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Tissue References Search Tool

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Neural
Rat
Rat, pups, 24-48 h old
Hall, R. and Soderling, T.
Differential Surface Expression and Phosphorylation of the N-Methyl-D-Aspartate Receptor NR1 and NR2 in Cultured Hippocampal Neurons ,
J Biol Chem
 272 (7) , 4135 , 1997
Cells
Neurons, hippocampal
Enzymes
Papain : 20 u/ml
Medium
Harvest buffer
Neural
Rat
Rat, SD, Fisher
Brewer, G.J.
Isolation and culture of adult rat hippocampal neurons of any age ,
J Neurosci Methods
 71 , 143 , 1997
Cells
Hippocampal neurons
Enzymes
Papain : 0.2%
Medium
HibernateA/B27
Neural
Rat
Rat, SD, adult, 250-300g, P8
Davies, S. , Fitch, M. , Memberg, S. , Hall, A, Raisman, G. and Silver, J.
Regeneration of Adult Axons in White Matter Tracts of the Central Nervous System ,
Nature
 390 , 680 , 1997
Cells
Neurons, DRG
Enzymes
Medium
L-15 w/ CO2
Neural
Rat
Rat, 1-4 day old
Twitchell, W. , Brown, S. and Mackie, K.
Cannabinoids Inhibit N- and P/Q-Type Calcium Channels in Cultured Rat Hippocampal Neurons ,
J Neurophysiol
 78 , 43 , 1997
Cells
Neurons, hippocampal
Enzymes
Papain : 20 u/ml
Medium
MEM
Neural
Rat
Rat, E18
Liu QY, Schaffner AE, Li YX, Dunlap V, Barker JL.
Upregulation of GABAA current by astrocytes in cultured embryonic rat hippocampal neurons ,
J Neurosci
 16 , 2912-23 , 1996
Cells
Hippocampal neurons
Enzymes
Papain : 20 u/ml
Medium
MEM
Neural
Rat
Rat, 1-6 day
Robertson, S. , Rae, M. , Rowan, E. and Kennedy, C.
Characterization of a P2X-Purinoceptor in Cultured Neurones of the Rat Dorsal Root Ganglia. ,
Br J Pharmacol
 118 , 951 , 1996
Cells
DRG neurons
Enzymes
Papain : 20 u/ml
Collagenase Type 1 : 150 u/ml
Medium
HBSS
Neural
Rat
Rat, pups, 14 day old
McFarlane, S. and Cooper, E.
Extrinsic Factors Influence the Expression of Voltage-Gated K Currents on Neonatal Rat Sympathetic Neurons ,
J Neurosci
 13 (6) , 2591 , 1993
Cells
Neurons, sympathetic
Enzymes
Medium
HBSS
Neural
Rat
Rats, SD, 11-14 day old, 29-32 g
Allen, T. , Sim, J. and Brown, D.
The Whole-Cell Calcium Current in Acutely Dissociated Magnocellular Cholinergic Basal Forebrain Neurones of the Rat ,
J Physiol
 460 , 91 , 1993
Cells
Basal forebrain neurons
Enzymes
Trypsin : 0.125%
Medium
Gey's BSS
Neural
Rat
Rat, Wistar-Hanover, 7-12 day
Rayport, S. , Sulzer, D. , Shi, W. , Sawasdikosol, S. , Monaco, J. , Batson, D. and Rajendran, G.
Identified Postnatal Mesolimbic Dopamine Neurons in Culture: Morphology and Electrophysiology ,
J Neurosci
 12 (11) , 4264 , 1992
Cells
Postnatal dopamine neurons
Enzymes
Trypsin : 0.035%
Medium
(see reference)
Neural
Rat
Rat
Stemple, D. and Anderson, D.
Isolation of a Stem Cell for Neurons and Glia from the Mammalian Neural Crest ,
Cell
 71 , 973 , 1992
Cells
Stem, neural crest
Enzymes
Collagenase : 0.075%
Medium
Ringer's solution
Neural
Rat
Rat, SD, 18-day-old, fetus
Cheng, B. and Mattson, M.
IGF-I and IGF-II Protect Cultured Hippocampal and Septal Neurons Against Calcium-Mediated Hypoglycemic Damage ,
J Neurosci
 12 (4) , 1558 , 1992
Cells
Hippocampal
Enzymes
Trypsin : 0.2%
Medium
HBSS
Neural
Rat
Rat, SD, pregnant, Charles River
Buchhalter, J. and Dichter, M.
Electrophysiological Comparison of Pyramidal and Stellate Nonpyramidal Neurons in Dissociated Cell Culture of Rat Hippocampus ,
Brain Res Bull
 26 , 333 , 1991
Cells
Pyramidal neurons
Nonpyramidal neurons
Enzymes
Trypsin : 0.027%
Medium
HEPES
Neural
Rat
Rat, SD, embryos, 19-21 days gestation
Mithen, F. , Reiker, M. and Birchem, R.
Effects of Ethanol on Rat Schwann Cell Proliferation and Myelination in Culture ,
In Vitro Cell Dev Biol
 26 , 129 , 1990
Cells
Schwann, dorsal root ganglia
Enzymes
Trypsin : 0.25%
Medium
HBSS, CMF
Neural
Rat
Rat
(also bovine)
Trifaro, J. , Tang, R. and Novas, M.
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
 26 , 335 , 1990
Cells
Heart
Adrenal chromaffin
Paraneurons
Enzymes
Trypsin : 0.06%
Medium
25mM HEPES buffered Locke's solution, CMF
Neural
Rat
Rat, SD, 8 day old pups
Novelli, A. , Reilly, J. , Lysko, P. and Henneberry, R.
Glutamate Becomes Neurotoxic Via the N-methyl-D-aspartate Receptor When Intracellular Energy Levels are Reduced ,
Brain Res
 451 , 205 , 1988
Cells
Cerebellar neurons
Enzymes
Trypsin : 0.025%
Medium
Eagle's MEM
Neural
Rat
Rat, fetuses, 18 day old
Mattson, M. and Kater, S.
Isolated Hippocampal Neurons in Cryopreserved Long-Term Cultures: Development of Neuroarchitecture and Sensitivity to Nmda ,
Int J Dev Neurosci
 6 (5) , 439 , 1988
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.2%
Medium
Eagle's MEM
Neural
Rat
Rat, postnatal
Hatanaka,H. , Tsukui, H. and Nihonmatsu, I.
Septal Cholinergic Neurons From Postnatal Rat Can Survive In The Dissociate Culture Conditions In The Presence Of Nerve Growth Factor ,
Neurosci Lett
 79 , 85 , 1987
Cells
Septal neurons
Enzymes
Papain : 0.05%
Medium
PBS, CMF
Neural
Rat
Rat, SD, female, 4 month old
Wood, P. and Bunge, R.
Evidence That Axons are Mitogenic for Oligodendrocytes Isolated From Adult Animals ,
Nature
 320 , 756 , 1986
Cells
CNS cells
Enzymes
Trypsin : 0.25%
Medium
EBSS
Neural
Rat
Rat, Long Evans, 1-15 days
Huettner, J. and Baughman, R.
Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats ,
J Neurosci
 6 , 3044 , 1986
Cells
Neurons, visual cortex
Enzymes
Papain : 20 u/ml
Medium
BSS
(see reference)
Neural
Rat
Rat, embryo, 15 day
Jahr, C. and Jessell, T.
Synaptic Transmission between Dorsal Root Ganglion and Dorsal Horn Neurons in Culture: Antagonism of Monosynaptic Excitatory Postsynaptic Potentials and Glutamate Excitation by Kynurenate ,
J Neurosci
 5 , 2281 , 1985
Cells
Dorsal horn neurons
Spinal
Enzymes
Trypsin : 0.025%
Medium
Ham's F-12
Neural
Rat
Rat, postnatal
Leifer, D. , Lipton, S. , Barnstable, C. and Masland, R.
Monoclonal Antibody to Thy-1 Enhances Regeneration of Processes by Rat Retinal Ganglion Cells in Culture ,
Science
 224 , 303 , 1984
Cells
Ganglion, retina
Enzymes
Papain : 12.5 u/ml
Medium
HBSS w/5 mM HEPES
Neural
Rat
Rat, fetus, 18-20 day
Bartlett, W. and Banker, G.
An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts ,
J Neurosci
 4 , 1944 , 1984
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.25%
Medium
HBSS, CMF
Neural
Rat
Rat, SD, pups
Sarthy PV, Curtis BM. and Catterall WA.
Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat ,
J Neurosci
 3 (12) , 2532 , 1983
Cells
Retina
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Neural
Rat
Rat, W/FU, 5-8 day
Raff, M. , Fields, K. , Hakomori, S. , Mirsky, R. , Pruss, R. and Winter, J.
Cell-Type-Specific Markers for Distinguishing and Studying Neurons And the Major Classes of Glial Cells in Culture ,
Brain Res
 174 , 283 , 1979
Cells
Neurons and glial
Enzymes
Trypsin : 0.25%
Medium
MEM
(see reference)
Neural
Rat
Rat, fetus
Wakshull, E. , Johnson, M. and Burton, H.
Postnatal Rat Sympathetic Neurons In Culture. 1. A Comparison With Embryonic Neurons ,
J Neurophysiol
 42 , 1410 , 1979
Cells
Neurons, sympathetic
Enzymes
Trypsin : 0.25%
Medium
L-15 or HBSS, CMF
Neural
Rat
Rat, Wistar/Furth, newborn
(also bovine)
Brockes, J. , Fields, K. and Raff, M.
Studies on Cultured Rat Schwann Cells. I. Establishment of Purified Populations From Cultures of Peripheral Nerve ,
Brain Res
 165 , 105 , 1979
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
Neural
Rat
Rat, embryo
Dichter, M.
Rat Cortical Neurons in Cell Culture: Culture Methods, Cell Morphology, Electrophysiology, and Synapse Formation ,
Brain Res
 149 , 279 , 1978
Cells
Neurons, cortical
Enzymes
Trypsin : 0.027%
Medium
MEM
Neural
Rat
Rat, newborn
Reichardt, L. and Patterson, P.
Neurotransmitter Synthesis and Uptake by Isolated Sympathetic Neurones in Microcultures ,
Nature
 270 , 147 , 1977
Cells
Neurons, sympathetic
Enzymes
Medium
Hank's solution, CF
Neural
Rat
Rat, neonatal
Mains, R. and Patterson, P.
Primary Cultures of Dissociated Sympathetic Neurons I. Establishment of Long-Term Growth in Culture and Studies of Differentiated Properties ,
J Cell Biol
 59 , 329 , 1973
Cells
Neurons, superior cervical ganglia
Enzymes
Trypsin : 0.1%
Medium
Basal L-15 medium
Pancreas
Rabbit
Rabbit, New Zealand white, Male/Female, 2-3 Kg
Renckens, B. , Schrijen, J. , Swarts, H. , DePont, J. and Bonting, S.
Role of Calcium in Exocrine Pancreatic Secretion. IV. Calcium Movements in Isolated Acinar Cells of Rabbit Pancreas ,
Biochim Biophys Acta
 544 , 338 , 1978
Cells
Acinar
Enzymes
Hyaluronidase : 0.2%
Medium
Kreb's Ringer bicarbonate buffer
Pancreas
Rabbit
Rabbit, also hamster
Feldman, J. and Chapman, B.
Preparation of Islets of Langerhans from Rabbits and Hamsters by the Collagenase Digestion Technique ,
Acta Diabetol
 12 , 208 , 1975
Cells
Islets
Enzymes
Collagenase Type 4 : 1.3% - 2.0%
Medium
HBSS
Pancreas
Rat
Rat, SD, adult
Li, J. , Zhou, R. , Bie, B. , Huang, N. , Guo, Y. , Chen, H. , Shi, M. , Yang, J. , Zhang, J. and Li, Z.
Emodin and Baicalein Inhibit Sodium Taurocholate-Induced Vacuole Formation in Pancreatic Acinar Cells. ,
World J Gastroenterol
 24 , 35-45 , 2018
Cells
Acinar
Enzymes
Medium
Culture media
Pancreas
Rat
Rat
Williams, J.
Isolation of Rodent Pancreatic Acinar Cells and Acini by Collagenase Digestion ,
Pancreapedia
 , 2010
Cells
Acinar
Enzymes
CLSPA : 100 u/ml
Medium
DMEM
Pancreas
Rat
Rat, Wistar, 300g
Verga Falzacappa, C. , Mangialardo, C. , Raffa, S. , Mancuso, A. , Piergrossi, P. , Moriggi, G. , Piro, S. , Stigliano, A. , Torrisi, M. , Brunetti, E. , Toscano, V. and Misiti, S.
The Thyroid Hormone T3 Improves Function and Survival of Rat Pancreatic Islets During In Vitro Culture. ,
Islets
 2 , 96 , 2010
Cells
Islets
Enzymes
Medium
CMRL 1066
Pancreas
Rat
Rat, adult
Williams, J. , Huang, H. , Kover, K. , Moore, W. , Berkland, C. , Singh, M. , Smirnova, I. , MacGregor, R. and Stehno-Bittel, L.
Reduction of Diffusion Barriers in Isolated Rat Islets Improves Survival, but not Insulin Secretion or Transplantation Outcome. ,
Organogenesis
 6 , 115 , 2010
Cells
Islets
Enzymes
Collagenase Type 1 : 450 u/ml
Medium
Leibowitz L-15
Pancreas
Rat
Rat, male, 7-11 week
Getty-Kaushik, L. , Richard, A. , Deeney, J. , Shirihai, O. and Corkey, B.
The CB1 Antagonist Rimonabant Decreases Insulin Hypersecretion in Rat Pancreatic Islets ,
Obesity
 17 , 1856 , 2009
Cells
Islets
Enzymes
Collagenase Type 4 : see reference
Medium
RPMI 1640

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.