Tissue References Search Tool

Mouse

Mouse, 2-5 day

Otsuru, S. , Hofmann, T. , Raman, P. , Olson, T. , Guess, A. , Dominici, M. and Horwitz, E.

Genomic and Functional Comparison of Mesenchymal Stromal Cells Prepared Using Two Isolation Methods. ,
Cytotherapy
17 , 262-70 , 2015

Cells

Chondrocytes

Enzymes

Trypsin : 0.25%

Collagenase Type 1 : 86.5 u/ml

Medium

DMEM

Cartilage

Mouse

Mouse, 1 day

Terpstra, L, Prud'homme, J, Arabian, A, Takeda, S, Karsenty, G, Dedhar, S. and St-Arnaud, R.

Reduced Chondrocyte Proliferation and Chondrodysplasia in Mice Lacking the Integrin-linked Kinase in Chondrocytes ,
J Cell Biol
162 , 139 , 2003

Cells

Chondrocytes

Enzymes

Collagenase : 0.2%

Medium

DMEM

Organoids

Mouse

Fujimura, T. , Enomoto, Y. , Katsura, H. , Ogawa, T. , Baba, S. , Ogata, A. , Yamaoka, A. , Shiroguchi, K. and Morimoto, M.

Identifying a Lung Stem Cell Subpopulation by Combining Single-Cell Morphometrics, Organoid Culture, and Transcriptomics. ,
Stem Cells
41 , 809-820 , 2023

Cells

Lung

Enzymes

Collagenase Type 1 : 450 u/ml

Elastase : 1 u/ml

Neutral Protease : 5 u/ml

Deoxyribonuclease I : 0.005%

Medium

DMEM/F12

Organoids

Mouse

Klemke, L. , Blume, J. , Oliveira, T. and Schulz-Heddergott, R.

Preparation and Cultivation of Colonic and Small Intestinal Murine Organoids Including Analysis of Gene Expression and Organoid Viability. ,
Bio Protoc
12 , e4298 , 2022

Cells

Colonic tumor organoids

Enzymes

Collagenase Type 1 : 0.2%

Medium

PBS

Organoids

Mouse

Huang, L. , Desai, R. , Conrad, D. , Leite, N. , Akshinthala, D. , Lim, C. , Gonzalez, R. , Muthuswamy, L. , Gartner, Z. and Muthuswamy, S.

Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids. , Cell Stem Cell Vol. 28 , 1090-1104.e6 , 2021

Cells

Organoid cultures

Enzymes

Stemxyme 1 : 0.1%

Medium

PBS

Organoids

Mouse

Charifou, E. , Sumbal, J. , Koledova, Z. , Li, H. and Chiche, A.

A Robust Mammary Organoid System to Model Lactation and Involution-like Processes. ,
Bio Protoc
11 , e3996 , 2021

Cells

Mammary organoids

Enzymes

Collagenase Type 1 : 0.2%

Trypsin : 0.2%

Medium

DMEM/F12

Organoids

Mouse

Mouse, adult

Neal, J,, Li ,X. , Zhu, J. , Giangarra, V. , Grzeskowiak, C. , Ju, J. , Liu, I. Chiou, S. , Salahudeen, A. and Smith, A. et al

Organoid Modeling of the Tumor Immune Microenvironment. ,
Cell
175 , 1972 , 2018

Cells

Organoids

Enzymes

Collagenase Type 4 : 200-300 u/ml

Medium

DMEM

Organoids

Mouse

Mouse, embryonic

Nikolić, M. , Caritg, O. , Jeng, Q. , Johnson, J. , Sun, D. , Howell, K. , Brady, J. , Laresgoiti, U. , Allen, G. , Butler, R. , Zilbauer, M. , Giangreco, A. and Rawlins, E.

Human Embryonic Lung Epithelial Tips are Multipotent Progenitors that can be Expanded In vitro as Long-term Self-renewing Organoids. ,
Elife
6 , 2017

Cells

Lung organoids

Enzymes

Neutral Protease : 24 u/ml

Deoxyribonuclease I : 0.001%

Medium

DMEM/F12

Organoids

Mouse

O'Rourke, K. , Ackerman, S. , Dow, L. and Lowe, S.

Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells. ,
Bio Protoc
6 , 2016

Cells

Intestinal organoids

Enzymes

Collagenase Type 4 : 500 u/ml

Deoxyribonuclease I : 15 u/ml

Medium

Basal Medium

Organoids

Mouse

Mouse, 8-12 week

Huch, M. , Bonfanti, P. , Boj, S. , Sato, T. , Loomans, C. , Van de Wetering, M. , Sojoodi, M. , Li, V. , Schuijers, J. , Gracanin, A. , Ringnalda, F. , Begthel, H. , Hamer, K. , Mulder, J. , Van Es, J. and De Koning, E.

Unlimited In Vitro Expansion of Adult Bi-Potent Pancreas Progenitors Through the Lgr5/R-Spondin Axis. ,
EMBO J
32 , 2708-21 , 2013

Cells

Pancreas organoid

Enzymes

Neutral Protease : 0.012%

Collagenase : 0.012%

Medium

DMEM

Organoids

Mouse

Xue, X. and Shah, Y.

In Vitro Organoid Culture of Primary Mouse Colon Tumors. ,
J Vis Exp
, e50210 , 2013