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Tissue References Search Tool

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Adipose/Fat
Porcine
Porcine, crossbred, 1-3 day
Suryawan, A. , Swanson, L. and Hu, C.
Insulin and Hydrocortisone, But Not Triiodothyronine, Are Required for the Differentiation of Pig Preadipocytes in Primary Culture ,
J Anim Sci
 75 , 105 , 1997
Cells
Adipose, Stromal-vascular
Enzymes
Medium
DMEM/F12
Adipose/Fat
Rat
Rat, 9 week
Ohta, Y. , Hamaguchi, A. , Ootaki, M. , Watanabe, M. , Takeba, Y. , Iiri, T. , Matsumoto, N. and Takenaga, M.
Intravenous Infusion of Adipose-Derived Stem/Stromal Cells Improves Functional Recovery of Rats with Spinal Cord Injury. ,
Cytotherapy
 19 , 839-848 , 2017
Cells
Adipose stromal
Enzymes
Collagenase : 0.1%
Medium
DMEM
Adipose/Fat
Rat
Rat, SD, 2-3 month
Dayer, D. , Tabar, M. , Moghimipour, E. , Tabandeh, M. , Ghadiri, A. , Bakhshi, E. , Orazizadeh, M. and Ghafari, M.
Sonic Hedgehog Pathway Suppression and Reactivation Accelerates Differentiation of Rat Adipose-Derived Mesenchymal Stromal Cells Toward Insulin-Producing Cells ,
Cytotherapy
 19 , 937-946 , 2017
Cells
Mesenchymal stromal
Enzymes
Medium
DMEM
Adipose/Fat
Rat
Rat
Emre, E, Yuksel, N, Duruksu, G, Pirhan, D, Subasi, C, Erman, G. and Karaoz, E
Neuroprotective Effects of Intravitreally Transplanted Adipose Tissue and Bone Marrow-Derived Mesenchymal Stem Cells in an Experimental Ocular Hypertension Model. ,
Cytotherapy
 17 , 543-59 , 2015
Cells
Mesenchymal stem
Enzymes
Medium
DMEM
Adipose/Fat
Rat
Rat
Ogasawara, J. , Izawa, T. , Sakurai, T. , Shirato, K. , Ishibashi, Y. , Ohira, Y. , Ishida, H. , Ohno, H. and Kizaki, T.
Habitual Exercise Training Acts as a Physiological Stimulator for Constant Activation of Lipolytic Enzymes in Rat Primary White Adipocytes. ,
Biochem Biophys Res Commun
 464 , 348-53 , 2015
Cells
Adipocytes
Enzymes
Medium
Krebs-Ringer
Adipose/Fat
Rat
Rat, SD, 5 month
Veronesi, F, Torricelli, P, Della, B, Pagani, S. and Fini, M
In Vitro Mutual Interaction Between Tenocytes and Adipose-Derived Mesenchymal Stromal Cells. ,
Cytotherapy
 17 , 215-23 , 2015
Cells
Mesenchymal stromal
Enzymes
Medium
DMEM
Adipose/Fat
Rat
Rat, adult
Leblanc, A. , Nguyen, Q. , Touroo, J. , Aird, A. , Chang, R. , Ng, C. , Hoying, J. and Williams, S.
Adipose-derived Cell Construct Stabilizes Heart Function and Increases Microvascular Perfusion in an Established Infarct. ,
Stem Cells Transl Med
 2 , 896-905 , 2013
Cells
Stromal vascular
Enzymes
Medium
PBS
Adipose/Fat
Rat
Rat, male, 300g
Thompson, A. , Nunez, M. , Davidson, R. , Horm, T. , Schnittker, K. , Hart, M. , Suarez, A. and Tsao, T.
Mitigation of Isolation-Associated Adipocyte Interleukin-6 Secretion Following Rapid Dissociation of Adipose Tissue. ,
J Lipid Res
 53 , 2797 , 2012
Cells
Adipocytes
Enzymes
Medium
RPMI 1640
Adipose/Fat
Rat
Rat, Lewis, male
Basu, J. , Genheimer, C. , Sangha, N. , Quinlan, S. , Guthrie, K. , Kelley, R. , Ilagan, R. , Jain, D. , Bertram, T. and Ludlow, J.
Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. ,
Lipids Health Dis
 10 , 171 , 2011
Cells
Renal adipose derived cells
Enzymes
Medium
DMEM
Adipose/Fat
Rat
Rat, SD, neonatal
Liu, Z. , Wang, H. , Zhang, Y. , Zhou, J. , Lin, Q. , Wang, Y. , Duan, C. , Wu, K. and Wang, C.
Efficient Isolation of Cardiac Stem Cells from Brown Adipose. ,
J Biomed Biotechnol
 2010 , 104296 , 2010
Cells
Brown adipocytes
Enzymes
Medium
PBS
Adipose/Fat
Rat
Rat
Wei, X. , Du, Z. , Zhao, L. , Feng, D. , Wei, G. , He, Y. , Tan, J. , Lee, W. , Hampel, H. , Dodel, R. , Johnstone, B. , March, K. , Farlow, M. and Du, Y.
IFATS Collection: The Conditioned Media of Adipose Stromal Cells Protect Against Hypoxia-Ischemia-Induced Brain Damage In Neonatal Rats. ,
Stem Cells
 27 , 478 , 2009
Cells
Adipose derived stem
Enzymes
Medium
DMEM
Adipose/Fat
Rat
Rat
Tomiyama, K. , Murase, N. , Stolz, D. , Toyokawa, H. , O'Donnell, D. , Smith, D. , Dudas, J. , Rubin, J. and Marra, K.
Characterization of Transplanted Green Fluorescent Protein+ Bone Marrow Cells Into Adipose Tissue. ,
Stem Cells
 26 , 330 , 2008
Cells
Adipose tissue-derived stem
Enzymes
Medium
DMEM
Adipose/Fat
Rat
Rat, Wistar, 4 week
Aoki, S. , Toda, S. , Sakemi, T. and Sugihara, H.
Coculture of Endothelial Cells and Mature Adipocytes Actively Promotes Immature Preadipocyte Development In Vitro ,
Cell Struct Funct
 28 , 55 , 2003
Cells
Adipocytes
Enzymes
Collagenase : 0.2%
Medium
Ham's F12
Adipose/Fat
Rat
Rat, SD
Mora, S. , Yang, C. , Ryder, J. , Boeglin, D. and Pessin, J
The MEF2A and MEF2D Isoforms are Differentially Regulated in Muscle and Adipose Tissue during States of Insulin Deficiency ,
Endocrinology
 142 , 1999 , 2001
Cells
Adipocytes
Enzymes
Medium
KRHB
Adipose/Fat
Rat
Rat, SD, male, 4-7 weeks
Omatsu-Kanbe, M. and Matsuura, H.
Inhibition of Store-operated Ca2+ Entry by Extracellular ATP in Rat Brown Adipocytes ,
J Physiol
 521 (3) , 601 , 1999
Cells
Brown adipocytes
Enzymes
Medium
DMEM
Adipose/Fat
Rat
Rat
Serrero, G.
Primary Culture in Defined Medium of Adipocyte Precursors , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 11B:6.1 , 1995
Cells
Adipocytes
Enzymes
Medium
DMEM /F-12
Adipose/Fat
Rat
Rat, SD, male, 130-160 g
Charron, M.J. and Kahn, B.B.
Divergent Molecular Mechanisms for Insulin-Resistant Glucose Transport in Muscle and Adipose Cells In Vivo ,
J Biol Chem
 265 , 7994 , 1990
Cells
Adipose
Epididymal fat pads
Enzymes
Collagenase : 0.3%
Medium
Kreb's-Ringer bicarbonate buffer modified
Adipose/Fat
Rat
Rat, 3 day
Gaben-Cogneville, A. , Poussin, B. , Chamblier, M. , Forgue-Fafitte, M. and Rosselin, G.
Development of Insulin and Epidermal Growth Factor Receptors During the Differentiation of Rat Preadipocytes in Primary Culture ,
Biochim Biophys Acta
 968 , 231 , 1988
Cells
Preadipocytes
Enzymes
Medium
Parker Medium 199
Adipose/Fat
Rat
Rat, SD, various weights and ages
Woodward, Julie A. and Saggerson, E.
Effect of Adenosine Deaminase, N6-Phenylisopropyladenosine and Hypothroidism on the Responsiveness of Rat Brown Adipocytes to Noradrenaline ,
Biochem J
 238 , 395 , 1986
Cells
Brown adipocytes
Interscapular & cervical depots
Enzymes
Medium
Krebs Ringer bicarbonate buffer
Adipose/Fat
Rat
Rat, CD, male, 150-200 g
Pessin, J.E. , Gitomer, W. , Oka, Y. , Oppenheimer, C.L. and Czech, M.P.
ß-Adrenergic Regulation of Insulin and Epidermal Growth Factor Receptors in Rat Adipocytes ,
J Biol Chem
 258 , 7386 , 1983
Cells
Adipocytes
Epididymal fat pads
Enzymes
Collagenase : 0.1%
Medium
Krebs Ringer bicarbonate buffer
Adipose/Fat
Rat
Rat, Wistar, albino, male, 100-140 g
Green, A. and Newsholme, E.
Sensitivity of Glucose Uptake and Lipolysis of White Adipocytes of the Rat to Insulin and Effects of Some Metabolites ,
Biochem J
 180 , 365 , 1979
Cells
Adipocytes
Epididymal-fat pads
Enzymes
Collagenase : 0.3%
Medium
Kreb's Ringer
Adipose/Fat
Rat
Rat, Fischer, 344, male, 9 - 13 week
Stiles, J.W. , Francendese, A.A. and Masoro, E.J.
Influence of Age on Size and Number of Fat Cells in the Epididymal Depot ,
Am J Physiol
 229 , 1561 , 1975
Cells
White fat
Enzymes
Collagenase : 0.3%
Medium
Kreb's Ringer bicarbonate buffer
Adipose/Fat
Rat
Rat, Wistar, male, 5 week-16 month
DiGirolamo, M. , Mendlinger, S. and Fertig, J.W.
A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species ,
Am J Physiol
 221 , 850 , 1971
Cells
White fat
Enzymes
Collagenase : 0.05%
Medium
Kreb's Ringer bicarbonate buffer
Adipose/Fat
Rat
Rat (CFE), albino, female
Fain, J. , Reed, N. and Saperstein, R.
Isolation and Metabolism of Brown Fat Cells ,
J Biol Chem
 242 , 1887 , 1967
Cells
Brown fat
Enzymes
Medium
Bicarbonate buffer
Adipose/Fat
Rat
Rat, SD, male, 160-210 g
Rodbell, M.
Metabolism of Isolated Fat Cells. I. Effects of Hormones on Glucose Metabolism and Lipolysis ,
J Biol Chem
 239 (2) , 375 , 1964
Cells
Fat
Enzymes
Collagenase : 0.3%
Medium
Albumin-bicarbonate buffer
Adipose/Fat
Squirrel
Squirrel
McFarlane, S. , Mathers, K. and Staples, J.
Reversible Temperature-Dependent Differences in Brown Adipose Tissue Respiration During Torpor in a Mammalian Hibernator. ,
Am J Physiol Regul Integr Comp Physiol
 312 , R434-R442 , 2017
Cells
Brown adipocytes
Enzymes
Medium
Krebs-Ringer phosphate
Neural
Avian
Quail and Chicken, embryonic
Rollo, B. , Zhang, D. , Simkin, J. , Menheniott, T. and Newgreen, D.
Why are Enteric Ganglia so Small? Role of Differential Adhesion of Enteric Neurons and Enteric Neural Crest Cells. ,
F1000Res
 4 , 113 , 2015
Cells
Midgut DRG
Enzymes
Medium
Ham's F12
Neural
Avian
Chick, White Leghorn, embryos
Temburni, M. , Rosenberg, M. , Pathak, N. , McConnell, R. and Jacob, M.
Neuronal Nicotinic Synapse Assembly Requires the Adenomatous Polyposis Coli Tumor Suppressor Protein. ,
J Neurosci
 24 , 6776 , 2004
Cells
Cilary ganglion neurons
Enzymes
Medium
PBS
Neural
Bovine
Bovine
(also rat)
Trifaro, J. , Tang, R. and Novas, M.
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
 26 , 335 , 1990
Cells
Heart
Adrenal chromaffin
Paraneurons
Enzymes
Trypsin : 0.06%
Medium
25mM HEPES buffered Locke's solution, CMF
Neural
Bovine
Bovine
Bowman, P. , Betz, A. and Goldstein, G.
Primary Culture of Microvascular Endothelial Cells From Bovine Retina ,
In Vitro
 18 (7) , 626 , 1982
Cells
Microvascular endothelial
Enzymes
Medium
MEM
Neural
Bovine
Calf
(also lamb)
Poduslo, S. , Miller, K. and McKhann, G.
Metabolic Properties of Maintained Oligodendroglia Purified from Brain ,
J Biol Chem
 253 , 1592 , 1978
Cells
Oligodendroglia
Neural
Enzymes
Trypsin : 0.1%
Medium
(see reference)
Neural
Canine
Canine
Tongtako, W. , Lehmbecker, A. , Wang, Y. , Hahn, K. , Baumgartner, W. and Gerhauser, I.
Canine Dorsal Root Ganglia Satellite Glial Cells Represent an Exceptional Cell Population with Astrocytic and Oligodendrocytic Properties. ,
Sci Rep
 7 , 13915 , 2017
Cells
Satellite glial
Enzymes
Medium
HBSS
Neural
Chicken
Chick, White Leghorn, embryos
Watt, P. , Mullen, Y. , Benhamou, P. , Hober, C. , Nomura, Y. , Watanabe, Y. , Passaro, E. , Zinner, M. and Brunicardi, F.
Simplified Semiautomated Method For Isolating Islets From the Human Pancreas ,
Transplant Proc
 26 (2) , 582 , 1994
Cells
Islets
Enzymes
Trypsin : Trypsin: 0.125-0.25%
Collagenase : 0.2%
Deoxyribonuclease I : 200 u/ml
Medium
Eurocollins solution
Neural
Chicken
Chicken, White Leghorn, embryos, 17-21 day
Raman, I. and Trussell, L.
The Kinetics of the Response to Glutamate and Kainte in Neurons of the Avian and Cochlear Nucleus ,
Neuron
 9 , 173 , 1992
Cells
Neurons
Enzymes
Papain : 40 u/ml
Medium
HEPES
Neural
Chicken
Chick, embryo, 10-14 day
Moyer, M. , Bullrich, F. and Sheffield, J.
Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina ,
In Vitro Cell Dev Biol
 26 , 1073 , 1990
Cells
Flat, retina
Enzymes
Trypsin : 0.1%
Medium
Tyrode's solution, CMF
Neural
Chicken
Chick, White Leghorn, embryo
(also rat, SD, embryo)
Schnaar, R. and Schaffner, A.
Separation of cell types from embryonic chicken and rat spinal cord: characterization of motoneuron-enriched fractions ,
J Neurosci
 1 , 204 , 1981
Cells
Spinal cord
Enzymes
Trypsin : 0.05%
Medium
Phosphate buffer
(see reference)

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.