje, M. , Pyle, L. , Molyneux, P. , Yu, J. , Li, A. , Leise, T. and Harrington, M.
Circadian Rhythms of Per2::Luc in Individual Primary Mouse Hepatocytes and Cultures. ,
PLoS ONE
 9 , e87573 , 2014
Cells
Hepatocytes
Enzymes
Collagenase Type 3 : 100 u/ml
Collagenase Type 4 : 100 u/ml
Medium
DMEM
Liver
Mouse
Mouse
Sin, Y. , Ballantyne, L. , Mukherjee, K. , St Amand, T. , Kyriakopoulou, L. , Schulze, A. and Funk, C.
Inducible Arginase 1 Deficiency in Mice Leads to Hyperargininemia and Altered Amino Acid Metabolism. ,
PLoS ONE
 8 , e80001 , 2013
Cells
hepatocytes
Enzymes
Collagenase Type 4 : 100 u/ml
Medium
DMEM
Liver
Mouse
Mouse
Perepelyuk, M. , Terajima, M. , Wang, A. , Georges, P. , Janmey, P. , Yamauchi, M. and Wells, R.
Hepatic Stellate Cells and Portal Fibroblasts are the Major Cellular Sources of Collagens and Lysyl Oxidases in Normal Liver and Early After Injury. ,
Am J Physiol Gastrointest Liver Physiol
 304 , G605 , 2013
Cells
Hepatocytes and sinusoidal endothelial
Enzymes
Medium
HBSS
Liver
Mouse
Mouse, female, 8-10 week
Severgnini, M. , Sherman, J. , Sehgal, A. , Jayaprakash, N. , Aubin, J. , Wang, G. , Zhang, L. , Peng, C. , Yucius, K. , Butler, J. and Fitzgerald, K.
A Rapid Two-Step Method for Isolation of Functional Primary Mouse Hepatocytes: Cell Characterization and Asialoglycoprotein Receptor Based Assay Development. ,
Cytotechnology
 64 , 187-95 , 2012
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Mouse
Mouse
Brundert, M. , Heeren, J. , Merkel, M. , Carambia, A. , Herkel, J. , Groitl, P. , Dobner, T. , Ramakrishnan, R. , Moore, K. and Rinninger, F.
Scavenger Receptor CD36 Mediates Uptake of High Density Lipoproteins in Mice and by Cultured Cells. ,
J Lipid Res
 52 , 745 , 2011
Cells
Hepatocytes & non-parenchymal liver cells
Enzymes
Collagenase : 0.03-0.05%
Medium
DMEM
Liver
Mouse
Mouse
Kang, H. , Okamoto, K. , Kim, Y. , Takeda, Y. , Bortner, C. , Dang, H. , Wada, T. , Xie, W. , Yang, X. , Liao, G. and Jetten, A.
Nuclear Orphan Receptor TAK1/TR4-Deficient Mice are Protected Against Obesity-Linked Inflammation, Hepatic Steatosis, and Insulin Resistance. ,
Diabetes
 60 , 177 , 2011
Cells
Hepatocytes
Enzymes
HIS kit : with modifications
Medium
DMEM
Liver
Mouse
Mouse
Holl, D. , Kuckenberg, P. , Woynecki, T. , Egert, A. , Becker, A. , Huss, S. , Stabenow, D. , Zimmer, A. , Knolle, P. , Tolba, R. , Fischer, H. and Schorle, H.
Transgenic Overexpression of Tcfap2c/AP-2gamma Results in Liver Failure and Intestinal Dysplasia. ,
PLoS ONE
 6 , e22034 , 2011
Cells
Hepatacytes
Enzymes
Medium
Williams E
Liver
Mouse
Mouse
Rountree, C. , Ding, W. , Dang, H. , Vankirk, C. and Crooks, G.
Isolation of CD133+ Liver Stem Cells for Clonal Expansion. ,
J Vis Exp
 56 , e3183 , 2011
Cells
CD133+ stem
Enzymes
Pronase : 0.05%
Medium
DMEM/F12
Liver
Mouse
Mouse
Shmarakov, I. , Fleshman, M. , D'Ambrosio, D. , Piantedosi, R. , Riedl, K. , Schwartz, S. , Curley, R. , von Lintig, J. , Rubin, L. , Harrison, E. and Blaner, W.
Hepatic Stellate Cells are an Important Cellular Site for β-Carotene Conversion to Retinoid. ,
Arch Biochem Biophys
 504 , 3-10 , 2010
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Mouse
Mouse, male, 12-14 week
Chung, S. , Timmins, J. , Duong, M. , Degirolamo, C. , Rong, S. , Sawyer, J. , Singaraja, R. , Hayden, M. , Maeda, N. , Rudel, L. , Shelness, G. and Parks, J.
Targeted Deletion of Hepatocyte ABCA1 Leads to VLDL Triglyceride Over-Production and LDL Hypercatabolism ,
J Biol Chem
 285 , 12197 , 2010
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Mouse
Mouse
Bosschaerts, T. , Guilliams, M. , Stijlemans, B. , Morias, Y. , Engel, D. , Tacke, F. , Herin, M. , De Baetselier, P. and Beschin, A.
Tip-DC Development During Parasitic Infection is Regulated by IL-10 and Requires CCL2/CCR2, IFN-gamma and MyD88 Signaling. ,
PLoS Pathog
 6 , e1001045 , 2010
Cells
Liver non-parenchymal
Enzymes
Collagenase Type 3 : 100 u/ml
Medium
HBSS
Liver
Mouse
Mouse, male, adult
Mathijs, K. , Kienhuis, A. , Brauers, K. J, Jennen, D. , Lahoz, A. , Kleinjans, J. and van Delft, J.
Assessing the Metabolic Competence of Sandwich-Cultured Mouse Primary Hepatocytes. ,
Drug Metab Dispos
 37 , 1305 , 2009
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Mouse
Mouse, 7-10 week
Follenzi, A. , Benten, D. , Novikoff, P. , Faulkner, L. , Raut, S. and Gupta, S.
Transplanted Endothelial Cells Repopulate the Liver Endothelium and Correct the Phenotype of Hemophilia A Mice. ,
J Clin Invest
 118 , 935 , 2008
Cells
Liver endothelial
Enzymes
Collagenase : 0.03%
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.