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Tissue References Search Tool

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Adrenal
Ovine
Ovine, adult and fetal
Valego, N. and Rose, J.
A Specific CRH Antagonist Attenuates ACTH-Stimulated Cortisol Secretion in Ovine Adrenocortical Cells. ,
Reprod Sci
 17 , 477 , 2010
Cells
Adrenocortical
Enzymes
Medium
DMEM/Ham's F12
Adrenal
Ovine
Ovine, fetal
Valego, N. , Su, Y. , Carey, L. , Young, S. , Tatter, S. , Wang, J. and Rose, J.
Hypothalamic-Pituitary Disconnection in Fetal Sheep Blocks the Peripartum Increases in Adrenal Responsiveness and Adrenal ACTH Receptor Expression. ,
Am J Physiol Regul Integr Comp Physiol
 289 , R410 , 2005
Cells
Adrenocortical
Enzymes
Medium
DMEM/Ham's F12
Adrenal
Ovine
Ovine, adult
Keating, D. , Rychkov, G. , Adams, M. , Holgert, H. , McMillen, I.C. and Roberts, M.
Opioid Receptor Stimulation Suppresses the Adrenal Medulla Hypoxic Response in Sheep by Actions on Ca(2+) and K(+) Channels. ,
J Physiol
 555 , 489 , 2004
Cells
Chromaffin cells
Enzymes
Medium
Locke's solution
Adrenal
Ovine
Ovine, 3 year
Canny B J, O'Farrell K A, Clarke I J, Tilbrook A J.
The influence of sex and gonadectomy on the hypothalamo-pituitary-adrenal axis of the sheep ,
J Endocrinol
 162 , 215-25 , 1999
Cells
Anterior pituitary
Enzymes
Trypsin : 2.5%
Medium
DMEM
Brain
Insect
Drosophilia
Hadzic, T. , Park, D. , Abruzzi, K. , Yang, L. , Trigg, J. , Rohs, R. , Rosbash, M. and Taghert, P.
Genome-Wide Features of Neuroendocrine Regulation in Drosophila by the Basic Helix-Loop-Helix Transcription Factor DIMMED. ,
Nucleic Acids Res
 43 , 2199-215 , 2015
Cells
Neurons
Enzymes
Papain : 50 u/ml
Medium
Modified Dissecting Saline
Brain
Insect
Drosophila
Gu, H. and O'Dowd, D.
Whole Cell Recordings from Brain of Adult Drosophila. ,
J Vis Exp
 6 , 248 , 2007
Cells
Neurons
Enzymes
Papain : 20 u/ml
Medium
Saline
Brain
Insect
Drosophila
Sicaeros, B. Campusano, J. and O'Dowd, D.
Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae. ,
J Vis Exp
 4 , 200 , 2007
Cells
Neurons
Enzymes
Papain : 4 u/ml
Medium
DMEM
Brain
Insect
Drosophilia
Gu, H. and O'Dowd, D.
Cholinergic Synaptic Transmission in Adult Drosophila Kenyon Cells in Situ. ,
J Neurosci
 26 , 265-72 , 2006
Cells
Kenyon
Enzymes
Papain : 20 u/ml
Medium
Recording saline
Brain
Ovine
Ovine
Reddy, R. , Amodei, R. , Estill, C. , Stormshak, F. , Meaker, M. and Roselli, C.
Effect of Testosterone on Neuronal Morphology and Neuritic Growth of Fetal Lamb Hypothalamus-Preoptic Area and Cerebral Cortex in Primary Culture. ,
PLoS ONE
 10 , e0129521 , 2015
Cells
Neurons
Enzymes
Papain : 0.2%
Medium
Hibernate
Brain
Ovine
Ovine
Lepore G, Gadau S, Mura A, Zedda M, Farina V.
Aromatase Immunoreactivity in Fetal Ovine Neuronal Cell Cultures Exposed to Oxidative Injury. ,
Eur J Histochem
 53 , e28 , 2009
Cells
Neurons
Enzymes
PDS kit : per instructions
Medium
DMEM
Brain
Shellfish
Helisoma trivolvis
Hadley, R.D. , Bodnar, D.A. and Kater, S.B.
Formation of Electrical Synapses Between Isolated, Cultured Helisoma Neurons Requires Mutual Neurite Elongation ,
J Neurosci
 5 , 3145 , 1985
Cells
Neurons
Buccal ganglia
Enzymes
Trypsin : 0.2%
Medium
Saline, Sterile
Heart
Ovine
Ovine
Voigt, N. , Pearman, C. , Dobrev, D. and Dibb, K.
Methods for Isolating Atrial Cells from Large Mammals and Humans. ,
J Mol Cell Cardiol
 86 , 187-98 , 2015
Cells
Atrial myocytes
Enzymes
Protease XIV : 0.006%
Medium
Tyrode's solution
Heart
Ovine
Ovine
Dibb, K. , Clarke, J. , Horn, M. , Richards, M. , Graham, H. , Eisner, D. and Trafford, A.
Characterization of an Extensive Transverse Tubular Network in Sheep Atrial Myocytes and its Depletion in Heart Failure. ,
Circ Heart Fail
 2 , 482-9 , 2009
Cells
Cardiomyocytes
Enzymes
Protease Type XIV : 0.006%
Medium
See reference
Heart
Ovine
Ovine
Dhamoon, A, Pandit, S. , Sarmast, F. , Parisian, K. , Guha, P. , Li, Y. , Bagwe, S. , Taffet, S. and Anumonwo, J.
Unique Kir2.x Properties Determine Regional and Species Differences in the Cardiac Inward Rectifier K+ Current. ,
Circ Res
 94 , 1332-9 , 2004
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 100-200 u/ml
Medium
See reference
Heart
Shellfish
Pacific Oysters
Le Duff, R. , Lipart, C. and Renault, T.
Primary Culture of Pacific Oyster, Crassostrea gigas, heart cells ,
J Tiss Cul Meth
 16 , 67 , 1994
Cells
Haemocytes
Medium
L15 medium
Heart
Shellfish
Clams, 3.0 to 4.5 cm
Wen, C. , Kou, G. and Chen, S.
Cultivation of Cells From the Heart of the Hard Clam, Merretrix lusoria (Roding) ,
J Tiss Cul Meth
 15 , 123 , 1993
Cells
Myocytes
Fibroblasts
Enzymes
Medium
L15 medium
Miscellaneous
Insect
Insect, Lepidoptera
Goodwin, R. and McCawley, P
Initiating Attached Cell Lines From the Lepidoptera (Insecta) ,
Meth Cell Sci
 3 , 567 , 1977
Cells
Lepitopteran
Enzymes
Medium
Dulbecco PBS
Neural
Insect
Drosophila, larval
Berger, C. , Harzer, H. , Burkard, T. , Steinmann, J. , van der Horst, S. , Laurenson, A. , Novatchkova, M. , Reichert, H. and Knoblich, J.
FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal. ,
Cell Rep
 2 , 407 , 2012
Cells
Neuroblasts
Enzymes
Papain : 0.1%
Medium
Rinaldini solution
Neural
Insect
Drosophila
Sanchez-Soriano, N. , Bottenberg, W. , Fiala, A. , Haessler, U. , Kerassoviti, A. , Knust, E. , Lohr, R. and Prokop, A.
Are Dendrites in Drosophila Homologous to Vertebrate Dendrites? ,
Dev Biol
 288 , 126 , 2005
Cells
Dendrites
Enzymes
Collagenase : 0.05%
Medium
HBSS
Neural
Insect
Gryllus Bimaculatus
Kloppenburg, P. and Horner, M.
Voltage-Activated Currents in Identified Giant Interneurons Isolated from Adult Crickets Gryllus Bimaculatus ,
J Exp Biol
 201 (Pt 17) , 2529 , 1998
Cells
Giant interneurons
Enzymes
Collagenase : 0.05%
Medium
Leibovitz's L15
Neural
Shellfish
Aplysia californica
Lee, A. , Decourt, B. and Suter, D.
Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones. ,
J Vis Exp
 12 , 662 , 2008
Cells
Neurons
Enzymes
Medium
L-15-ASW
Neural
Shellfish
Snails (Helisoma trivolis), albino, adult
Zoran, M. , Doyle, R. and Haydon, P.
Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis ,
Neuron
 6 , 145 , 1991
Cells
Buccal ganglia; SLT muscle
Enzymes
Trypsin : 0.2%
Medium
DMEM
Neural
Shellfish
Pond snail (Helisoma) albino, adult
Haydon, P.
The Formation of Chemical Synapses Between Cell-Cultured Neuronal Somata ,
J Neurosci
 8 , 1032 , 1988
Cells
Somata, buccal ganglia
Enzymes
Trypsin : 0.2%
Medium
Antibiotic saline, Leibowitz 50%
Neural
Shellfish
Helisoma trivovlis
Cohan, C. , Haydon, P. and Kater, S.
Single Channel Activity Differs in Growing and Nongrowing Growth Cones of Isolated Identified Neurons of Helisoma ,
J Neurosci Res
 13 , 285 , 1985
Cells
Buccal ganglia
Enzymes
Trypsin : 0.2%
Medium
L-15 medium
Neural
Shellfish
Mollusc, juvenile 1-2 gm or adult 50-100 gm
Schacher, S. and Proshanksy, E.
Neurite Regeneration by Aplysia Neurons in Dissociated Cell Culture: Modulation by Aplysia Hemolymph and the Presence of the Initial Axonal Segment ,
J Neurosci
 3 (12) , 2403 , 1983
Cells
Neurons
LUQ cells
Enzymes
Protease : 1%
Medium
L15 medium
Neural
Shellfish
Aplysia californica
Camardo, J. , Proshansky, E. and Schacher, S.
Identified Aplysia Neurons Form Specific Chemical Synapses in Culture ,
J Neurosci
 3 (12) , 2614 , 1983
Cells
LUQ cells
RUQ cells
Enzymes
Protease : 1%
Medium
L15 medium
Pituitary
Ovine
Ovine, adult
Xu Ruwei, Wang Qinling, Yan Ming, Hernandez Maria, Gong Changhong, Boon WahChin, Murata Yoko, Ueta Yoichi, Chen Chen.
Orexin-A augments voltage-gated Ca2+ currents and synergistically increases growth hormone (GH) secretion with GH-releasing hormone in primary cultured ovine somatotropes ,
Endocrinology
 143 , 4609-19 , 2002
Cells
Somatotropes
Enzymes
Medium
Medium 199
Reproductive
Insect
Drosophila
Ma, X. , Wang, S. , Do, T. , Song, X. , Inaba, M. , Nishimoto, Y. , Liu, L. , Gao, Y. , Mao, Y. , Li, H. , McDowell, W. , Park, J. , Malanowski, K. , Peak, A. , Perera, A. and Li, H. et al.
Piwi is Required in Multiple Cell Types to Control Germline Stem Cell Lineage Development in the Drosophila Ovary. ,
PLoS ONE
 9 , e90267 , 2014
Cells
Ovarian
Enzymes
Medium
Grace's
Reproductive
Insect
Drosophila, embryonic
Salmand, P. Iche-Torres, M. and Perrin, L.
Tissue-Specific Cell Sorting from Drosophila Embryos: Application to Gene Expression Analysis. , Fly (Austin) Vol. 5 , 261
Cells
Cardiac differentiating
Enzymes
Collagenase Type 1 : 20 u/ml
Trypsin : 0.25%
Medium
Schneider
Reproductive
Ovine
Ovine, 3-5 year
Letouzey, V. , Tan, K. , Deane, J. , Ulrich, D. , Gurung, S. , Ong, Y. and Gargett, C.
Isolation and Characterisation of Mesenchymal Stem/Stromal Cells in the Ovine Endometrium. ,
PLoS ONE
 10 , e0127531 , 2015
Cells
Endometrial stromal
Enzymes
Medium
DMEM/F-12
Stem
Insect
Drosophila, larval
Berger, C. , Harzer, H. , Burkard, T. , Steinmann, J. , van der Horst, S. , Laurenson, A. , Novatchkova, M. , Reichert, H. and Knoblich, J.
FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal. ,
Cell Rep
 2 , 407 , 2012
Cells
Neuroblasts
Enzymes
Papain : 0.1%
Medium
Rinaldini solution
Stem
Ovine
Ovine, 3-5 year
Letouzey, V. , Tan, K. , Deane, J. , Ulrich, D. , Gurung, S. , Ong, Y. and Gargett, C.
Isolation and Characterisation of Mesenchymal Stem/Stromal Cells in the Ovine Endometrium. ,
PLoS ONE
 10 , e0127531 , 2015
Cells
Endometrial stromal
Enzymes
Medium
DMEM/F-12

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.