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Liver
Porcine
Porcine, adult
Zhou X, Liu L, Kano J, Mukaiyama T. and Tokiwa T.
Isolation and Cultivation of Porcine Hepatocytes for Extracorporeal Artificial Liver Support System ,
Chin Med J
 114 , 946 , 2001
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
MEM
Liver
Porcine
Porcine, male, 6-40 kg
Gerlach, J. , Brombacher, J. , Smith, M. and Neuhaus, P.
High Yield Hepatocyte Isolation from Pig Livers for Investigation of Hybrid Liver Support Systems: Influence of Collagenase Concentration and Body Weight ,
J Surg Res
 62 (1) , 85 , 1996
Cells
Hepatocytes
Enzymes
Collagenase : 0.8%
Medium
PBS
Lung
Porcine
Swine fetuses (70 day), adult Amer Dutch 250 day
(also human fetuses, 80 day)
Hinz, R. and Syverton, J.
Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase ,
Proc Soc Exp Biol Med
 101 , 19 , 1959
Cells
Lung
Enzymes
Collagenase : 0.01%
Medium
HBSS
Miscellaneous
Invertebrate

Octopus vulgaris

Maselli, V. , Xu, F. , Syed, N. , Polese, G. and Cosmo, A.
A Novel Approach to Primary Cell Culture for Octopus vulgaris Neurons. ,
Front Physiol
 9 , 220 , 2018
Cells
Neurons
Enzymes
Papain : 0.1%
Trypsin : 0.1%
Medium
L15-ASW
Miscellaneous
Invertebrate
Squid
Raman, M. and Mathew, S.
Study of Chemical Properties and Evaluation of Collagen in Mantle, Epidermal Connective Tissue and Tentacle of Indian Squid, Loligo duvauceli Orbigny. ,
J Food Sci Technol
 51 , 1509-16 , 2014
Cells
Mantle
Enzymes
Collagenase : see reference
Papain : see reference
Medium
Phosphate buffer
Miscellaneous
Porcine
Porcine, 6 month
Kean, T. and Dennis, J.
Synoviocyte Derived-Extracellular Matrix Enhances Human Articular Chondrocyte Proliferation and Maintains Re-Differentiation Capacity at Both Low and Atmospheric Oxygen Tensions. ,
PLoS ONE
 10 , e0129961 , 2015
Cells
Synoviocytes
Enzymes
Hyaluronidase : 660 u/ml
Trypsin : 0.25%
Collagenase Type 2 : 583 u/ml
Medium
DMEM
Miscellaneous
Porcine
Porcine, <10 week
Potier, E. and Ito. K.
Can Notochordal Cells Promote Bone Marrow Stromal Cell Potential for Nucleus Pulposus Enrichment? A Simplified in Vitro System. ,
Tissue Eng Part A
 20 , 3241-51 , 2014
Cells
Nucleus pulposus
Enzymes
Collagenase : 0.05%
Pronase : 0.3%
Medium
DMEM
Miscellaneous
Porcine
Porcine, various ages
Cho, H. , Park, S. , Lee, S. , Kang, M. , Hasty, K. and Kim, S.
Snapshot of Degenerative Aging of Porcine Intervertebral Disc: A Model to Unravel the Molecular Mechanisms. ,
Exp Mol Med
 43 , 334-40 , 2011
Cells
Nucleous pulposus and AF
Enzymes
Pronase : 0.05%
Collagenase : 0.2%
Medium
Modified F-12K
Muscle
Porcine
Porcine, postnatal
Miersch, C. , Stange, K. and Rontgen, M.
Effects of Trypsinization and of a Combined Trypsin, Collagenase, and DNase Digestion on Liberation and in Vitro Function of Satellite Cells Isolated from Juvenile Porcine Muscles. ,
In Vitro Cell Dev Biol Anim
 54 , 406-412 , 2018
Cells
Muscle satellite
Enzymes
Medium
Eagle MEM
Muscle
Porcine
Porcine, 2 month
Lewis, F. , Henning, B. , Marazzi, G. , Sassoon, D. , Ellison, G. and Nadal-Ginard, B.
Porcine Skeletal Muscle-Derived Multipotent PW1pos/Pax7neg Interstitial Cells: Isolation, Characterization, and Long-Term Culture. ,
Stem Cells Transl Med
 3 , 702-12 , 2014
Cells
Skeletal muscle
Enzymes
Medium
HBSS
Muscle
Porcine
Porcine, male, 25-40 kg
Korzick, D. , Laughlin, M. and Bowles, D.
Alterations in PKC signaling underlie enhanced myogenic tone in exercise-trained porcine coronary resistance arteries ,
J Appl Physiol
 96 , 1425-32 , 2004
Cells
Coronary myocytes
Enzymes
Medium
low calcium physiological saline
Muscle
Porcine
Porcine, adult, 35-45 kg
Wamhoff BR, Dixon JL, Sturek M.
Atorvastatin treatment prevents alterations in coronary smooth muscle nuclear Ca2+ signaling in diabetic dyslipidemia ,
J Vasc Res
 39 , 208 , 2002
Cells
Arterial smooth muscle
Enzymes
Collagenase Type 2 : 294 u/ml
Elastase : 6.5 u/ml
Deoxyribonuclease I : 0.4 mg/ml
Medium
MEM
Muscle
Porcine
Porcine, Yorkshire, 30kg
Sirous ZN, Fleming JB, Khalil RA.
Endothelin-1 enhances eicosanoids-induced coronary smooth muscle contraction by activating specific protein kinase C isoforms ,
Hypertension
 37 , 497-504 , 2001
Cells
Coronary smooth muscle
Enzymes
Collagenase Type 2 : 150 u/ml
Elastase : 0.05%
Medium
HBSS
Muscle
Porcine
Porcine
Shieh CC, Feng J, Buckner SA, Brioni JD, Coghlan MJ, Sullivan JP, Gopalakrishnan M.
Functional implication of spare ATP-sensitive K(+) channels in bladder smooth muscle cells ,
J Pharmacol Exp Ther
 296 , 669-75 , 2001
Cells
Bladder smooth muscle
Enzymes
Collagenase Type 2 : 0.1-0.2%
Medium
Krebs-Ringer bicarbonate
Muscle
Porcine
Porcine, juvenile, 30kg
Huckle WR, Drag MD, Acker WR, Powers M, McFall RC, Holder DJ, Fujita T, Stabilito II, Kim D, Ondeyka DL, Mantlo NB, Chang RS, Reilly CF, Schwartz RS, Greenlee WJ, Johnson RG.
Effects of subtype-selective and balanced angiotensin II receptor antagonists in a porcine coronary artery model of vascular restenosis ,
Circulation
 93 , 1009-19 , 1996
Cells
Coronary smooth muscle cells
Enzymes
Collagenase : 0.3%
Elastase : 0.05%
Medium
HBSS
Muscle
Porcine
Porcine
Xiong, Yimin, Xu, Shangzhe. and Slakey, Linda L
Modulation of Response to Adenosine in Vascular Smooth Muscle Cells Cultured in Defined Medium ,
In Vitro Cell Dev Biol
 27 , 355 , 1991
Cells
Smooth muscle, aorta
Enzymes
Collagenase : 0.3%
Medium
DMEM
Muscle
Porcine
Porcine
Breton, M. , Berrou, E. , Deudon, E. and Picard, J.
Changes in Proteoglycans of Cultured Pig Aortic Smooth Muscle Cells During Subculture ,
In Vitro Cell Dev Biol
 26 , 157 , 1990
Cells
Smooth muscle
Aorta
Enzymes
Trypsin : 0.05%
Medium
EDTA 0.02%
Muscle
Porcine
Porcine
Fehr, T. , Dickinson, E. , Goldman, S. and Slakey, L.
Cyclic AMP Efflux is Regulated by Occupancy of The Adenosine Receptor in Pig Aortic Smooth Muscle Cells ,
J Biol Chem
 265 , 10974 , 1990
Cells
Smooth muscle
Aortic medial tissue
Enzymes
Collagenase : 0.30%
Medium
DMEM
Neural
Porcine
Porcine, adult, 60-100 kg
Si ML, Lee TJ.
Presynaptic alpha7-nicotinic acetylcholine receptors mediate nicotine-induced nitric oxidergic neurogenic vasodilation in porcine basilar arteries ,
J Pharmacol Exp Ther
 298 , 122 , 2001
Cells
Superior cervical ganglia
Enzymes
Papain : 2 u/ml
Collagenase : 0.12%
Medium
HBSS
Pancreas
Porcine
Porcine, juvenile
Van der Burg Michael P M, Graham John M.
Iodixanol Density Gradient Preparation in University of Wisconsin solution for porcine islet purification ,
ScientificWorldJournal
 3 , 1154-9 , 2003
Cells
Islets
Enzymes
Collagenase : see reference
Medium
Univ of Wisconsin solution
Pancreas
Porcine
Porcine, 3 month, 15-20 kg
Zhao, X. , Han, J. and Tang, C.
Primary Culture of Porcine Pancreatic Acinar Cells ,
JOP
 2 (2) , 78 , 2001
Cells
Acinar
Enzymes
Collagenase Type 3 : 200 u/ml
Medium
RPMI 1640
Pancreas
Porcine
Porcine, 1-3 year, 1.5-2.0 kg, either sex
Korbutt, G. , Elliott, J. , Ao, Z. , Smith, D. , Warnock, G. and Rajotte, R.
Large Scale Isolation, Growth, and Function of Porcine Neonatal Islet Cells ,
J Clin Invest
 97 (9) , 2119 , 1996
Cells
Islets
Enzymes
Collagenase : 0.25%
Medium
HBSS
Pancreas
Porcine
Porcine, female
Brandhorst, D. , Brandhorst, H. , Hering, B. , Federlin, K. and Bretzel, R.
Islet Isolation from the Pancreas of Large Mammals and Humans: 10 Years of Experience ,
Exp Clin Endocrinol
 103 , 3 , 1995
Cells
Islets
Enzymes
Collagenase : 0.1%
Medium
HBSS
Pancreas
Porcine
Porcine
Johnson, P. , van Suylichem, P. , Roberts, D. , Vos-Scheperkeuter, G. , White, S. , van Schlifgaarde, R. and London, N.
Design of a Simple, in vitro Method for Evaluation of the Efficiency of Crude Clostridium histolyticum Collagenase and its Components for Porcine Islet Isolation ,
Xenotransplantation
 2 , 165 , 1995
Cells
Islets
Enzymes
Collagenase : 0.1%-0.2%
Medium
HBSS
Pancreas
Porcine
Porcine, <2 year, 200-250 kg
Heiser, A. , Ulrichs, K. and Muller-Ruchholtz, W.
Isolation of Porcine Pancreatic Islets: Low Trypsin Activity During the Isolation Procedure Guarantees Reproducible High Islet Yields ,
J Clin Lab Anal
 8 , 407 , 1994
Cells
Islets
Enzymes
Collagenase : 0.1%
Medium
HBSS
Pancreas
Porcine
Porcine, 10-36 months, 200-300 kg
Ricordi, C. , Socci, C. , Davalli, A. , Staudacher, C. , Baro, P. , Vertova, A. , Sassi, I. , Gavazzi, F. , Pozza, G. and Di Carlo, V.
Isolation of the Elusive Pig Islet ,
Surgery
 107 (6) , 688 , 1990
Cells
Islets
Enzymes
Collagenase : 0.2%
Medium
HBSS
Pancreas
Porcine
Porcine
Iwatsuki, N. and Peterson, O.
Action of Tetraethylammonium on Calcium-Activated Potassim Channels in Pig Pancreatic Acinar Cells Studied by Patch-Clamp Single-Channel and Whole-Cell Current Recording ,
J Membr Biol
 86 , 139 , 1985
Cells
Acinar
Enzymes
Collagenase : 100 u/ml
Medium
Saline
Reproductive
Porcine
Porcine, 1-4 day
Park, M. , Park, J. , Kim, M. , Lee, K. , Park, H. , Yun, J. , Choi, J. , Lee, E. and Lee, S.
Development of a High-Yield Technique to Isolate Spermatogonial Stem Cells From Porcine Testes. ,
J Assist Reprod Genet
 31 , 983-91 , 2014
Cells
Testicular
Enzymes
Hyaluronidase : 0.1%
Trypsin : 0.25%
Medium
DMEM
Reproductive
Porcine
Porcine, male, 8 day
Dirami G, Ravindranath N, Pursel V, Dym M.
Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM ,
Biol Reprod
 61 , 225-30 , 1999
Cells
Seminiferous epithelial cells
Enzymes
Collagenase : 0.15%
Hyaluronidase : 0.15%
Trypsin : 0.05%
Medium
DMEM/F12
Reproductive
Porcine
Porcine, female
Ciereszko, R. , Petroff, B. , Ottobre, A. , Guan, Z. , Stokes, B. and Ottobre, J.
Assessment of the Mechanism by Which Prolactin Stimulates Progesterone Production by Early Corpora Lutea of Pigs ,
J Endocrinol
 159 , 201 , 1998
Cells
Corpus Leuteum
Enzymes
Collagenase Type 4 : 600 u/ml
Medium
Medium 199
Reproductive
Porcine
Porcine (also bovine, human)
Marcus, G. , Connor, L. , Domingo, M. , Tsang, B. , Downey, B. and Ainsworth, L.
Enzymatic Dissociation of Ovarian and Uterine Tissues ,
Endocr Res
 10 , 151 , 1984
Cells
Interna & corpus luteum
Endometrium
Ovarian
Uterine
Enzymes
Pronase : 0.1%
Medium
Moscona's BSS
Reproductive
Porcine
Porcine, 3-4 week
Mather, J. , Saez, J. and Haour, F.
Regulaton of Gonadotropin Receptors and Steroidogenesis in Cultured Porcine Leydig Cells ,
Endocrinology
 110 , 933 , 1982
Cells
Leydig
Testis
Enzymes
Trypsin : 0.0003%
Medium
Lebovitz L-15 Medium
Skin
Porcine
Porcine, 3-4 month
Ando, W. , Kutcher, J. , Krawetz, R. , Sen, A. , Nakamura, N. , Frank, C. and Hart, D.
Clonal Analysis of Synovial Fluid Stem Cells to Characterize and Identify Stable Mesenchymal Stromal Cell/Mesenchymal Progenitor Cell Phenotypes in a Porcine Model: A Cell Source with Enhanced Commitment to the Chondrogenic Lineage. ,
Cytotherapy
 16 , 776 , 2014
Cells
Synovial membrane and skin stem
Enzymes
Collagenase : 0.2%
Medium
DMEM
Skin
Porcine
Porcine, 2-6 month
Regauer S, Compton C.
Cultured porcine epithelial grafts: an improved method ,
J Invest Dermatol
 94 , 230-4 , 1990
Cells
Keratinocytes
Enzymes
Medium
Dulbecco-Vogt MEM
Stem
Porcine
Porcine, 3-4 month
Ando, W. , Kutcher, J. , Krawetz, R. , Sen, A. , Nakamura, N. , Frank, C. and Hart, D.
Clonal Analysis of Synovial Fluid Stem Cells to Characterize and Identify Stable Mesenchymal Stromal Cell/Mesenchymal Progenitor Cell Phenotypes in a Porcine Model: A Cell Source with Enhanced Commitment to the Chondrogenic Lineage. ,
Cytotherapy
 16 , 776 , 2014
Cells
Synovial membrane and skin stem
Enzymes
Collagenase : 0.2%
Medium
DMEM
Stem
Porcine
Porcine, 1-4 day
Park, M. , Park, J. , Kim, M. , Lee, K. , Park, H. , Yun, J. , Choi, J. , Lee, E. and Lee, S.
Development of a High-Yield Technique to Isolate Spermatogonial Stem Cells From Porcine Testes. ,
J Assist Reprod Genet
 31 , 983-91 , 2014
Cells
Testicular
Enzymes
Hyaluronidase : 0.1%
Trypsin : 0.25%
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.