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Tissue References Search Tool

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Muscle
Rat
Rat, day 7
Vilmont, V. , Cadot, B. , Ouanoumou, G. and Gomes, E.
A System for Studying Mechanisms of Neuromuscular Junction Development and Maintence ,
Development
 143 , 2464 , 2016
Cells
Skeletal muscle
Enzymes
Collagenase : 0.05%
Medium
DMEM
Muscle
Rat
Rat, SD
Zeng, Y. , Liu, H. , Kang, K. , Wang, Z. , Hui, G. , Zhang, X. , Zhong, J. , Peng, W. , Ramchandran, R. , Raj, J. and Gou, D.
Hypoxia Inducible Factor-1 Mediates Expression of miR-322: Potential Role in Proliferation and Migration of Pulmonary Arterial Smooth Muscle Cells. ,
Sci Rep
 5 , 12098 , 2015
Cells
Pulmonary arterial smooth muscle
Enzymes
Elastase : 0.05%
Medium
Buffer
Muscle
Rat
Rat
Weisleder, N. , Zhou, J. and Ma, J.
Detection of Calcium Sparks in Intact and Permeabilized Skeletal Muscle Fibers. ,
Methods Mol Biol
 798 , 395 , 2012
Cells
Skeletal muscle fiber
Enzymes
Medium
Tyrode
Muscle
Rat
Rat
Weber, S. , Gratopp, A. , Akanbi, S. , Rheinlaender, C. , Sallmon, H. , Barikbin, P. and Koehne, P.
Isolation and Culture of Fibroblasts, Vascular Smooth Muscle, and Endothelial Cells From the Fetal Rat Ductus Arteriosus ,
Pediatr Res
 70 , 236 , 2011
Cells
Vascular smooth muscle
Enzymes
Medium
DMEM
Muscle
Rat
Rat, fetal 17-18 day
Das, M. , Rumsey, J. , Bhargava, N. , Stancescu, M. and Hickman, J.
Skeletal Muscle Tissue Engineering: A Maturation Model Promoting Long-Term Survival of Myotubes, Structural Development of the Excitation-Contraction Coupling Apparatus and Neonatal Myosin Heavy Chain Expression. ,
Biomaterials
 30 , 5392 , 2009
Cells
Myotubes
Enzymes
Trypsin : 0.05%
Medium
see reference
Muscle
Rat
Rat
Pacak, C. and Cowan, D.
Fabrication of Myogenic Engineered Tissue Constructs. ,
J Vis Exp
 27 , e1137 , 2009
Cells
Myogenic
Enzymes
Medium
Ham's F-10
Muscle
Rat
Rat
Larkin, L. , Calve, S. , Kostrominova, T. and Arruda, E.
Structure and Functional Evaluation of Tendon-Skeletal Muscle Constructs Engineered In Vitro. ,
Tiss Eng
 12 , 3149-58 , 2006
Cells
myogenic
Enzymes
Neutral Protease : 8 u/ml
Collagenase Type 4 : 200 u/ml
Medium
DMEM
Muscle
Rat
Rat, Lewis, neonatal
Kim, J. , Hadlock, T. , Cheney, M. , Varvares, M. and Marler, J.
Muscle Tissue Engineering for Partial Glossectomy Defects. ,
Arch Facial Plast Surg
 5 , 403 , 2003
Cells
Myoblasts
Enzymes
Medium
Ham's F-10
Muscle
Rat
Rat, SD, adult and neonatal w/in 1 day of birth
Dennis, R. , Kosnik II, P. , Gilbert, M. and Faulkner, J.
Excitability and Contractility of Skeletal Muscle Engineered from Primary Cultures and Cell Lines ,
Am J Physiol Cell Physiol
 280 , C288 , 2001
Cells
Myooids
Enzymes
Neutral Protease : 4 u/ml
Medium
Ham's F-12
Muscle
Rat
Rat, SD, 250 g
Jaggar JH.
Intravascular pressure regulates local and global Ca(2+) signaling in cerebral artery smooth muscle cells ,
Am J Physiol Cell Physiol
 281 , C439-48 , 2001
Cells
Arterial smooth muscle
Enzymes
Papain : 0.03%
Collagenase : 0.1%
Medium
see reference
Muscle
Rat
Rat, SD, 3 month
Stevenson S. , Rollence M. , Marshall-Neff J. and Liau G.
A Genetically Modified Adenoviral Vector Exhibits Enhanced Gene Transfer of Human Smooth Muscle Cells ,
J Vasc Res
 38(5) , 471 , 2001
Cells
Smooth muscle cells
Enzymes
Medium
M-199
Muscle
Rat
Rat, Wistar, adult, male
Wellman, G. , Barrett-Jolley, R. , Koppel, H. Everitt, D. and Quayle, J.
Inhibition of Vascular KATP Channels by U-37883A: A Comparison with Cardiac and Skeletal Muscle ,
Br J Pharmacol
 128 , 909 , 1999
Cells
Smooth & skeletal muscle
Cardiac myocytes
Enzymes
Protease : 0.01%
Medium
PSS
Muscle
Rat
Rat, SD, male, 250-350 g
Hrometz, S. , Edelmann, S. , McCune, D. , Olges, J. , Hadley, R. , Perez ,D. and Piascik, M.
Expression of Multiple Alpha1-Adrenoceptors on Vascular Smooth Muscle: Correlation with the Regulation of Contraction ,
J Pharmacol Exp Ther
 290(1) , 452 , 1999
Cells
Vascular smooth muscle
Enzymes
Elastase : 0.0125%
Medium
DMEM
Muscle
Rat
Rat, SD, male, 150-175 g
Redmond, E. , Cahill, P. and Sitzmann, J.
Perfused Transcapillary Smooth Muscle and Endothelial Cell Co-Culture-A Novel In Vitro Model ,
In Vitro Cell Dev Biol Anim
 31 , 601 , 1995
Cells
Smooth muscle, endothelial
Enzymes
Trypsin : 0.04%
Medium
MEM
Muscle
Rat
Rat, Wistar Kyoto, 10-15 weeks
McGuire, P. , Walker-Caprioglio, H. , Little, S. and McGuffee, L.
Isolation and Culture of Rat Superior Mesenteric Artery Smooth Muscle Cells ,
In Vitro Cell Dev Biol
 29 , 135 , 1993
Cells
Smooth muscle, mesenteric artery
Enzymes
Trypsin : 0.05%
Medium
MEM
Muscle
Rat
Rat
(also rabbit, guinea-pig)
Hu, S. and Kim, H.
Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid ,
FASEB J
 6 , A383 , 1992
Cells
Smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Potassium buffer solution
Muscle
Rat
Rat, SD, 19 days
Loch-Caruso, R. , Pahl, M. and Juberg, D.
Rat Myometrial Smooth Muscle Cells Show High Levels of Gap Junctional Communication Under a Variety of Culture Conditions ,
In Vitro Cell Dev Biol
 28 , 97 , 1992
Cells
Smooth muscle, myometrial
Enzymes
Trypsin : 150 µg/ml
Medium
HBSS or PSS, CMF
Muscle
Rat
Rat, SHRs and WKY, male, 10 - 14 weeks
Bolzon, B. and Cheung, D.
Isolation and Characterization of Single Vascular Smooth Muscle Cells From Spontaneously Hypertensive Rats ,
Hypertension
 14 , 137 , 1989
Cells
Smooth muscle, tail arteries
Enzymes
Papain : 0.1%
Medium
HEPES buffer
(see reference)
Muscle
Rat
Rat, 1-3 day
Barone, L. , Wolfe, L. , Faris, B. and Franzblau, C.
Elastin mRNA Levels and Insoluble Elastin Accumulation in Neonatal Rat ,
Biochemistry
 27 , 3175 , 1988
Cells
Smooth muscle, aortic
Enzymes
Elastase : 0.0125%
Medium
DMEM
Muscle
Rat
Rat
Bean, B. , Sturek, M. , Puga, A. and Hermsmeyer, K.
Calcium Channels in Muscle Cells Isolated From Rat Mesenteric Arteries: Modulation by Dihydropyridine Drugs ,
Circ Res
 59 , 229 , 1986
Cells
Muscle, mesenteric arteries
Enzymes
Trypsin : 0.05%
Medium
HEPES KG solution
(see reference)
Muscle
Rat
Rat, SHR, WKY. either sex, 12-19 day, 3 month, and retired breeders
Gordon, D. , Mohai, L. and Schwartz, S.
Induction of Polyploidy in Cultures of Neonatal Rat Aortic Smooth Muscle Cells ,
Circ Res
 59 , 633 , 1986
Cells
Endothelial, aortic
Enzymes
Elastase : 0.05%
Medium
Waymouth's culture medium
Muscle
Rat
Rat, male, 150-250 g
Cole, O. , Fan, T. and Lewis, G.
Isolation, Characterization, Growth and Culture of Endothelial Cells From the Rat Aorta ,
Cell Biol Int Rep
 10 (6) , 399 , 1986
Cells
Endothelial, aortic
Medium
RPMI 1640
Muscle
Rat
Rat, SD, male, 200 - 250 g
Brock, T. , Alexander, R. , Ekstein, L. , Atkinson, W. and Gimbrone, M.
Angiotensin Increases Cytosolic Free Calcium in Cultured Vascular Smooth Muscle Cells ,
Hypertension
 7 , 105 , 1985
Cells
Smooth muscle, thoracic aorta
Enzymes
Trypsin : 0.0375%
Medium
Eagle's MEM with calcium
Muscle
Rat
Rat, Wistar, female, 10 weeks
Boulanger-Saunier, C. , Kattenburg, D. and Stoclet, J.
Cyclic AMP-dependent Phosphorylation of a 16kDa Protein in a Plasma Membrane-enriched Fraction of Rat Aortic Myocytes ,
F.E.B.S. Lett.
 193 , 283 , 1985
Cells
Myocytes
Enzymes
Collagenase : 0.1%
Medium
HBSS
Muscle
Rat
Rat, SD, 225-250g
Gunther S, Alexander RW, Atkinson WJ. and Gimbrone MA Jr.
Functional angiotensin II receptors in cultured vascular smooth muscle cells ,
J Cell Biol
 92 , 289 , 1982
Cells
Mesenteric artery smooth muscle cells
Enzymes
Medium
HBSS
Muscle
Rat
Rat, 3-4 day
Kasten, F.
Rat Myocardial Cells In Vitro: Mitosis and Differentiated Properties ,
In Vitro
 8 , 128 , 1972
Cells
Myocardial
Enzymes
Trypsin NF 1:250 : 0.125%
Medium
HBSS CMF
Muscle
Rat
Rat, 200 g
Kono, T.
Roles of Collagenases and Other Proteolytic Enzymes in the Dispersal of Animal Tissues ,
Biochim Biophys Acta
 178 , 397 , 1969
Cells
Muscle
Enzymes
Trypsin : 0.05%
Medium
Kreb's Henseleit bicarbonate buffer
Muscle
Rat
Rat, Wistar, 3-10 day
Harary, I. and Farley, B.
In Vitro Studies on Single Beating Rat Heart Cells ,
Exp Cell Res
 29 , 451 , 1963
Cells
Heart
Enzymes
Trypsin NF 1:250 : 250: 0.1%
Medium
Saline A
(See reference)
Reproductive
Rat
Rat
Jiang, H. , Zhang, Y. , Tian, K. , Wang, B. and Han, S.
Amelioration of Experimental Autoimmune Encephalomyelitis Through Transplantation of Placental Derived Mesenchymal Stem Cells. ,
Sci Rep
 7 , 41837 , 2017
Cells
Placental mesenchymal
Enzymes
Medium
DMEM
Reproductive
Rat
Rat, SD, 350-450 g
Sharma RS, Pal PC, Rajalakshmi M.
Isolation and Culture of Leydig Cells from Adult Rats ,
Indian J Clinical Biochem
 21 , 27 , 2006
Cells
Leydig cells
Enzymes
Collagenase : .05-0.1%
Medium
Medium 199
Reproductive
Rat
Rat, SD, adult, male, 8 - 10 week
Abou-Haila, A. and Tulsiani, D.
Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization ,
Biol Proced Online
 3 (1) , 35 , 2001
Cells
Seminiferous tubules
Enzymes
Trypsin : 0.05%
Medium
Krebs-Ringer bicarbonate buffer
(see reference)
Reproductive
Rat
Rat, SD, immature
Ando, M. , Kol, S. , Irahara, M. , Sirois, J. and Adashi, E.
Non-Steroidal Anti-Inflammatory Drugs (NSAIDS) Block the Late, Prostanoid-Dependent/Ceramide-Independent Component of Ovarian IL-1 Action: Implications for the Ovulatory Process ,
Mol Cell Endocrinol
 157 , 21 , 1999
Cells
Ovary
Enzymes
Collagenase Type 1 : 144 u/ml
Medium
McCoy's 5a
Reproductive
Rat
Rat, Han-Wistar, adult, male, 250 g
Leckie, C. , Welberg, L. and Seckl, J.
11B-Hydroxysteroid Dehydrogenase is a Predominant Reductase in Intact Rat Leydig Cells ,
J Endocrinol
 159 , 233 , 1998
Cells
Testes
Enzymes
Trypsin : 0.1%
Medium
Medium 199 w/ Hank's salts
Reproductive
Rat
Rat, SD, female, immature, 26 day
(also rat, pregnant and pseudopregnant)
Tellieria, C. , Ou, J. , Sugino, N. , Ferguson, S. and Gibori, G.
The Expression of Interleukin-6 in the Pregnant Rat Corpus Luteum and Its Regulation by Progesterone and Glucocorticoid ,
Endocrinology
 139 (8) , 3597 , 1998
Cells
Corpus luteum
Enzymes
Neutral Protease : 2.4 u/ml Deoxyribonuclease: 200u/ml
Medium
Serum-free medium
(see reference)
Reproductive
Rat
Rat, SD, 25 day
Duleba AJ, Spaczynski RZ, Olive DL, Behrman HR.
Effects of insulin and insulin-like growth factors on proliferation of rat ovarian theca-interstitial cells ,
Biol Reprod
 56 , 891-7 , 1997
Cells
Ovarian theca-interstitial
Enzymes
Medium
Medium 199
Reproductive
Rat
Rat, SD, male, 90 days
Onoda, M. and Djakiew, D.
Pachytene Spermatocyte Protein(s) Stimulate Sertoli Cells Grown in Bicameral Chambers: Dose-Dependent Secretion of Ceruloplasmin, Sulfated Glycoprotein-1, Sulfated Glycoprotein-2, and Transferrin ,
In Vitro Cell Dev Biol
 27 , 215 , 1991
Cells
Sertoli
Enzymes
Hyaluronidase : 0.1%
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.