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Tissue References Search Tool

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Eye
Rat

Rat, SD, 1-2 day

Lian, W. , Hu, X. , Zhang, J. , Wu, Y. , Zhao, N. , Ma, H. , He, H. and Lu, Q.
Fucoxanthin Protects Retinal Ganglion Cells and Promotes Parkin-mediated Mitophagy against Glutamate Excitotoxicity. ,
Neuroreport
 34 , 385-394 , 2023
Cells
Retinal ganglion
Enzymes
Papain : 0.5%
Medium
MEM
Eye
Rat
Adult, female, 170-200 g
Mead, B. and Tomarev, S.
Bone Marrow-Derived Mesenchymal Stem Cells-Derived Exosomes Promote Survival of Retinal Ganglion Cells Through miRNA-Dependent Mechanisms. ,
Stem Cells Transl Med
 6 , 1273-1285 , 2017
Cells
Retinal
Enzymes
PDS kit : with modifications
Medium
EBSS
Eye
Rat
Rat, Wister, 3-30 wk
Cameron, M. , Kekesi, O. , Morley, J. , Tapson, J. , Breen, P. , Van Schaik, A. and Buskila, Y.
Calcium Imaging of AM Dyes Following Prolonged Incubation in Acute Neuronal Tissue. ,
PLoS ONE
 11 , e0155468 , 2016
Cells
Retinal
Enzymes
Papain : 30 u/ml
Medium
EBSS
Eye
Rat
Rat, SD, 6-8 week
Vigneswara, V. , Esmaeili, M. , Deer, L. , Berry, M. , Logan, A. and Ahmed, Z.
Eye Drop Delivery of Pigment Epithelium-Derived Factor-34 Promotes Retinal Ganglion Cell Neuroprotection and Axon Regeneration. ,
Mol Cell Neurosci
 68 , 212-21 , 2015
Cells
Retinal
Enzymes
PDS kit : per instructions
Medium
EBSS
Eye
Rat
Rat, SD
Tan, C. , Andrews, M. , Kwok, J. , Heintz, T. , Gumy, L. , Fassler, R. and Fawcett, J.
Kindlin-1 Enhances Axon Growth on Inhibitory Chondroitin Sulfate Proteoglycans and Promotes Sensory Axon Regeneration. ,
J Neurosci
 32 , 7325 , 2012
Cells
Retinal ganglion
Enzymes
PDS kit : with modifications
Medium
Neurobasal
Eye
Rat
Rat, SD, 180-220g
Ma, J. , Yu, W. , Wang, Y. , Cao, G. , Cai, S. , Chen, X. , Yan, N. , Yuan, Y. , Zeng, H. , Fleenor, D. , Liu, X. and Pang, I.
Neuroprotective Effects of C-type Natriuretic Peptide on Rat Retinal Ganglion Cells. ,
Inv Ophthal Visual Sci
 51 , 3544-53 , 2010
Cells
Retinal ganglion
Enzymes
Papain : 0.2%
Medium
Neurobasal
Eye
Rat
Rat, Fisher, adult
Suzuki, T. , Mandai, M. , Akimoto, M. , Yoshimura, N. and Takahashi, M.
The Simultaneous Treatment of MMP-2 Stimulants in Retinal Transplantation Enhances Grafted Cell Migration into the Host Retina. ,
Stem Cells
 24 , 2406 , 2006
Cells
Retinal
Enzymes
PDS kit : per instructions
Medium
MEM
Eye
Rat
Rat, Dark Agouti, 3-4 week
Akagi T, Mandai M, Ooto S, Hirami Y, Osakada F, Kageyama R, Yoshimura N, Takahashi M.
Otx2 homeobox gene induces photoreceptor-specific phenotypes in cells derived from adult iris and ciliary tissue ,
Inv Ophthal Visual Sci
 45 , 4570 , 2004
Cells
Ciliary-derived eye
Enzymes
PDS kit : with modifications
Medium
DMEM/F12
Eye
Rat
Rat, embryonic 17 day
Seigel, G.
The Golden Age of Retinal Cell Culture ,
Mol Vis
 5 , 4 , 1999
Cells
Retinal
Enzymes
Trypsin : 0.6%
Medium
DMEM
Eye
Rat
Rat, SD
Jing, S. , Wen, D. , Yu, Y. , Holst, P. , Luo, Y. , Fang, M. , Tamir, R. , Antonio, L. , Hu, Z. and et al.
GDNF-Induced Activation of the Ret Protein Tyrosine Kinase is Mediated by GDNFR-a, a Novel Receptor for GDNF ,
Cell
 85 , 1113 , 1996
Cells
Retina
Enzymes
Papain : 120 U
Medium
HBSS, PBS
Eye
Rat
Rat, 4-14 week
Wang N, Koutz CA. and Anderson RE.
A method for the isolation of retinal pigment epithelial cells from adult rats ,
Inv Ophthal Visual Sci
 34(1) , 101 , 1993
Cells
Retinal pigment epithelial cells
Enzymes
Collagenase Type 1 : 65 u/ml
Hyaluronidase : 220 u/ml
Medium
CF Hanks with EDTA
Eye
Rat
Rat, 6-8 day
Chang CW, Roque RS, Defoe DM. and Caldwell RB.
An improved method for isolation and culture of pigment epithelial cells from rat retina ,
Curr Eye Res
 10(11) , 1081 , 1991
Cells
Retinal pigment epithelial
Enzymes
Medium
DMEM
Eye
Rat
Rat, Long Evans, 1-15 days
Huettner, J. and Baughman, R.
Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats ,
J Neurosci
 6 , 3044 , 1986
Cells
Neurons, visual cortex
Enzymes
Papain : 20 u/ml
Medium
BSS
(see reference)
Eye
Rat
Rat, SD, pups
Sarthy PV, Curtis BM. and Catterall WA.
Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat ,
J Neurosci
 3 (12) , 2532 , 1983
Cells
Retina
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Heart
Rat

Rat, 1-2 day

Yang, M. , Abudureyimu, M. , Wang, X. , Zhou, Y. , Zhang, Y. and Ren, J.
PHB2 ameliorates Doxorubicin-induced cardiomyopathy through interaction with NDUFV2 and restoration of mitochondrial complex I function. , Redox Biol Vol. 65 , 102812 , 2023
Cells
Cardiomyocytes
Enzymes
Medium
DMEM
Heart
Rat
Rat, adult
Zhang, Y. , Wang, Y. , Yanni, J. , Qureshi, M. , Logantha, S. , Kassab, S. , Boyett, M. , Gardiner, N. , Sun, H. , Howarth, F. and Dobrzynski, H.
Electrical Conduction System Remodeling in Streptozotocin-Induced Diabetes Mellitus Rat Heart. ,
Front Physiol
 10 , 826 , 2019
Cells
Sinoatrial nodal
Enzymes
Collagenase Type 4 : 220 u/ml
Elastase : 1.4 u/ml
Protease : 0.45 u/ml
Medium
Tyrode solution
Heart
Rat
Rat, adult
Savi, M. , Bocchi, L. , Mena, P. , Dall'Asta, M. , Crozier, A. , Brighenti, F. , Stilli, D. and Del Rio, D.
In vivo Administration of Urolithin A and B Prevents the Occurrence of Cardiac Dysfunction in Streptozotocin-induced Diabetic Rats. ,
Cardiovasc Diabetol
 16 , 80 , 2017
Cells
Cardiomyocytes
Enzymes
Protease Type XIV : 0.01%
Medium
Tyrode solution
Heart
Rat
Rat, SD, 2 day
Abdul-Ghani, M. , Suen, C. , Jiang, B. , Deng, Y. , Weldrick, J. , Putinski, C. , Brunette, S. , Fernando, P. , Lee, T. , Flynn, P. , Leenen, F. , Burgon, P. Stewart, D. and Megeney, L.
Cardiotrophin 1 Stimulates Beneficial Myogenic and Vascular Remodeling of the Heart. ,
Cell Res
 27 , 1195-1215 , 2017
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 200 u/ml
Medium
DMEM
Heart
Rat
Rat, 250 g
Stoppel, W. , Gao, A. , Greaney, A. , Partlow, B. , Bretherton, R. , Kaplan, D. and Black, L
Elastic, Silk-Cardiac Extracellular Matrix Hydrogels Exhibit Time-Dependent Stiffening that Modulates Cardiac Fibroblast Response. ,
J Biomed Mater Res A
 104 , 3058 , 2016
Cells
Fibroblasts
Enzymes
Collagenase Type 2 : 300 u/ml
Medium
DMEM
Heart
Rat
Rat, 1-2 day
Xie, L. , Pi, X. , Townley-Tilson, WH. , Li, N. , Wehrens, X. , Entman, M. , Taffet, G. , Mishra, A. , Peng, J. , Schisler, J. , Meissner, G. and Patterson, C.
PHD2/3-dependent Hydroxylation Tunes Cardiac Response to β-adrenergic Stress via Phospholamban. ,
J Clin Invest
 125 , 2759-71 , 2015
Cells
Cardiomyocytes
Enzymes
NCIS kit : per instructions
Medium
MEM
Heart
Rat
Rat, SD, 8-10 week
Lee, J. , Ha, J. and Leem, C.
A Novel Nicotinamide Adenine Dinucleotide Correction Method for Mitochondrial Ca(2+) Measurement with FURA-2-FF in Single Permeabilized Ventricular Myocytes of Rat. ,
Korean J Physiol Pharmacol
 19 , 373-82 , 2015
Cells
Ventricular myocytes
Enzymes
Collagenase : 0.08%
Pronase : 0.004%
Medium
Tyrode's Solution
Heart
Rat
Rat, neonatal
Song, D. , Lee, K. , Ryu, J. , Jeon, H. and Kim, D.
The Molecular Interaction of Heart LIM Protein (HLP) with RyR2 and Caveolin-3 is Essential for Ca(2+)-Induced Ca(2+) Release in the Heart. ,
Biochem Biophys Res Commun
 463 , 975-81 , 2015
Cells
Cardiomyocytes
Enzymes
NCIS kit : per instructions
Medium
HBSS
Heart
Rat
Rat, Wistar, 250-350g
Calderon-Sachez, E. , Domi­nguez-Rodriguez, A. , Lopez-Haldon, J. , Jimenez-Navarro, M. , Gomez, A. , Smani, T. and Ordonez, A.
Cardioprotective Effect of Ranolazine in the Process of Ischemia-reperfusion in Adult Rat Cardiomyocytes. ,
Rev Esp Cardiol (Engl Ed)
 , 2015
Cells
Ventricular myocytes
Enzymes
Collagenase Type 2 : 251 u/ml
Medium
Tyrode solution
Heart
Rat
Rat, SD, 2-3 day
Fang, R, Qiao, S. , Liu, Y. , Meng, Q. , Chen, X. , Song, B. , Hou, X. and Tian, W.
Sustained Co-Delivery of BIO and IGF-1 by a Novel Hybrid Hydrogel System to Stimulate Endogenous Cardiac Repair in Myocardial Infarcted Rat Hearts. ,
Int J Nanomedicine
 10 , 4691-703 , 2015
Cells
Cardiomyocytes
Enzymes
Medium
DMEM
Heart
Rat
Rat, SD
Horne, T. , VandeKopple, M. , Sauls, K. , Koenig, S. , Anstine, L. , Garg, V. , Norris, R. and Lincoln, J.
Dynamic Heterogeneity of the Heart Valve Interstitial Cell Population in Mitral Valve Health and Disease ,
J Cardiovasc Dev Dis
 2 , 214 , 2015
Cells
Valve interstitial cells
Enzymes
Collagenase Type 2 : 600 u/ml
Medium
DMEM
Heart
Rat
Rat, SD, 1 day
Clark, A. and Naya, F.
MicroRNAs in the Myocyte Enhancer Factor 2 (MEF2)-regulated Gtl2-Dio3 Noncoding RNA Locus Promote Cardiomyocyte Proliferation by Targeting the Transcriptional Coactivator Cited2. ,
J Biol Chem
 290 , 23162-72 , 2015
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.025%
Medium
HBSS
Heart
Rat
Rat, Wistar, 200g
Miller, L. , Greensmith, D. , Sankaranarayanan, R. , O'Neill, S. and Eisner D.
The Effect of 2,5-di-(tert-butyl)-1,4-benzohydroquinone (TBQ) on Intracellular Ca2+ Handling in Rat Ventricular Myocytes. ,
Cell Calcium
 58 , 208-14 , 2015
Cells
Ventricular myocytes
Enzymes
Collagenase Type 2 : 95 u/ml
Protease : 0.1 u/ml
Medium
HEPES
Heart
Rat
Rat, SD, 3-4 month
Liu, L. , Wang, Y. , Cao, Z. , Wang, M. , Liu, X. , Gao, T. , Hu, Q. , Yuan, W. and Lin, L.
Up-regulated TLR4 in Cardiomyocytes Exacerbates Heart Failure after Long-Term Myocardial Infarction. ,
J Cell Mol Med
 19 , 2728 , 2015
Cells
Cardiomyocytes
Enzymes
Medium
DMEM
Heart
Rat
Rat, neonatal
Zhang, B. , Montgomery, M. , Davenport-Huyer, L. , Korolj, A. and Radisic, M.
Platform Technology for Scalable Assembly of Instantaneously Functional Mosaic Tissues. ,
Sci. Adv.
 1 , e1500423 , 2015
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.06%
Collagenase Type 2 : 220 u/ml
Medium
HBSS
Heart
Rat
Rat, SD, 1-2 day
Rutering, J. , Ilmer, M. , Recio, A. , Coleman, M. , Vykoukal, J. and Alt, E.
Improved Method for Isolation of Neonatal Rat Cardiomyocytes with Increased Yield of C-Kit+ Cardiac Progenitor Cells. ,
J Stem Cell Res Ther
 5 , 1-8 , 2015
Cells
Cardiomyocytes
Enzymes
Medium
DMEM/F-12
Heart
Rat
Rat, neonatal
Sakurai, T. , Lanahan, A. , Woolls, M. , Li, N. , Tirziu, D. and Murakami, M.
Live Cell Imaging of Primary Rat Neonatal Cardiomyocytes Following Adenoviral and Lentiviral Transduction using Confocal Spinning Disk Microscopy. ,
J Vis Exp
 , e51666 , 2014
Cells
Cardiomyocytes
Enzymes
NCIS kit : per instructions
Medium
L-15
Heart
Rat
Rat, adult, 300-370 g
Chouchani, E. , Pell, V. , Gaude, E. , Aksentijevic, D. , Sundier, S. , Robb, E. , Logan, A. , Nadtochiy, S. , Ord, E. , Smith, A. , Eyassu, F. , Shirley, R. , Hu, C. , Dare, A. , James, A. and Rogatti, S.
Ischaemic Accumulation of Succinate Controls Reperfusion Injury Through Mitochondrial ROS. ,
Nature
 515 , 431-435 , 2014
Cells
Cardiomyocytes
Enzymes
Medium
KH buffer
Heart
Rat
Rat
Hennessey, J. , Wei, E. and Pitt, G.
Fibroblast Growth Factor Homologous Factors Modulate Cardiac Calcium Channels. ,
Circ Res
 113 , 381-8 , 2013
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 150 u/ml
Medium
MEM
Heart
Rat
Rat, Wistar, 175-200g
Eckerle, L. , Felix, S. and Herda, L.
Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes. ,
J Vis Exp
 73 , e4237 , 2013
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 150 u/ml
Medium
see reference
Heart
Rat
Rat, SD, 1-2 day
Zhang, B. , Green, J. , Murthy, S. and Radisic, M.
Label-Free Enrichment of Functional Cardiomyocytes using Microfluidic Deterministic Lateral Flow Displacement. ,
PLoS ONE
 7 , e37619 , 2012
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.06%
Collagenase Type 2 : 220 u/ml
Medium
DMEM
Heart
Rat
Rat, adult
Karakikes, I. , Hadri, L. , Rapti, K. , Ladage, D. , Ishikawa, K. , Tilemann, L. , Yi, G. , Morel, C. , Gwathmey, J. , Zsebo, K. , Weber, T. , Kawase, Y. and Hajjar, R.
Concomitant Intravenous Nitroglycerin with Intracoronary Delivery of AAV1.SERCA2a Enhances Gene Transfer in Porcine Hearts. ,
Mol Ther
 20 , 565 , 2012
Cells
Ventricular myocytes
Enzymes
Medium
Medium 199

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.