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Tissue References Search Tool

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Adrenal
Human
Human, adult and child
Folkman, J. , Haudenschild, C. C. and Zetter, B. R.
Long-term Culture of Capillary Endothlial Cells ,
Proc Natl Acad Sci U S A
 76 , 5217 , 1979
Cells
Foreskin
Enzymes
Collagenase : 0.5%
Medium
Dulbecco's MEM w/10% calf serum
Adrenal
Mouse
Mouse
Dominguez, N. , Rodri­guez, M. , Machado, J. and Borges, R.
Preparation and Culture of Adrenal Chromaffin Cells. ,
Methods Mol Biol
 846 , 223 , 2012
Cells
Chromaffin
Enzymes
Collagenase Type 1 : 0.1-0.2%
Deoxyribonuclease I : 0.003-0.015%
Hyaluronidase : 0.015%
Medium
DMEM/F12
Adrenal
Mouse
Mouse, 8-10 week
Kolski-Andreaco, A. , Cai, H. , Currle, D. , Chandy, K. and Chow, R.
Mouse Adrenal Chromaffin Cell Isolation. ,
J Vis Exp
 2 , 129 , 2007
Cells
Chromaffin
Enzymes
Papain : 40 u/ml
Medium
DMEM
Adrenal
Mouse
Mouse, embryonic
Tian Jin-Hua, Wu Zheng-Xing, Unzicker Michael, Lu Li, Cai Qian, Li Cuiling, Schirra Claudia, Matti Ulf, Stevens David, Deng Chuxia, Rettig Jens, Sheng Zu-Hang.
The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells ,
J Neurosci
 25 , 10546-55 , 2005
Cells
Chromafin cells
Enzymes
Papain : 20-25 u/ml
Medium
DMEM
Adrenal
Ovine
Ovine, adult and fetal
Valego, N. and Rose, J.
A Specific CRH Antagonist Attenuates ACTH-Stimulated Cortisol Secretion in Ovine Adrenocortical Cells. ,
Reprod Sci
 17 , 477 , 2010
Cells
Adrenocortical
Enzymes
Medium
DMEM/Ham's F12
Adrenal
Ovine
Ovine, fetal
Valego, N. , Su, Y. , Carey, L. , Young, S. , Tatter, S. , Wang, J. and Rose, J.
Hypothalamic-Pituitary Disconnection in Fetal Sheep Blocks the Peripartum Increases in Adrenal Responsiveness and Adrenal ACTH Receptor Expression. ,
Am J Physiol Regul Integr Comp Physiol
 289 , R410 , 2005
Cells
Adrenocortical
Enzymes
Medium
DMEM/Ham's F12
Adrenal
Ovine
Ovine, adult
Keating, D. , Rychkov, G. , Adams, M. , Holgert, H. , McMillen, I.C. and Roberts, M.
Opioid Receptor Stimulation Suppresses the Adrenal Medulla Hypoxic Response in Sheep by Actions on Ca(2+) and K(+) Channels. ,
J Physiol
 555 , 489 , 2004
Cells
Chromaffin cells
Enzymes
Medium
Locke's solution
Adrenal
Ovine
Ovine, 3 year
Canny B J, O'Farrell K A, Clarke I J, Tilbrook A J.
The influence of sex and gonadectomy on the hypothalamo-pituitary-adrenal axis of the sheep ,
J Endocrinol
 162 , 215-25 , 1999
Cells
Anterior pituitary
Enzymes
Trypsin : 2.5%
Medium
DMEM
Adrenal
Rat
Rat
Dominguez, N. , Rodri­guez, M. , Machado, J. and Borges, R.
Preparation and Culture of Adrenal Chromaffin Cells. ,
Methods Mol Biol
 846 , 223 , 2012
Cells
Chromaffin
Enzymes
Collagenase Type 1 : 0.1-0.2%
Deoxyribonuclease I : 0.003-0.015%
Hyaluronidase : 0.015%
Medium
DMEM/F12
Adrenal
Rat
Rat
Gilabert, J, Montalvo, G. and Artalejo A.
Rat Chromaffin Cells Primary Cultures: Standardization and Quality Assessment for Single-Cell Assays ,
Nat Protoc
 , 294 , 2006
Cells
Chromaffin
Enzymes
Medium
HBSS
Adrenal
Rat
Rat, SD
Gilabert, J.
Necessary Conditions to Maintain Rat Adrenal Chromaffin Cells in Primary Culture , Cell Biology of the Chromaffin Cell , Borges, R. and Gandia, L. , Instituto Teofilo Hernando , 2004
Cells
Chromaffin cells
Enzymes
Medium
HBSS
Adrenal
Rat
Rat, SD, male
Bruder Eric D, Ball Dennis L, Goodfriend Theodore L, Raff Hershel.
An oxidized metabolite of linoleic acid stimulates corticosterone production by rat adrenal cells ,
Am J Physiol Regul Integr Comp Physiol
 284 , R1631-5 , 2003
Cells
Zona fasciculata/reticularis
Enzymes
Collagenase : 0.4%
Medium
Krebs-HEPES
Adrenal
Rat
Rat, Wistar, newborn
Zhang L, Castell A, Avila E, Drucker-Colín R, Escobar A.
Immunocytochemical, ultrastructural and neurochemical evidences on synaptogenesis and dopamine release of rat chromaffin cells co-cultured with striatal neurons ,
J Neuropathol Exp Neurol
 59 , 170-4 , 2000
Cells
Chromaffin cells
Enzymes
Medium
DMEM
Adrenal
Rat
Rat, SD, male
Sayed, S. , Whitehouse, B. and Jones, P.
Phosphoserine/Threonine Phosphatases in the Rat Adrenal Cortex: A Role in the Control of Steroidogenesis ,
J Endocrinol
 154 , 449 , 1997
Cells
ZG
ZFR
Enzymes
Medium
Kreb's
Adrenal
Rat
Rat, Fischer, male, 10-16 weeks
Roskelley, C.D. and Auersperg, N.
Density Separation of Rat Adrenocortical Cells: Morphology, Steroidogenesis, and P-450scc Expression in Primary Culture ,
In Vitro Cell Dev Biol
 26 , 493 , 1990
Cells
Adrenocortical
Enzymes
Medium
BSS
Adrenal
Rat
Rat, SD, 2-4 day old
(also bovine)
Trifaro, J. , Tang, R. and Novas, M.
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
 26 , 335 , 1990
Cells
Heart
Adrenal chromaffin
Paraneurons
Enzymes
Trypsin : 0.06%
Medium
25mM HEPES buffered Locke's solution, CMF
Adrenal
Rat
Rat, male, 120-160 g,
Rat, SD, male, 400-450 g
Ng, T. and Liu, W.
Toxic Effect of Heavy Metals on Cells Isolated from the Rat Adrenal and Testis ,
In Vitro Cell Dev Biol
 26 , 24 , 1990
Cells
Leydig
Adrenal
Enzymes
Collagenase Type 2 : 0.03% (adrenal)
Medium
Krebs Ringer bicarbonate buffer
Adrenal
Rat
Rat, Long-Evans, female, 150-200 g
Payet, N. , Deziel, Y. and Lehoux, J.-G.
Vasopressin: A Potent Growth Factor in Adrenal Glomerulosa Cells in Culture ,
J Steroid Biochem
 20 , 449 , 1984
Cells
Glomerulosa
Enzymes
Collagenase : 0.2%
Medium
MEM-d-Val
Adrenal
Rat
Rat, Fischer, male, 1-10 months
Leonard, R.K. , Auersperg, N. and Parkes, C.O.
Ascorbic Acid Accumulation by Cultured Rat Adrenocortical Cells ,
In Vitro
 19 , 46 , 1983
Cells
Adrenocortical
Enzymes
Medium
BSS
Adrenal
Rat
Rat, SD, male, 400-450 g
Li, C.H. , Ng, T.B. and Cheng, C.H.K.
Melanotropins: Aldosterone- and Corticosterone-Stimulating Activity in Isolated Rat Adrenal Cells ,
Int J Pept Protein Res
 19 , 361 , 1982
Cells
Decapular
Capsular
Glomerulosa
Enzymes
Medium
Medium 199
Adrenal
Rat
Rat, Hanover-Wistar, 2nd postnatal week (also guinea-pig, cattle)
Unsicker, K. , Rieffert, B. and Ziegler, W.
Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro ,
Adv Biochem Psychopharmacol
 255 , 51 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.5%
Collagenase : 0.5%
Medium
HBSS
Adrenal
Rat
Rat , SD, female, 200 g
Braley, L. , Williams, G. and Bradwin, G.
The Effect of Unit Gravity Sedimentation on Adrenal Steroidogenesis by Isolated Rat Glomerulosa and Fasciculata Cells ,
Endocrinology
 106 (1) , 50 , 1980
Cells
Glomerulosa
Enzymes
Medium
Kreb's
Adrenal
Rat
Rat
Englert, D.F.
An Optical Study of Isolated Rat Adrenal Chromaffin Cells ,
Exp Cell Res
 125 , 369 , 1980
Cells
Chromaffin
Enzymes
Trypsin : 0.10%
Medium
Ham's F-12 w/HEPES
Adrenal
Rat
Rat
Folkman, J. , Haudenschild, C. C. and Zetter, B. R.
Long-term Culture of Capillary Endothlial Cells ,
Proc Natl Acad Sci U S A
 76 , 5217 , 1979
Cells
Foreskin
Enzymes
Collagenase : 0.5%
Medium
Dulbecco's MEM w/10% calf serum
Adrenal
Rat
Rat, Wistar-Hanover, 7-12 day
Unsicker, K. , Krisch, B. , Otten, U. and Thoenen, H.
Nerve Growth Factor-Induced Fiber Outgrowth From Isolated Rat Adrenal Chromaffin Cells: Impairment by Glucocorticoids ,
Proc Natl Acad Sci U S A
 75 (7) , 3498 , 1978
Cells
Medullary
Enzymes
Trypsin : 0.125%
Medium
HBSS
Adrenal
Rat
Rat, SD, male
Barofsky, A. , Feinstein, M. and Halkerston, I.
Enzymatic and Mechanical Requirements for the Dissociation of Cortical Cells From Rat Adrenal Glands ,
Exp Cell Res
 79 , 263 , 1973
Cells
Cortical
Enzymes
Trypsin : 0.25%
Medium
Kreb's Ringer bicarbonate buffer
Adrenal
Rat
Rat, Holtzman, male, 180-250 g
Kloppenborg, P. , Island, D. , Liddle, G. , Michelakis, A. and Nicholson, W.
A Method of Preparing Adrenal Cell Suspensions and Its Applicability to the In Vitro Study of Adrenal Metabolism ,
Endocrinology
 82 , 1053 , 1968
Cells
Adrenal
Enzymes
Medium
Kreb's Ringer bicarbonate buffer
Lung
Bovine
Bovine
Del Vecchio, P. , Siflinger-Birnboim, A. , Belloni, P. , Holleran, L. , Lum, H. and Malik, A.
Culture and Characterization of Pulmonary Microvascular Endothelial Cells ,
In Vitro Cell Dev Biol
 28A , 711 , 1992
Cells
Endothelial
Pulmonary
Enzymes
Collagenase : 1000 u/ml
Collagenase Type 2 : 1000 u/ml
Medium
PBS, CMF
Lung
Guinea-Pig
Guinea-pig, male, 250-300 g
Sikpi, M. , Nair, C. , Johns, A. and Das, S.
Metabolic and Ultrastructural Characterizationof Guinea-Pig Alveolar Type II Cells Isolated by Centrifugal Elutriation ,
Biochim Biophys Acta
 877 , 20 , 1986
Cells
Alveolar type II
Enzymes
Elastase : 40 u/ml
Medium
PBS
(see reference)
Lung
Guinea-Pig
Guinea-pig, male, 100 g
Phillips, H.
Dissociation of Single Cells from Lung or Kidney Tissue with Elastase ,
In Vitro
 8 , 101 , 1972
Cells
Single cells
Enzymes
Trypsin : 0.25%
Medium
CF salt solution
Lung
Human

Human

Konishi, S. , Tata, A. and Tata, P.
Defined Conditions for Long-term Expansion of Murine and Human Alveolar Epithelial Stem Cells in Three-dimensional Cultures. , STAR Protoc Vol. 3 , 101447 , 2022
Cells
Alveolar epithelial stem
Enzymes
Collagenase Type 1 : 450 u/ml
Neutral Protease : 5 u/ml
Deoxyribonuclease I : 0.33 u/ml
Medium
DMEM/F12
Lung
Human

Human

Youk, J. , Kim, T. , Evans, K. , Jeong, Y. , Hur, Y. , Hong, S. , Kim, J. , Yi, K. , Kim, S. , Na, K. , Bleazard, T. , Kim, H. , Fellows, M. , Mahbubani, K. , Saeb-Parsy, K. , Kim, S. , Kim, Y. , Koh, G. , Choi, B. , Ju, Y. and Lee, J.
Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2. , Cell Stem Cell Vol. 27 , 905-919.e10 , 2020
Cells
Aveolar
Enzymes
Medium
DMEM
Lung
Human
Human
Bahmed, K. , Boukhenouna, S. , Karim, L. , Andrews, T. , Lin, J. , Powers, R. , Wilson, M. , Lin, C. , Messier, E. , Reisdorph, N. , Powell, R. , Tang, H. , Mason, R. , Criner, G. and Kosmider, B.
The Effect of Cysteine Oxidation on DJ-1 Cytoprotective Function in Human Alveolar Type II Cells. ,
Cell Death Dis
 10 , 638 , 2019
Cells
Aveolar
Enzymes
Elastase : 12.9 u/ml
Medium
PBS
Lung
Human
Human
Quatromoni, J. , Singhal, S. , Bhojnagarwala, P. , Hancock, W. , Albelda, S. and Eruslanov, E.
An Optimized Dissagregation Method for Human Lung Tumors That Perserves the Phenotype and Function of the Immune Cells ,
J Leukoc Biol
 97 , 201 , 2015
Cells
Lung tumor
Enzymes
Collagenase Type 1 : 45-60 u/ml
Collagenase Type 2 : 15-20 u/ml
Collagenase Type 4 : 45-60 u/ml
Elastase : 0.002%
Medium
DMEM/F12
Lung
Human
Human
Barkauskas, C. , Cronce, M. , Rackley, C. , Bowie, E. , Keene, D. , Stripp, B. , Randell, S. , Noble, P. and Hogan, B.
Type 2 Alveolar Cells are Stem Cells in Adult Lung ,
J Clin Invest
 123 , 3025 , 2013
Cells
Alveolar epithelial
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 0.25%
Elastase : 10 u/ml
Medium
Bronchial Epithelial Growth Medium
Lung
Human
Human
Mackay, L. , Dodd, S. , Dougall, I. , Tomlinson, W. , Lordan, J. , Fisher, A. and Corris, P.
Isolation and Characterisation of Human Pulmonary Microvascular Endothelial Cells from Patients with Severe Emphysema. ,
Respir Res
 14 , 23 , 2013
Cells
Pulmonary endothelial
Enzymes
Medium
RPMI

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.