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Tissue References Search Tool

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Adipose/Fat
Equine
Equine
Vidal, M. , Robinson, S. , Lopez, M. , Paulsen, D. , Borkhsenious, O. , Johnson, J. , Moore, R. and Gimble, J.
Comparison of Chondrogenic Potential in Equine Mesenchymal Stromal Cells Derived from Adipose Tissue and Bone Marrow. ,
Vet Surg
 37 , 713 , 2008
Cells
Adipose derived stem cells
Enzymes
Medium
PBS
Adipose/Fat
Equine
Equine, 1-5 year
Vidal, M. , Kilroy, G. , Lopez, M. , Johnson, J. , Moore, R. and Gimble, J.
Characterization of Equine Adipose Tissue-Derived Stromal Cells: Adipogenic and Osteogenic Capacity and Comparison with Bone Marrow-Derived Mesenchymal Stromal Cells. ,
Vet Surg
 36 , 613 , 2007
Cells
Adipose derived stem cells
Enzymes
Medium
PBS
Adipose/Fat
Porcine

Porcine, 2-6 year

Mitić, R. , Cantoni, F. , Börlin, C. , Post, M. and Jackisch, L.
A Simplified and Defined Serum-free Medium for Cultivating Fat Across Species. , iScience Vol. 26 , 105822 , 2023
Cells
Stomal vascular cells
Medium
Growth medium
Adipose/Fat
Porcine
Porcine, female, <1 year
Williams, K. , Picou, A. , Kish, S. , Giraldo, A. , Godke, R. and Bondioli, K
Isolation and Characterization of Porcine Adipose Tissue-Derived Adult Stem Cells. ,
Cells Tissues Organs
 188 , 251 , 2008
Cells
Adipose mesenchymal stem
Enzymes
Medium
DMEM
Adipose/Fat
Porcine
Porcine, crossbred, male 1-4 day
Ramsay, T.G.
Porcine Leptin Inhibits Lipogenesis in Porcine Adipocytes ,
J Anim Sci
 81 , 3008 , 2003
Cells
Adipocytes
Enzymes
Medium
DMEM/F12
Adipose/Fat
Porcine
Porcine, crossbred
Liang, W. and Mills, S.
Quantitative Analysis of beta-Adrenergic Receptor Subtypes in Pig Tissues. ,
J Anim Sci
 80 , 963-70 , 2002
Cells
Adipocytes
Enzymes
Medium
Krebs-Ringer
Adipose/Fat
Porcine
Porcine, neonatal
Wang Y, Fried SK, Petersen RN, Schoknecht PA.
Somatotropin regulates adipose tissue metabolism in neonatal swine ,
J Nutr
 129 , 139-45 , 1999
Cells
Adipocytes
Enzymes
Medium
Krebs-Ringer bicarbonate albumin
Adipose/Fat
Porcine
Porcine, 8-9 week
Ding, S. , McNeel, R. and Mersmann, H.
Expression of Porcine Adipocyte Transcripts: Tissue Distribution and Differentiation In Vitro and In Vivo ,
Comp Biochem Physiol B
 123 , 307 , 1999
Cells
Adipose
Enzymes
Collagenase Type 1 : 300 u/ml
Medium
HEPES
Adipose/Fat
Porcine
Porcine, crossbred, 1-3 day
Suryawan, A. , Swanson, L. and Hu, C.
Insulin and Hydrocortisone, But Not Triiodothyronine, Are Required for the Differentiation of Pig Preadipocytes in Primary Culture ,
J Anim Sci
 75 , 105 , 1997
Cells
Adipose, Stromal-vascular
Enzymes
Medium
DMEM/F12
Brain
Insect
Drosophilia
Hadzic, T. , Park, D. , Abruzzi, K. , Yang, L. , Trigg, J. , Rohs, R. , Rosbash, M. and Taghert, P.
Genome-Wide Features of Neuroendocrine Regulation in Drosophila by the Basic Helix-Loop-Helix Transcription Factor DIMMED. ,
Nucleic Acids Res
 43 , 2199-215 , 2015
Cells
Neurons
Enzymes
Papain : 50 u/ml
Medium
Modified Dissecting Saline
Brain
Insect
Drosophila
Gu, H. and O'Dowd, D.
Whole Cell Recordings from Brain of Adult Drosophila. ,
J Vis Exp
 6 , 248 , 2007
Cells
Neurons
Enzymes
Papain : 20 u/ml
Medium
Saline
Brain
Insect
Drosophila
Sicaeros, B. Campusano, J. and O'Dowd, D.
Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae. ,
J Vis Exp
 4 , 200 , 2007
Cells
Neurons
Enzymes
Papain : 4 u/ml
Medium
DMEM
Brain
Insect
Drosophilia
Gu, H. and O'Dowd, D.
Cholinergic Synaptic Transmission in Adult Drosophila Kenyon Cells in Situ. ,
J Neurosci
 26 , 265-72 , 2006
Cells
Kenyon
Enzymes
Papain : 20 u/ml
Medium
Recording saline
Brain
Porcine
Mini pigs, Yucatan (Susscrofa Yucatan), 4-6 months
Robinson, D.H. , Kang,Y. , Deschner, S.H. and Nielsen, T.B.
Morphologic Plasticity and Periodicity : Porcine Cerebral Microvascular Cells in Culture ,
In Vitro Cell Dev Biol
 26 , 169 , 1990
Cells
Microvascular
Enzymes
Collagenase : 0.1%
Medium
HBSS
Cartilage
Equine
Equine
Visser, J. , Levett, P. , te Moller, N. , Besems, J. , Boere, K. , van Rijen, M. , de Grauw, J. , Dhert, W. , van Weeren, P. and Malda, J.
Crosslinkable Hydrogels Derived from Cartilage, Meniscus, and Tendon Tissue. ,
Tissue Eng Part A
 21 , 1195-206 , 2015
Cells
Chondrocytes
Enzymes
Medium
DMEM
Cartilage
Equine
Equine
Ley, C. , Svala, E. , Nilton, A. , Lindahl, A. , Eloranta, M. , Ekman, S. and Skioldebrand, E.
Effects of High Mobility Group Box Protein-1, Interleukin-1B, and Interleukin-6 on Cartilage Matrix Metabolism in Three-Dimensional Equine Chondrocyte Cultures. ,
Conn Tissue Res
 52 , 290-300 , 2011
Cells
Chondrocytes
Enzymes
Medium
DMEM/F12
Cartilage
Porcine
Porcine, 1 year
Chowdhury, T. , Schulz, R. , Rai, S. , Thuemmler, C. , Wuestneck, N. , Bader, A. and Homandberg, G
Biomechanical Modulation of Collagen Fragment-Induced Anabolic and Catabolic Activities in Chondrocyte/Agarose Constructs. ,
Arthritis Res Ther
 12 , R82 , 2010
Cells
Chondrocytes
Enzymes
Medium
DEMEM
Cartilage
Porcine
Porcine, 2-4 month
Graff RD, Lazarowski ER, Banes AJ, Lee GM.
ATP release by mechanically loaded porcine chondrons in pellet culture ,
Arthritis Rheum
 43 , 1571-9 , 2000
Cells
Chondrons
Enzymes
Medium
PBS
Endothelial
Porcine
Porcine, 6 month
Balaoing, L. , Post, A. , Lin, A. , Tseng, H. , Moake, J. and Grande-Allen, K.
Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation. ,
PLoS ONE
 10 , e0130749 , 2015
Cells
Valvular endothelial
Enzymes
Medium
PBS
Endothelial
Porcine
Porcine
Gould, R. and Butcher, J.
Isolation of Valvular Endothelial Cells. ,
J Vis Exp
 46 , e2158 , 2010
Cells
Valvular endothelial
Enzymes
Collagenase Type 2 : 300-600 u/ml
Medium
DMEM
Endothelial
Porcine
Porcine
(also bovine)
Nugent, H. and Edelman, E.
Endothelial Implants Provide Long-Term Control of Vascular Repair in a Porcine Model of Arterial Injury ,
J Surg Res
 99 , 228 , 2001
Cells
Endothelial
Medium
DMEM w/ 10% calf serum
Endothelial
Porcine
Porcine
Kwak HJ, Lee SJ, Lee YH, Ryu CH, Koh KN, Choi HY. and Koh GY.
Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells ,
Circulation
 101(19) , 2317 , 2000
Cells
HUVEC, porcine pulmonary arterial endothelial cells
Enzymes
Medium
DMEM
Endothelial
Porcine
Porcine, 6-7 month
Hill-Kapturczak N, Kapturczak MH, Block ER, Patel JM, Malinski T, Madsen KM. and Tisher CC.
Angiotensin II-stimulated nitric oxide release from porcine pulmonary endothelium is mediated by angiotensin IV ,
J Am Soc Nephrol
 10(3) , 481 , 1999
Cells
Porcine pulmonary endothelial
Enzymes
Medium
RPMI 1640
Endothelial
Porcine
Porcine
Shasby, S.
Endothelial Cells Grown On Permeable Membrane Supports ,
J Tiss Cul Meth
 14 , 247 , 1992
Cells
Endothelial
Enzymes
Trypsin : 0.25%
Medium
Medium 199
Endothelial
Porcine
Porcine
Vischer, P. and Buddecke, E.
Alteration of Glycosytransferase Activities during Proliferation of Cultivated Arterial Endothelial Cells and Smooth Muscle Cells ,
Exp Cell Res
 158 , 15 , 1985
Cells
Endothelial
Enzymes
Trypsin : 0.05%
Medium
DMEM
Endothelial
Porcine
Porcine
Dickinson, E. and Slakey, L.
Plasma-derived Serum as a Selective Agent to Obtain Endothelial Cultures from Swine Aorta ,
In Vitro
 18 , 63 , 1982
Cells
Endothelial
Aortic
Enzymes
Medium
Dulbecco-Vogt MEM w/o serum
Endothelial
Porcine
Porcine, 30-40 kg
Merrilees, M.J. and Scott, L.
Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture ,
Atherosclerosis
 39 , 147 , 1981
Cells
Endothelial
Thoracic aorta
Enzymes
Trypsin : 0.05%
Medium
Medium 199 and 0.01M EDTA
Endothelial
Porcine
Porcine, 20-30 week
Slater, D.N. and Sloan, J.M.
The Porcine Endothelial Cell in Tissue Culture ,
Atherosclerosis
 21 , 259 , 1975
Cells
Endothelial
Aortas
Veins
Enzymes
Collagenase : 0.1%
Medium
Medium 199 w/BSS and HEPES or NaHCO3
Endothelial
Porcine
Porcine, 60-100 days
Coulson, W.F.
The Effect Of Proteolytic Enzymes on the Tensile Strength of Whole Aorta and Isolated Aortic Elastin ,
Biochim Biophys Acta
 237 , 378 , 1971
Cells
Aorta
Enzymes
Trypsin : 0.1%
Medium
Phosphate buffer
(see reference)
Epithelial
Porcine
Porcine, 5-60 kg
Wiencke, A. , Kiilgaard, J. , Nicolini, J. , Bundgaard, M. , Ropke, C. and La Cour, M.
Growth of Cultured Porcine Retinal Pigment Epithelial Cells ,
Acta Opthalmol Scand
 81(2) , 170 , 2003
Cells
Retinal pigment epithelial cells
Enzymes
Medium
DMEM
Epithelial
Porcine
Porcine
De Buysscher, E. , Kennedy, J. and Mendicino, J.
Synthesis of Mucin Glycoproteins by Epithelial Cells Isolated from Swine Trachea by Specific Proteolysis ,
In Vitro
 20 , 433 , 1984
Cells
Trachea
Enzymes
Medium
HBSS
Eye
Porcine
Porcine, adult
Xu, N. , Chen, Y. , Dean, K. , Lu, X. , Liu, X. , Wang, W. , Dean, D. , Kaplan, H. , Gao, L. , Dong, F. and Liu, Y.
Sphere-Induced Rejuvenation of Swine and Human Muller Glia Is Primarily Caused by Telomere Elongation. ,
Stem Cells
 35 , 1579-1591 , 2017
Cells
Muller glia
Enzymes
PDS kit : per instructions
Medium
DMEM
Eye
Porcine
Porcine, 5-60 kg
Wiencke, A. , Kiilgaard, J. , Nicolini, J. , Bundgaard, M. , Ropke, C. and La Cour, M.
Growth of Cultured Porcine Retinal Pigment Epithelial Cells ,
Acta Opthalmol Scand
 81(2) , 170 , 2003
Cells
Retinal pigment epithelial cells
Enzymes
Medium
DMEM
Heart
Porcine
Porcine
Butcher, J. and Nerem, R.
Porcine Aortic Valve Interstitial Cells in Three-Dimensional Culture: Comparison of Phenotype with Aortic Smooth Muscle Cells. ,
J Heart Valve Dis
 13 , 478 , 2004
Cells
Aortic smooth muscle and intersitial cells
Enzymes
Collagenase : 600 u/ml
Medium
PBS
Heart
Porcine
Porcine
Christ, M. , Gunther, A. , Heck, M. , Schmidt, B. , Falkenstein, E. and Wehling, M.
Aldosterone, Not Estradiol, Is the Physiological Agonist for Rapid Increases in cAMP in Vascular Smooth Muscle Cells ,
Circulation
 99 , 1485 , 1999
Cells
Coronary vascular smooth muscle (PCVSMCs)
Enzymes
Trypsin : 0.037%
Medium
HEPES
Heart
Porcine
Miniature swine, adult, female, 25-40 kg, familiarized with treadmill exercise
Bowles, D. , Hu, Q. , Laughlin, M. and Sturek, M.
Exercise Training Increases L-type Calcium Current Density in Coronary Smooth Muscle ,
Am J Physiol
 275 (44) , H2159 , 1998
Cells
Coronary smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Krebs bicarbonate solution

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.