Tissue References Search Tool

Rat

Rat, 6 day old

Evans GS, Flint N, Somers AS, Eyden B. and Potten CS.

The development of a method for the preparation of rat intestinal epithelial cell primary cultures ,
J Cell Sci
101 , 219 , 1992

Cells

Rat intestinal epithelial

Enzymes

Neutral Protease : 0.01%

Collagenase : 300 u/ml

Medium

DMEM

Rat

Rat, SD, adult, 90-120 day old, 350-450 g

Klinefelter, G.

A Novel System for the Co-Culture of Epididymal Epithelial Cells and Sperm From Adult Rats ,
J Tiss Cul Meth
14 , 195 , 1992

Cells

Epididymal epithelial

Enzymes

Collagenase Type 2 : 0.1%

Medium

HBSS

Rat

Rat, adult
(also hamster)

Heimann, T. and Githens, S.

Rat Pancreatic Duct Epithelium Cultured on a Porous Support Coated with Extracellular Matrix ,
Pancreas
6 (5) , 514 , 1991

Cells

Interlobular duct fragments

Medium

DMEM/Ham's F-12

Rat

Rat, SD, 200 g

Yassin, R. , Clearfield, H. , Katz, S. and Murthy, S.

Gastrin Induction of mRNA Expression in Rat Colonic Epithelium In Vitro ,
Peptides
12 , 63 , 1991

Cells

Colon

Enzymes

Deoxyribonuclease I : 10 µg/ml

Medium

(see reference)

Rat

Rat, SD, male, 150 - 250 g

Dial, E. , Kao, Y. and Lichtenberger, L.

Effects of 16,16-Dimethyl Prostaglandin E2 On Glycoprotein And Lipid Synthesis of Gastric Epithelial Cells Grown in a Primary Culture ,
In Vitro Cell Dev Biol
27 , 39 , 1991

Cells

Epithelial

Stomach

Enzymes

Pronase : 0.15%

Medium

Medium 199

Rat

Rodent, various (see reference)

Robinson, C. and Wu, R.

Culture of Conducting Airway Epithelial Cells in Serum-Free Medium ,
J Tiss Cul Meth
13 , 95 , 1991

Cells

Epithelial

Enzymes

Trypsin : 0.2%

Medium

MEM, PBS

Rat

Rat, 6-8 day

Chang CW, Roque RS, Defoe DM. and Caldwell RB.

An improved method for isolation and culture of pigment epithelial cells from rat retina ,
Curr Eye Res
10(11) , 1081 , 1991

Cells

Retinal pigment epithelial

Enzymes

Neutral Protease : 2%

Medium

DMEM

Rat

Rat, Wistar, neonatal

Jassal, D. , Han, R. , Caniggia, I. , Post, M. and Tanswell, A.

Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium ,
In Vitro Cell Dev Biol
27A , 625 , 1991

Cells

Epithelial

Enzymes

Trypsin : 0.1%

Medium

HBSS

Rat

Rat (ACI/NMs X BUF/Mna) F1, male, 28 months
Rat (ACI/MNs) male, 8 weeks

Masuda, A. , Ohtsuka, K. and Matsuyama, M.

Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus ,
In Vitro Cell Dev Biol
26 , 713 , 1990

Cells

Epthelial

Enzymes

Collagenase Type 3 : 0.1%

Medium

Eagle's MEM Serum-free

Rat

Rat, Fisher 344, male, 8 wks old,
250-300 g

Chang, L. , Wu, R. and Nettesheim, P.

Morphological Changes in Rat Tracheal Cells During The Adaptive and Early Growth Phase in Primary Cell Culture ,
J Cell Sci
74 , 283 , 1985

Cells

Tracheal epithelial

Enzymes

Pronase : 0.5%

Medium

DMEM

Rat

Rat, Fischer, male, 4-6 weeks

Babcock, M. , Marino, M. , Gunning, W. and Stoner, G.

Clonal Growth and Serial Propagation of Rat Esophageal Epithelial Cells ,
In Vitro
19 , 403 , 1983

Cells

Epithelial

Esophagus

Enzymes

Hyaluronidase : 0.1%

Medium

HEPES BSS

Rat

Rat, Fischer, Lewis and SD, male, 10-18 months

Herring, A. , Raychaudhuri, R. , Kelley, S. and Iybe, P.

Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats ,
In Vitro
19 , 576 , 1983

Cells

Epithelial

Enzymes

Trypsin : 0.05%

Medium

HBSS CMF

Rat

Rat, SD, female

Cohen, L.

Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma ,
In Vitro
18 , 565 , 1982

Cells

Epithelial, cancer and tumor

Enzymes

Collagenase : 0.1%

Medium

Eagles's MEM

Rat

Rat, Wistar, 12 day

Malan-Shibley, L. and Iype, P.

Influence of Cultures on Cell Morphology/Tyrosine Aminotransferase Levels ,
Exp Cell Res
131 , 363 , 1981

Cells

Epithelial

Enzymes

Trypsin : 0.05%

Medium

HBSS, CMF

Rat

Rat, Fischer, adult, 200-250 g

Williams, G. and Gunn, J.

Long-Term Culture of Adult Rat Liver Epithelial Cells ,
Exp Cell Res
89 , 139 , 1974

Cells

Epithelial

Enzymes

Hyaluronidase : 0.0075%

Medium

KCl-NaCl HEPES Buffer

Rat

Rat, Fischer, 10 day

Williams, G. , Weisburger, E. and Weisburger, J.

Isolation and Long-Term Cell Culture of Epithelial-Like Cells from Rat Liver ,
Exp Cell Res
69 , 106 , 1971

Cells

Epithelial-like

Enzymes

Trypsin : 0.25%

Medium

PBS

Rat

Rat, SD, 1-2 day

Lian, W. , Hu, X. , Zhang, J. , Wu, Y. , Zhao, N. , Ma, H. , He, H. and Lu, Q.

Fucoxanthin Protects Retinal Ganglion Cells and Promotes Parkin-mediated Mitophagy against Glutamate Excitotoxicity. ,
Neuroreport
34 , 385-394 , 2023

Cells

Retinal ganglion

Enzymes

Papain : 0.5%

Deoxyribonuclease I : 0.1%

Medium

MEM

Rat

Adult, female, 170-200 g

Mead, B. and Tomarev, S.

Bone Marrow-Derived Mesenchymal Stem Cells-Derived Exosomes Promote Survival of Retinal Ganglion Cells Through miRNA-Dependent Mechanisms. ,
Stem Cells Transl Med
6 , 1273-1285 , 2017

Cells

Retinal

Enzymes

PDS kit : with modifications

Medium

EBSS

Rat

Rat, Wister, 3-30 wk

Cameron, M. , Kekesi, O. , Morley, J. , Tapson, J. , Breen, P. , Van Schaik, A. and Buskila, Y.

Calcium Imaging of AM Dyes Following Prolonged Incubation in Acute Neuronal Tissue. ,
PLoS ONE
11 , e0155468 , 2016

Cells

Retinal

Enzymes

Papain : 30 u/ml

Deoxyribonuclease I : 0.005%

Medium

EBSS

Rat

Rat, SD, 6-8 week

Vigneswara, V. , Esmaeili, M. , Deer, L. , Berry, M. , Logan, A. and Ahmed, Z.

Eye Drop Delivery of Pigment Epithelium-Derived Factor-34 Promotes Retinal Ganglion Cell Neuroprotection and Axon Regeneration. ,
Mol Cell Neurosci
68 , 212-21 , 2015

Cells

Retinal

Enzymes

PDS kit : per instructions

Medium

EBSS

Rat

Rat, SD

Tan, C. , Andrews, M. , Kwok, J. , Heintz, T. , Gumy, L. , Fassler, R. and Fawcett, J.

Kindlin-1 Enhances Axon Growth on Inhibitory Chondroitin Sulfate Proteoglycans and Promotes Sensory Axon Regeneration. ,
J Neurosci
32 , 7325 , 2012

Cells

Retinal ganglion

Enzymes

PDS kit : with modifications

Medium

Neurobasal

Rat

Rat, SD, 180-220g

Ma, J. , Yu, W. , Wang, Y. , Cao, G. , Cai, S. , Chen, X. , Yan, N. , Yuan, Y. , Zeng, H. , Fleenor, D. , Liu, X. and Pang, I.

Neuroprotective Effects of C-type Natriuretic Peptide on Rat Retinal Ganglion Cells. ,
Inv Ophthal Visual Sci
51 , 3544-53 , 2010

Cells

Retinal ganglion

Enzymes

Papain : 0.2%

Medium

Neurobasal

Rat

Rat, Fisher, adult

Suzuki, T. , Mandai, M. , Akimoto, M. , Yoshimura, N. and Takahashi, M.

The Simultaneous Treatment of MMP-2 Stimulants in Retinal Transplantation Enhances Grafted Cell Migration into the Host Retina. ,
Stem Cells
24 , 2406 , 2006

Cells

Retinal

Enzymes

PDS kit : per instructions

Medium

MEM

Rat

Rat, Dark Agouti, 3-4 week

Akagi T, Mandai M, Ooto S, Hirami Y, Osakada F, Kageyama R, Yoshimura N, Takahashi M.

Otx2 homeobox gene induces photoreceptor-specific phenotypes in cells derived from adult iris and ciliary tissue ,
Inv Ophthal Visual Sci
45 , 4570 , 2004

Cells

Ciliary-derived eye

Enzymes

PDS kit : with modifications

Medium

DMEM/F12

Rat

Rat, embryonic 17 day

Seigel, G.

The Golden Age of Retinal Cell Culture ,
Mol Vis
5 , 4 , 1999

Cells

Retinal

Enzymes

Trypsin : 0.6%

Medium

DMEM

Rat

Rat, SD

Jing, S. , Wen, D. , Yu, Y. , Holst, P. , Luo, Y. , Fang, M. , Tamir, R. , Antonio, L. , Hu, Z. and et al.

GDNF-Induced Activation of the Ret Protein Tyrosine Kinase is Mediated by GDNFR-a, a Novel Receptor for GDNF ,
Cell
85 , 1113 , 1996

Cells

Retina

Enzymes

Papain : 120 U

Medium

HBSS, PBS

Rat

Rat, 4-14 week

Wang N, Koutz CA. and Anderson RE.

A method for the isolation of retinal pigment epithelial cells from adult rats ,
Inv Ophthal Visual Sci
34(1) , 101 , 1993

Cells

Retinal pigment epithelial cells

Enzymes

Collagenase Type 1 : 65 u/ml

Hyaluronidase : 220 u/ml

Medium

CF Hanks with EDTA

Rat

Rat, 6-8 day

Chang CW, Roque RS, Defoe DM. and Caldwell RB.

An improved method for isolation and culture of pigment epithelial cells from rat retina ,
Curr Eye Res
10(11) , 1081 , 1991

Cells

Retinal pigment epithelial

Enzymes

Neutral Protease : 2%

Medium

DMEM

Rat

Rat, Long Evans, 1-15 days

Huettner, J. and Baughman, R.

Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats ,
J Neurosci
6 , 3044 , 1986

Cells

Neurons, visual cortex

Enzymes

Papain : 20 u/ml

Medium

BSS

(see reference)

Rat

Rat, SD, pups

Sarthy PV, Curtis BM. and Catterall WA.

Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat ,
J Neurosci
3 (12) , 2532 , 1983

Cells

Retina

Enzymes

Trypsin : 0.25%

Medium

Ham's F-12

Rat

Rat, 1-2 day

Yang, M. , Abudureyimu, M. , Wang, X. , Zhou, Y. , Zhang, Y. and Ren, J.

PHB2 ameliorates Doxorubicin-induced cardiomyopathy through interaction with NDUFV2 and restoration of mitochondrial complex I function. , Redox Biol Vol. 65 , 102812 , 2023

Cells

Cardiomyocytes

Enzymes

Collagenase Type 1 : 0.08%

Medium

DMEM

Rat

Rat, adult

Zhang, Y. , Wang, Y. , Yanni, J. , Qureshi, M. , Logantha, S. , Kassab, S. , Boyett, M. , Gardiner, N. , Sun, H. , Howarth, F. and Dobrzynski, H.

Electrical Conduction System Remodeling in Streptozotocin-Induced Diabetes Mellitus Rat Heart. ,
Front Physiol
10 , 826 , 2019

Cells

Sinoatrial nodal

Enzymes

Collagenase Type 4 : 220 u/ml

Elastase : 1.4 u/ml

Protease : 0.45 u/ml

Medium

Tyrode solution

Rat

Rat, adult

Savi, M. , Bocchi, L. , Mena, P. , Dall'Asta, M. , Crozier, A. , Brighenti, F. , Stilli, D. and Del Rio, D.

In vivo Administration of Urolithin A and B Prevents the Occurrence of Cardiac Dysfunction in Streptozotocin-induced Diabetic Rats. ,
Cardiovasc Diabetol
16 , 80 , 2017

Cells

Cardiomyocytes

Enzymes

Collagenase Type 2 : 0.1%

Protease Type XIV : 0.01%

Medium

Tyrode solution

Rat

Rat, SD, 2 day

Abdul-Ghani, M. , Suen, C. , Jiang, B. , Deng, Y. , Weldrick, J. , Putinski, C. , Brunette, S. , Fernando, P. , Lee, T. , Flynn, P. , Leenen, F. , Burgon, P. Stewart, D. and Megeney, L.

Cardiotrophin 1 Stimulates Beneficial Myogenic and Vascular Remodeling of the Heart. ,
Cell Res
27 , 1195-1215 , 2017

Cells

Cardiomyocytes

Enzymes

Collagenase Type 2 : 200 u/ml

Medium

DMEM