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Tissue References Search Tool

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Bone
Mouse
Mouse
Mohrin, D. , Koukos, G. , Alabdulaaly, L. , Paw, J. , Hake, K. , Kolumam, G. , Bouxsein, M. , Baron, R. , Kutskova, Y. , Freund, A. , Liu, J. , Zavala-Solorio, J. , Bhargava, S. , Maxwell, Trumble, J. , Brito, A. , Hu, D. and Brooks, D.
Inhibition of Longevity Regulator PAPP-A Modulates Tissue Homeostasis via Restraint of Mesenchymal Stromal Cells. , Aging Cell Vol. 20 , e13313 , 2021
Cells
Bone and bone marrow stromal
Enzymes
Medium
PBS
Bone
Mouse
Mouse
Baryawno, N. , Przybylski, D. , Kowalczyk, M. , Kfoury, Y. , Severe, N. , Gustafsson, K. , Kokkaliaris, K. , Mercier, F. , Tabaka, M. , Hofree, M. , Dionne, D. , Papazian, A. , Lee, D. , Ashenberg, O. and Subramanian, A.
A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia. ,
Cell
 177 , 1915-1932.e16 , 2019
Cells
Bone and bone marrow stroma
Enzymes
Medium
Media 199
Bone
Mouse
Mouse
Wang, W. , Majihail, G. , Lui, C. and Zhou, L.
Osteoblast Sorting and Intracellular Staining of CXCL12. ,
Bio Protoc
 8 , 2018
Cells
Osteoblast
Enzymes
Medium
HBSS
Bone
Mouse
Mouse
Agarwal, S. , Loder, S. , Sorkin, M. , Li, S. , Shrestha, S. , Zhao, B. , Mishina, Y. , James, A. and Levi, B.
Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification. ,
Stem Cells
 34 , 1692-701 , 2016
Cells
Bone-cartilage-stromal
Enzymes
Medium
HBSS
Bone
Mouse
Mouse
Igarashi, H. , Akahoshi, N. , Ohto-Nakanishi, T. , Yasuda, D. and Ishii, S.
The Lysophosphatidic Acid Receptor LPA4 Regulates Hematopoiesis-Supporting Activity of Bone Marrow Stromal Cells. ,
Sci Rep
 5 , 11410 , 2015
Cells
Bone stromal
Enzymes
Medium
DMEM
Bone
Mouse
Mouse
Kode, A.,Manavalan1, J.,Mosialou1, I. , Bhagat G. and Rathinam, C, Luo, N et al.
Leukemogenesis Induced by an Activating β-catenin Mutation in Osteoblasts ,
Nature
 506 , 240 , 2014
Cells
Osteoblasts
Enzymes
Medium
MEM
Bone
Mouse
Mouse
Lee, D. , Choung, H. , Kim, H. , Gronostajski, R. , Yang, Y. , Ryoo, H. , Lee, Z. , Kim, H. , Cho, E. and Park, J.
NFI-C Regulates Osteoblast Differentiation Via Control of Osterix Expression. ,
Stem Cells
 32 , 2467-79 , 2014
Cells
Osteoblasts
Enzymes
Medium
MEM
Bone
Mouse
Mouse, 2-5 day
Taylor, S. , Shah, M. and Orriss, I.
Generation of Rodent and Human Osteoblasts. ,
Bonekey Rep
 3 , 585 , 2014
Cells
Osteoblasts
Enzymes
Trypsin : 0.25%-1.0%
Medium
DMEM
Bone
Mouse
Mouse, 4-22 month
Stern, A. , Stern, M. , Van Dyke, M. , Jahn, K. , Prideaux, M. and Bonewald, L.
Isolation and Culture of Primary Osteocytes from the Long Bones of Skeletally Mature and Aged Mice. ,
Biotechniques
 52 , 361 , 2012
Cells
Osteocytes
Enzymes
Collagenase Type 1 : 300 u/ml
Medium
MEM
Bone
Mouse
Mouse
Nakamura, Y. , Arai, F. , Iwasaki, H. , Hosokawa, K. , Kobayashi, I. , Gomei, Y. , Matsumoto, Y. , Yoshihara, H. and Suda, T.
Isolation and Characterization of Endosteal Niche Cell Populations that Regulate Hematopoietic Stem Cells. ,
Blood
 116 , 1422 , 2010
Cells
Endosteal cells
Enzymes
Medium
DMEM
Bone
Mouse
Mouse
Morikawa, S. , Mabuchi, Y. , Kubota, Y. , Nagai, Y. , Niibe, K. , Hiratsu, E. , Suzuki, S. , Miyauchi-Hara, C. , Nagoshi, N. , Sunabori, T. , Shimmura, S. , Miyawaki, A. , Nakagawa, T. , Suda, T. , Okano, H. and Matsuzaki, Y.
Prospective Identification, Isolation, and Systemic Transplantation of Multipotent Mesenchymal Stem Cells in Murine Bone Marrow. ,
J Exp Med
 206 , 2483-96 , 2009
Cells
Bone marrow
Enzymes
Collagenase : 0.2%
Medium
DMEM
Bone
Mouse
Mouse, male, 6-8 week old
Sakai, E. , Miyamoto, H. , Okamoto, K. , Kato, Y. , Yamamoto, K. and Sakai, H.
Characterization of Phagosomal Subpopulations Along Endocytic Routes in Osteoclasts and Macrophages ,
J. Biochem.
 130 , 823 , 2001
Cells
Osteoclasts
Enzymes
Medium
DMEM
Bone
Mouse
Mouse, 3-5 day, 6-8 week
Chen X. , Qian H. , Neff L. , Satomura K. and Horowitz M.
Thy-1 antigen Expression by Cells in the Osteoblast Lineage ,
J Bone Miner Res
 14 , 362 , 1999
Cells
Bone Cells, Osteoblasts
Enzymes
Collagenase Type 2 : 200-250 u/ml
Medium
DMEM
Bone
Mouse
Mouse, BALB/c
Takanashi, H. , Matsuishi, T. and Yoshizato, K.
Establishment and Characterization of Stromal Cell Lines That Support Differentiation of Murine Hematopoietic Blast Cells into Osteoblast-like Cells ,
In Vitro Cell Dev Biol
 30A , 384 , 1994
Cells
Osteoblast-like Cells
Stromal Cell Lines
Hematopoietic Blast Cells
Enzymes
Trypsin : 0.1%
Medium
Eagle's MEM
Bone
Mouse
Mouse, Swiss-Webster
Chen,T. and Feldman,D.
Regulation of 1,25-Dihydroxyvitamin D3 Receptors in Cultured Mouse Bone Cells ,
J Biol Chem
 256 , 5561 , 1981
Cells
Neonatal bone
Enzymes
Medium
Tris-buffered saline
Spleen
Mouse

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.
Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
 13 , e4679 , 2023
Cells
T cells
Enzymes
Medium
RPMI
Spleen
Mouse
Mouse, 4 week
Hou, M. , Han, J. , Li, G. , Kwon, M. , Jiang, J. , Emani, S. , Taglauer, E. , Park, J. , Choi, E. , Vodnala, M. , Fong, Y. , Emani, S. , Rosas, I. , Perrella, M. and Liu, X.
Multipotency of Mouse Trophoblast Stem Cells ,
Stem Cell Res Ther
 11 , 55 , 2020
Cells
Trophoblast
Enzymes
Medium
DMEM
Spleen
Mouse
Mouse
Villarreal, E. , Hewgley, W. , Lang, W. , Morton, C. , Mao, S. , Wu, J. and Sandoval, J.
In-bag Enzymatic Splenic Digestion: A Novel Alternative to Manual Morcellation? ,
J Surg Res
 218 , 209-216 , 2017
Cells
Speen
Enzymes
Medium
HBSS
Spleen
Mouse
Mouse
Klebanoff, C. , Spencer, S. , Torabi-Parizi, P. , Grainger, J. , Roychoudhuri, R. , Ji, Y. , Sukumar, M. , Muranski, P. , Scott, C. , Hall, J. , Ferreyra, G. , Leonardi, A. and Borman, Z. et al.
Retinoic Acid Controls the Homeostasis of Pre-cDC-Derived Splenic and Intestinal Dendritic Cells. ,
J Exp Med
 210 , 1961 , 2013
Cells
Splenic
Enzymes
Medium
PBS
Spleen
Mouse
Mouse
Yi, T. and Cyster, J.
EBI2-Mediated Bridging Channel Positioning Supports Splenic Dendritic Cell Homeostasis and Particulate Antigen Capture. ,
Elife
 2 , e00757 , 2013
Cells
Splenocytes
Enzymes
Medium
PBS
Spleen
Mouse
Mouse
Shi, C. , Jia, T. , Mendez-Ferrer, S. , Hohl, T. , Serbina, N. , Lipuma, L. , Leiner, I. , Li, M. , Frenette, P. and Pamer, E.
Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands. ,
Immunity
 34 , 590 , 2011
Cells
Spleen, bone marrow endothelial
Enzymes
Medium
PBS
Spleen
Mouse
Mouse
Flano, E. , Jewell, N. , Durbin, R. and Durbin, J.
Methods Used to Study Respiratory Virus Infection. , Curr Protoc Cell Biol Vol. Chapter 26 , Unit 26.3 , 2009
Cells
Dendritic
Enzymes
Medium
HBSS
Spleen
Mouse
Mouse, female, 6-8 week
Abou Fakher, F. , Rachinel, N. , Klimczak, M. , Louis, J. and Doyen, N.
TLR9-Dependent Activation of Dendritic Cells by DNA from Leishmania Major Favors Th1 Cell Development and the Resolution of Lesions. ,
J Immunol
 182 , 1386 , 2009
Cells
Dendritic
Enzymes
Medium
RPMI 1640
Spleen
Mouse
Mouse
Abe, K. , Nguyen, K. , Fine, S. , Mo, J. , Shen, C. , Shenouda, S. , Corr, M. , Jung, S. , Lee, J. , Eckmann, L. and Raz, E.
Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells. ,
Proc Natl Acad Sci U S A
 104 , 17022 , 2007
Cells
Dentritic
Enzymes
Collagenase : 300 u/ml
Medium
RPMI 1640
Spleen
Mouse
Mouse
Kang, H. , Liu, M. and Datta, S.
Low-dose Peptide Tolerance Therapy of Lupus Generates Plasmacytoid Dendritic Cells That Cause Expansion of Autoantigen-Specific Regulatory T Cells and Contraction of Inflammatory Th17 Cells ,
J Immunol
 178 , 7849-58 , 2007
Cells
Splenic dendritic
Enzymes
Medium
DMEM
Spleen
Mouse
Mouse
Mueller, S. , Matloubian, M. , Clemens, D, Sharpe, A. , Freeman, G. , Gangappa, S. , Larsen, C. and Ahmed, R.
Viral Targeting of Fibroblastic Reticular Cells Contributes to Immunosuppression and Persistence During Chronic Infection. ,
Proc Natl Acad Sci U S A
 104 , 15430 , 2007
Cells
Splenic
Enzymes
Medium
RPMI
Spleen
Mouse
Mouse
Benedict Chris A, De Trez Carl, Schneider Kirsten, Ha Sukwon, Patterson Ginelle, Ware Carl F.
Specific remodeling of splenic architecture by cytomegalovirus ,
PLoS Pathog
 2 , e16 , 2006
Cells
Splenic stromal
Enzymes
Collagenase Type 3 : 100-400 u/ml
Medium
HBSS
Spleen
Mouse
Mouse
Siragam, V. , Crow, A. , Brinc, D. , Song, S. , Freedman, J. and Lazarus, A.
Intravenous Immunoglobulin Ameliorates ITP via Activating Fc Gamma Receptors on Dendritic Cells. ,
Nat Med
 12 , 688 , 2006
Cells
Leukocytes
Enzymes
Collagenase Type 4 : 43 u/ml
Medium
RPMI 1640
Spleen
Mouse
Mouse
Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F. and Gabrieli, L
ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells ,
J Exp Med
 196 , 1415 , 2002
Cells
Dentritic
Enzymes
Medium
RPMI 1640
Spleen
Mouse
Mouse, 4-6 week
McLellan, A. , Kapp, M. , Eggert, A. , Linden, C. , Bommhardt, U. , Brocker, E. , Kammerer, U. and Kampgen, E.
Anatomic Location and T-cell Stimulatory Functions of Mouse Dendritic Cell Subsets Defined by CD4 and CD8 Expression ,
Blood
 99 , 2084-93 , 2002
Cells
Dentritic
Enzymes
Medium
RPMI-1640
Spleen
Mouse
Mouse
Stagg, AJ, Burke, F, Hill, S. and Knight, S.
Isolation of Mouse Spleen Dendritic Cells. ,
Methods Mol Med
 64 , 9-22 , 2001
Cells
Dentritic cells
Enzymes
Medium
HEPES/RPMI 1640
Spleen
Mouse
Mouse
Brasel K, De Smedt T, Smith JL, Maliszewski CR.
Generation of murine dendritic cells from flt3-ligand-supplemented bone marrow cultures ,
Blood
 96 , 3029-39 , 2000
Cells
Dentritic
Enzymes
Collagenase : 100 u/ml
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.