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Tissue References Search Tool

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Colon
Mouse
Mouse
Larmonier, C. , Shehab, K. , Laubitz, D. , Jamwal, D. , Ghishan, F. and Kiela, P.
Transcriptional Reprogramming and Resistance to Colonic Mucosal Injury in Poly(ADP-ribose) Polymerase 1 (PARP1)-deficient Mice. ,
J Biol Chem
 291 , 8918-30 , 2016
Cells
Lamina propria
Enzymes
Collagenase : 100 u/ml
Medium
RPMI 1640
Colon
Mouse
Mouse
Fattahi, F. , Steinbeck, J. , Kriks, S. , Tchieu, J. , Zimmer, B. , Kishinevsky, S. , Zeltner, N. , Mica, Y. , El-Nachef, W. , Zhao, H. , de Stanchina, E. , Gershon, M. , Grikscheit, T. , Chen, S. and Studer, L.
Deriving Human ENS Lineages for Cell Therapy and Drug Discovery in Hirschsprung Disease. ,
Nature
 531 , 105-9 , 2016
Cells
Colon organoid
Enzymes
Collagenase Type 1 : 800 u/ml
Neutral Protease : 0.013%
Medium
DMEM
Colon
Mouse
Mouse, 8-10 week
Round, J. and Mazmanian, S.
Inducible Foxp3+ Regulatory T-cell Development by a Commensal Bacterium of the Intestinal Microbiota. ,
Proc Natl Acad Sci U S A
 107 , 12204-9 , 2010
Cells
Lamina propria lymphocytes
Enzymes
Medium
HBSS
Colon
Mouse
Mouse
Atarashi, K. , Nishimura, J. , Shima, T. , Umesaki, Y. , Yamamoto, M. , Onoue, M. , Yagita, H. , Ishii, N. , Evans, R. , Honda, K. and Takeda, K.
ATP Drives Lamina Propria T(H)17 Cell Differentiation. ,
Nature
 455 , 808 , 2008
Cells
Lamina propria
Enzymes
Medium
RPMI 1640
Colon
Mouse
Mouse
Weigmann Benno, Tubbe Ingrid, Seidel Daniel, Nicolaev Alex, Becker Christoph, Neurath Markus F.
Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue ,
Nat Protoc
 2 , 2307-11 , 2007
Cells
Lamina propria mononuclear cells
Enzymes
Medium
HBSS
Colon
Mouse
Mouse
Abe, K. , Nguyen, K. , Fine, S. , Mo, J. , Shen, C. , Shenouda, S. , Corr, M. , Jung, S. , Lee, J. , Eckmann, L. and Raz, E.
Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells. ,
Proc Natl Acad Sci U S A
 104 , 17022 , 2007
Cells
Dentritic
Enzymes
Collagenase : 300 u/ml
Medium
RPMI 1640
Colon
Mouse
Mouse, 6-8 week
Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F.
Phenotypic characterization of human colorectal cancer stem cells ,
Proc Natl Acad Sci U S A
 104 , 10158-63 , 2007
Cells
Cancer stem cell
Enzymes
Collagenase Type 3 : 200 u/ml
Deoxyribonuclease I : 100 u/ml
Medium
RPMI-1640
Colon
Mouse
Mouse
Annacker O, Coombes JL, Malmstrom V, Uhlig HH, Bourne T, Johansson-Lindbom B, Agace WW, Parker CM, Powrie F.
Essential role for CD103 in the T cell-mediated regulation of experimental colitis ,
J Exp Med
 202 , 1051 , 2005
Cells
Lympocytes
Enzymes
Collagenase/Dispase : 100 u/ml
Medium
RPMI 1640
Colon
Mouse
Mouse, 6-8 week
Totsuka T, Kanai T, Uraushihara K, Iiyama R, Yamazaki M, Akiba H, Yagita H, Okumura K, Watanabe M.
Therapeutic effect of anti-OX40L and anti-TNF-alpha MAbs in a murine model of chronic colitis ,
Am J Physiol/Gastro
 284 , G595-603 , 2003
Cells
Lamina propria lymphocytes
Enzymes
Medium
HBSS
Colon
Mouse
Mouse, 6-8 week
Wirtz S. , Becker C. , Blumberg R. , Galle P. and Neurath M.
Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA ,
J Immunol
 168(1) , 411 , 2002
Cells
Lamina propria mononuclear cells
Enzymes
Medium
RPMI
Organoids
Mouse

Mouse

Fujimura, T. , Enomoto, Y. , Katsura, H. , Ogawa, T. , Baba, S. , Ogata, A. , Yamaoka, A. , Shiroguchi, K. and Morimoto, M.
Identifying a Lung Stem Cell Subpopulation by Combining Single-Cell Morphometrics, Organoid Culture, and Transcriptomics. ,
Stem Cells
 41 , 809-820 , 2023
Cells
Lung
Enzymes
Collagenase Type 1 : 450 u/ml
Elastase : 1 u/ml
Neutral Protease : 5 u/ml
Medium
DMEM/F12
Organoids
Mouse

Mouse

Klemke, L. , Blume, J. , Oliveira, T. and Schulz-Heddergott, R.
Preparation and Cultivation of Colonic and Small Intestinal Murine Organoids Including Analysis of Gene Expression and Organoid Viability. ,
Bio Protoc
 12 , e4298 , 2022
Cells
Colonic tumor organoids
Enzymes
Medium
PBS
Organoids
Mouse

Mouse

Huang, L. , Desai, R. , Conrad, D. , Leite, N. , Akshinthala, D. , Lim, C. , Gonzalez, R. , Muthuswamy, L. , Gartner, Z. and Muthuswamy, S.
Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids. , Cell Stem Cell Vol. 28 , 1090-1104.e6 , 2021
Cells
Organoid cultures
Enzymes
Stemxyme 1 : 0.1%
Medium
PBS
Organoids
Mouse

Mouse

Charifou, E. , Sumbal, J. , Koledova, Z. , Li, H. and Chiche, A.
A Robust Mammary Organoid System to Model Lactation and Involution-like Processes. ,
Bio Protoc
 11 , e3996 , 2021
Cells
Mammary organoids
Enzymes
Trypsin : 0.2%
Medium
DMEM/F12
Organoids
Mouse

Mouse, adult

Neal, J,, Li ,X. , Zhu, J. , Giangarra, V. , Grzeskowiak, C. , Ju, J. , Liu, I. Chiou, S. , Salahudeen, A. and Smith, A. et al
Organoid Modeling of the Tumor Immune Microenvironment. ,
Cell
 175 , 1972 , 2018
Cells
Organoids
Enzymes
Collagenase Type 4 : 200-300 u/ml
Medium
DMEM
Organoids
Mouse

Mouse, embryonic

Nikolić, M. , Caritg, O. , Jeng, Q. , Johnson, J. , Sun, D. , Howell, K. , Brady, J. , Laresgoiti, U. , Allen, G. , Butler, R. , Zilbauer, M. , Giangreco, A. and Rawlins, E.
Human Embryonic Lung Epithelial Tips are Multipotent Progenitors that can be Expanded In vitro as Long-term Self-renewing Organoids. ,
Elife
 6 , 2017
Cells
Lung organoids
Enzymes
Medium
DMEM/F12
Organoids
Mouse

Mouse

O'Rourke, K. , Ackerman, S. , Dow, L. and Lowe, S.
Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells. ,
Bio Protoc
 6 , 2016
Cells
Intestinal organoids
Enzymes
Medium
Basal Medium
Organoids
Mouse

Mouse, 8-12 week

Huch, M. , Bonfanti, P. , Boj, S. , Sato, T. , Loomans, C. , Van de Wetering, M. , Sojoodi, M. , Li, V. , Schuijers, J. , Gracanin, A. , Ringnalda, F. , Begthel, H. , Hamer, K. , Mulder, J. , Van Es, J. and De Koning, E.
Unlimited In Vitro Expansion of Adult Bi-Potent Pancreas Progenitors Through the Lgr5/R-Spondin Axis. ,
EMBO J
 32 , 2708-21 , 2013
Cells
Pancreas organoid
Enzymes
Neutral Protease : 0.012%
Collagenase : 0.012%
Medium
DMEM
Organoids
Mouse

Mouse

Xue, X. and Shah, Y.
In Vitro Organoid Culture of Primary Mouse Colon Tumors. ,
J Vis Exp
 , e50210 , 2013
Cells
Colon tumor organoids
Enzymes
Medium
DMEM
Organoids
Mouse

Mouse

Barthel, E. , Speer, A. , Levin, D. , Sala, F. , Hou, X. , Torashima, Y. , Wigfall, C. and Grikscheit, T.
Tissue Engineering of the Intestine in a Murine Model. ,
J Vis Exp
 70 , e4279 , 2012
Cells
Intestinal organoids
Enzymes
Collagenase Type 1 : 800 u/ml
Neutral Protease : 0.013%
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.