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Tissue References Search Tool

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Tumor
Human
Human
Varnat, F. , Duquet, A. , Malerba, M. , Zbinden, M. , Mas, C. , Gervaz, P. and Ruiz i Altaba, A.
Human Colon Cancer Epithelial Cells Harbour Active HEDGEHOG-GLI Signalling that is Essential for Tumour Growth, Recurrence, Metastasis and Stem Cell Survival and Expansion. ,
EMBO Mol Med
 1 , 338-51 , 2009
Cells
Colon cancer
Enzymes
Collagenase Type 1 : 300 u/ml
Hyaluronidase : 100 u/ml
Medium
DMEM/F12
Tumor
Human
Human
Kim, M. , Evans, D. , Wang, H. , Abbruzzese, J. , Fleming, J. and Gallick, G.
Generation of Orthotopic and Heterotopic Human Pancreatic Cancer Xenografts in Immunodeficient Mice. ,
Nat Protoc
 4 , 1670 , 2009
Cells
Pancreatic tumor
Enzymes
Collagenase Type 4 : 200 u/ml
Medium
RPMI-1640
Tumor
Human
Human
Liu, R. , Wang, X. , Chen, G. , Dalerba, P. , Gurney, A. , Hoey, T. , Sherlock, G. , Lewicki, J. , Shedden, K. and Clarke, M.
The Prognostic Role of a Gene Signature from Tumorigenic Breast-Cancer Cells. ,
N Engl J Med
 356 , 217 , 2007
Cells
Breast epithelial
Enzymes
Collagenase Type 3 : 200 u/ml
Medium
HBSS
Tumor
Human
Human
Nakashiro Koh-Ichi, Hara Shingo, Shinohara Yuji, Oyasu Miho, Kawamata Hitoshi, Shintani Satoru, Hamakawa Hiroyuki, Oyasu Ryoichi.
Phenotypic switch from paracrine to autocrine role of hepatocyte growth factor in an androgen-independent human prostatic carcinoma cell line, CWR22R ,
Am J Pathol
 165 , 533-40 , 2004
Cells
Prostate stromal cells
Enzymes
Medium
RPMI 1640
Tumor
Human
Human, adult
Nishio Jun, Iwasaki Hiroshi, Ishiguro Masko, Ohjimi Yuko, Fujita Chikako, Isayama Teruto, Naito Masatoshi, Oda Yoshinao, Kaneko Yasuhiko, Kikuchi Masahiro.
Establishment of a new human synovial sarcoma cell line, FU-SY-1, that expresses c-Met receptor and its ligand hepatocyte growth factor ,
Int J Oncol
 21 , 17-23 , 2002
Cells
Human synovial sarcoma
Enzymes
Collagenase Type 2 : 200 u/ml
Medium
DMEM/F-12
Tumor
Human
Human
Emenaker N, Calaf G, Cox D, Basson M. and Qureshi N
Short Chain Fatty Acids Differentially Modulate Cellular Phenotype and c-myc Protein Levels in Primary Human Nonmalignant and Malignant Colonocytes ,
J Nutr
 46 , 96-105 , 2001
Cells
Colonocytes
Enzymes
Collagenase : 0.15%
Medium
DMEM/F12
Tumor
Human
Human
MacLeod, R.
Rapid Monolayer Primary Cell Culture from Tissue Biopsy , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons Ltd , 3E:2.1 , 1995
Cells
Epithelial, fibroblasts
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Tumor
Human
Human
Hague, A. and Paraskeva, C
Colon Adenocarcinoma Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12C:1.1 , 1995
Cells
Colon adenocarcinoma
Enzymes
Hyaluronidase : 100 u/ml
Medium
DMEM
Tumor
Human
Human
Beaupain, R. , Mainquene, C. , Brouty-Boye, D. , Planchon, P. and Magniew, V.
"Normal" Breast Cells Adjacent to a Tumor Grown in Long-term Three Dimensional Culture ,
In Vitro Cell Dev Biol
 29 , 100 , 1993
Cells
Tumor, breast
Enzymes
Hyaluronidase : 100 u/ml
Medium
DMEM
Tumor
Human
Human
Kruse, C. , Mitchell, D. , Kleinschmidt-DeMasteis, B. , Franklin, W. , Morse, H. , Spector, E. and Lillehei, K.
Characterization of a Continuous Human Glioma Cell Line DBTRG-OSMG: Growth Kinetics, Karyotype, Receptor Expression and Tumor Suppressor Gene Analyses ,
In Vitro Cell Dev Biol
 28 , 609 , 1992
Cells
Glioma
Enzymes
Hyaluronidase : 0.01%
Medium
HBSS
Tumor
Human
Human
Emerman, J. and Wilkinson, D.
Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples ,
In Vitro Cell Dev Biol
 26 , 1186 , 1990
Cells
Epithelial
Enzymes
Collagenase : 2.0%
Medium
DMEM/Ham's F-12
Tumor
Human
Human
Boyd, J. , Rinehart Jr. , Walton, L. , Siegal, G. and Kaufman, D.
Ultrastructural Characterization of Two New Human Endometrial Carcinoma Cell Lines and Normal Human Endometrial Epithelial Cells Cultured on Extracellular Matrix ,
In Vitro Cell Dev Biol
 26 , 701 , 1990
Cells
Tumor
Enzymes
Medium
DMEM/Ham's F-12
Tumor
Human
Human, 9-74 year
Sheela S, Riccardi VM, Ratner N.
Angiogenic and invasive properties of neurofibroma Schwann cells ,
J Cell Biol
 111 , 645-53 , 1990
Cells
Neurofibroma
Enzymes
Medium
L-15
Tumor
Human
Human
Sacks, P. , Parnes, S. , Gallick, G. , Mansouri, Z. , Lichtner, R. , Satya-Prakash, K. , Pathak, S. and Parsons, D.
Establishment and Characterization of Two New Squamous Cell Carcinoma Cell Lines Derived from Tumors of the Head and Neck ,
Cancer Res
 48 , 2858 , 1988
Cells
Tumor
Enzymes
Trypsin : 0.05%
Medium
DMEM
Tumor
Human
Human
Brattain, M. , Marks, M. , McCombs, J. , Finely, W. and Brattain, D.
Characterization of Human Colon Carcinoma Cell Lines Isolated From a Single Primary Tumour ,
Br J Cancer
 47 , 373 , 1983
Cells
Tumor, colon
Enzymes
Trypsin : 0.25%
Medium
McCoy's
Tumor
Human
Human
Friedman, E. , Higgins, P. , Lipkin, M. , Shinya, H. and Gelb, A.
Tissue Culture of Human Epithelial Cells from Benign Colonic Tumors ,
In Vitro
 17 , 632 , 1981
Cells
Epthelial and tumor
Colon
Enzymes
Collagenase : 300 u/ml
Medium
PBS medium 199 or medium F 12
Tumor
Human
Human
Leung, C. and Shiu, R.
Morphological and Proliferative Characteristics of Human Breast Tumor Cells Cultured on Plastic and in Collagen Matrix ,
In Vitro
 18 , 476 , 1981
Cells
Tumor, breast
Enzymes
Neuraminidase : 0.8 u/ml
Medium
HBSS
Tumor
Human
Human
Creasey, A. , Smith, H. , Hackett, A. , Fukuyama, K. , Epstein, W. and Madin, S.
Biological Properties of Human Melanoma Cells in Culture ,
In Vitro
 15 , 342 , 1979
Cells
Melanoma
Metastatic tumors
Enzymes
Medium
DMEM
Tumor
Human
Human
Lasfargues, E.
Human Mammary Tumors , Tissue Culture Methods/Applications , Kruse, P., and Patterson, M. , Academic Press , 45 , 1973
Cells
Mammary tumors, hard
Enzymes
Collagenase : 0.10%
Medium
RPMI-1640 w/ 5% Fetal Calf Serum
Tumor
Mouse

Mouse

Nahar, S. , Huang, Y. , Nagy, B. , Zebala, J. , Maeda, D. , Rudloff, U. , Oppenheim, J. and And, D
Regression and Eradication of Triple-Negative Breast Carcinoma in 4T1 Mouse Model by Combination Immunotherapies. , Cancers (Basel) Vol. 15 , 2023
Cells
Tumor
Enzymes
Tumor
Mouse
Mouse
Walker, S. , Wankell, M. , Ho, V. , White, R. , Deo, N. , Devine, C. , Dewdney, B. , Bhathal, P. , Govaere, O. , Roskams, T. , Qiao, L. , George, J. and Hebbard, L.
Targeting mTOR and Src Restricts Hepatocellular Carcinoma Growth in a Novel Murine Liver Cancer Model. ,
PLoS ONE
 14 , e0212860 , 2019
Cells
liver tumor
Enzymes
Medium
HBSS
Tumor
Mouse
Mouse, adult
Neal, J,, Li ,X. , Zhu, J. , Giangarra, V. , Grzeskowiak, C. , Ju, J. , Liu, I. Chiou, S. , Salahudeen, A. and Smith, A. et al
Organoid Modeling of the Tumor Immune Microenvironment. ,
Cell
 175 , 1972 , 2018
Cells
Organoids
Enzymes
Collagenase Type 4 : 200-300 u/ml
Medium
DMEM
Tumor
Mouse
Mouse
Erdreich-Epstein, A. , Singh, A. , Joshi, S. , Vega, F. , Guo, P. , Xu, J. , Groshen, S. , Ye, W. , Millard, M. , Campan, M. , Morales, G. , Garlich, J. , Laird, P. , Seeger, R. , Shimada, H. and Durden, D.
Association of High Microvessel A,B and Low PTEN with Poor Outcome in Stage 3 Neuroblastoma: Rationale for Using First in Class Dual PI3K/BRD4 Inhibitor, SF1126. ,
Oncotarget
 8 , 52193-52210 , 2017
Cells
Tumor derived neutroblastoma
Enzymes
Papain : 10 u/ml
Deoxyribonuclease I : 250 u/ml
Medium
DPBS
Tumor
Mouse
Mouse
Boyd, N. , Walker, K. , Fried, J. , Hackney, J. , McDonald, P. , Benavides, G. , Spina, R. , Audia, A. , Scott, S. , Libby, C. , Tran, A. , Bevensee, M. , Griguer, C. , Nozell, S. , Gillespie, G. and Nabors, B.
Addition of Carbonic Anhydrase 9 Inhibitor SLC-0111 to Temozolomide Treatment Delays Glioblastoma Growth In Vivo. ,
JCI Insight
 2 , 2017
Cells
Glioblastoma
Enzymes
PDS kit : per instructions
Medium
DMEM/F12
Tumor
Mouse
Mouse
Aguilera, T. , Rafat, M. , Castellini, L. , Shehade, H. , Kariolis, M. , Hui, A. , Stehr, H. , von Eyben, R. , Jiang, D. , Ellies, L. , Koong, A. , Diehn, M. , Rankin, E. Graves, E. and Giaccia, A.
Reprogramming the Immunological Microenvironment through Radiation and Targeting Axl. ,
Nat Commun
 7 , 13898 , 2016
Cells
Tumor
Enzymes
Collagenase : 0.1%
Neutral Protease : 0.5 u/ml
Medium
F12K
Tumor
Mouse
Mouse
Otvos, B. , Silver, D. , Mulkearns-Hubert, E. , Alvarado, A. , Turaga, S. , Sorensen, M. , Rayman, P. , Flavahan, W. , Hale, J. , Stoltz, K. , Sinyuk, M. , Wu, Q. , Jarrar, A. , Kim, S. , Fox, P. , Nakano, I. and Rich, J.
Cancer Stem Cell-Secreted Macrophage Migration Inhibitory Factor Stimulates Myeloid Derived Suppressor Cell Function and Facilitates Glioblastoma Immune Evasion. ,
Stem Cells
 34 , 2026-39 , 2016
Cells
Glioblastoma
Enzymes
PDS kit : per instructions
Medium
Neurobasal
Tumor
Mouse
Mouse
Filtjens, J. , Keirsse, J. , Van Ammel, E. , Taveirne, S. , Van Acker, A. , Kerre, T. , Taghon, T. , Vandekerckhove, B. , Plum, J. , Van Ginderachter, J. and Leclercq, G.
Expression of the Inhibitory Ly49E Receptor is Not Critically Involved in the Immune Response Against Cutaneous, Pulmonary or Liver tumours. ,
Sci Rep
 6 , 30564 , 2016
Cells
Tumor
Enzymes
Medium
RPMI-1640
Tumor
Mouse
Mouse
Singer, M. , Wang, C. , Cong, L. , Marjanovic, N. , Kowalczyk, Mo. , Zhang, H. , Nyman, J. , Sakuishi, K. , Kurtulus, S. , Gennert, D. , Xia, J. , Kwon, J. , Nevin, J. , Herbst, R. , Yanai, I. , Rozenblatt-Rosen, O. and Kuchroo, V.
A Distinct Gene Module for Dysfunction Uncoupled from Activation in Tumor-Infiltrating T Cells. ,
Cell
 166 , 1500-1511.e9 , 2016
Cells
Tumor-infiltrating lymphocytes
Enzymes
Medium
DMEM
Tumor
Mouse
Mouse
Hosseini, H. , Obradovic, M. , Hoffmann, M. , Harper, K. , Sosa, M. , Werner-Klein, M. , Nanduri, L. , Werno, C. , Ehrl, C. , Maneck, M. , Patwary, N. , Haunschild, G. , Guzvic, M. and Reimelt, C. et al.
Early Dissemination Seeds Metastasis in Breast Cancer. ,
Nature
 , 2016
Cells
Mammary tumor
Enzymes
Collagenase Type 1 : 200 u/ml
Hyaluronidase : 0.001%
Medium
DMEM/F12
Tumor
Mouse
Mouse
Blanco, S. , Bandiera, R. , Popis, M. , Hussain, S. , Lombard, P. , Aleksic, J. , Sajini, A. , Tanna, H. , Cortes-Garrido, R. , Gkatza, N. , Dietmann, S. and Frye, M.
Stem Cell Function and Stress Response are Controlled by Protein Synthesis. ,
Nature
 534 , 335-40 , 2016
Cells
Keratinocytes
Enzymes
Medium
Low calcium containing media
Tumor
Mouse
Mouse
Kitajima, S. , Kohno, S. , Kondoh, A. , Sasaki, N. , Nishimoto, Y. , Li, F. , Mohammed, M. , Muranaka, H. , Nagatani, N. , Suzuki, M. , Kido, Y. and Takahashi, C.
Undifferentiated State Induced by Rb-p53 Double Inactivation in Mouse Thyroid Neuroendocrine Cells and Embryonic Fibroblasts. ,
Stem Cells
 33 , 1657-69 , 2015
Cells
Thyroid tumor
Enzymes
Collagenase Type 1 : 300 u/ml
Hyaluronidase : 100 u/ml
Medium
DMEM
Tumor
Mouse
Mouse
Xiao, L. , McCann, J. and Dudley, A.
Isolation and Culture Expansion of Tumor-specific Endothelial Cells. ,
J Vis Exp
 , e53072 , 2015
Cells
Endothelial
Enzymes
Medium
DMEM
Tumor
Mouse
Mouse
Ali, O. , Verbeke, C. , Johnson, C. , Sands, R. , Lewin, S. , White, D. , Doherty, E. , Dranoff, G. and Mooney, D.
Identification of Immune Factors Regulating Antitumor Immunity Using Polymeric Vaccines with Multiple Adjuvants. ,
Cancer Res
 74 , 1670-81 , 2014
Cells
Tumor
Enzymes
Collagenase Type 1 : 250 u/ml
Medium
DMEM
Tumor
Mouse
Mouse
Devaud, C. , Westwood, J. , John, L. , Flynn, J. , Paquet-Fifield, S. , Duong, C. , Yong, C. , Pegram, H. , Stacker, S. , Achen, M. Stewart, T. , Snyder, L. , Teng, M. , Smyth, M. , Darcy, P. and Kershaw, M
Tissues in Different Anatomical Sites can Sculpt and Vary the Tumor Microenvironment to Affect Responses to Therapy. ,
Mol Ther
 22 , 18-27 , 2014
Cells
Tumor
Enzymes
Medium
PBS
Tumor
Mouse
Mouse
Dunleavey, J. , Xiao, L. , Thompson, J. , Kim, M. , Shields, J. , Shelton, S. , Irvin, D. , Brings, V. , Ollila, D. , Brekken, R. , Dayton, P. , Melero-Martin, J. and Dudley, A.
Vascular Channels Formed by Subpopulations of PECAM1+ Melanoma Cells. ,
Nat Commun
 5 , 5200 , 2014
Cells
Tumor
Enzymes
Medium
DMEM
Tumor
Mouse
Mouse
Liu, X. , Johnson, S. , Liu, S. , Kanojia, D. , Yue, W. , Singh, U. , Wang, Q, Wang Qi, Nie, Q. and Chen H.
Nonlinear Growth Kinetics of Breast Cancer Stem Cells: Implications for Cancer Stem Cell Targeted Therapy. ,
Sci Rep
 3 , 2473 , 2013
Cells
Mammary tumor
Enzymes
Collagenase : 0.15%
Hyaluronidase : 0.020%
Medium
DMEM/F12

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.