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Tissue References Search Tool

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Lung
Rat
Rat, embryonic day 15
Fraslon-Vanhulle, C, Bourbon, J. and Batenburg, J
Culture of Fetal Alveolar Epithelial Type II Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 2 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 13A:2.1 , 1995
Cells
Fetal alveolar epithelial type II
Enzymes
Trypsin : 0.1%
Medium
DMEM
Lung
Rat
Rat, SD, male, 125-175 g
Kim, K. , Suh, D. , Lubman, R. , Danto, S. , Borok, Z. and Crandall, E.
Studies on the Mechanisms of Active Ion Fluxes Across Alveolar Epithelial Cell Monolayers ,
J Tiss Cul Meth
 14 , 187 , 1992
Cells
Alveolar epithelial
Enzymes
Elastase : 2 u/ml
Medium
EBSS
Lung
Rat
Rat, Wistar, female, virgin
Fraslon, C. , Rolland, G. , Bourbon, J. , Rieutort, M. and Valenza, C.
Culture of Fetal Alveolar Epithelial Type II Cells in Serum-Free Medium ,
In Vitro Cell Dev Biol
 27A , 843 , 1991
Cells
Alveolar epithelial type II
Enzymes
Trypsin : 1%
Medium
Eagle's MEM
Lung
Rat
Rat, Wistar
Jassal, D. , Han, R. , Caniggia, I. , Post, M. and Tanswell, A.
Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium ,
In Vitro Cell Dev Biol
 27A , 625 , 1991
Cells
Epithelial
Enzymes
Trypsin : 0.1%
Medium
HBSS
Lung
Rat
Rat, SD, 8 day
Berk, J. , Franzblau, C. and Goldstein, R.
Recombinant Interleukin-1beta Inhibits Elastin Formation by a Neonatal Rat Lung Fibroblast Subtype ,
J Biol Chem
 266 , 3192 , 1991
Cells
Interstitial
Enzymes
Trypsin : 1.125%
Medium
HEPES buffer
Lung
Rat
Rat, Fischer 344, male
Mangum, J. , Everitt, J. , Bonner, J. , Moore, L. and Brody, A.
Co-Culture of Primary Pulmonary Cells to Model Alveolar Injury and Translocation of Proteins ,
In Vitro Cell Dev Biol
 26 , 1135 , 1990
Cells
Alveolar type II pneumocytes
Enzymes
Elastase : 40 u/ml
Medium
HEPES buffer
(see reference)
Lung
Rat
Rat, SD, male, 250-400 g
Ma, J. , LaCagnin, L. , Bowman, L. and Miles, P.
Carbon Tetrachloride Inhibits Synthesis of Pulmonary Surfactant Disaturated Phosphatidylcholines and ATP Production in Alveolar Type II Cells ,
Biochim Biophys Acta
 1003 , 136 , 1989
Cells
Alveolar type II
Enzymes
Elastase : 40 u/ml
Medium
Phosphate-buffered medium
(see reference)
Lung
Rat
Rat, Wistar, adult, male and pregnant female (known gestation)
Batenburg, J. , Otto-Verberne, C. , Have-Opbroek, A. and Klazinga, W.
Isolation of Alveolar Type II Cells from Fetal Rat Lung by Differential Adherence in Monolayer Culture ,
Biochim Biophys Acta
 960 , 441 , 1988
Cells
Alveolar type II
Enzymes
Trypsin : 0.1%
Medium
RPMI 1640
Lung
Rat
Rat, SD, male, 150 - 200 g
Brown, L. and Longmore, W.
Altered Phospholipid Secretion in Type II Pneumocytes Isolated from Streptozotocin-diabetic Rats ,
Biochim Biophys Acta
 878 , 258 , 1986
Cells
Pneumocytes type II
Enzymes
Trypsin : 0.30%
Medium
BSS
Lung
Rat
Rat, SD, male, 180-200 g
Dobbs, L. , Gonzalez R. and Williams, M.
An Improved Method for Isolating Type ll Cells in High Yield and Purity ,
Am Rev Respir Dis
 134 , 141 , 1986
Cells
Alveolar type II
Enzymes
Elastase : 4.3 u/ml
Medium
HEPES
(see reference)
Lung
Rat
Rat, SD, male
Weller, N. and Karnovksy, M.
Isolation of Pulmonary Alveolar Type I Cells From Adult Rats ,
Am J Pathol
 124 , 448 , 1986
Cells
Alveolar type I
Enzymes
Trypsin : 0.05%
Medium
DMEM
Lung
Rat
Rat, fetus, 19 day
Post, M. , Torday, J. and Smith, B.
Alveolar Type II Cells Isolated from Fetal Rat Lung Organotypic Cultures Synthesize and Secrete Surfactant-Associated Phospholipids and Respond to Fibroblast-Pneumonocyte Factor ,
Exp Lung Res
 7 , 53 , 1984
Cells
Fibroblasts & type II
Enzymes
Trypsin : 0.05%
Medium
HBSS: serum free MEM, CMF
Lung
Rat
Rat, SD, male, 150-400 g
Goodman, B. , Fleischer, R. and Crandall, E.
Evidence for Active Na+ Transport by Cultured Monolayers of Pulmonary Alveolar Epithelial Cells ,
Am J Physiol
 245 , C78 , 1983
Cells
Alveolar type II
Enzymes
Elastase : 4 u/ml
Medium
Auto-Pow Eagle's modified MEM
Lung
Rat
Rat, SD, male/female, 180-250 g
Mason, R. , Williams, M. , Greenleaf, R. and Clements, J.
Isolation and Properties of Type II Alveolar Cells from Rat Lung ,
Am Rev Respir Dis
 115 , 1015 , 1977
Cells
Alveolar type II
Enzymes
Trypsin : 0.30%
Medium
BSS
Lung
Rat
Rat, Wistar, pathogen free
King, R.
Metabolic Fate of the Apoproteins of Pulmonary Surfactant ,
Am Rev Respir Dis
 115 , 73 , 1977
Cells
Alveolar type II
Enzymes
Trypsin : 0.50%
Medium
Earle's MEM
Lung
Rat
Rat, fetus, 19 days
Douglas, W. and Teel, R.
An Organotypic in Vitro Model System for Studying Pulmonary Surfactant Production by Type II Alveolar Pneumonocytes ,
Am Rev Respir Dis
 113 , 17 , 1976
Cells
Alveolar pneumonocytes, type II
Enzymes
Trypsin : 0.1 %
Medium
HBSS, CMF
Lung
Rat
Rat, adult
Douglas, W. and Kaighn, M.
Clonal Isolation of Differentiated Rat Lung Cells ,
In Vitro
 10 , 230 , 1974
Cells
Lung
Enzymes
Collagenase : 0.1%
Medium
Moscona saline, CMF
Lung
Rat
Rat, SD, male, 100 g
Kikkawa, Y. and Yoneda, K.
Type II Epithelial Cell of the Lung. I. Method of Isolation ,
Lab Invest
 30 , 76 , 1974
Cells
Alveolar type II
Enzymes
Trypsin : 1.0%
Medium
Joklik's medium
Tumor
Canine

Canine, adult

Petrouškova, P. , Hudáková, N. , Almášiová, V. , Valenčáková, A. , Horňáková, L. , Huniadi, M. and Čížková, D.
Establishment of Primary Cell Cultures from Canine Mammary Gland Malignant Tumours: A Preliminary Study. , J Vet Res Vol. 69 , 159-168 , 2025
Cells
Mammary gland tumor
Enzymes
Medium
EBSS
Tumor
Hamster
Hamster, 90-100 g
Gonzalez, A. , Oberley, T. , Schultz, J. , Ostrom, J. and Li, J.
In Vitro Charactrization of Estrogen Induced Syrian Hamster Renal Tumors: Comparison with an Immortalized Cell Line Derived from Diethylstilbestrol-Treated Adult Hamster Kidney ,
In Vitro Cell Dev Biol
 29A , 562 , 1993
Cells
Tumor
Enzymes
Hyaluronidase : 0.1%
Medium
Waymouth's MB
Tumor
Hamster
Hamster, 6 week old
Min, B. , Kim, K. , Cherrick, H. and Park, N.
Three Cell Lines from Hamster Buccal Pouch Tumors Induced by Topical 7,12-Dimethylbenz(a)Anthracene, Alone or in Conjunction with Herpes Simplex Virus Inoculation ,
In Vitro Cell Dev Biol
 27A , 128 , 1991
Cells
Buccal pouch
Enzymes
Medium
CMF HBSS
Tumor
Human

Human

Song, H. , Weinstein, H. , Allegakoen, P. , Wadsworth, M. , Xie, J. , Yang, H. , Castro, E. , Lu, K. , Stohr, B. , Feng, F. , Carroll, P. , Wang, B. , Cooperberg, M. , Shalek, A. and Huang, F.
Single-cell Analysis of Human Primary Prostate Cancer Reveals the Heterogeneity of Tumor-associated Epithelial Cell States. ,
Nat Commun
 13 , 141 , 2022
Cells
Prostate cells
Enzymes
Collagenase Type 4 : 1,000 u/ml
Medium
RPMI
Tumor
Human
Human
Jeske, S. , Schuler, P. , Doescher, J. , Theodoraki, M. , Laban, S. , Brunner, C. , Hoffman, T. and Wigand, M.
Age-related changes in T lymphocytes of patients with head and neck squamous cell carcinoma ,
Immun Ageing
 17 , 3 , 2020
Cells
Tumor infiltrating lympocytes
Enzymes
Collagenase Type 1 : 200 u/ml
Medium
RPMI
Tumor
Human
Human
Ayuso, J. , Vitek, R. , Swick, A. , Skala, M. , Wisinski, K. , Kimple, R. , Lambert, P. and Beebe, D.
Effects of Culture Method on Response to EGFR Therapy in Head and Neck Squamous Cell Carcinoma Cells ,
Sci Rep
 9 , 12480 , 2019
Cells
Fibroblasts
Enzymes
Medium
FM Fibroblast media
Tumor
Human
Human
Phan, N. , Hong, J. , Tofig, B. , Mapua, M. , Elashoff, D. , Moatamed, N. , Huang, J. , Memarzadeh, S. , Damoiseaux, R. and Soragni, A.
A Simple High-Throughput Approach Identifies Actionable Drug Sensitivities in Patient-Derived Tumor Organoids. ,
Commun Biol
 2 , 78 , 2019
Cells
Tumor
Enzymes
Collagenase Type 4 : 200 u/ml
Medium
DMEM
Tumor
Human
Neal, J,, Li ,X. , Zhu, J. , Giangarra, V. , Grzeskowiak, C. , Ju, J. , Liu, I. Chiou, S. , Salahudeen, A. and Smith, A. et al
Organoid Modeling of the Tumor Immune Microenvironment. ,
Cell
 175 , 1972 , 2018
Cells
Organoids
Enzymes
Collagenase Type 4 : 200-300 u/ml
Medium
DMEM
Tumor
Human
Human
Jiang, C. , Zhang, Q. , Shanti, R. , Shi, S. , Chang, T. , Carrasco, L. , Alawi, F. and Le, A.
Mesenchymal Stromal Cell-Derived Interleukin-6 Promotes Epithelial-Mesenchymal Transition and Acquisition of Epithelial Stem-Like Cell Properties in Ameloblastoma Epithelial Cells. ,
Stem Cells
 35 , 2083-2094 , 2017
Cells
Ameloblastoma epithelial
Enzymes
Collagenase : 0.1%
Neutral Protease : 50 u/ml
Medium
MEM
Tumor
Human
Human, 25-81 yr
Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,
A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
 17 , 30 , 2016
Cells
Brain tumor
Enzymes
Neutral Protease : 0.11 u/ml
Hyaluronidase : 1,000 u/ml
Medium
HBSS
Tumor
Human
Human
Pham, K. , Delitto, D. , Knowlton, A. , Hartlage, E. , Madhavan, R. , Gonzalo, D. , Thomas, R. , Behrns, K. , George, T. , Hughes, S. , Wallet, S. , Liu, C. and Trevino, J.
Isolation of Pancreatic Cancer Cells from a Patient-Derived Xenograft Model Allows for Practical Expansion and Preserved Heterogeneity in Culture. ,
Am J Pathol
 186 , 2016
Cells
Pancreatic cancer
Enzymes
Stemxyme 1 : 0.2%
Medium
HBSS
Tumor
Human
Human
Baldan, V. , Griffiths, R. , Hawkins, R. and Gilham, D
Efficient and Reproducible Generation of Tumour-Infiltrating Lymphocytes for Renal Cell Carcinoma ,
Br J Cancer
 112 , 1510 , 2015
Cells
Tumour infiltrating lymphocytes
Enzymes
Medium
RPMI 1640
Tumor
Human
Human
Ali, M. , Anand, S. , Tangella, K. , Ramkumar, D. and Saif, T.
Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry. ,
J Vis Exp
 , e52532 , 2015
Cells
Colon
Enzymes
Trypsin : 0.1%
Collagenase : 0.1%
Medium
HBSS
Tumor
Human
Human
Liang, S. , Marti, T. , Dorn, P. , Froment, L. , Hall, S. , Berezowska, S. , Kocher, G. , Schmid, R. and Peng, R.
Blocking the Epithelial-to-Mesenchymal Transition Pathway Abrogates Resistance to Anti-Folate Chemotherapy in Lung Cancer. ,
Cell Death Dis
 6 , e1824 , 2015
Cells
Non-small cell lung tumor
Enzymes
Medium
RPMI-1640
Tumor
Human
Human
Quatromoni, J. , Singhal, S. , Bhojnagarwala, P. , Hancock, W. , Albelda, S. and Eruslanov, E.
An Optimized Dissagregation Method for Human Lung Tumors That Perserves the Phenotype and Function of the Immune Cells ,
J Leukoc Biol
 97 , 201 , 2015
Cells
Lung tumor
Enzymes
Collagenase Type 1 : 45-60 u/ml
Collagenase Type 2 : 15-20 u/ml
Collagenase Type 4 : 45-60 u/ml
Elastase : 0.002%
Medium
DMEM/F12
Tumor
Human
Human
Yan, K. , Wu, Q. , Yan, D. , Lee, C. , Rahim, N. , Tritschler, I. , DeVecchio, J. , Kalady, M. , Hjelmeland, A. and Rich, J.
Glioma Cancer Stem Cells Secrete Gremlin1 to Promote their Maintenance Within the Tumor Hierarchy. ,
Genes Dev
 28 , 1085-100 , 2014
Cells
Glioma cancer stem
Enzymes
PDS kit : per instructions
Medium
Neurobasal
Tumor
Human
Human
Hasselbach, L. , Irtenkauf, S. , Lemke, N. , Nelson, K. , Berezovsky, A. , Carlton, E. , Transou, A. , Mikkelsen, T. and deCarvalho, A.
Optimization of High Grade Glioma Cell Culture from Surgical Specimens for Use in Clinically Relevant Animal Models and 3D Immunochemistry. ,
J Vis Exp
 , e51088 , 2014
Cells
Glioblastoma tumor
Enzymes
Trypsin : 0.025%
Collagenase Type 4 : 500 u/ml
Medium
HBSS
Tumor
Human
Human
Dobbin, Z. and Landen, C.
Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors--Potential Applications. ,
Curr Protoc Pharmacol
 63 , Unit 14.28. , 2013
Cells
Cancer stem
Enzymes
Trypsin : 0.25%
Collagenase : 0.05%
Hyaluronidase : 0.005%
Medium
RPMI

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.