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Tissue References Search Tool

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Eye
Rat

Rat, SD, 1-2 day

Lian, W. , Hu, X. , Zhang, J. , Wu, Y. , Zhao, N. , Ma, H. , He, H. and Lu, Q.
Fucoxanthin Protects Retinal Ganglion Cells and Promotes Parkin-mediated Mitophagy against Glutamate Excitotoxicity. ,
Neuroreport
 34 , 385-394 , 2023
Cells
Retinal ganglion
Enzymes
Papain : 0.5%
Medium
MEM
Eye
Rat
Adult, female, 170-200 g
Mead, B. and Tomarev, S.
Bone Marrow-Derived Mesenchymal Stem Cells-Derived Exosomes Promote Survival of Retinal Ganglion Cells Through miRNA-Dependent Mechanisms. ,
Stem Cells Transl Med
 6 , 1273-1285 , 2017
Cells
Retinal
Enzymes
PDS kit : with modifications
Medium
EBSS
Eye
Rat
Rat, Wister, 3-30 wk
Cameron, M. , Kekesi, O. , Morley, J. , Tapson, J. , Breen, P. , Van Schaik, A. and Buskila, Y.
Calcium Imaging of AM Dyes Following Prolonged Incubation in Acute Neuronal Tissue. ,
PLoS ONE
 11 , e0155468 , 2016
Cells
Retinal
Enzymes
Papain : 30 u/ml
Medium
EBSS
Eye
Rat
Rat, SD, 6-8 week
Vigneswara, V. , Esmaeili, M. , Deer, L. , Berry, M. , Logan, A. and Ahmed, Z.
Eye Drop Delivery of Pigment Epithelium-Derived Factor-34 Promotes Retinal Ganglion Cell Neuroprotection and Axon Regeneration. ,
Mol Cell Neurosci
 68 , 212-21 , 2015
Cells
Retinal
Enzymes
PDS kit : per instructions
Medium
EBSS
Eye
Rat
Rat, SD
Tan, C. , Andrews, M. , Kwok, J. , Heintz, T. , Gumy, L. , Fassler, R. and Fawcett, J.
Kindlin-1 Enhances Axon Growth on Inhibitory Chondroitin Sulfate Proteoglycans and Promotes Sensory Axon Regeneration. ,
J Neurosci
 32 , 7325 , 2012
Cells
Retinal ganglion
Enzymes
PDS kit : with modifications
Medium
Neurobasal
Eye
Rat
Rat, SD, 180-220g
Ma, J. , Yu, W. , Wang, Y. , Cao, G. , Cai, S. , Chen, X. , Yan, N. , Yuan, Y. , Zeng, H. , Fleenor, D. , Liu, X. and Pang, I.
Neuroprotective Effects of C-type Natriuretic Peptide on Rat Retinal Ganglion Cells. ,
Inv Ophthal Visual Sci
 51 , 3544-53 , 2010
Cells
Retinal ganglion
Enzymes
Papain : 0.2%
Medium
Neurobasal
Eye
Rat
Rat, Fisher, adult
Suzuki, T. , Mandai, M. , Akimoto, M. , Yoshimura, N. and Takahashi, M.
The Simultaneous Treatment of MMP-2 Stimulants in Retinal Transplantation Enhances Grafted Cell Migration into the Host Retina. ,
Stem Cells
 24 , 2406 , 2006
Cells
Retinal
Enzymes
PDS kit : per instructions
Medium
MEM
Eye
Rat
Rat, Dark Agouti, 3-4 week
Akagi T, Mandai M, Ooto S, Hirami Y, Osakada F, Kageyama R, Yoshimura N, Takahashi M.
Otx2 homeobox gene induces photoreceptor-specific phenotypes in cells derived from adult iris and ciliary tissue ,
Inv Ophthal Visual Sci
 45 , 4570 , 2004
Cells
Ciliary-derived eye
Enzymes
PDS kit : with modifications
Medium
DMEM/F12
Eye
Rat
Rat, embryonic 17 day
Seigel, G.
The Golden Age of Retinal Cell Culture ,
Mol Vis
 5 , 4 , 1999
Cells
Retinal
Enzymes
Trypsin : 0.6%
Medium
DMEM
Eye
Rat
Rat, SD
Jing, S. , Wen, D. , Yu, Y. , Holst, P. , Luo, Y. , Fang, M. , Tamir, R. , Antonio, L. , Hu, Z. and et al.
GDNF-Induced Activation of the Ret Protein Tyrosine Kinase is Mediated by GDNFR-a, a Novel Receptor for GDNF ,
Cell
 85 , 1113 , 1996
Cells
Retina
Enzymes
Papain : 120 U
Medium
HBSS, PBS
Eye
Rat
Rat, 4-14 week
Wang N, Koutz CA. and Anderson RE.
A method for the isolation of retinal pigment epithelial cells from adult rats ,
Inv Ophthal Visual Sci
 34(1) , 101 , 1993
Cells
Retinal pigment epithelial cells
Enzymes
Collagenase Type 1 : 65 u/ml
Hyaluronidase : 220 u/ml
Medium
CF Hanks with EDTA
Eye
Rat
Rat, 6-8 day
Chang CW, Roque RS, Defoe DM. and Caldwell RB.
An improved method for isolation and culture of pigment epithelial cells from rat retina ,
Curr Eye Res
 10(11) , 1081 , 1991
Cells
Retinal pigment epithelial
Enzymes
Medium
DMEM
Eye
Rat
Rat, Long Evans, 1-15 days
Huettner, J. and Baughman, R.
Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats ,
J Neurosci
 6 , 3044 , 1986
Cells
Neurons, visual cortex
Enzymes
Papain : 20 u/ml
Medium
BSS
(see reference)
Eye
Rat
Rat, SD, pups
Sarthy PV, Curtis BM. and Catterall WA.
Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat ,
J Neurosci
 3 (12) , 2532 , 1983
Cells
Retina
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Kidney
Rat
Rat, SD, fetal
Gupta, A. , Jadhav, S. , Tripathy, N. and Nityanand, S.
Fetal Kidney Cells Can Ameliorate Ischemic Acute Renal Failure in Rats through Their Anti-Inflammatory, Anti-Apoptotic and Anti-Oxidative Effects. ,
PLoS ONE
 10 , e0131057 , 2015
Cells
Fetal kidney
Enzymes
Medium
MEM
Kidney
Rat
Rat, Lewis, male
Basu, J. , Genheimer, C. , Sangha, N. , Quinlan, S. , Guthrie, K. , Kelley, R. , Ilagan, R. , Jain, D. , Bertram, T. and Ludlow, J.
Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. ,
Lipids Health Dis
 10 , 171 , 2011
Cells
Renal adipose derived cells
Enzymes
Medium
DMEM
Kidney
Rat
Rat
Panico, C. , Luo, Z. , Damiano, S. , Artigiano, F. , Gill, P. and Welch, W.
Renal Proximal Tubular Reabsorption is Reduced in Adult Spontaneously Hypertensive Rats: Roles of Superoxide and Na+/H+ Exchanger 3. ,
Hypertension
 54 , 1291 , 2009
Cells
Proximal tubule
Enzymes
Medium
see reference
Kidney
Rat
Rat, SD, male, 200-250g
Silva, G. , Beierwaltes, W. and Garvin, J.
Extracellular ATP stimulates NO production in rat thick ascending limb ,
Hypertension
 47 , 563-7 , 2006
Cells
Medullary thick ascending limb
Enzymes
Collagenase : 0.1%
Medium
HEPES-saline
Kidney
Rat
Rat, Wistar, 300g
Deng Aihua, Miracle Cynthia M, Lortie Mark, Satriano Joseph, Gabbai Francis B, Munger Karen A, Thomson Scott C, Blantz Roland C.
Kidney oxygen consumption, carbonic anhydrase, and proton secretion ,
Am J Physiol/Renal
 290 , F1009-15 , 2006
Cells
Renal proximal tubules
Enzymes
Medium
DMEM/F12
Kidney
Rat
Rat, Wistar, male, 300-350 g
Deng, A. , Miracle, C. , Suarez, J. , Lortie, M. , Satriano, J. , Thomson, S. , Munger, K. and Blantz, R.
Oxygen Consumption in the Kidney: Effects of Nitric Oxide Synthase Isoforms and Angiotensin II. ,
Kidney Int
 68 , 723 , 2005
Cells
Renal proximal tubules
Enzymes
Medium
DMEM/F-12
Kidney
Rat
Rat
Arystarkhova, E. , Wetzel R. and Sweadner K.
Distribution and Oligomeric Association of Splice Forms of Na(+)-K(+)-ATPase Regulatory Gamma-subunit in Rat Kidney ,
Am J Physiol/Renal
 282(3) , F393 , 2002
Cells
Cortical tubule cells
Enzymes
Collagenase : 0.6%
Medium
DMEM
Kidney
Rat
Rat, SD, male 275-300 g
Amiri Farhad, Shaw Sean, Wang Xiaodan, Tang Jie, Waller Jennifer L, Eaton Douglas C, Marrero Mario B.
Angiotensin II activation of the JAK/STAT pathway in mesangial cells is altered by high glucose ,
Kidney Int Suppl
 61 , 1605-16 , 2002
Cells
Glomerular mesangial cells
Enzymes
Collagenase Type 1 : 250 u/ml
Medium
DMEM
Kidney
Rat
Rat, SD, 1 week
Valencia L. , Bidet M. , Martial S. , Sanchez E. , Melendez E. , Tauc M. , Poujeol C. , Martin D. , Namorado M. , Reyes J. and Poujeol P.
Nifedipine-activated Ca(2+) Permeability in Newborn Rat Cortical Collecting Duct Cells in Primary Culture ,
Am J Physiol Cell Physiol
 280(5) , C1193 , 2001
Cells
Cortical collection duct
Enzymes
Medium
DMEM/Ham's F12
Kidney
Rat
Rat, SD, 250-300 g
Li, N. , Teggatz, E. , Li, P. , Allaire, R. and Zou, A.
Formation and Actions of Cyclic ADP-Ribose in Renal Microvessels ,
Microvasc Res
 60 , 149 , 2000
Cells
Microvessels
Enzymes
Medium
PSS
Kidney
Rat
Rat, SD, 250-300 g
Mattson D. and Wu F.
Nitric Oxide Synthase Activity and Isoforms in Rat Renal Vasculature ,
Hypertension
 35 , 337 , 2000
Cells
Renal
Enzymes
Medium
see reference
Kidney
Rat
Rat, SD, male, 5-6 week
Miyata, N. , Park, F. , Li, X. and Cowley, A.
Distribution of Angiotensin AT1 and AT2 Receptor Subtypes in the Rat Kidney ,
Am J Physiol
 277 , F437 , 1999
Cells
Renal tubules
Enzymes
Collagenase : 0.1%
Medium
see reference
Kidney
Rat
Rat, SD, 225-250 g
Amiri F, Garcia R.
Regulation of angiotensin II receptors and PKC isoforms by glucose in rat mesangial cells ,
Am J Physiol
 276 , F691-9 , 1999
Cells
Glomerular mesangial cells
Enzymes
Collagenase Type 1 : 250 u/ml
Medium
DMEM
Kidney
Rat
Rat, SD, male
Eitle E, Hiranyachattada S, Wang H, Harris PJ.
Inhibition of proximal tubular fluid absorption by nitric oxide and atrial natriuretic peptide in rat kidney ,
Am J Physiol
 274 , C1075-80 , 1998
Cells
Proximal tubule suspensions
Enzymes
Pronase E : 2 u/ml
Medium
HEPES buffer
Kidney
Rat
Rat, SD, male
Schafer, J. , Watkins, M. , Li, L. , Herter, P. , Haxelmans, S. and Schlatter, E.
A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments ,
Am J Physiol
 273 , F650 , 1997
Cells
Nephron segments
Enzymes
Medium
Eagle's MEM
Kidney
Rat
Rat, 12-weeks-old
Ishikawa, S. , Kusaka, I. , Higashiyama, M. , Nagasaka, S. , Saito, T. , Honda, K. and Saito, T.
Cellular Signaling and Proliferative Action of AVP in Mesangium of SHR: Effect of Low Density Lipoprotein ,
Nat Neurosci
 50 , 1506 , 1996
Cells
Renal
Enzymes
Collagenase : 0.1%
Medium
DMEM
Kidney
Rat
Rat, male, 150-200 g
Elliget, K. and Trump, B.
Primary Cultures of Normal Rat Kidney Proximal Tubule Epithelial Cells for Studies of Renal Cell Injury ,
In Vitro Cell Dev Biol
 27 , 739 , 1991
Cells
Epithelial
Proximal tubule
Enzymes
Protease : 0.1%
Medium
HBSS/CMF
Kidney
Rat
Rat, adult
Brion, L. , Schwartz, J. , Lachman, H. , Zavilowitz, B. and Schwartz, G.
Development of H+ Secretion by Cultured Renal Inner Medullary Collecting Duct Cells ,
Am J Physiol
 257 , F486 , 1989
Cells
Inner medullary collecting duct
Papillae
Enzymes
Medium
PBS
Kidney
Rat
Rat, Wistar, female, 100-150 g
Wang, J. , Kester, M. and Dunn, M.
The Effects of Endotoxin on Platelet-Activating Factor Synthesis in Cultured Rat Glomerular Mesangila Cells ,
Biochim Biophys Acta
 969 , 217 , 1988
Cells
Glomerular mesangial
Enzymes
Collagenase : 0.025%
Medium
RPMI 1640
Kidney
Rat
Rat, Wistar, male, 150 - 200 g
Barlet-Bas, C. , Khadouri, C. , Marsey, S. and Doucet, A.
Sodium-Independent In Vitro Induction of Na+,K+-ATPase by Aldosterone in Renal Target Cells: Permissive Effect of Triiodothyronine ,
Proc Natl Acad Sci U S A
 85 , 1707 , 1988
Cells
Renal target
Enzymes
Collagenase : 1.0% (also 0.7%)
Medium
Eagle's MEM
Kidney
Rat
Rat, Wistar, male, adult
Gesek, F. , Wolff, D. and Strandhov, J.
Improved Separation Method for Rat Proximal and Distal Renal Tubules ,
Am J Physiol
 253 , F358 , 1987
Cells
Proximal tubules
Enzymes
Collagenase : 0.2%
Medium
Krebs-Henseleit buffer
Kidney
Rat
Rat,Wistar, 300-400 gm
Vinay, P. , Gougoux, A. and Lemieux, G.
Isolation of a Pure Suspension of Rat Proximal Tubules ,
Am J Physiol
 241 , F403 , 1981
Cells
Proximal tubules
Enzymes
Collagenase : 0.15 %
Medium
Krebs Henseleit solution

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.