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Tissue References Search Tool

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Adipose/Fat
Bear
Bear, Ursus arctos
Gehring, J. , Rigano, K. , Evans, H. , Nelson, O. , Robbins, C. and Jansen, H.
A Protocol for the Isolation and Cultivation of Brown Bear (Ursus arctos) Adipocytes. ,
Cytotechnology
 68 , 2177-91 , 2016
Cells
Adipocytes
Enzymes
Medium
HBSS
Brain
Insect
Drosophilia
Hadzic, T. , Park, D. , Abruzzi, K. , Yang, L. , Trigg, J. , Rohs, R. , Rosbash, M. and Taghert, P.
Genome-Wide Features of Neuroendocrine Regulation in Drosophila by the Basic Helix-Loop-Helix Transcription Factor DIMMED. ,
Nucleic Acids Res
 43 , 2199-215 , 2015
Cells
Neurons
Enzymes
Papain : 50 u/ml
Medium
Modified Dissecting Saline
Brain
Insect
Drosophila
Gu, H. and O'Dowd, D.
Whole Cell Recordings from Brain of Adult Drosophila. ,
J Vis Exp
 6 , 248 , 2007
Cells
Neurons
Enzymes
Papain : 20 u/ml
Medium
Saline
Brain
Insect
Drosophila
Sicaeros, B. Campusano, J. and O'Dowd, D.
Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae. ,
J Vis Exp
 4 , 200 , 2007
Cells
Neurons
Enzymes
Papain : 4 u/ml
Medium
DMEM
Brain
Insect
Drosophilia
Gu, H. and O'Dowd, D.
Cholinergic Synaptic Transmission in Adult Drosophila Kenyon Cells in Situ. ,
J Neurosci
 26 , 265-72 , 2006
Cells
Kenyon
Enzymes
Papain : 20 u/ml
Medium
Recording saline
Heart
Invertebrate
Octopus, E. cirhosa, 260-352 g
Altimiras J. , Hove-Madsen L. and Gesser H.
Ca(2+) Uptake in the Sarcoplasmic Reticulum from the Systemic Heart of Octopod Cephalopods ,
J Exp Biol
 202 , 2531 , 1999
Cells
Systemic heart cardiomyocytes
Enzymes
Collagenase : 0.025%
Trypsin : 0.02%
Medium
see reference
Miscellaneous
Insect
Insect, Lepidoptera
Goodwin, R. and McCawley, P
Initiating Attached Cell Lines From the Lepidoptera (Insecta) ,
Meth Cell Sci
 3 , 567 , 1977
Cells
Lepitopteran
Enzymes
Medium
Dulbecco PBS
Miscellaneous
Invertebrate

Octopus vulgaris

Maselli, V. , Xu, F. , Syed, N. , Polese, G. and Cosmo, A.
A Novel Approach to Primary Cell Culture for Octopus vulgaris Neurons. ,
Front Physiol
 9 , 220 , 2018
Cells
Neurons
Enzymes
Papain : 0.1%
Trypsin : 0.1%
Medium
L15-ASW
Miscellaneous
Invertebrate
Squid
Raman, M. and Mathew, S.
Study of Chemical Properties and Evaluation of Collagen in Mantle, Epidermal Connective Tissue and Tentacle of Indian Squid, Loligo duvauceli Orbigny. ,
J Food Sci Technol
 51 , 1509-16 , 2014
Cells
Mantle
Enzymes
Collagenase : see reference
Papain : see reference
Medium
Phosphate buffer
Neural
Insect
Drosophila, larval
Berger, C. , Harzer, H. , Burkard, T. , Steinmann, J. , van der Horst, S. , Laurenson, A. , Novatchkova, M. , Reichert, H. and Knoblich, J.
FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal. ,
Cell Rep
 2 , 407 , 2012
Cells
Neuroblasts
Enzymes
Papain : 0.1%
Medium
Rinaldini solution
Neural
Insect
Drosophila
Sanchez-Soriano, N. , Bottenberg, W. , Fiala, A. , Haessler, U. , Kerassoviti, A. , Knust, E. , Lohr, R. and Prokop, A.
Are Dendrites in Drosophila Homologous to Vertebrate Dendrites? ,
Dev Biol
 288 , 126 , 2005
Cells
Dendrites
Enzymes
Collagenase : 0.05%
Medium
HBSS
Neural
Insect
Gryllus Bimaculatus
Kloppenburg, P. and Horner, M.
Voltage-Activated Currents in Identified Giant Interneurons Isolated from Adult Crickets Gryllus Bimaculatus ,
J Exp Biol
 201 (Pt 17) , 2529 , 1998
Cells
Giant interneurons
Enzymes
Collagenase : 0.05%
Medium
Leibovitz's L15
Reproductive
Insect
Drosophila
Ma, X. , Wang, S. , Do, T. , Song, X. , Inaba, M. , Nishimoto, Y. , Liu, L. , Gao, Y. , Mao, Y. , Li, H. , McDowell, W. , Park, J. , Malanowski, K. , Peak, A. , Perera, A. and Li, H. et al.
Piwi is Required in Multiple Cell Types to Control Germline Stem Cell Lineage Development in the Drosophila Ovary. ,
PLoS ONE
 9 , e90267 , 2014
Cells
Ovarian
Enzymes
Medium
Grace's
Reproductive
Insect
Drosophila, embryonic
Salmand, P. Iche-Torres, M. and Perrin, L.
Tissue-Specific Cell Sorting from Drosophila Embryos: Application to Gene Expression Analysis. , Fly (Austin) Vol. 5 , 261
Cells
Cardiac differentiating
Enzymes
Collagenase Type 1 : 20 u/ml
Trypsin : 0.25%
Medium
Schneider
Stem
Insect
Drosophila, larval
Berger, C. , Harzer, H. , Burkard, T. , Steinmann, J. , van der Horst, S. , Laurenson, A. , Novatchkova, M. , Reichert, H. and Knoblich, J.
FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal. ,
Cell Rep
 2 , 407 , 2012
Cells
Neuroblasts
Enzymes
Papain : 0.1%
Medium
Rinaldini solution

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.