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Tissue References Search Tool

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Epithelial
Rat
Rat
Mei, N. , McDaniel, L. , Dobrovolsky, V. , Guo, X. , Shaddock, J. , Mittelstaedt, R. , Azuma, M. , Shelton, S. , McGarrity, L. , Doerge, D. and Heflich, R.
The Genotoxicity of Acrylamide and Glycidamide in Big Blue Rats. ,
Toxicol Sci
 115 , 412 , 2010
Cells
Mammary epithelial
Enzymes
Medium
HBSS
Epithelial
Rat
Rat, SD, adult, male, 8-10 weeks old
Abou-Haila, A. and Tulsiani, D.
Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization ,
Biol Proced Online
 3 (1) , 35 , 2001
Cells
Seminiferous tubules
Enzymes
Trypsin : 0.05%
Medium
Krebs-Ringer bicarbonate buffer
(see reference)
Epithelial
Rat
Rat, male
Planus, E. , Galiacy, S. , Matthay, M. , Laurent, V. , Gavrilovic, J. , Murphy, G. , Clerici, C. , Isabey, D. , Lafuma, C. and d'Ortho, M.
Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair ,
J Cell Sci
 112 , 243 , 1999
Cells
Alveolar epithelial
Enzymes
Elastase : 40 u/ml
Medium
DMEM
Epithelial
Rat
Rat, embryonic
Shannon JM, Gebb SA. and Nielsen LD
Induction of alveolar type II cell differentiation in embryonic tracheal epithelium in mesenchyme-free culture ,
Development
 126 , 1675 , 1999
Cells
Tracheal epithelial
Enzymes
Medium
DMEM/F12
Epithelial
Rat
Rat, fetal, 14-15 day
Simon-Assmann, P. and Kedinger, M
Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995
Cells
Intestinal mesenchymal and epithelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Epithelial
Rat
Rat, 4-14 week
Wang N, Koutz CA. and Anderson RE.
A method for the isolation of retinal pigment epithelial cells from adult rats ,
Inv Ophthal Visual Sci
 34(1) , 101 , 1993
Cells
Retinal pigment epithelial cells
Enzymes
Collagenase Type 1 : 65 u/ml
Hyaluronidase : 220 u/ml
Medium
CF Hanks with EDTA
Epithelial
Rat
Rat, 6 day old
Evans GS, Flint N, Somers AS, Eyden B. and Potten CS.
The development of a method for the preparation of rat intestinal epithelial cell primary cultures ,
J Cell Sci
 101 , 219 , 1992
Cells
Rat intestinal epithelial
Enzymes
Collagenase : 300 u/ml
Medium
DMEM
Epithelial
Rat
Rat, SD, adult, 90-120 day old, 350-450 g
Klinefelter, G.
A Novel System for the Co-Culture of Epididymal Epithelial Cells and Sperm From Adult Rats ,
J Tiss Cul Meth
 14 , 195 , 1992
Cells
Epididymal epithelial
Enzymes
Medium
HBSS
Epithelial
Rat
Rat, adult
(also hamster)
Heimann, T. and Githens, S.
Rat Pancreatic Duct Epithelium Cultured on a Porous Support Coated with Extracellular Matrix ,
Pancreas
 6 (5) , 514 , 1991
Cells
Interlobular duct fragments
Medium
DMEM/Ham's F-12
Epithelial
Rat
Rat, SD, 200 g
Yassin, R. , Clearfield, H. , Katz, S. and Murthy, S.
Gastrin Induction of mRNA Expression in Rat Colonic Epithelium In Vitro ,
Peptides
 12 , 63 , 1991
Cells
Colon
Enzymes
Deoxyribonuclease I : 10 µg/ml
Medium
(see reference)
Epithelial
Rat
Rat, SD, male, 150 - 250 g
Dial, E. , Kao, Y. and Lichtenberger, L.
Effects of 16,16-Dimethyl Prostaglandin E2 On Glycoprotein And Lipid Synthesis of Gastric Epithelial Cells Grown in a Primary Culture ,
In Vitro Cell Dev Biol
 27 , 39 , 1991
Cells
Epithelial
Stomach
Enzymes
Pronase : 0.15%
Medium
Medium 199
Epithelial
Rat
Rodent, various (see reference)
Robinson, C. and Wu, R.
Culture of Conducting Airway Epithelial Cells in Serum-Free Medium ,
J Tiss Cul Meth
 13 , 95 , 1991
Cells
Epithelial
Enzymes
Trypsin : 0.2%
Medium
MEM, PBS
Epithelial
Rat
Rat, 6-8 day
Chang CW, Roque RS, Defoe DM. and Caldwell RB.
An improved method for isolation and culture of pigment epithelial cells from rat retina ,
Curr Eye Res
 10(11) , 1081 , 1991
Cells
Retinal pigment epithelial
Enzymes
Medium
DMEM
Epithelial
Rat
Rat, Wistar, neonatal
Jassal, D. , Han, R. , Caniggia, I. , Post, M. and Tanswell, A.
Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium ,
In Vitro Cell Dev Biol
 27A , 625 , 1991
Cells
Epithelial
Enzymes
Trypsin : 0.1%
Medium
HBSS
Epithelial
Rat
Rat (ACI/NMs X BUF/Mna) F1, male, 28 months
Rat (ACI/MNs) male, 8 weeks
Masuda, A. , Ohtsuka, K. and Matsuyama, M.
Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus ,
In Vitro Cell Dev Biol
 26 , 713 , 1990
Cells
Epthelial
Enzymes
Medium
Eagle's MEM Serum-free
Epithelial
Rat
Rat, Fisher 344, male, 8 wks old,
250-300 g
Chang, L. , Wu, R. and Nettesheim, P.
Morphological Changes in Rat Tracheal Cells During The Adaptive and Early Growth Phase in Primary Cell Culture ,
J Cell Sci
 74 , 283 , 1985
Cells
Tracheal epithelial
Enzymes
Pronase : 0.5%
Medium
DMEM
Epithelial
Rat
Rat, Fischer, male, 4-6 weeks
Babcock, M. , Marino, M. , Gunning, W. and Stoner, G.
Clonal Growth and Serial Propagation of Rat Esophageal Epithelial Cells ,
In Vitro
 19 , 403 , 1983
Cells
Epithelial
Esophagus
Enzymes
Hyaluronidase : 0.1%
Medium
HEPES BSS
Epithelial
Rat
Rat, Fischer, Lewis and SD, male, 10-18 months
Herring, A. , Raychaudhuri, R. , Kelley, S. and Iybe, P.
Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats ,
In Vitro
 19 , 576 , 1983
Cells
Epithelial
Enzymes
Trypsin : 0.05%
Medium
HBSS CMF
Epithelial
Rat
Rat, SD, female
Cohen, L.
Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma ,
In Vitro
 18 , 565 , 1982
Cells
Epithelial, cancer and tumor
Enzymes
Collagenase : 0.1%
Medium
Eagles's MEM
Epithelial
Rat
Rat, Wistar, 12 day
Malan-Shibley, L. and Iype, P.
Influence of Cultures on Cell Morphology/Tyrosine Aminotransferase Levels ,
Exp Cell Res
 131 , 363 , 1981
Cells
Epithelial
Enzymes
Trypsin : 0.05%
Medium
HBSS, CMF
Epithelial
Rat
Rat, Fischer, adult, 200-250 g
Williams, G. and Gunn, J.
Long-Term Culture of Adult Rat Liver Epithelial Cells ,
Exp Cell Res
 89 , 139 , 1974
Cells
Epithelial
Enzymes
Hyaluronidase : 0.0075%
Medium
KCl-NaCl HEPES Buffer
Epithelial
Rat
Rat, Fischer, 10 day
Williams, G. , Weisburger, E. and Weisburger, J.
Isolation and Long-Term Cell Culture of Epithelial-Like Cells from Rat Liver ,
Exp Cell Res
 69 , 106 , 1971
Cells
Epithelial-like
Enzymes
Trypsin : 0.25%
Medium
PBS
Muscle
Rat
Rat, day 7
Vilmont, V. , Cadot, B. , Ouanoumou, G. and Gomes, E.
A System for Studying Mechanisms of Neuromuscular Junction Development and Maintence ,
Development
 143 , 2464 , 2016
Cells
Skeletal muscle
Enzymes
Collagenase : 0.05%
Medium
DMEM
Muscle
Rat
Rat, SD
Zeng, Y. , Liu, H. , Kang, K. , Wang, Z. , Hui, G. , Zhang, X. , Zhong, J. , Peng, W. , Ramchandran, R. , Raj, J. and Gou, D.
Hypoxia Inducible Factor-1 Mediates Expression of miR-322: Potential Role in Proliferation and Migration of Pulmonary Arterial Smooth Muscle Cells. ,
Sci Rep
 5 , 12098 , 2015
Cells
Pulmonary arterial smooth muscle
Enzymes
Elastase : 0.05%
Medium
Buffer
Muscle
Rat
Rat
Weisleder, N. , Zhou, J. and Ma, J.
Detection of Calcium Sparks in Intact and Permeabilized Skeletal Muscle Fibers. ,
Methods Mol Biol
 798 , 395 , 2012
Cells
Skeletal muscle fiber
Enzymes
Medium
Tyrode
Muscle
Rat
Rat
Weber, S. , Gratopp, A. , Akanbi, S. , Rheinlaender, C. , Sallmon, H. , Barikbin, P. and Koehne, P.
Isolation and Culture of Fibroblasts, Vascular Smooth Muscle, and Endothelial Cells From the Fetal Rat Ductus Arteriosus ,
Pediatr Res
 70 , 236 , 2011
Cells
Vascular smooth muscle
Enzymes
Medium
DMEM
Muscle
Rat
Rat, fetal 17-18 day
Das, M. , Rumsey, J. , Bhargava, N. , Stancescu, M. and Hickman, J.
Skeletal Muscle Tissue Engineering: A Maturation Model Promoting Long-Term Survival of Myotubes, Structural Development of the Excitation-Contraction Coupling Apparatus and Neonatal Myosin Heavy Chain Expression. ,
Biomaterials
 30 , 5392 , 2009
Cells
Myotubes
Enzymes
Trypsin : 0.05%
Medium
see reference
Muscle
Rat
Rat
Pacak, C. and Cowan, D.
Fabrication of Myogenic Engineered Tissue Constructs. ,
J Vis Exp
 27 , e1137 , 2009
Cells
Myogenic
Enzymes
Medium
Ham's F-10
Muscle
Rat
Rat
Larkin, L. , Calve, S. , Kostrominova, T. and Arruda, E.
Structure and Functional Evaluation of Tendon-Skeletal Muscle Constructs Engineered In Vitro. ,
Tiss Eng
 12 , 3149-58 , 2006
Cells
myogenic
Enzymes
Neutral Protease : 8 u/ml
Collagenase Type 4 : 200 u/ml
Medium
DMEM
Muscle
Rat
Rat, Lewis, neonatal
Kim, J. , Hadlock, T. , Cheney, M. , Varvares, M. and Marler, J.
Muscle Tissue Engineering for Partial Glossectomy Defects. ,
Arch Facial Plast Surg
 5 , 403 , 2003
Cells
Myoblasts
Enzymes
Medium
Ham's F-10
Muscle
Rat
Rat, SD, adult and neonatal w/in 1 day of birth
Dennis, R. , Kosnik II, P. , Gilbert, M. and Faulkner, J.
Excitability and Contractility of Skeletal Muscle Engineered from Primary Cultures and Cell Lines ,
Am J Physiol Cell Physiol
 280 , C288 , 2001
Cells
Myooids
Enzymes
Neutral Protease : 4 u/ml
Medium
Ham's F-12
Muscle
Rat
Rat, SD, 250 g
Jaggar JH.
Intravascular pressure regulates local and global Ca(2+) signaling in cerebral artery smooth muscle cells ,
Am J Physiol Cell Physiol
 281 , C439-48 , 2001
Cells
Arterial smooth muscle
Enzymes
Papain : 0.03%
Collagenase : 0.1%
Medium
see reference
Muscle
Rat
Rat, SD, 3 month
Stevenson S. , Rollence M. , Marshall-Neff J. and Liau G.
A Genetically Modified Adenoviral Vector Exhibits Enhanced Gene Transfer of Human Smooth Muscle Cells ,
J Vasc Res
 38(5) , 471 , 2001
Cells
Smooth muscle cells
Enzymes
Medium
M-199
Muscle
Rat
Rat, Wistar, adult, male
Wellman, G. , Barrett-Jolley, R. , Koppel, H. Everitt, D. and Quayle, J.
Inhibition of Vascular KATP Channels by U-37883A: A Comparison with Cardiac and Skeletal Muscle ,
Br J Pharmacol
 128 , 909 , 1999
Cells
Smooth & skeletal muscle
Cardiac myocytes
Enzymes
Protease : 0.01%
Medium
PSS
Muscle
Rat
Rat, SD, male, 250-350 g
Hrometz, S. , Edelmann, S. , McCune, D. , Olges, J. , Hadley, R. , Perez ,D. and Piascik, M.
Expression of Multiple Alpha1-Adrenoceptors on Vascular Smooth Muscle: Correlation with the Regulation of Contraction ,
J Pharmacol Exp Ther
 290(1) , 452 , 1999
Cells
Vascular smooth muscle
Enzymes
Elastase : 0.0125%
Medium
DMEM
Muscle
Rat
Rat, SD, male, 150-175 g
Redmond, E. , Cahill, P. and Sitzmann, J.
Perfused Transcapillary Smooth Muscle and Endothelial Cell Co-Culture-A Novel In Vitro Model ,
In Vitro Cell Dev Biol Anim
 31 , 601 , 1995
Cells
Smooth muscle, endothelial
Enzymes
Trypsin : 0.04%
Medium
MEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.