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Tissue References Search Tool

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Muscle
Porcine
Porcine, postnatal
Miersch, C. , Stange, K. and Rontgen, M.
Effects of Trypsinization and of a Combined Trypsin, Collagenase, and DNase Digestion on Liberation and in Vitro Function of Satellite Cells Isolated from Juvenile Porcine Muscles. ,
In Vitro Cell Dev Biol Anim
 54 , 406-412 , 2018
Cells
Muscle satellite
Enzymes
Medium
Eagle MEM
Muscle
Porcine
Porcine, 2 month
Lewis, F. , Henning, B. , Marazzi, G. , Sassoon, D. , Ellison, G. and Nadal-Ginard, B.
Porcine Skeletal Muscle-Derived Multipotent PW1pos/Pax7neg Interstitial Cells: Isolation, Characterization, and Long-Term Culture. ,
Stem Cells Transl Med
 3 , 702-12 , 2014
Cells
Skeletal muscle
Enzymes
Medium
HBSS
Muscle
Porcine
Porcine, male, 25-40 kg
Korzick, D. , Laughlin, M. and Bowles, D.
Alterations in PKC signaling underlie enhanced myogenic tone in exercise-trained porcine coronary resistance arteries ,
J Appl Physiol
 96 , 1425-32 , 2004
Cells
Coronary myocytes
Enzymes
Medium
low calcium physiological saline
Muscle
Porcine
Porcine, adult, 35-45 kg
Wamhoff BR, Dixon JL, Sturek M.
Atorvastatin treatment prevents alterations in coronary smooth muscle nuclear Ca2+ signaling in diabetic dyslipidemia ,
J Vasc Res
 39 , 208 , 2002
Cells
Arterial smooth muscle
Enzymes
Collagenase Type 2 : 294 u/ml
Elastase : 6.5 u/ml
Deoxyribonuclease I : 0.4 mg/ml
Medium
MEM
Muscle
Porcine
Porcine, Yorkshire, 30kg
Sirous ZN, Fleming JB, Khalil RA.
Endothelin-1 enhances eicosanoids-induced coronary smooth muscle contraction by activating specific protein kinase C isoforms ,
Hypertension
 37 , 497-504 , 2001
Cells
Coronary smooth muscle
Enzymes
Collagenase Type 2 : 150 u/ml
Elastase : 0.05%
Medium
HBSS
Muscle
Porcine
Porcine
Shieh CC, Feng J, Buckner SA, Brioni JD, Coghlan MJ, Sullivan JP, Gopalakrishnan M.
Functional implication of spare ATP-sensitive K(+) channels in bladder smooth muscle cells ,
J Pharmacol Exp Ther
 296 , 669-75 , 2001
Cells
Bladder smooth muscle
Enzymes
Collagenase Type 2 : 0.1-0.2%
Medium
Krebs-Ringer bicarbonate
Muscle
Porcine
Porcine, juvenile, 30kg
Huckle WR, Drag MD, Acker WR, Powers M, McFall RC, Holder DJ, Fujita T, Stabilito II, Kim D, Ondeyka DL, Mantlo NB, Chang RS, Reilly CF, Schwartz RS, Greenlee WJ, Johnson RG.
Effects of subtype-selective and balanced angiotensin II receptor antagonists in a porcine coronary artery model of vascular restenosis ,
Circulation
 93 , 1009-19 , 1996
Cells
Coronary smooth muscle cells
Enzymes
Collagenase : 0.3%
Elastase : 0.05%
Medium
HBSS
Muscle
Porcine
Porcine
Xiong, Yimin, Xu, Shangzhe. and Slakey, Linda L
Modulation of Response to Adenosine in Vascular Smooth Muscle Cells Cultured in Defined Medium ,
In Vitro Cell Dev Biol
 27 , 355 , 1991
Cells
Smooth muscle, aorta
Enzymes
Collagenase : 0.3%
Medium
DMEM
Muscle
Porcine
Porcine
Breton, M. , Berrou, E. , Deudon, E. and Picard, J.
Changes in Proteoglycans of Cultured Pig Aortic Smooth Muscle Cells During Subculture ,
In Vitro Cell Dev Biol
 26 , 157 , 1990
Cells
Smooth muscle
Aorta
Enzymes
Trypsin : 0.05%
Medium
EDTA 0.02%
Muscle
Porcine
Porcine
Fehr, T. , Dickinson, E. , Goldman, S. and Slakey, L.
Cyclic AMP Efflux is Regulated by Occupancy of The Adenosine Receptor in Pig Aortic Smooth Muscle Cells ,
J Biol Chem
 265 , 10974 , 1990
Cells
Smooth muscle
Aortic medial tissue
Enzymes
Collagenase : 0.30%
Medium
DMEM
Muscle
Rat
Rat, day 7
Vilmont, V. , Cadot, B. , Ouanoumou, G. and Gomes, E.
A System for Studying Mechanisms of Neuromuscular Junction Development and Maintence ,
Development
 143 , 2464 , 2016
Cells
Skeletal muscle
Enzymes
Collagenase : 0.05%
Medium
DMEM
Muscle
Rat
Rat, SD
Zeng, Y. , Liu, H. , Kang, K. , Wang, Z. , Hui, G. , Zhang, X. , Zhong, J. , Peng, W. , Ramchandran, R. , Raj, J. and Gou, D.
Hypoxia Inducible Factor-1 Mediates Expression of miR-322: Potential Role in Proliferation and Migration of Pulmonary Arterial Smooth Muscle Cells. ,
Sci Rep
 5 , 12098 , 2015
Cells
Pulmonary arterial smooth muscle
Enzymes
Elastase : 0.05%
Medium
Buffer
Muscle
Rat
Rat
Weisleder, N. , Zhou, J. and Ma, J.
Detection of Calcium Sparks in Intact and Permeabilized Skeletal Muscle Fibers. ,
Methods Mol Biol
 798 , 395 , 2012
Cells
Skeletal muscle fiber
Enzymes
Medium
Tyrode
Muscle
Rat
Rat
Weber, S. , Gratopp, A. , Akanbi, S. , Rheinlaender, C. , Sallmon, H. , Barikbin, P. and Koehne, P.
Isolation and Culture of Fibroblasts, Vascular Smooth Muscle, and Endothelial Cells From the Fetal Rat Ductus Arteriosus ,
Pediatr Res
 70 , 236 , 2011
Cells
Vascular smooth muscle
Enzymes
Medium
DMEM
Muscle
Rat
Rat, fetal 17-18 day
Das, M. , Rumsey, J. , Bhargava, N. , Stancescu, M. and Hickman, J.
Skeletal Muscle Tissue Engineering: A Maturation Model Promoting Long-Term Survival of Myotubes, Structural Development of the Excitation-Contraction Coupling Apparatus and Neonatal Myosin Heavy Chain Expression. ,
Biomaterials
 30 , 5392 , 2009
Cells
Myotubes
Enzymes
Trypsin : 0.05%
Medium
see reference
Muscle
Rat
Rat
Pacak, C. and Cowan, D.
Fabrication of Myogenic Engineered Tissue Constructs. ,
J Vis Exp
 27 , e1137 , 2009
Cells
Myogenic
Enzymes
Medium
Ham's F-10
Muscle
Rat
Rat
Larkin, L. , Calve, S. , Kostrominova, T. and Arruda, E.
Structure and Functional Evaluation of Tendon-Skeletal Muscle Constructs Engineered In Vitro. ,
Tiss Eng
 12 , 3149-58 , 2006
Cells
myogenic
Enzymes
Neutral Protease : 8 u/ml
Collagenase Type 4 : 200 u/ml
Medium
DMEM
Muscle
Rat
Rat, Lewis, neonatal
Kim, J. , Hadlock, T. , Cheney, M. , Varvares, M. and Marler, J.
Muscle Tissue Engineering for Partial Glossectomy Defects. ,
Arch Facial Plast Surg
 5 , 403 , 2003
Cells
Myoblasts
Enzymes
Medium
Ham's F-10
Muscle
Rat
Rat, SD, adult and neonatal w/in 1 day of birth
Dennis, R. , Kosnik II, P. , Gilbert, M. and Faulkner, J.
Excitability and Contractility of Skeletal Muscle Engineered from Primary Cultures and Cell Lines ,
Am J Physiol Cell Physiol
 280 , C288 , 2001
Cells
Myooids
Enzymes
Neutral Protease : 4 u/ml
Medium
Ham's F-12
Muscle
Rat
Rat, SD, 250 g
Jaggar JH.
Intravascular pressure regulates local and global Ca(2+) signaling in cerebral artery smooth muscle cells ,
Am J Physiol Cell Physiol
 281 , C439-48 , 2001
Cells
Arterial smooth muscle
Enzymes
Papain : 0.03%
Collagenase : 0.1%
Medium
see reference
Muscle
Rat
Rat, SD, 3 month
Stevenson S. , Rollence M. , Marshall-Neff J. and Liau G.
A Genetically Modified Adenoviral Vector Exhibits Enhanced Gene Transfer of Human Smooth Muscle Cells ,
J Vasc Res
 38(5) , 471 , 2001
Cells
Smooth muscle cells
Enzymes
Medium
M-199
Muscle
Rat
Rat, Wistar, adult, male
Wellman, G. , Barrett-Jolley, R. , Koppel, H. Everitt, D. and Quayle, J.
Inhibition of Vascular KATP Channels by U-37883A: A Comparison with Cardiac and Skeletal Muscle ,
Br J Pharmacol
 128 , 909 , 1999
Cells
Smooth & skeletal muscle
Cardiac myocytes
Enzymes
Protease : 0.01%
Medium
PSS
Muscle
Rat
Rat, SD, male, 250-350 g
Hrometz, S. , Edelmann, S. , McCune, D. , Olges, J. , Hadley, R. , Perez ,D. and Piascik, M.
Expression of Multiple Alpha1-Adrenoceptors on Vascular Smooth Muscle: Correlation with the Regulation of Contraction ,
J Pharmacol Exp Ther
 290(1) , 452 , 1999
Cells
Vascular smooth muscle
Enzymes
Elastase : 0.0125%
Medium
DMEM
Muscle
Rat
Rat, SD, male, 150-175 g
Redmond, E. , Cahill, P. and Sitzmann, J.
Perfused Transcapillary Smooth Muscle and Endothelial Cell Co-Culture-A Novel In Vitro Model ,
In Vitro Cell Dev Biol Anim
 31 , 601 , 1995
Cells
Smooth muscle, endothelial
Enzymes
Trypsin : 0.04%
Medium
MEM
Muscle
Rat
Rat, Wistar Kyoto, 10-15 weeks
McGuire, P. , Walker-Caprioglio, H. , Little, S. and McGuffee, L.
Isolation and Culture of Rat Superior Mesenteric Artery Smooth Muscle Cells ,
In Vitro Cell Dev Biol
 29 , 135 , 1993
Cells
Smooth muscle, mesenteric artery
Enzymes
Trypsin : 0.05%
Medium
MEM
Muscle
Rat
Rat
(also rabbit, guinea-pig)
Hu, S. and Kim, H.
Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid ,
FASEB J
 6 , A383 , 1992
Cells
Smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Potassium buffer solution
Muscle
Rat
Rat, SD, 19 days
Loch-Caruso, R. , Pahl, M. and Juberg, D.
Rat Myometrial Smooth Muscle Cells Show High Levels of Gap Junctional Communication Under a Variety of Culture Conditions ,
In Vitro Cell Dev Biol
 28 , 97 , 1992
Cells
Smooth muscle, myometrial
Enzymes
Trypsin : 150 µg/ml
Medium
HBSS or PSS, CMF
Muscle
Rat
Rat, SHRs and WKY, male, 10 - 14 weeks
Bolzon, B. and Cheung, D.
Isolation and Characterization of Single Vascular Smooth Muscle Cells From Spontaneously Hypertensive Rats ,
Hypertension
 14 , 137 , 1989
Cells
Smooth muscle, tail arteries
Enzymes
Papain : 0.1%
Medium
HEPES buffer
(see reference)
Muscle
Rat
Rat, 1-3 day
Barone, L. , Wolfe, L. , Faris, B. and Franzblau, C.
Elastin mRNA Levels and Insoluble Elastin Accumulation in Neonatal Rat ,
Biochemistry
 27 , 3175 , 1988
Cells
Smooth muscle, aortic
Enzymes
Elastase : 0.0125%
Medium
DMEM
Muscle
Rat
Rat
Bean, B. , Sturek, M. , Puga, A. and Hermsmeyer, K.
Calcium Channels in Muscle Cells Isolated From Rat Mesenteric Arteries: Modulation by Dihydropyridine Drugs ,
Circ Res
 59 , 229 , 1986
Cells
Muscle, mesenteric arteries
Enzymes
Trypsin : 0.05%
Medium
HEPES KG solution
(see reference)
Muscle
Rat
Rat, SHR, WKY. either sex, 12-19 day, 3 month, and retired breeders
Gordon, D. , Mohai, L. and Schwartz, S.
Induction of Polyploidy in Cultures of Neonatal Rat Aortic Smooth Muscle Cells ,
Circ Res
 59 , 633 , 1986
Cells
Endothelial, aortic
Enzymes
Elastase : 0.05%
Medium
Waymouth's culture medium
Muscle
Rat
Rat, male, 150-250 g
Cole, O. , Fan, T. and Lewis, G.
Isolation, Characterization, Growth and Culture of Endothelial Cells From the Rat Aorta ,
Cell Biol Int Rep
 10 (6) , 399 , 1986
Cells
Endothelial, aortic
Medium
RPMI 1640
Muscle
Rat
Rat, SD, male, 200 - 250 g
Brock, T. , Alexander, R. , Ekstein, L. , Atkinson, W. and Gimbrone, M.
Angiotensin Increases Cytosolic Free Calcium in Cultured Vascular Smooth Muscle Cells ,
Hypertension
 7 , 105 , 1985
Cells
Smooth muscle, thoracic aorta
Enzymes
Trypsin : 0.0375%
Medium
Eagle's MEM with calcium
Muscle
Rat
Rat, Wistar, female, 10 weeks
Boulanger-Saunier, C. , Kattenburg, D. and Stoclet, J.
Cyclic AMP-dependent Phosphorylation of a 16kDa Protein in a Plasma Membrane-enriched Fraction of Rat Aortic Myocytes ,
F.E.B.S. Lett.
 193 , 283 , 1985
Cells
Myocytes
Enzymes
Collagenase : 0.1%
Medium
HBSS
Muscle
Rat
Rat, SD, 225-250g
Gunther S, Alexander RW, Atkinson WJ. and Gimbrone MA Jr.
Functional angiotensin II receptors in cultured vascular smooth muscle cells ,
J Cell Biol
 92 , 289 , 1982
Cells
Mesenteric artery smooth muscle cells
Enzymes
Medium
HBSS
Muscle
Rat
Rat, 3-4 day
Kasten, F.
Rat Myocardial Cells In Vitro: Mitosis and Differentiated Properties ,
In Vitro
 8 , 128 , 1972
Cells
Myocardial
Enzymes
Trypsin NF 1:250 : 0.125%
Medium
HBSS CMF

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.