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Tissue References Search Tool

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Muscle
Mouse
Mouse, 6-8 week
Sachdeva, J. , Mahajan, A. , Cheng, J. , Baeten, J. , Lilly, B. , Kuivaniemi, H. and Hans, C.
Smooth Muscle Cell-Specific Notch1 Haploinsufficiency Restricts the Progression of Abdominal Aortic Aneurysm by Modulating CTGF Expression. ,
PLoS ONE
 12 , e0178538 , 2017
Cells
vascular smooth muscle
Enzymes
Elastase : 0.75 u/ml
Medium
DMEM/F12
Muscle
Mouse
Mouse, GFP transgenic
Birbrair, A, Sattiraju, A, Zhu, D, Zulato, G, Batista, I, Nguyen, V, Messi M, Solingapuram Sai, K, Marini, F, Delbono, O. and Mintz, A.
Novel Peripherally Derived Neural-Like Stem Cells as Therapeutic Carriers for Treating Glioblastomas. ,
Stem Cells Transl Med
 , 2016
Cells
Muscle derived NLSC
Enzymes
Medium
DMEM
Muscle
Mouse
Mouse, 2-4 month
Randolph, M. , Phillips, B. , Choo, H. , Vest, K. , Vera, Y. and Pavlath, G.
Pharyngeal Satellite Cells Undergo Myogenesis Under Basal Conditions and Are Required for Pharyngeal Muscle Maintenance. ,
Stem Cells
 33 , 3581-95 , 2015
Cells
Pharyngeal myofiber
Enzymes
Collagenase Type 1 : 400-800 u/ml
Medium
DMEM
Muscle
Mouse
Mouse, 2 month
Abou-Khalil, R. , Yang, F. , Lieu, S. , Julien, A. , Perry, J. , Pereira, C. , Relaix, F. , Miclau, T. , Marcucio, R. and Colnot, C.
Role of Muscle Stem Cells During Skeletal Regeneration. ,
Stem Cells
 33 , 1501-11 , 2015
Cells
Myoblasts
Enzymes
Collagenase Type 2 : 400 u/ml
Neutral Protease : 0.5 u/ml
Medium
Hams F-10
Muscle
Mouse
Mouse, 6-8 week
Pasut, A. , Jones, A. and Rudnicki, M.
Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle. ,
J Vis Exp
 73 , e50074 , 2013
Cells
Myofibers
Enzymes
Medium
DMEM
Muscle
Mouse
Mouse, 10 week
Rezk, B. , Yoshida, T. , Semprun-Prieto, L. , Higashi, Y. , Sukhanov, S. and Delafontaine, P.
Angiotensin II Infusion Induces Marked Diaphragmatic Skeletal Muscle Atrophy. ,
PLoS ONE
 7 , e30276 , 2012
Cells
Diaphragm cells
Enzymes
Collagenase Type 2 : 100 u/ml
Pronase : 0.125%
Medium
PBS
Muscle
Mouse
Mouse
Weisleder, N. , Zhou, J. and Ma, J.
Detection of Calcium Sparks in Intact and Permeabilized Skeletal Muscle Fibers. ,
Methods Mol Biol
 798 , 395 , 2012
Cells
Skeletal muscle fiber
Enzymes
Medium
Tyrode
Muscle
Mouse
Mouse
Metz, R. , Patterson, J. and Wilson, E.
Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization. ,
Methods Mol Biol
 843 , 169 , 2012
Cells
Vascular smooth muscle
Enzymes
Medium
Basal Culture
Muscle
Mouse
Mouse
Shi, H. , Boadu, E. , Mercan, F. , Le, A. , Roth Flach, R. , Zhang, L. , Tyner, K. , Olwin, B. and Bennett, A.
MAP Kinase Phosphatase-1 Deficiency Impairs Skeletal Muscle Regeneration and Exacerbates Muscular Dystrophy. ,
FASEB J
 24 , 2985 , 2010
Cells
Myoblast
Enzymes
Medium
DMEM
Muscle
Mouse
Mouse, 1-25 month
Ieronimakis Nicholas, Balasundaram Gayathri, Reyes Morayma.
Direct isolation, culture and transplant of mouse skeletal muscle derived endothelial cells with angiogenic potential ,
PLoS ONE
 3 , e0001753 , 2008
Cells
Myocytes, endothelial
Enzymes
Medium
PBS
Muscle
Mouse
Mouse, neonatal
Johnson, B. , Scheuer, T. and Catterall, W.
Convergent Regulation of Skeletal Muscle Ca2+ Channels by Dystrophin, the Actin Cytoskeleton, and cAMP-Dependent Protein Kinase. ,
Proc Natl Acad Sci U S A
 102 , 4191 , 2005
Cells
Skeletal muscle myotubes
Enzymes
NCIS kit : per instructions
Medium
L-15
Muscle
Mouse
Mouse, male, 6-14 week
Winitsky, S. , Gopal, T. , Hassanzadeh, S. , Takahashi, H. , Gryder, D. , Rogawski, M. , Takeda, K. , Yu, Z. , Xu, Y. and Epstein, N.
Adult Murine Skeletal Muscle Contains Cells That Can Differentiate into Beating Cardiomyocytes In Vitro. ,
PLoS Biol
 3 , e87 , 2005
Cells
Precursor cells
Enzymes
Medium
DMEM/F12
Muscle
Mouse
Mouse, 15-20 day
Li CX, Liu BH, Tong WD, Zhang LY. and Jiang YP
Dissociation, culture and morphologic changes of interstitial cells of Cajal in vitro ,
World J Gastroenterol
 11 , 2838 , 2005
Cells
Intersitial cells of Cajal
Enzymes
Medium
M199
Muscle
Mouse
Mouse, 6-8 week
Majka S. , Jackson K. , Kienstra K. , Majesky M. , Goodell M. and Hirschi K.
Distinct Progenitor Populations in Skeletal Muscle Are Bone Marrow Derived and Exhibit Different Cell Fates During Vascular Regeneration ,
J Clin Invest
 111 , 71 , 2003
Cells
Skeletal muscle progenitor
Enzymes
Medium
DMEM
Muscle
Mouse
Mouse, 6-8 week
McKinney-Freeman SL, Jackson KA, Camargo FD, Ferrari G, Mavilio F, Goodell MA.
Muscle-derived hematopoietic stem cells are hematopoietic in origin ,
Proc Natl Acad Sci U S A
 99 , 1341 , 2002
Cells
Myocytes
Enzymes
Trypsin : 0.25%
Medium
HBSS
Muscle
Mouse
Mouse, neonatal
Fukada S. , Miyagoe-Suzuki Y. , Tsukihara H. , Yuasa K. , Higuchi S. , Ono S. , Tsujikawa K. , Takeda S. and Yamamoto H.
Muscle Regeneration by Reconstitution with Bone Marrow or Fetal Liver Cells from Green Fluorescent Protein-gene Transgenic Mice ,
J Cell Sci
 115 , 1285 , 2002
Cells
Myocytes
Enzymes
Medium
DMEM
Muscle
Ovine
Sheep, adult and neonatal
Driska S. , Laudadio R. , Wolfson M. and Shaffer T.
A Method for Isolating Adult and Neonatal Airway Smooth Muscle Cells and Measuring Shortening Velocity ,
J Appl Physiol
 86(1) , 427 , 1999
Cells
Tracheal smooth muscle cells
Enzymes
Papain : 0.2%
Medium
MOPS-PSS
Muscle
Porcine
Porcine, postnatal
Miersch, C. , Stange, K. and Rontgen, M.
Effects of Trypsinization and of a Combined Trypsin, Collagenase, and DNase Digestion on Liberation and in Vitro Function of Satellite Cells Isolated from Juvenile Porcine Muscles. ,
In Vitro Cell Dev Biol Anim
 54 , 406-412 , 2018
Cells
Muscle satellite
Enzymes
Medium
Eagle MEM
Muscle
Porcine
Porcine, 2 month
Lewis, F. , Henning, B. , Marazzi, G. , Sassoon, D. , Ellison, G. and Nadal-Ginard, B.
Porcine Skeletal Muscle-Derived Multipotent PW1pos/Pax7neg Interstitial Cells: Isolation, Characterization, and Long-Term Culture. ,
Stem Cells Transl Med
 3 , 702-12 , 2014
Cells
Skeletal muscle
Enzymes
Medium
HBSS
Muscle
Porcine
Porcine, male, 25-40 kg
Korzick, D. , Laughlin, M. and Bowles, D.
Alterations in PKC signaling underlie enhanced myogenic tone in exercise-trained porcine coronary resistance arteries ,
J Appl Physiol
 96 , 1425-32 , 2004
Cells
Coronary myocytes
Enzymes
Medium
low calcium physiological saline
Muscle
Porcine
Porcine, adult, 35-45 kg
Wamhoff BR, Dixon JL, Sturek M.
Atorvastatin treatment prevents alterations in coronary smooth muscle nuclear Ca2+ signaling in diabetic dyslipidemia ,
J Vasc Res
 39 , 208 , 2002
Cells
Arterial smooth muscle
Enzymes
Collagenase Type 2 : 294 u/ml
Elastase : 6.5 u/ml
Deoxyribonuclease I : 0.4 mg/ml
Medium
MEM
Muscle
Porcine
Porcine, Yorkshire, 30kg
Sirous ZN, Fleming JB, Khalil RA.
Endothelin-1 enhances eicosanoids-induced coronary smooth muscle contraction by activating specific protein kinase C isoforms ,
Hypertension
 37 , 497-504 , 2001
Cells
Coronary smooth muscle
Enzymes
Collagenase Type 2 : 150 u/ml
Elastase : 0.05%
Medium
HBSS
Muscle
Porcine
Porcine
Shieh CC, Feng J, Buckner SA, Brioni JD, Coghlan MJ, Sullivan JP, Gopalakrishnan M.
Functional implication of spare ATP-sensitive K(+) channels in bladder smooth muscle cells ,
J Pharmacol Exp Ther
 296 , 669-75 , 2001
Cells
Bladder smooth muscle
Enzymes
Collagenase Type 2 : 0.1-0.2%
Medium
Krebs-Ringer bicarbonate
Muscle
Porcine
Porcine, juvenile, 30kg
Huckle WR, Drag MD, Acker WR, Powers M, McFall RC, Holder DJ, Fujita T, Stabilito II, Kim D, Ondeyka DL, Mantlo NB, Chang RS, Reilly CF, Schwartz RS, Greenlee WJ, Johnson RG.
Effects of subtype-selective and balanced angiotensin II receptor antagonists in a porcine coronary artery model of vascular restenosis ,
Circulation
 93 , 1009-19 , 1996
Cells
Coronary smooth muscle cells
Enzymes
Collagenase : 0.3%
Elastase : 0.05%
Medium
HBSS
Muscle
Porcine
Porcine
Xiong, Yimin, Xu, Shangzhe. and Slakey, Linda L
Modulation of Response to Adenosine in Vascular Smooth Muscle Cells Cultured in Defined Medium ,
In Vitro Cell Dev Biol
 27 , 355 , 1991
Cells
Smooth muscle, aorta
Enzymes
Collagenase : 0.3%
Medium
DMEM
Muscle
Porcine
Porcine
Breton, M. , Berrou, E. , Deudon, E. and Picard, J.
Changes in Proteoglycans of Cultured Pig Aortic Smooth Muscle Cells During Subculture ,
In Vitro Cell Dev Biol
 26 , 157 , 1990
Cells
Smooth muscle
Aorta
Enzymes
Trypsin : 0.05%
Medium
EDTA 0.02%
Muscle
Porcine
Porcine
Fehr, T. , Dickinson, E. , Goldman, S. and Slakey, L.
Cyclic AMP Efflux is Regulated by Occupancy of The Adenosine Receptor in Pig Aortic Smooth Muscle Cells ,
J Biol Chem
 265 , 10974 , 1990
Cells
Smooth muscle
Aortic medial tissue
Enzymes
Collagenase : 0.30%
Medium
DMEM
Muscle
Quail
Quail, embryo, 10 day
Konigsberg, I.
Skeletal Myoblasts in Culture ,
Methods Enzymol
 LVIII , 511 , 1979
Cells
Myoblasts
Enzymes
Medium
Puck's solution
Muscle
Rabbit
Rabbit, New Zealand
Al-Shboul, O. , Mahavadi, S. , Sriwai, W. , Grider, J. and Murthy, K.
Differential Expression of Multidrug Resistance Protein 5 and Phosphodiesterase 5 and Regulation of cGMP Levels in Phasic and Tonic Smooth Muscle. ,
Am J Physiol Gastrointest Liver Physiol
 305 , G314-24 , 2013
Cells
Gastric smooth muscle
Enzymes
Medium
DMEM
Muscle
Rabbit
Rabbit, New Zealand White
Croons Valerie, Martinet Wim, Herman Arnold G, Timmermans Jean-Pierre, De Meyer Guido R Y.
Selective clearance of macrophages in atherosclerotic plaques by the protein synthesis inhibitor cycloheximide ,
J Pharmacol Exp Ther
 320 , 986-93 , 2007
Cells
Aortic smooth muscle
Enzymes
Collagenase Type 2 : 300 u/ml
Elastase : 5 u/ml
Medium
F10 Ham's
Muscle
Rabbit
Rabbit, New Zealand, male, 2.5-3.5 Kg
Santos, M. , Nguyen, B.. and Thompson, J.
Factors Affecting in Vitro Growth of Harvested Enterocytes ,
Cell Transplant
 1 , 299 , 1992
Cells
Enterocytes
Enzymes
Trypsin : 0.1%
Medium
RPMI 1640 w/ 1% fetal bovine serum
PBS
Muscle
Rabbit
Rabbit
(also rat, guinea-pig)
Hu, S. and Kim, H.
Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid ,
FASEB J
 6 , A383 , 1992
Cells
Smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Potassium buffer solution
Muscle
Rabbit
Rabbit, adult, 1-2 kg
Benham, C. , Bolton, T. , Byrne, N. and Large, W.
Action of Extremely Applied Adenosine Triphosphate O Single Smooth Muscle Cells Dispersed From Rabbit Ear Artery ,
J Physiol
 387 , 473 , 1987
Cells
Smooth muscle
Enzymes
Elastase : 0.17 - 0.25%
Medium
Saline
Muscle
Rabbit
Rabbit, New Zealand white, 1500 g
Knodle, S. , Anderson, S. and Papaioannou, S.
Large Scale Preparation of Rabbit Aortic Smooth Muscle Cells For Use in Calcium Uptake Studies ,
In Vitro Cell Dev Biol
 22 , 23 , 1986
Cells
Smooth muscle, aortic
Enzymes
Trypsin : 0.038%
Medium
MEM
Muscle
Rabbit
Rabbit, adult, 1-2 Kg
Benham, C.D. and Bolton, T.B.
Spontaneous Transient Outward Currents in Single Visceral and Vascular Smooth Muscle Cells of the Rabbit ,
J Physiol
 381 , 385 , 1986
Cells
Smooth muscle, ear artery
Enzymes
Trypsin : 0.1%
Medium
CF solution
(see reference)
Muscle
Rabbit
Rabbit, 0.5-1 Kg
(also guinea-pig, frog, dogfish)
Mitra, R. and Morad, M.
A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates ,
Am J Physiol
 249 , H1056 , 1985
Cells
Myocytes, heart and stomach
Enzymes
Protease XIV : 0.028%
Medium
Solution C
(see reference)

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.