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Tissue References Search Tool

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Neural
Rat
Rat, postnatal
Hatanaka,H. , Tsukui, H. and Nihonmatsu, I.
Septal Cholinergic Neurons From Postnatal Rat Can Survive In The Dissociate Culture Conditions In The Presence Of Nerve Growth Factor ,
Neurosci Lett
 79 , 85 , 1987
Cells
Septal neurons
Enzymes
Papain : 0.05%
Medium
PBS, CMF
Neural
Rat
Rat, SD, female, 4 month old
Wood, P. and Bunge, R.
Evidence That Axons are Mitogenic for Oligodendrocytes Isolated From Adult Animals ,
Nature
 320 , 756 , 1986
Cells
CNS cells
Enzymes
Trypsin : 0.25%
Medium
EBSS
Neural
Rat
Rat, Long Evans, 1-15 days
Huettner, J. and Baughman, R.
Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats ,
J Neurosci
 6 , 3044 , 1986
Cells
Neurons, visual cortex
Enzymes
Papain : 20 u/ml
Medium
BSS
(see reference)
Neural
Rat
Rat, embryo, 15 day
Jahr, C. and Jessell, T.
Synaptic Transmission between Dorsal Root Ganglion and Dorsal Horn Neurons in Culture: Antagonism of Monosynaptic Excitatory Postsynaptic Potentials and Glutamate Excitation by Kynurenate ,
J Neurosci
 5 , 2281 , 1985
Cells
Dorsal horn neurons
Spinal
Enzymes
Trypsin : 0.025%
Medium
Ham's F-12
Neural
Rat
Rat, postnatal
Leifer, D. , Lipton, S. , Barnstable, C. and Masland, R.
Monoclonal Antibody to Thy-1 Enhances Regeneration of Processes by Rat Retinal Ganglion Cells in Culture ,
Science
 224 , 303 , 1984
Cells
Ganglion, retina
Enzymes
Papain : 12.5 u/ml
Medium
HBSS w/5 mM HEPES
Neural
Rat
Rat, fetus, 18-20 day
Bartlett, W. and Banker, G.
An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts ,
J Neurosci
 4 , 1944 , 1984
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.25%
Medium
HBSS, CMF
Neural
Rat
Rat, SD, pups
Sarthy PV, Curtis BM. and Catterall WA.
Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat ,
J Neurosci
 3 (12) , 2532 , 1983
Cells
Retina
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Neural
Rat
Rat, W/FU, 5-8 day
Raff, M. , Fields, K. , Hakomori, S. , Mirsky, R. , Pruss, R. and Winter, J.
Cell-Type-Specific Markers for Distinguishing and Studying Neurons And the Major Classes of Glial Cells in Culture ,
Brain Res
 174 , 283 , 1979
Cells
Neurons and glial
Enzymes
Trypsin : 0.25%
Medium
MEM
(see reference)
Neural
Rat
Rat, fetus
Wakshull, E. , Johnson, M. and Burton, H.
Postnatal Rat Sympathetic Neurons In Culture. 1. A Comparison With Embryonic Neurons ,
J Neurophysiol
 42 , 1410 , 1979
Cells
Neurons, sympathetic
Enzymes
Trypsin : 0.25%
Medium
L-15 or HBSS, CMF
Neural
Rat
Rat, Wistar/Furth, newborn
(also bovine)
Brockes, J. , Fields, K. and Raff, M.
Studies on Cultured Rat Schwann Cells. I. Establishment of Purified Populations From Cultures of Peripheral Nerve ,
Brain Res
 165 , 105 , 1979
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
Neural
Rat
Rat, embryo
Dichter, M.
Rat Cortical Neurons in Cell Culture: Culture Methods, Cell Morphology, Electrophysiology, and Synapse Formation ,
Brain Res
 149 , 279 , 1978
Cells
Neurons, cortical
Enzymes
Trypsin : 0.027%
Medium
MEM
Neural
Rat
Rat, newborn
Reichardt, L. and Patterson, P.
Neurotransmitter Synthesis and Uptake by Isolated Sympathetic Neurones in Microcultures ,
Nature
 270 , 147 , 1977
Cells
Neurons, sympathetic
Enzymes
Medium
Hank's solution, CF
Neural
Rat
Rat, neonatal
Mains, R. and Patterson, P.
Primary Cultures of Dissociated Sympathetic Neurons I. Establishment of Long-Term Growth in Culture and Studies of Differentiated Properties ,
J Cell Biol
 59 , 329 , 1973
Cells
Neurons, superior cervical ganglia
Enzymes
Trypsin : 0.1%
Medium
Basal L-15 medium
Neural
Shellfish
Aplysia californica
Lee, A. , Decourt, B. and Suter, D.
Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones. ,
J Vis Exp
 12 , 662 , 2008
Cells
Neurons
Enzymes
Medium
L-15-ASW
Neural
Shellfish
Snails (Helisoma trivolis), albino, adult
Zoran, M. , Doyle, R. and Haydon, P.
Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis ,
Neuron
 6 , 145 , 1991
Cells
Buccal ganglia; SLT muscle
Enzymes
Trypsin : 0.2%
Medium
DMEM
Neural
Shellfish
Pond snail (Helisoma) albino, adult
Haydon, P.
The Formation of Chemical Synapses Between Cell-Cultured Neuronal Somata ,
J Neurosci
 8 , 1032 , 1988
Cells
Somata, buccal ganglia
Enzymes
Trypsin : 0.2%
Medium
Antibiotic saline, Leibowitz 50%
Neural
Shellfish
Helisoma trivovlis
Cohan, C. , Haydon, P. and Kater, S.
Single Channel Activity Differs in Growing and Nongrowing Growth Cones of Isolated Identified Neurons of Helisoma ,
J Neurosci Res
 13 , 285 , 1985
Cells
Buccal ganglia
Enzymes
Trypsin : 0.2%
Medium
L-15 medium
Neural
Shellfish
Mollusc, juvenile 1-2 gm or adult 50-100 gm
Schacher, S. and Proshanksy, E.
Neurite Regeneration by Aplysia Neurons in Dissociated Cell Culture: Modulation by Aplysia Hemolymph and the Presence of the Initial Axonal Segment ,
J Neurosci
 3 (12) , 2403 , 1983
Cells
Neurons
LUQ cells
Enzymes
Protease : 1%
Medium
L15 medium
Neural
Shellfish
Aplysia californica
Camardo, J. , Proshansky, E. and Schacher, S.
Identified Aplysia Neurons Form Specific Chemical Synapses in Culture ,
J Neurosci
 3 (12) , 2614 , 1983
Cells
LUQ cells
RUQ cells
Enzymes
Protease : 1%
Medium
L15 medium
Pancreas
Rat
Rat, SD, adult
Li, J. , Zhou, R. , Bie, B. , Huang, N. , Guo, Y. , Chen, H. , Shi, M. , Yang, J. , Zhang, J. and Li, Z.
Emodin and Baicalein Inhibit Sodium Taurocholate-Induced Vacuole Formation in Pancreatic Acinar Cells. ,
World J Gastroenterol
 24 , 35-45 , 2018
Cells
Acinar
Enzymes
Medium
Culture media
Pancreas
Rat
Rat
Williams, J.
Isolation of Rodent Pancreatic Acinar Cells and Acini by Collagenase Digestion ,
Pancreapedia
 , 2010
Cells
Acinar
Enzymes
CLSPA : 100 u/ml
Medium
DMEM
Pancreas
Rat
Rat, Wistar, 300g
Verga Falzacappa, C. , Mangialardo, C. , Raffa, S. , Mancuso, A. , Piergrossi, P. , Moriggi, G. , Piro, S. , Stigliano, A. , Torrisi, M. , Brunetti, E. , Toscano, V. and Misiti, S.
The Thyroid Hormone T3 Improves Function and Survival of Rat Pancreatic Islets During In Vitro Culture. ,
Islets
 2 , 96 , 2010
Cells
Islets
Enzymes
Medium
CMRL 1066
Pancreas
Rat
Rat, adult
Williams, J. , Huang, H. , Kover, K. , Moore, W. , Berkland, C. , Singh, M. , Smirnova, I. , MacGregor, R. and Stehno-Bittel, L.
Reduction of Diffusion Barriers in Isolated Rat Islets Improves Survival, but not Insulin Secretion or Transplantation Outcome. ,
Organogenesis
 6 , 115 , 2010
Cells
Islets
Enzymes
Collagenase Type 1 : 450 u/ml
Medium
Leibowitz L-15
Pancreas
Rat
Rat, male, 7-11 week
Getty-Kaushik, L. , Richard, A. , Deeney, J. , Shirihai, O. and Corkey, B.
The CB1 Antagonist Rimonabant Decreases Insulin Hypersecretion in Rat Pancreatic Islets ,
Obesity
 17 , 1856 , 2009
Cells
Islets
Enzymes
Collagenase Type 4 : see reference
Medium
RPMI 1640
Pancreas
Rat
Rat
MacGregor, R. , Williams, S. , Tong, P. , Kover, K. , Moore, W. and Stehno-Bittel, L.
Small Rat Islets are Superior to Large Islets in In Vitro Function and in Transplantation Outcomes. ,
Am J Physiol/Endo
 290 , E771 , 2006
Cells
Islets
Enzymes
Collagenase Type 1 : 450 u/ml
Medium
RPMI 1640
Pancreas
Rat
Rat, Wistar, male, 250-400g
Tian XH, Xue WJ, Ding XM, Pang XL, Teng Y, Tian PX. and Feng XS
Small intestinal submucosa improves islet survival and function during in vitro culture ,
World J Gastroenterol
 11 , 7378 , 2005
Cells
Pancreatic islets
Enzymes
Collagenase : 0.75%
Medium
RPMI 1640
Pancreas
Rat
Rat, 250-350 g
Haefliger Jacques-Antoine, Tawadros Thomas, Meylan Laure, Gurun SabineLe, Roehrich Marc-Estienne, Martin David, Thorens Bernard, Waeber Gerard.
The scaffold protein IB1/JIP-1 is a critical mediator of cytokine-induced apoptosis in pancreatic beta cells ,
J Cell Sci
 116 , 1463-9 , 2003
Cells
Pancreatic islet
Enzymes
Medium
HBSS
Pancreas
Rat
Rat, SD, male
Ji B, Kopin AS, Logsdon CD.
Species differences between rat and mouse CCKA receptors determine the divergent acinar cell response to the cholecystokinin analog JMV-180 ,
J Biol Chem
 275 , 19115-20 , 2000
Cells
Ancinar
Enzymes
CLSPA : see reference
Medium
DMEM
Pancreas
Rat
Rat
Blinman TA, Gukovsky I, Mouria M, Zaninovic V, Livingston E, Pandol SJ, Gukovskaya AS.
Activation of pancreatic acinar cells on isolation from tissue: cytokine upregulation via p38 MAP kinase ,
Am J Physiol Cell Physiol
 279 , C1993-2003 , 2000
Cells
Pancreatic acini
Enzymes
CLSPA : 30 u/ml
Collagenase Type 4 : 30 u/ml
Medium
M199
Pancreas
Rat
Rat
Verspohl, E. and Wienecke, A.
The Role of Protein Kinase C in the Desensitization of Rat Pancreatic Islets to Chrolinergic stimulation ,
J Endocrinol
 159 , 287 , 1998
Cells
Islets
Enzymes
Collagenase : 126 - 196 u/ml
Medium
HBSS
Pancreas
Rat
Rat
Takaki, R. and Ono J
Culture of Pancreatic Islet Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:11.1 , 1995
Cells
Islets
Enzymes
Collagenase : 0.5 - 0.9%
Medium
HBSS
Pancreas
Rat
Rat
Githens, S.
Pancreatic Duct Epithelial Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:12.1 , 1995
Cells
Duct
Enzymes
Papain : 25 u/ml
Medium
DMEM /F-12
Pancreas
Rat
Rat, S-Wistar, male, 230 - 270 g
(also mouse, 6 - 8 wk old)
Ohzato, H. , Gotoh, M. , Monden, M. , Dono, K. , Kanai, T. and Mori, T.
Improvement in the Islet Yield From a Cold-Preserved Pancreas by Pancreatic Ductal Collagenase Distention at the Time of Harvesting ,
Transplantation
 51 , 566 , 1991
Cells
Islets
Enzymes
Collagenase : 0.1% - 0.2%
Medium
HBSS
Pancreas
Rat
Rat, SD, male, 40 - 100 g
Hirschi, K. , Kenny, S. , Justice, J. and Brannon, P.
Effects of Secretin And Caerulein On Enzymes Of Cultured Pancreatic Acinar Cells ,
In Vitro Cell Dev Biol
 27 , 660 , 1991
Cells
Acinar
Enzymes
Hyaluronidase : 462 u/ml
Medium
Ham's F12
Pancreas
Rat
Rat, SD, male, 100 - 150 g
Yeh, C. , Mertz, P. , Oliver, C. , Baum, B. and Kousvelari, E.
Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells ,
In Vitro Cell Dev Biol
 27 , 707 , 1991
Cells
Parotid acinar
Enzymes
Trypsin : 0.001%
Medium
F12 medium
Pancreas
Rat
Rat, Wistar, male
Foskett, J. , Roifman, C. and Wong, D.
Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells ,
J Biol Chem
 266 , 2778 , 1991
Cells
Parotid acinar
Enzymes
Trypsin : 0.02%
Medium
Solution B
(See reference)

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.