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Tissue References Search Tool

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Eye
Mouse

Mouse, 2 month

Murenu, E. , Pavlou, M. , Richter, L. , Rapti, K. , Just, S. and Cehajic-Kapetanovic, J.
A Universal Protocol for Isolating Retinal ON Bipolar Cells Across Species via Fluorescence-Activated Cell Sorting. , Mol Ther Methods Clin Dev Vol. 20 , 587-600 , 2021
Enzymes
Papain : 20 u/ml
Medium
EBSS
Eye
Mouse
Mouse, 2-4 week
Liu, X. , Tang, L. and Liu Y.
Mouse Muller Cell Isolation and Culture. ,
Bio-protocol
 7 , 2017
Cells
Muller
Enzymes
PDS kit : per instructions
Medium
DMEM
Eye
Mouse
Mouse
Ortin-Martinez, A. , Tsai, E. , Nickerson, P. , Bergeret, M. , Lu, Y. , Smiley, S. , Comanita, L. and Wallace, V.
A Reinterpretation of Cell Transplantation: GFP Transfer From Donor to Host Photoreceptors. ,
Stem Cells
 35 , 932-939 , 2017
Cells
Retinal
Enzymes
Papain : 20 u/ml
Medium
PBS
Eye
Mouse
Mouse
Wohl, S. , Jorstad, N. , Levine, E. and Reh, T.
Muller Glial microRNAs are Required for the Maintenance of Glial Homeostasis and Retinal Architecture. ,
Nat Commun
 8 , 1603 , 2017
Cells
Retinal
Enzymes
Papain : 20 u/ml
Medium
HBSS
Eye
Mouse
Mouse, C57BL/6
Blandford, S. and Baldridge, W.
The Effect of Glutamate Receptor Agonists on Mouse Retinal Astrocyte [Ca(2+)]i. ,
Biomed Res Int
 2016 , 8178162 , 2016
Cells
Retinal astrocytes
Enzymes
Papain : 16.5 u/ml
Medium
HBSS
Eye
Mouse
Mouse, adult
Feodorova, Y. , Koch, M. , Bultman, S. , Michalakis, S. and Solovei, I.
Quick and Reliable Method for Retina Dissociation and Separation of Rod Photoreceptor Perikarya from Adult Mice. ,
MethodsX
 2 , 39 , 2015
Cells
Retinal photoreceptor
Enzymes
PDS kit : with modifications
Medium
EBSS
Eye
Mouse
Mouse, 4-8 day
Balmer, J. , Zulliger, R. , Roberti, S. and Enzmann, V.
Retinal Cell Death Caused by Sodium Iodate Involves Multiple Caspase-Dependent and Caspase-Independent Cell-Death Pathways. ,
Int J Mol Sci
 16 , 15086-103 , 2015
Cells
Photoreceptors
Enzymes
PDS kit : per instructions
Medium
Neurobasal
Eye
Mouse
Mouse, embryonic and P0
Dvoriantchikova, G. , Perea-Martinez, I. , Pappas, S. , Barry, A. , Danek, D. , Dvoriantchikova, X. , Pelaez, D. and Ivanov, D.
Molecular Characterization of Notch1 Positive Progenitor Cells in the Developing Retina. ,
PLoS ONE
 10 , e0131054 , 2015
Cells
Retinal
Enzymes
Papain : 8 u/ml
Deoxyribonuclease I : 124 u/ml
Medium
DPBS
Eye
Mouse
Mouse
Iqbal, N. , Xu, L. , Devitt, C. and Skapek, S.
Isolation and Characterization of Mammalian Cells Expressing the Arf Promoter During Eye Development. ,
Biotechniques
 56 , 239-49 , 2014
Cells
Vitreous cells
Enzymes
Collagenase : 0.1%
Hyaluronidase : 0.03%
Medium
DMEM
Eye
Mouse
Mouse, 6 week
Singh, M. , Issa, P, Butler, R. , Martin, C. , Lipinski, D. , Sekaran, S. , Barnard, A. and Maclaren, R.
Reversal of End-Stage Retinal Degeneration and Restoration of Visual Bunction by Photoreceptor Transplantation. ,
Proc Natl Acad Sci U S A
 , 2013
Cells
Retinal
Enzymes
PDS kit : per instructions
Medium
DMEM
Eye
Mouse
Mouse, adult
Gualdoni, S. , Baron, M. , Lakowski, J. , Decembrini, S. , Pearson, R. , Ali, R. and Sowden, J.
Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells. ,
Curr Protoc Stem Cell Biol
 Chapter 1 , Unit 1H.4 , 2011
Cells
Cilary epithelial
Enzymes
PDS kit : per instructions
Medium
EBSS
Eye
Mouse
Mouse
Schmidt, T. and Kofuji, P.
An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells. ,
J Vis Exp
 47 , 2367 , 2011
Cells
Retinal ganglion
Enzymes
CLSPA : 240 u/ml
Hyaluronidase : 0.2%
Medium
Ames
Eye
Mouse
Mouse, 4-5 week
Ebihara, L. , Tong, J. , Vertel, B. , White, T. and Chen, T.
Properties of Connexin 46 Hemichannels in Dissociated Lens Fiber Cells. ,
Inv Ophthal Visual Sci
 52 , 882-9 , 2011
Cells
Lens Fiber
Enzymes
Protease : 0.5%
Medium
PBS
Eye
Mouse
Mouse, postnatal day 1
Jiang, C. Klassen, H. , Zhang, X. and Young, M.
Laser Injury Promotes Migration and Integration of Retinal Progenitor Cells into Host Retina. ,
Mol Vis
 16 , 983 , 2010
Cells
Retinal progenitor
Enzymes
Collagenase : 0.1%
Medium
HBSS
Eye
Mouse
Mouse, 4-8 week
Kobayashi, T. , Yoshioka, R. , Shiraishi, A. and Ohashi, Y.
New Technique for Culturing Corneal Epithelial Cells of Normal Mice. ,
Mol Vis
 15 , 1589-93 , 2009
Cells
Corneal epithelial
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Eye
Mouse
Mouse
Amadi-Obi, A. , Yu, C. , Liu, X. , Mahdi, R. , Clarke, G. , Nussenblatt, R, Gery, I. , Lee, Y. and Egwuagu, C.
TH17 Cells Contribute to Uveitis and Scleritis and are Expanded by IL-2 and Inhibited by IL-27/STAT1. ,
Nat Med
 13 , 711 , 2007
Cells
Retinal
Enzymes
Medium
RPMI
Eye
Mouse
mouse, adult
Jadhav AP, Cho SH. and Cepko CL
Notch activity permits retinal cells to progress through multiple progenitor states and acquire a stem cell property ,
Proc Natl Acad Sci U S A
 103 , 18998 , 2006
Cells
Retinal
Enzymes
Papain : 50 u/ml
Medium
DMEM/F-12
Eye
Mouse
Mouse, adult
Yoshida, S. , Shimmura, S. , Nagoshi, N. , Fukuda, K. , Matsuzaki, Y. , Okano, H. and Tsubota, K.
Isolation of Multipotent Neural Crest-Derived Stem Cells from the Adult Mouse Cornea. ,
Stem Cells
 24 , 2714-22 , 2006
Cells
Stromal
Enzymes
Trypsin : 0.05%
Collagenase : 78 u/ml
Hyaluronidase : 38 u/ml
Medium
DMEM/F-12
Eye
Mouse
Mouse, 2-5 month
Maxeiner Stephan, Dedek Karin, Janssen-Bienhold Ulrike, Ammermuller Josef, Brune Hendrik, Kirsch Taryn, Pieper Mario, Degen Joachim, Kruger Olaf, Willecke Klaus, Weiler Reto.
Deletion of connexin45 in mouse retinal neurons disrupts the rod/cone signaling pathway between AII amacrine and ON cone bipolar cells and leads to impaired visual transmission ,
J Neurosci
 25 , 566-76 , 2005
Cells
Mouse retinal and bipolar
Enzymes
Papain : 20 u/ml
Deoxyribonuclease I : 200 u/ml
Medium
HBSS
Eye
Mouse
Mouse, 4 week
Su X, Sorenson CM. and Sheibani N.
Isolation and characterization of murine retinal endothelial cells ,
Mol Vis
 9 , 171 , 2003
Cells
Retinal endothelial cells
Enzymes
Medium
DMEM
Pancreas
Mouse
Mouse
Low, L. , Zavortink, M. , Mitchell, J. , Gan, W. , Do, O. , Schwiening, C. , Gaisano, H. and Thorn, P.
Insulin Secretion from Beta Cells in Intact Mouse Islets is Targeted Towards the Vasculature ,
Diabetologia
 57 , 1655 , 2014
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Mouse
Mouse
Koh, D. , Moody, M. and Jo, J.
Collection of Islets of Langerhans using an Equilibrium Method. ,
Biotechniques
 55 , 34-7 , 2013
Cells
Islets
Enzymes
Collagenase : 0.1-0.25%
Medium
HBSS
Pancreas
Mouse
Mouse, 8-12 week
Huch, M. , Bonfanti, P. , Boj, S. , Sato, T. , Loomans, C. , Van de Wetering, M. , Sojoodi, M. , Li, V. , Schuijers, J. , Gracanin, A. , Ringnalda, F. , Begthel, H. , Hamer, K. , Mulder, J. , Van Es, J. and De Koning, E.
Unlimited In Vitro Expansion of Adult Bi-Potent Pancreas Progenitors Through the Lgr5/R-Spondin Axis. ,
EMBO J
 32 , 2708-21 , 2013
Cells
Pancreas organoid
Enzymes
Neutral Protease : 0.012%
Collagenase : 0.012%
Medium
DMEM
Pancreas
Mouse
Mouse, embryonic
Greggio, C. , De Franceschi, F. , Figueiredo-Larsen, M. , Gobaa, S. , Ranga, A. , Semb, H. , Lutolf, M. and Grapin-Botton, A.
Artificial Three-Dimensional Niches Deconstruct Pancreas Development In Vitro. ,
Development
 140 , 4452-62 , 2013
Cells
Pancreatic progenitor
Enzymes
Neutral Protease : 0.125%
Medium
DMEM
Pancreas
Mouse
Mouse
Bertera, S. , Balamurugan, A. , Bottino, R. , He, J. and Trucco, M.
Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum Albumin. ,
J Transplant
 2012 , 856386 , 2012
Cells
Islets
Enzymes
Collagenase : 0.2%
Medium
RPMI 1640
Pancreas
Mouse
Mouse
Ding, G. , Wang, F. , Shu, J. , Tian, S. , Jiang, Y. , Zhang, D. , Wang, N. , Luo, Q. , Zhang, Y. , Jin, F. , Leung, P. , Sheng, J. and Huang, H.
Transgenerational Glucose Intolerance with Igf2/H19 Epigenetic Alterations in Mouse Islet Induced by Intrauterine Hyperglycemia. ,
Diabetes
 61 , 1133-42 , 2012
Cells
Islet
Enzymes
Medium
RPMI-1640
Pancreas
Mouse
Mouse, 10 week
Kobayashi, T. , Yamaguchi, T. , Hamanaka, S. , Kato-Itoh, M. , Yamazaki, Y. , Ibata, M. , Sato, H. , Lee, Y. , Usui, J. , Knisely, A. , Hirabayashi, M. and Nakauchi, H.
Generation of Rat Pancreas in Mouse by Interspecific Blastocyst Injection of Pluripotent Stem Cells. ,
Cell
 142 , 787 , 2010
Cells
Islets
Enzymes
Collagenase : 0.2%
Medium
RPMI 1640
Pancreas
Mouse
Mouse, 6-8 month
Taguchi, Y. , Tasaki, Y. , Terakado, K. , Kobayashi, K. , Machida, T. and Kobayashi, T.
Impaired Insulin Secretion from the Pancreatic Islets of Hypothyroidal Growth-Retarded Mice. ,
J Endocrinol
 206 , 195-204 , 2010
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Mouse
Mouse
Li, D. , Yuan, Y. , Tu, H. , Liang, Q. and Dai, L.
A Protocol for Islet Isolation from Mouse Pancreas. ,
Nat Protoc
 4 , 1649 , 2009
Cells
Islets
Enzymes
Collagenase : 1,000 u/ml
Medium
RPMI 1640
Pancreas
Mouse
Mouse
Carter, J. , Dula, S. , Corbin, K. , Wu, R. and Nunemaker, C.
A Practical Guide to Rodent Islet Isolation and Assessment. ,
Biol Proced Online
 11 , 3 , 2009
Cells
Islets
Enzymes
Collagenase : 0.14%
Medium
RPMI 1640
Pancreas
Mouse
Mouse, adult, male
Ji, B. , Gaiser, S. , Chen, X. , Ernst, S. and Logsdon, C.
Intracellular Trypsin Induces Pancreatic Acinar Cell Death but not NF-KappaB Activation. ,
J Biol Chem
 284 , 17488 , 2009
Cells
Ancinar
Enzymes
CLSPA : see reference
Medium
DMEM
Pancreas
Mouse
Mouse, 3-4 week, 20-24 g
Huang, H. , Xie, Q. , Kang, M. , Zhang, B. , Zhang, H. , Chen, J. , Zhai, C. , Yang, D. , Jiang, B. and Wu, Y.
Labeling Transplanted Mice Islet with Polyvinylpyrrolidone Coated Superparamagnetic Iron Oxide Nanoparticles for In Vivo Detection By Magnetic Resonance Imaging. ,
Nanotechnology
 20 , 365101 , 2009
Cells
Islets
Enzymes
Medium
RPMI 1540
Pancreas
Mouse
Mouse
Szot, G. , Koudria, P. and Bluestone, J.
Murine Pancreatic Islet Isolation. ,
J Vis Exp
 7 , 255 , 2007
Cells
Islets
Enzymes
Collagenase : 0.03-0.08%
Medium
RPMI 1640
Pancreas
Mouse
Mouse, 6-10 month
Wang, Y. , Soyombo, A. , Shcheynikov, N. , Zeng, W. , Dorwart, M. , Marino, C. , Thomas, P. and Muallem, S.
Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis. ,
EMBO J
 25 , 5049 , 2006
Cells
Pancreatic ductal
Enzymes
CLSPA : 50 u/ml
Hyaluronidase : 400 u/ml
Medium
DMEM
Pancreas
Mouse
Mouse, adult
Voronina, S. , Barrow, S. , Gerasimenko, O. , Petersen, O. and Tepikin, A.
Effects of Secretagogues and Bile Acids on Mitochondrial Membrane Potential of Pancreatic Acinar Cells: Comparison of Different Modes of Evaluating Delta Psi. ,
J Biol Chem
 279 , 27327 , 2004
Cells
Acinar
Enzymes
CLSPA : 200 u/ml
Medium
see reference
Pancreas
Mouse
Mouse, 11-12 week
Astrof, S. , Crowley, D. , George, E. , Fukuda, T. , Sekiguchi, K. , Hanahan, D. and Hynes, R.
Direct Test of Potential Roles of EIIIA and EIIIB Alternatively Spliced Segments of Fibronectin in Physiological and Tumor Angiogenesis. ,
Mol Cell Biol
 24 , 8662-70 , 2004
Cells
Islets
Enzymes
Medium
RPMI

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.