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Tissue References Search Tool

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Brain
Insect
Drosophilia
Hadzic, T. , Park, D. , Abruzzi, K. , Yang, L. , Trigg, J. , Rohs, R. , Rosbash, M. and Taghert, P.
Genome-Wide Features of Neuroendocrine Regulation in Drosophila by the Basic Helix-Loop-Helix Transcription Factor DIMMED. ,
Nucleic Acids Res
 43 , 2199-215 , 2015
Cells
Neurons
Enzymes
Papain : 50 u/ml
Medium
Modified Dissecting Saline
Brain
Insect
Drosophila
Gu, H. and O'Dowd, D.
Whole Cell Recordings from Brain of Adult Drosophila. ,
J Vis Exp
 6 , 248 , 2007
Cells
Neurons
Enzymes
Papain : 20 u/ml
Medium
Saline
Brain
Insect
Drosophila
Sicaeros, B. Campusano, J. and O'Dowd, D.
Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae. ,
J Vis Exp
 4 , 200 , 2007
Cells
Neurons
Enzymes
Papain : 4 u/ml
Medium
DMEM
Brain
Insect
Drosophilia
Gu, H. and O'Dowd, D.
Cholinergic Synaptic Transmission in Adult Drosophila Kenyon Cells in Situ. ,
J Neurosci
 26 , 265-72 , 2006
Cells
Kenyon
Enzymes
Papain : 20 u/ml
Medium
Recording saline
Brain
Shellfish
Helisoma trivolvis
Hadley, R.D. , Bodnar, D.A. and Kater, S.B.
Formation of Electrical Synapses Between Isolated, Cultured Helisoma Neurons Requires Mutual Neurite Elongation ,
J Neurosci
 5 , 3145 , 1985
Cells
Neurons
Buccal ganglia
Enzymes
Trypsin : 0.2%
Medium
Saline, Sterile
Eye
Salamander
Salamander
Clarke, R, Wang, J. and Townes-Anderson, E.
Using Laser Tweezers For Manipulating Isolated Neurons In Vitro. ,
J Vis Exp
 19 , 911 , 2008
Cells
Neurons
Enzymes
Papain : 14 u/ml
Medium
Ringers
Eye
Salamander
Salamander, 18-25 cm
Townes-Anderson, E. , MacLeish, P. and Raviola, E.
Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase ,
J Cell Biol
 100 , 175 , 1985
Cells
Retina
Enzymes
Papain : 14 u/ml
Medium
Saline
Eye
Salamander
Salamander (A. tigrinum)
Bader, C. , MacLeish, P. and Schwartz, E.
Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina ,
Proc Natl Acad Sci U S A
 75 , 3507 , 1978
Cells
Photoreceptors, retina
Enzymes
Papain : 0.05%
Medium
(see reference)
Heart
Shellfish
Pacific Oysters
Le Duff, R. , Lipart, C. and Renault, T.
Primary Culture of Pacific Oyster, Crassostrea gigas, heart cells ,
J Tiss Cul Meth
 16 , 67 , 1994
Cells
Haemocytes
Medium
L15 medium
Heart
Shellfish
Clams, 3.0 to 4.5 cm
Wen, C. , Kou, G. and Chen, S.
Cultivation of Cells From the Heart of the Hard Clam, Merretrix lusoria (Roding) ,
J Tiss Cul Meth
 15 , 123 , 1993
Cells
Myocytes
Fibroblasts
Enzymes
Medium
L15 medium
Miscellaneous
Insect
Insect, Lepidoptera
Goodwin, R. and McCawley, P
Initiating Attached Cell Lines From the Lepidoptera (Insecta) ,
Meth Cell Sci
 3 , 567 , 1977
Cells
Lepitopteran
Enzymes
Medium
Dulbecco PBS
Neural
Insect
Drosophila, larval
Berger, C. , Harzer, H. , Burkard, T. , Steinmann, J. , van der Horst, S. , Laurenson, A. , Novatchkova, M. , Reichert, H. and Knoblich, J.
FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal. ,
Cell Rep
 2 , 407 , 2012
Cells
Neuroblasts
Enzymes
Papain : 0.1%
Medium
Rinaldini solution
Neural
Insect
Drosophila
Sanchez-Soriano, N. , Bottenberg, W. , Fiala, A. , Haessler, U. , Kerassoviti, A. , Knust, E. , Lohr, R. and Prokop, A.
Are Dendrites in Drosophila Homologous to Vertebrate Dendrites? ,
Dev Biol
 288 , 126 , 2005
Cells
Dendrites
Enzymes
Collagenase : 0.05%
Medium
HBSS
Neural
Insect
Gryllus Bimaculatus
Kloppenburg, P. and Horner, M.
Voltage-Activated Currents in Identified Giant Interneurons Isolated from Adult Crickets Gryllus Bimaculatus ,
J Exp Biol
 201 (Pt 17) , 2529 , 1998
Cells
Giant interneurons
Enzymes
Collagenase : 0.05%
Medium
Leibovitz's L15
Neural
Salamander
Salamander, 18-25 cm
Townes-Anderson, E. , MacLeish, P. and Raviola, E.
Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase ,
J Cell Biol
 100 , 175 , 1985
Cells
Retina
Enzymes
Papain : 14 u/ml
Medium
Saline
Neural
Salamander
Salamander (A.tigrinum)
Bader, C. , MacLeish, P. and Schwartz, E.
Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina ,
Proc Natl Acad Sci U S A
 75 , 3507 , 1978
Cells
Photoreceptors, retina
Enzymes
Papain : 0.05%
Medium
(see reference)
Neural
Shellfish
Aplysia californica
Lee, A. , Decourt, B. and Suter, D.
Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones. ,
J Vis Exp
 12 , 662 , 2008
Cells
Neurons
Enzymes
Medium
L-15-ASW
Neural
Shellfish
Snails (Helisoma trivolis), albino, adult
Zoran, M. , Doyle, R. and Haydon, P.
Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis ,
Neuron
 6 , 145 , 1991
Cells
Buccal ganglia; SLT muscle
Enzymes
Trypsin : 0.2%
Medium
DMEM
Neural
Shellfish
Pond snail (Helisoma) albino, adult
Haydon, P.
The Formation of Chemical Synapses Between Cell-Cultured Neuronal Somata ,
J Neurosci
 8 , 1032 , 1988
Cells
Somata, buccal ganglia
Enzymes
Trypsin : 0.2%
Medium
Antibiotic saline, Leibowitz 50%
Neural
Shellfish
Helisoma trivovlis
Cohan, C. , Haydon, P. and Kater, S.
Single Channel Activity Differs in Growing and Nongrowing Growth Cones of Isolated Identified Neurons of Helisoma ,
J Neurosci Res
 13 , 285 , 1985
Cells
Buccal ganglia
Enzymes
Trypsin : 0.2%
Medium
L-15 medium
Neural
Shellfish
Mollusc, juvenile 1-2 gm or adult 50-100 gm
Schacher, S. and Proshanksy, E.
Neurite Regeneration by Aplysia Neurons in Dissociated Cell Culture: Modulation by Aplysia Hemolymph and the Presence of the Initial Axonal Segment ,
J Neurosci
 3 (12) , 2403 , 1983
Cells
Neurons
LUQ cells
Enzymes
Protease : 1%
Medium
L15 medium
Neural
Shellfish
Aplysia californica
Camardo, J. , Proshansky, E. and Schacher, S.
Identified Aplysia Neurons Form Specific Chemical Synapses in Culture ,
J Neurosci
 3 (12) , 2614 , 1983
Cells
LUQ cells
RUQ cells
Enzymes
Protease : 1%
Medium
L15 medium
Reproductive
Insect
Drosophila
Ma, X. , Wang, S. , Do, T. , Song, X. , Inaba, M. , Nishimoto, Y. , Liu, L. , Gao, Y. , Mao, Y. , Li, H. , McDowell, W. , Park, J. , Malanowski, K. , Peak, A. , Perera, A. and Li, H. et al.
Piwi is Required in Multiple Cell Types to Control Germline Stem Cell Lineage Development in the Drosophila Ovary. ,
PLoS ONE
 9 , e90267 , 2014
Cells
Ovarian
Enzymes
Medium
Grace's
Reproductive
Insect
Drosophila, embryonic
Salmand, P. Iche-Torres, M. and Perrin, L.
Tissue-Specific Cell Sorting from Drosophila Embryos: Application to Gene Expression Analysis. , Fly (Austin) Vol. 5 , 261
Cells
Cardiac differentiating
Enzymes
Collagenase Type 1 : 20 u/ml
Trypsin : 0.25%
Medium
Schneider
Stem
Insect
Drosophila, larval
Berger, C. , Harzer, H. , Burkard, T. , Steinmann, J. , van der Horst, S. , Laurenson, A. , Novatchkova, M. , Reichert, H. and Knoblich, J.
FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal. ,
Cell Rep
 2 , 407 , 2012
Cells
Neuroblasts
Enzymes
Papain : 0.1%
Medium
Rinaldini solution

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.