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Tissue References Search Tool

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Spleen
Mouse

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.
Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
 13 , e4679 , 2023
Cells
T cells
Enzymes
Medium
RPMI
Spleen
Mouse
Mouse, 4 week
Hou, M. , Han, J. , Li, G. , Kwon, M. , Jiang, J. , Emani, S. , Taglauer, E. , Park, J. , Choi, E. , Vodnala, M. , Fong, Y. , Emani, S. , Rosas, I. , Perrella, M. and Liu, X.
Multipotency of Mouse Trophoblast Stem Cells ,
Stem Cell Res Ther
 11 , 55 , 2020
Cells
Trophoblast
Enzymes
Medium
DMEM
Spleen
Mouse
Mouse
Villarreal, E. , Hewgley, W. , Lang, W. , Morton, C. , Mao, S. , Wu, J. and Sandoval, J.
In-bag Enzymatic Splenic Digestion: A Novel Alternative to Manual Morcellation? ,
J Surg Res
 218 , 209-216 , 2017
Cells
Speen
Enzymes
Medium
HBSS
Spleen
Mouse
Mouse
Klebanoff, C. , Spencer, S. , Torabi-Parizi, P. , Grainger, J. , Roychoudhuri, R. , Ji, Y. , Sukumar, M. , Muranski, P. , Scott, C. , Hall, J. , Ferreyra, G. , Leonardi, A. and Borman, Z. et al.
Retinoic Acid Controls the Homeostasis of Pre-cDC-Derived Splenic and Intestinal Dendritic Cells. ,
J Exp Med
 210 , 1961 , 2013
Cells
Splenic
Enzymes
Medium
PBS
Spleen
Mouse
Mouse
Yi, T. and Cyster, J.
EBI2-Mediated Bridging Channel Positioning Supports Splenic Dendritic Cell Homeostasis and Particulate Antigen Capture. ,
Elife
 2 , e00757 , 2013
Cells
Splenocytes
Enzymes
Medium
PBS
Spleen
Mouse
Mouse
Shi, C. , Jia, T. , Mendez-Ferrer, S. , Hohl, T. , Serbina, N. , Lipuma, L. , Leiner, I. , Li, M. , Frenette, P. and Pamer, E.
Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands. ,
Immunity
 34 , 590 , 2011
Cells
Spleen, bone marrow endothelial
Enzymes
Medium
PBS
Spleen
Mouse
Mouse
Flano, E. , Jewell, N. , Durbin, R. and Durbin, J.
Methods Used to Study Respiratory Virus Infection. , Curr Protoc Cell Biol Vol. Chapter 26 , Unit 26.3 , 2009
Cells
Dendritic
Enzymes
Medium
HBSS
Spleen
Mouse
Mouse, female, 6-8 week
Abou Fakher, F. , Rachinel, N. , Klimczak, M. , Louis, J. and Doyen, N.
TLR9-Dependent Activation of Dendritic Cells by DNA from Leishmania Major Favors Th1 Cell Development and the Resolution of Lesions. ,
J Immunol
 182 , 1386 , 2009
Cells
Dendritic
Enzymes
Medium
RPMI 1640
Spleen
Mouse
Mouse
Abe, K. , Nguyen, K. , Fine, S. , Mo, J. , Shen, C. , Shenouda, S. , Corr, M. , Jung, S. , Lee, J. , Eckmann, L. and Raz, E.
Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells. ,
Proc Natl Acad Sci U S A
 104 , 17022 , 2007
Cells
Dentritic
Enzymes
Collagenase : 300 u/ml
Medium
RPMI 1640
Spleen
Mouse
Mouse
Kang, H. , Liu, M. and Datta, S.
Low-dose Peptide Tolerance Therapy of Lupus Generates Plasmacytoid Dendritic Cells That Cause Expansion of Autoantigen-Specific Regulatory T Cells and Contraction of Inflammatory Th17 Cells ,
J Immunol
 178 , 7849-58 , 2007
Cells
Splenic dendritic
Enzymes
Medium
DMEM
Spleen
Mouse
Mouse
Mueller, S. , Matloubian, M. , Clemens, D, Sharpe, A. , Freeman, G. , Gangappa, S. , Larsen, C. and Ahmed, R.
Viral Targeting of Fibroblastic Reticular Cells Contributes to Immunosuppression and Persistence During Chronic Infection. ,
Proc Natl Acad Sci U S A
 104 , 15430 , 2007
Cells
Splenic
Enzymes
Medium
RPMI
Spleen
Mouse
Mouse
Benedict Chris A, De Trez Carl, Schneider Kirsten, Ha Sukwon, Patterson Ginelle, Ware Carl F.
Specific remodeling of splenic architecture by cytomegalovirus ,
PLoS Pathog
 2 , e16 , 2006
Cells
Splenic stromal
Enzymes
Collagenase Type 3 : 100-400 u/ml
Medium
HBSS
Spleen
Mouse
Mouse
Siragam, V. , Crow, A. , Brinc, D. , Song, S. , Freedman, J. and Lazarus, A.
Intravenous Immunoglobulin Ameliorates ITP via Activating Fc Gamma Receptors on Dendritic Cells. ,
Nat Med
 12 , 688 , 2006
Cells
Leukocytes
Enzymes
Collagenase Type 4 : 43 u/ml
Medium
RPMI 1640
Spleen
Mouse
Mouse
Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F. and Gabrieli, L
ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells ,
J Exp Med
 196 , 1415 , 2002
Cells
Dentritic
Enzymes
Medium
RPMI 1640
Spleen
Mouse
Mouse, 4-6 week
McLellan, A. , Kapp, M. , Eggert, A. , Linden, C. , Bommhardt, U. , Brocker, E. , Kammerer, U. and Kampgen, E.
Anatomic Location and T-cell Stimulatory Functions of Mouse Dendritic Cell Subsets Defined by CD4 and CD8 Expression ,
Blood
 99 , 2084-93 , 2002
Cells
Dentritic
Enzymes
Medium
RPMI-1640
Spleen
Mouse
Mouse
Stagg, AJ, Burke, F, Hill, S. and Knight, S.
Isolation of Mouse Spleen Dendritic Cells. ,
Methods Mol Med
 64 , 9-22 , 2001
Cells
Dentritic cells
Enzymes
Medium
HEPES/RPMI 1640
Spleen
Mouse
Mouse
Brasel K, De Smedt T, Smith JL, Maliszewski CR.
Generation of murine dendritic cells from flt3-ligand-supplemented bone marrow cultures ,
Blood
 96 , 3029-39 , 2000
Cells
Dentritic
Enzymes
Collagenase : 100 u/ml
Medium
HBSS
Tumor
Mouse

Mouse

Nahar, S. , Huang, Y. , Nagy, B. , Zebala, J. , Maeda, D. , Rudloff, U. , Oppenheim, J. and And, D
Regression and Eradication of Triple-Negative Breast Carcinoma in 4T1 Mouse Model by Combination Immunotherapies. , Cancers (Basel) Vol. 15 , 2023
Cells
Tumor
Enzymes
Tumor
Mouse
Mouse
Walker, S. , Wankell, M. , Ho, V. , White, R. , Deo, N. , Devine, C. , Dewdney, B. , Bhathal, P. , Govaere, O. , Roskams, T. , Qiao, L. , George, J. and Hebbard, L.
Targeting mTOR and Src Restricts Hepatocellular Carcinoma Growth in a Novel Murine Liver Cancer Model. ,
PLoS ONE
 14 , e0212860 , 2019
Cells
liver tumor
Enzymes
Medium
HBSS
Tumor
Mouse
Mouse, adult
Neal, J,, Li ,X. , Zhu, J. , Giangarra, V. , Grzeskowiak, C. , Ju, J. , Liu, I. Chiou, S. , Salahudeen, A. and Smith, A. et al
Organoid Modeling of the Tumor Immune Microenvironment. ,
Cell
 175 , 1972 , 2018
Cells
Organoids
Enzymes
Collagenase Type 4 : 200-300 u/ml
Medium
DMEM
Tumor
Mouse
Mouse
Erdreich-Epstein, A. , Singh, A. , Joshi, S. , Vega, F. , Guo, P. , Xu, J. , Groshen, S. , Ye, W. , Millard, M. , Campan, M. , Morales, G. , Garlich, J. , Laird, P. , Seeger, R. , Shimada, H. and Durden, D.
Association of High Microvessel A,B and Low PTEN with Poor Outcome in Stage 3 Neuroblastoma: Rationale for Using First in Class Dual PI3K/BRD4 Inhibitor, SF1126. ,
Oncotarget
 8 , 52193-52210 , 2017
Cells
Tumor derived neutroblastoma
Enzymes
Papain : 10 u/ml
Deoxyribonuclease I : 250 u/ml
Medium
DPBS
Tumor
Mouse
Mouse
Boyd, N. , Walker, K. , Fried, J. , Hackney, J. , McDonald, P. , Benavides, G. , Spina, R. , Audia, A. , Scott, S. , Libby, C. , Tran, A. , Bevensee, M. , Griguer, C. , Nozell, S. , Gillespie, G. and Nabors, B.
Addition of Carbonic Anhydrase 9 Inhibitor SLC-0111 to Temozolomide Treatment Delays Glioblastoma Growth In Vivo. ,
JCI Insight
 2 , 2017
Cells
Glioblastoma
Enzymes
PDS kit : per instructions
Medium
DMEM/F12
Tumor
Mouse
Mouse
Aguilera, T. , Rafat, M. , Castellini, L. , Shehade, H. , Kariolis, M. , Hui, A. , Stehr, H. , von Eyben, R. , Jiang, D. , Ellies, L. , Koong, A. , Diehn, M. , Rankin, E. Graves, E. and Giaccia, A.
Reprogramming the Immunological Microenvironment through Radiation and Targeting Axl. ,
Nat Commun
 7 , 13898 , 2016
Cells
Tumor
Enzymes
Collagenase : 0.1%
Neutral Protease : 0.5 u/ml
Medium
F12K
Tumor
Mouse
Mouse
Otvos, B. , Silver, D. , Mulkearns-Hubert, E. , Alvarado, A. , Turaga, S. , Sorensen, M. , Rayman, P. , Flavahan, W. , Hale, J. , Stoltz, K. , Sinyuk, M. , Wu, Q. , Jarrar, A. , Kim, S. , Fox, P. , Nakano, I. and Rich, J.
Cancer Stem Cell-Secreted Macrophage Migration Inhibitory Factor Stimulates Myeloid Derived Suppressor Cell Function and Facilitates Glioblastoma Immune Evasion. ,
Stem Cells
 34 , 2026-39 , 2016
Cells
Glioblastoma
Enzymes
PDS kit : per instructions
Medium
Neurobasal
Tumor
Mouse
Mouse
Filtjens, J. , Keirsse, J. , Van Ammel, E. , Taveirne, S. , Van Acker, A. , Kerre, T. , Taghon, T. , Vandekerckhove, B. , Plum, J. , Van Ginderachter, J. and Leclercq, G.
Expression of the Inhibitory Ly49E Receptor is Not Critically Involved in the Immune Response Against Cutaneous, Pulmonary or Liver tumours. ,
Sci Rep
 6 , 30564 , 2016
Cells
Tumor
Enzymes
Medium
RPMI-1640
Tumor
Mouse
Mouse
Singer, M. , Wang, C. , Cong, L. , Marjanovic, N. , Kowalczyk, Mo. , Zhang, H. , Nyman, J. , Sakuishi, K. , Kurtulus, S. , Gennert, D. , Xia, J. , Kwon, J. , Nevin, J. , Herbst, R. , Yanai, I. , Rozenblatt-Rosen, O. and Kuchroo, V.
A Distinct Gene Module for Dysfunction Uncoupled from Activation in Tumor-Infiltrating T Cells. ,
Cell
 166 , 1500-1511.e9 , 2016
Cells
Tumor-infiltrating lymphocytes
Enzymes
Medium
DMEM
Tumor
Mouse
Mouse
Hosseini, H. , Obradovic, M. , Hoffmann, M. , Harper, K. , Sosa, M. , Werner-Klein, M. , Nanduri, L. , Werno, C. , Ehrl, C. , Maneck, M. , Patwary, N. , Haunschild, G. , Guzvic, M. and Reimelt, C. et al.
Early Dissemination Seeds Metastasis in Breast Cancer. ,
Nature
 , 2016
Cells
Mammary tumor
Enzymes
Collagenase Type 1 : 200 u/ml
Hyaluronidase : 0.001%
Medium
DMEM/F12
Tumor
Mouse
Mouse
Blanco, S. , Bandiera, R. , Popis, M. , Hussain, S. , Lombard, P. , Aleksic, J. , Sajini, A. , Tanna, H. , Cortes-Garrido, R. , Gkatza, N. , Dietmann, S. and Frye, M.
Stem Cell Function and Stress Response are Controlled by Protein Synthesis. ,
Nature
 534 , 335-40 , 2016
Cells
Keratinocytes
Enzymes
Medium
Low calcium containing media
Tumor
Mouse
Mouse
Kitajima, S. , Kohno, S. , Kondoh, A. , Sasaki, N. , Nishimoto, Y. , Li, F. , Mohammed, M. , Muranaka, H. , Nagatani, N. , Suzuki, M. , Kido, Y. and Takahashi, C.
Undifferentiated State Induced by Rb-p53 Double Inactivation in Mouse Thyroid Neuroendocrine Cells and Embryonic Fibroblasts. ,
Stem Cells
 33 , 1657-69 , 2015
Cells
Thyroid tumor
Enzymes
Collagenase Type 1 : 300 u/ml
Hyaluronidase : 100 u/ml
Medium
DMEM
Tumor
Mouse
Mouse
Xiao, L. , McCann, J. and Dudley, A.
Isolation and Culture Expansion of Tumor-specific Endothelial Cells. ,
J Vis Exp
 , e53072 , 2015
Cells
Endothelial
Enzymes
Medium
DMEM
Tumor
Mouse
Mouse
Ali, O. , Verbeke, C. , Johnson, C. , Sands, R. , Lewin, S. , White, D. , Doherty, E. , Dranoff, G. and Mooney, D.
Identification of Immune Factors Regulating Antitumor Immunity Using Polymeric Vaccines with Multiple Adjuvants. ,
Cancer Res
 74 , 1670-81 , 2014
Cells
Tumor
Enzymes
Collagenase Type 1 : 250 u/ml
Medium
DMEM
Tumor
Mouse
Mouse
Devaud, C. , Westwood, J. , John, L. , Flynn, J. , Paquet-Fifield, S. , Duong, C. , Yong, C. , Pegram, H. , Stacker, S. , Achen, M. Stewart, T. , Snyder, L. , Teng, M. , Smyth, M. , Darcy, P. and Kershaw, M
Tissues in Different Anatomical Sites can Sculpt and Vary the Tumor Microenvironment to Affect Responses to Therapy. ,
Mol Ther
 22 , 18-27 , 2014
Cells
Tumor
Enzymes
Medium
PBS
Tumor
Mouse
Mouse
Dunleavey, J. , Xiao, L. , Thompson, J. , Kim, M. , Shields, J. , Shelton, S. , Irvin, D. , Brings, V. , Ollila, D. , Brekken, R. , Dayton, P. , Melero-Martin, J. and Dudley, A.
Vascular Channels Formed by Subpopulations of PECAM1+ Melanoma Cells. ,
Nat Commun
 5 , 5200 , 2014
Cells
Tumor
Enzymes
Medium
DMEM
Tumor
Mouse
Mouse
Liu, X. , Johnson, S. , Liu, S. , Kanojia, D. , Yue, W. , Singh, U. , Wang, Q, Wang Qi, Nie, Q. and Chen H.
Nonlinear Growth Kinetics of Breast Cancer Stem Cells: Implications for Cancer Stem Cell Targeted Therapy. ,
Sci Rep
 3 , 2473 , 2013
Cells
Mammary tumor
Enzymes
Collagenase : 0.15%
Hyaluronidase : 0.020%
Medium
DMEM/F12
Tumor
Mouse
Mouse
Kazerounian, S. , Gerald, D. , Huang, M. , Chin, R, Udayakumar, D, Zheng, N. , O'Donnell, R. , Perruzzi, C. , Mangiante, L. , Pourat, J. , Phung, T. , Bravo-Nuevo, A. , Shechter, S. , McNamara, S. and Duhadaway, K.
RhoB Differentially Controls Akt Function in Tumor Cells and Stromal Endothelial Cells During Breast Tumorigenesis. ,
Cancer Res
 73 , 50 , 2013
Cells
Tumor endothelial
Enzymes
Medium
HBSS
Tumor
Mouse
Mouse
Chen, X. , Stewart, E. , Shelat, A. , Qu, C. , Bahrami, A. , Hatley, M. , Wu, G. , Bradley, C. , McEvoy, J. , Pappo, A. , Spunt, S. , Valentine, M. , Valentine, V. , Krafcik, F. , Lang, W. , Wierdl, M. and Tsurkan, L.
Targeting Oxidative Stress in Embryonal Rhabdomyosarcoma. ,
Cancer Cell
 24 , 710-24 , 2013
Cells
Rhabdomycosarcoma tumor
Enzymes
Trypsin : 0.012%
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.