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Tissue References Search Tool

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Eye
Rat

Rat, SD, 1-2 day

Lian, W. , Hu, X. , Zhang, J. , Wu, Y. , Zhao, N. , Ma, H. , He, H. and Lu, Q.
Fucoxanthin Protects Retinal Ganglion Cells and Promotes Parkin-mediated Mitophagy against Glutamate Excitotoxicity. ,
Neuroreport
 34 , 385-394 , 2023
Cells
Retinal ganglion
Enzymes
Papain : 0.5%
Medium
MEM
Eye
Rat
Adult, female, 170-200 g
Mead, B. and Tomarev, S.
Bone Marrow-Derived Mesenchymal Stem Cells-Derived Exosomes Promote Survival of Retinal Ganglion Cells Through miRNA-Dependent Mechanisms. ,
Stem Cells Transl Med
 6 , 1273-1285 , 2017
Cells
Retinal
Enzymes
PDS kit : with modifications
Medium
EBSS
Eye
Rat
Rat, Wister, 3-30 wk
Cameron, M. , Kekesi, O. , Morley, J. , Tapson, J. , Breen, P. , Van Schaik, A. and Buskila, Y.
Calcium Imaging of AM Dyes Following Prolonged Incubation in Acute Neuronal Tissue. ,
PLoS ONE
 11 , e0155468 , 2016
Cells
Retinal
Enzymes
Papain : 30 u/ml
Medium
EBSS
Eye
Rat
Rat, SD, 6-8 week
Vigneswara, V. , Esmaeili, M. , Deer, L. , Berry, M. , Logan, A. and Ahmed, Z.
Eye Drop Delivery of Pigment Epithelium-Derived Factor-34 Promotes Retinal Ganglion Cell Neuroprotection and Axon Regeneration. ,
Mol Cell Neurosci
 68 , 212-21 , 2015
Cells
Retinal
Enzymes
PDS kit : per instructions
Medium
EBSS
Eye
Rat
Rat, SD
Tan, C. , Andrews, M. , Kwok, J. , Heintz, T. , Gumy, L. , Fassler, R. and Fawcett, J.
Kindlin-1 Enhances Axon Growth on Inhibitory Chondroitin Sulfate Proteoglycans and Promotes Sensory Axon Regeneration. ,
J Neurosci
 32 , 7325 , 2012
Cells
Retinal ganglion
Enzymes
PDS kit : with modifications
Medium
Neurobasal
Eye
Rat
Rat, SD, 180-220g
Ma, J. , Yu, W. , Wang, Y. , Cao, G. , Cai, S. , Chen, X. , Yan, N. , Yuan, Y. , Zeng, H. , Fleenor, D. , Liu, X. and Pang, I.
Neuroprotective Effects of C-type Natriuretic Peptide on Rat Retinal Ganglion Cells. ,
Inv Ophthal Visual Sci
 51 , 3544-53 , 2010
Cells
Retinal ganglion
Enzymes
Papain : 0.2%
Medium
Neurobasal
Eye
Rat
Rat, Fisher, adult
Suzuki, T. , Mandai, M. , Akimoto, M. , Yoshimura, N. and Takahashi, M.
The Simultaneous Treatment of MMP-2 Stimulants in Retinal Transplantation Enhances Grafted Cell Migration into the Host Retina. ,
Stem Cells
 24 , 2406 , 2006
Cells
Retinal
Enzymes
PDS kit : per instructions
Medium
MEM
Eye
Rat
Rat, Dark Agouti, 3-4 week
Akagi T, Mandai M, Ooto S, Hirami Y, Osakada F, Kageyama R, Yoshimura N, Takahashi M.
Otx2 homeobox gene induces photoreceptor-specific phenotypes in cells derived from adult iris and ciliary tissue ,
Inv Ophthal Visual Sci
 45 , 4570 , 2004
Cells
Ciliary-derived eye
Enzymes
PDS kit : with modifications
Medium
DMEM/F12
Eye
Rat
Rat, embryonic 17 day
Seigel, G.
The Golden Age of Retinal Cell Culture ,
Mol Vis
 5 , 4 , 1999
Cells
Retinal
Enzymes
Trypsin : 0.6%
Medium
DMEM
Eye
Rat
Rat, SD
Jing, S. , Wen, D. , Yu, Y. , Holst, P. , Luo, Y. , Fang, M. , Tamir, R. , Antonio, L. , Hu, Z. and et al.
GDNF-Induced Activation of the Ret Protein Tyrosine Kinase is Mediated by GDNFR-a, a Novel Receptor for GDNF ,
Cell
 85 , 1113 , 1996
Cells
Retina
Enzymes
Papain : 120 U
Medium
HBSS, PBS
Eye
Rat
Rat, 4-14 week
Wang N, Koutz CA. and Anderson RE.
A method for the isolation of retinal pigment epithelial cells from adult rats ,
Inv Ophthal Visual Sci
 34(1) , 101 , 1993
Cells
Retinal pigment epithelial cells
Enzymes
Collagenase Type 1 : 65 u/ml
Hyaluronidase : 220 u/ml
Medium
CF Hanks with EDTA
Eye
Rat
Rat, 6-8 day
Chang CW, Roque RS, Defoe DM. and Caldwell RB.
An improved method for isolation and culture of pigment epithelial cells from rat retina ,
Curr Eye Res
 10(11) , 1081 , 1991
Cells
Retinal pigment epithelial
Enzymes
Medium
DMEM
Eye
Rat
Rat, Long Evans, 1-15 days
Huettner, J. and Baughman, R.
Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats ,
J Neurosci
 6 , 3044 , 1986
Cells
Neurons, visual cortex
Enzymes
Papain : 20 u/ml
Medium
BSS
(see reference)
Eye
Rat
Rat, SD, pups
Sarthy PV, Curtis BM. and Catterall WA.
Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat ,
J Neurosci
 3 (12) , 2532 , 1983
Cells
Retina
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Tumor
Rat
Rat, adult
Duarte, S. , Momier, D. , Baque, P. , Casanova, V. , Loubat, A. , Samson, M. , Guigonis, J. , Staccini, P. , Saint-Paul, M. , De Lima, M. , Carle, G. and Pierrefite-Carle, V.
Preventive Cancer Stem Cell-Based Vaccination Reduces Liver Metastasis Development in a Rat Colon Carcinoma Syngeneic Model. ,
Stem Cells
 31 , 423-32 , 2013
Cells
Tumor
Enzymes
Medium
DMEM/F12
Tumor
Rat
Rat
Mehta, N. , Lopez, P. , Vyas, A. and Schnaar, R.
Gangliosides and Nogo Receptors Independently Mediate Myelin-Associated Glycoprotein Inhibition of Neurite Outgrowth in Different Nerve Cells. ,
J Biol Chem
 282 , 27875-86 , 2007
Cells
Hippocampal neurons
Enzymes
Papain : 0.2%
Medium
Hibernate A
Tumor
Rat
Rat, male 12 week
Sharma N, Luo J, Kirschmann DA, O'Malley Y, Robbins ME, Akporiaye ET, Lubaroff DM, Heidger PM, Hendrix MJ.
A novel immunological model for the study of prostate cancer ,
Cancer Res
 59 , 2271-6 , 1999
Cells
Sponge infiltrating cells
Enzymes
Medium
RPMI 1640
Tumor
Rat
Rat (ACI/NMs X BUF/Mna) F1, male, 28 months, rat, (ACI/MNs) male, 8 weeks
Masuda, A. , Ohtsuka, K. and Matsuyama, M.
Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus ,
In Vitro Cell Dev Biol
 26 , 713 , 1990
Cells
Epthelial
Enzymes
Medium
Eagle's MEM Serum-free
Tumor
Rat
Rat
Brennan, M. , Oldberg, A. , Hayman, E. and Ruoslahti, E.
Effect of a Proteoglycan Produced by Rat Tumor Cells on Their Adhesion to Fibronectin-Collagen Substrata ,
Cancer Res
 43 , 4302 , 1983
Cells
Yolk sac tumor
Enzymes
Trypsin : 0.01%
Medium
DMEM
Tumor
Rat
Rat, SD, female
Cohen, L.
Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma ,
In Vitro
 18 , 565 , 1982
Cells
Epithelial, cancer and tumor
Enzymes
Collagenase : 0.1%
Medium
Eagles's MEM
Tumor
Rat
Rat
Gazdar, A. , Chick, W. , Oie, H. , Sims, H. , King, D. , Weir, G. and Lauris, V.
Continuous, Clonal, Insulin-and Somatostatin-Secreting Cell Lines Established from a Transplantable Rat Islet Cell Tumor ,
Proc Natl Acad Sci U S A
 77 (6) , 3519 , 1980
Cells
Tumor, islet
Enzymes
Trypsin : 0.05%
Medium
Medium 199
Tumor
Rat
Rat
Essner, E.
Experiments on an Ascites Hepatoma. I. Enzymatic Digestion and Alkaline Degradation of the Cementing Substance and Separation of Cells, in Tumor Islands ,
Exp Cell Res
 7 , 430 , 1954
Cells
Ascites hepatoma
Enzymes
Trypsin : 0.1%
Medium
Phosphate buffer (see reference)

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.