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Tissue References Search Tool

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Colon
Mouse
Mouse
Larmonier, C. , Shehab, K. , Laubitz, D. , Jamwal, D. , Ghishan, F. and Kiela, P.
Transcriptional Reprogramming and Resistance to Colonic Mucosal Injury in Poly(ADP-ribose) Polymerase 1 (PARP1)-deficient Mice. ,
J Biol Chem
 291 , 8918-30 , 2016
Cells
Lamina propria
Enzymes
Collagenase : 100 u/ml
Medium
RPMI 1640
Colon
Mouse
Mouse
Fattahi, F. , Steinbeck, J. , Kriks, S. , Tchieu, J. , Zimmer, B. , Kishinevsky, S. , Zeltner, N. , Mica, Y. , El-Nachef, W. , Zhao, H. , de Stanchina, E. , Gershon, M. , Grikscheit, T. , Chen, S. and Studer, L.
Deriving Human ENS Lineages for Cell Therapy and Drug Discovery in Hirschsprung Disease. ,
Nature
 531 , 105-9 , 2016
Cells
Colon organoid
Enzymes
Collagenase Type 1 : 800 u/ml
Neutral Protease : 0.013%
Medium
DMEM
Colon
Mouse
Mouse, 8-10 week
Round, J. and Mazmanian, S.
Inducible Foxp3+ Regulatory T-cell Development by a Commensal Bacterium of the Intestinal Microbiota. ,
Proc Natl Acad Sci U S A
 107 , 12204-9 , 2010
Cells
Lamina propria lymphocytes
Enzymes
Medium
HBSS
Colon
Mouse
Mouse
Atarashi, K. , Nishimura, J. , Shima, T. , Umesaki, Y. , Yamamoto, M. , Onoue, M. , Yagita, H. , Ishii, N. , Evans, R. , Honda, K. and Takeda, K.
ATP Drives Lamina Propria T(H)17 Cell Differentiation. ,
Nature
 455 , 808 , 2008
Cells
Lamina propria
Enzymes
Medium
RPMI 1640
Colon
Mouse
Mouse
Weigmann Benno, Tubbe Ingrid, Seidel Daniel, Nicolaev Alex, Becker Christoph, Neurath Markus F.
Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue ,
Nat Protoc
 2 , 2307-11 , 2007
Cells
Lamina propria mononuclear cells
Enzymes
Medium
HBSS
Colon
Mouse
Mouse
Abe, K. , Nguyen, K. , Fine, S. , Mo, J. , Shen, C. , Shenouda, S. , Corr, M. , Jung, S. , Lee, J. , Eckmann, L. and Raz, E.
Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells. ,
Proc Natl Acad Sci U S A
 104 , 17022 , 2007
Cells
Dentritic
Enzymes
Collagenase : 300 u/ml
Medium
RPMI 1640
Colon
Mouse
Mouse, 6-8 week
Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F.
Phenotypic characterization of human colorectal cancer stem cells ,
Proc Natl Acad Sci U S A
 104 , 10158-63 , 2007
Cells
Cancer stem cell
Enzymes
Collagenase Type 3 : 200 u/ml
Deoxyribonuclease I : 100 u/ml
Medium
RPMI-1640
Colon
Mouse
Mouse
Annacker O, Coombes JL, Malmstrom V, Uhlig HH, Bourne T, Johansson-Lindbom B, Agace WW, Parker CM, Powrie F.
Essential role for CD103 in the T cell-mediated regulation of experimental colitis ,
J Exp Med
 202 , 1051 , 2005
Cells
Lympocytes
Enzymes
Collagenase/Dispase : 100 u/ml
Medium
RPMI 1640
Colon
Mouse
Mouse, 6-8 week
Totsuka T, Kanai T, Uraushihara K, Iiyama R, Yamazaki M, Akiba H, Yagita H, Okumura K, Watanabe M.
Therapeutic effect of anti-OX40L and anti-TNF-alpha MAbs in a murine model of chronic colitis ,
Am J Physiol/Gastro
 284 , G595-603 , 2003
Cells
Lamina propria lymphocytes
Enzymes
Medium
HBSS
Colon
Mouse
Mouse, 6-8 week
Wirtz S. , Becker C. , Blumberg R. , Galle P. and Neurath M.
Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA ,
J Immunol
 168(1) , 411 , 2002
Cells
Lamina propria mononuclear cells
Enzymes
Medium
RPMI
Mammary
Mouse

Mouse, female

Sun, H. , Xu, X. and Deng, C.
Preparation of Single Epithelial Cells Suspension from Mouse Mammary Glands. ,
Bio Protoc
 10 , e3530 , 2020
Cells
Mammary epithelial
Enzymes
Medium
DMEM/F-12
Mammary
Mouse
Mouse
Liu, X. , Johnson, S. , Liu, S. , Kanojia, D. , Yue, W. , Singh, U. , Wang, Q, Wang Qi, Nie, Q. and Chen H.
Nonlinear Growth Kinetics of Breast Cancer Stem Cells: Implications for Cancer Stem Cell Targeted Therapy. ,
Sci Rep
 3 , 2473 , 2013
Cells
Mammary tumor
Enzymes
Collagenase : 0.15%
Hyaluronidase : 0.020%
Medium
DMEM/F12
Mammary
Mouse
Mouse, 10 week
Cheng, N. and Lambert, D.
Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice. ,
J Vis Exp
 54 , e2716 , 2011
Cells
Fibroblasts, carcinoma
Enzymes
Trypsin : 0.2%
Hyaluronidase : 1,000 u/ml
Medium
DMEM
Mammary
Mouse
Mouse, 12 week
Taddei, I. , Deugnier, M. , Faraldo, M. , Petit, V. , Bouvard, D. , Medina, D. , Fassler, R. , Thiery, J. and Glukhova, M.
Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells ,
Nat Cell Biol
 10 , 716-22 , 2008
Cells
Mammary epithelial
Enzymes
Collagenase : 0.3%
Hyaluronidase : 100 u/ml
Trypsin : 0.25%
Medium
see reference
Mammary
Mouse
Mouse, 11 week
Mueller, S. , Clark, J. , Myers, P. and Korach, K.
Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
 143 , 2357 , 2002
Cells
Epithelial
Enzymes
Collagenase Type 3 : 25 u/ml
Hyaluronidase : 0.1%
Protease XIV : 0.05%
Medium
DMEM/F12
Mammary
Mouse
Mouse
Boulanger CA, Smith GH.
Reducing mammary cancer risk through premature stem cell senescence ,
Oncogene
 20 , 2264-72 , 2001
Cells
Mammary epithelial stem
Enzymes
Hyaluronidase : 0.1%
Pronase : 1.25%
Medium
DMEM
Mammary
Mouse
Mouse, BALB/C
Kanazawa, T. and Hosick, H.
Transformed Growth Phenotype of Mouse Mammary Epithelium in Primary Culture Induced by Specific Fetal Mesenchymes ,
J Cell Physiol
 153 , 381 , 1992
Cells
Epithelium
Enzymes
Collagenase : 250 u/ml
Medium
HBSS
Mammary
Mouse
Mouse, BALB/c, virgin, female, 4 month
Beck, J. , Hosick, H. and Watkins, B.
Growth of Epithelium From a Preneoplastic Mammary Outgrowth on Response to Mammary Adipose Tissue ,
In Vitro Cell Dev Biol
 25 (5) , 409 , 1989
Cells
Adipocytes
Enzymes
Trypsin : 50 µg/ml
Medium
DMEM
Mammary
Mouse
Mouse (BALB/cCrgl), mature
Bandyopadhyay, G. , Imagawa, W. , Wallace, D. and Nandi, S.
Proliferative effects of insulin and epidermal growth factor on mouse mammary epithelial cells in primary culture ,
J Biol Chem
 263 , 7567 , 1988
Cells
Epithelial
Enzymes
Pronase : 0.01%
Medium
Hepes buffered medium 199
Mammary
Mouse
Mouse, BALB/c, female, 6-8 week
Ehmann, U. , Peterson, W. and Misfeldt, D.
To Grow Mouse Mammary Epithelial Cells in Culture ,
J Cell Biol
 98 , 1026 , 1984
Cells
Epithelial
Enzymes
Medium
DMEM
Mammary
Mouse
Mouse (C3H/HeN), female
Taketani, Y. and Oka, T.
EGF Stimulates Cell Proliferation and Inhibits Functional Differentiation of Mouse Mammalian Dipithelial Cells in Culture ,
Endocrinology
 113 , 871 , 1983
Cells
Epithelial
Enzymes
Medium
Medium 199
Mammary
Mouse
Mouse, (BALB/c/Crgl Me), female, pregnant
Riser, M. , Huff, B. and Medina, D.
Pepsin Can Be Used To Subculture Viable Mammary Epithelial Cells ,
In Vitro
 19 , 730 , 1983
Cells
Epithelial
Enzymes
Pepsin : 0.1% and 0.05%
Pepsin : 0.1%
Medium
HBSS with 0.2% EDTA, CMF
Mammary
Mouse
Mouse, female
Jones, W. and Hallowes, R.
Isolation of the Epithelial Subcomponents of the Mouse Mammary Gland for Tissue-Level Culture Studies ,
J Tiss Cul Meth
 8 (1) , 17 , 1983
Cells
Epithelial
Enzymes
Medium
DMEM
Mammary
Mouse
Mouse (NMuMG), female, 2 months
Ehmann, U. and Misfeldt, D.
Mouse Mammary Cells in D-Valine Medium ,
In Vitro
 18 , 407 , 1982
Cells
Epithelial
Enzymes
Medium
DMEM
Mammary
Mouse
Mouse, BALB/cCr1, female
Asch, B. , Burstein, N. , Vidrich, A. and Sun, T.
Identification of Mouse Mammary Epithelial Cells by Immunofluorescence With Rabbit and Guinea-Pig Antikeratin Antisera ,
Proc Natl Acad Sci U S A
 78 , 5643 , 1981
Cells
Epithelial
Enzymes
Medium
HBSS/DMEM
Mammary
Mouse
Mouse (BALB/cfC3H)
Yang, J. , Guzman, R. , Richards, J. and Nandi, S.
Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels ,
In Vitro
 16 , 502 , 1980
Cells
Mammary tumors
Epithelial
Enzymes
Collagenase : 1.0%
Medium
HBSS
Mammary
Mouse
Mouse, pregnant 8-12 day
Yang J, Richards J, Guzman R, Imagawa W. and Nandi S.
Sustained growth in primary culture of normal mammary epithelial cells embedded in collagen gels ,
Proc Natl Acad Sci U S A
 77 , 2088 , 1980
Cells
Mammary
Medium
HBSS
Mammary
Mouse
Mouse (BALB/c), pregnant, 60-80 days
Ceriani, R. , Peterson, J. and Abraham, S.
Removal of Cell Surface Material by Enzymes Used to Dissociate Mammary Gland Cells ,
In Vitro
 14 , 887 , 1978
Cells
Mammary
Enzymes
Hyaluronidase : 0.1%
Medium
CMF medium
Mammary
Mouse
Mouse, BALB/cfC3H/Crgl, virgin, female
DeOme, K. , Miyamoto, M. , Osborn, R. , Guzman, R. and Lum, K.
Detection of Inaparent Nodule-transformed Cells in the Mammary Gland Tissues of Virgn Female BALB/cfC3H Mice ,
Cancer Res
 38 , 2103 , 1978
Cells
Nodule-transformed
Enzymes
Hyaluronidase : 0.1%
Medium
Medium 199
Mammary
Mouse
Mouse (BALB/cCrgl), virgin, female, 4-8 month
White, M. , Hu, A. , Hamamoto, S. and Nandi
In vitro analysis of proliferating epithelial cell populations from the mouse mammary gland: fibroblast-free growth and serial passage ,
In Vitro
 14 , 271 , 1978
Cells
Epithelial
Mammary gland
Enzymes
Medium
HBSS
Mammary
Mouse
Mouse, BALB/cCrgl, female
(also guinea-pig)
Asch, B. and Medina, D.
Concanavalin A-Induced Agglutinability of Normal, Preneoplastic, and Neoplastic Mouse Mammary Cells ,
J Natl Cancer Inst
 61 (6) , 1423 , 1978
Cells
Swiss 3T3
Enzymes
Medium
PBS CMF
Mammary
Mouse
Mouse, (C3H/Crgl or BALB/cCrgl), 8-10 day pregnant
Emerman, J. , Enami, J. , Pitelka, D. and Nandi, S.
Hormonal Effects on Intracellular and Secreted Casein in Cultures of Mouse Mammary Epithelial Cells on Floating Collagen Membranes ,
Proc Natl Acad Sci U S A
 74 (10) , 4466 , 1977
Cells
Epithelial
Enzymes
Medium
HBSS
Mammary
Mouse
Mouse, lactating
Kerkof, P. and Abraham, S.
Preparation of Adipose Cell-Free Suspensions of Mammary Gland Parenchymal Cells from Lactating Mice ,
Methods Enzymol
 69 , 693 , 1976
Cells
Parenchymal
Enzymes
Medium
Kreb's Ringer bicarbonate buffer
Mammary
Mouse
Mouse, female, early pregnancy <10 days
Moore, D. and Lasfargues, E.
Method for the Continuous Cultivation of Mammary Epithelium ,
In Vitro
 7 , 21 , 1971
Cells
Epithelial
Enzymes
Collagenase : 0.1%
Medium
Eagle's MEM
Mammary
Mouse
Mouse, CBA, virgin, 9/10 wk
Prop, F. and Wiepjes, G.
Improved method for preparation of single-cell suspensions from mammary glands of adult virgin mouse ,
Exp Cell Res
 61 , 451 , 1970
Cells
Mammary
Enzymes
Hyaluronidase : 0.1%
Medium
BSS, CMF
Mammary
Mouse
Mouse, lactating, 14-18 day
Pitelka, D. , Kerkof, P. , Gagne, H. , Smith, S. and Abraham, S.
Characteristics of Cells Dissociated from Mouse Mammary Glands. I. Method of Separation and Morphology of Parenchymal Cells from Lactating Glands ,
Exp Cell Res
 57 , 43 , 1969
Cells
Parenchymal
Enzymes
Collagenase : 0.33%
Medium
Kreb's buffer

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.