For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.

Quick Order

Tissue References Search Tool

Search
Muscle
Rat
Rat, Wistar Kyoto, 10-15 weeks
McGuire, P. , Walker-Caprioglio, H. , Little, S. and McGuffee, L.
Isolation and Culture of Rat Superior Mesenteric Artery Smooth Muscle Cells ,
In Vitro Cell Dev Biol
 29 , 135 , 1993
Cells
Smooth muscle, mesenteric artery
Enzymes
Trypsin : 0.05%
Medium
MEM
Muscle
Rat
Rat
(also rabbit, guinea-pig)
Hu, S. and Kim, H.
Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid ,
FASEB J
 6 , A383 , 1992
Cells
Smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Potassium buffer solution
Muscle
Rat
Rat, SD, 19 days
Loch-Caruso, R. , Pahl, M. and Juberg, D.
Rat Myometrial Smooth Muscle Cells Show High Levels of Gap Junctional Communication Under a Variety of Culture Conditions ,
In Vitro Cell Dev Biol
 28 , 97 , 1992
Cells
Smooth muscle, myometrial
Enzymes
Trypsin : 150 µg/ml
Medium
HBSS or PSS, CMF
Muscle
Rat
Rat, SHRs and WKY, male, 10 - 14 weeks
Bolzon, B. and Cheung, D.
Isolation and Characterization of Single Vascular Smooth Muscle Cells From Spontaneously Hypertensive Rats ,
Hypertension
 14 , 137 , 1989
Cells
Smooth muscle, tail arteries
Enzymes
Papain : 0.1%
Medium
HEPES buffer
(see reference)
Muscle
Rat
Rat, 1-3 day
Barone, L. , Wolfe, L. , Faris, B. and Franzblau, C.
Elastin mRNA Levels and Insoluble Elastin Accumulation in Neonatal Rat ,
Biochemistry
 27 , 3175 , 1988
Cells
Smooth muscle, aortic
Enzymes
Elastase : 0.0125%
Medium
DMEM
Muscle
Rat
Rat
Bean, B. , Sturek, M. , Puga, A. and Hermsmeyer, K.
Calcium Channels in Muscle Cells Isolated From Rat Mesenteric Arteries: Modulation by Dihydropyridine Drugs ,
Circ Res
 59 , 229 , 1986
Cells
Muscle, mesenteric arteries
Enzymes
Trypsin : 0.05%
Medium
HEPES KG solution
(see reference)
Muscle
Rat
Rat, SHR, WKY. either sex, 12-19 day, 3 month, and retired breeders
Gordon, D. , Mohai, L. and Schwartz, S.
Induction of Polyploidy in Cultures of Neonatal Rat Aortic Smooth Muscle Cells ,
Circ Res
 59 , 633 , 1986
Cells
Endothelial, aortic
Enzymes
Elastase : 0.05%
Medium
Waymouth's culture medium
Muscle
Rat
Rat, male, 150-250 g
Cole, O. , Fan, T. and Lewis, G.
Isolation, Characterization, Growth and Culture of Endothelial Cells From the Rat Aorta ,
Cell Biol Int Rep
 10 (6) , 399 , 1986
Cells
Endothelial, aortic
Medium
RPMI 1640
Muscle
Rat
Rat, SD, male, 200 - 250 g
Brock, T. , Alexander, R. , Ekstein, L. , Atkinson, W. and Gimbrone, M.
Angiotensin Increases Cytosolic Free Calcium in Cultured Vascular Smooth Muscle Cells ,
Hypertension
 7 , 105 , 1985
Cells
Smooth muscle, thoracic aorta
Enzymes
Trypsin : 0.0375%
Medium
Eagle's MEM with calcium
Muscle
Rat
Rat, Wistar, female, 10 weeks
Boulanger-Saunier, C. , Kattenburg, D. and Stoclet, J.
Cyclic AMP-dependent Phosphorylation of a 16kDa Protein in a Plasma Membrane-enriched Fraction of Rat Aortic Myocytes ,
F.E.B.S. Lett.
 193 , 283 , 1985
Cells
Myocytes
Enzymes
Collagenase : 0.1%
Medium
HBSS
Muscle
Rat
Rat, SD, 225-250g
Gunther S, Alexander RW, Atkinson WJ. and Gimbrone MA Jr.
Functional angiotensin II receptors in cultured vascular smooth muscle cells ,
J Cell Biol
 92 , 289 , 1982
Cells
Mesenteric artery smooth muscle cells
Enzymes
Medium
HBSS
Muscle
Rat
Rat, 3-4 day
Kasten, F.
Rat Myocardial Cells In Vitro: Mitosis and Differentiated Properties ,
In Vitro
 8 , 128 , 1972
Cells
Myocardial
Enzymes
Trypsin NF 1:250 : 0.125%
Medium
HBSS CMF
Muscle
Rat
Rat, 200 g
Kono, T.
Roles of Collagenases and Other Proteolytic Enzymes in the Dispersal of Animal Tissues ,
Biochim Biophys Acta
 178 , 397 , 1969
Cells
Muscle
Enzymes
Trypsin : 0.05%
Medium
Kreb's Henseleit bicarbonate buffer
Muscle
Rat
Rat, Wistar, 3-10 day
Harary, I. and Farley, B.
In Vitro Studies on Single Beating Rat Heart Cells ,
Exp Cell Res
 29 , 451 , 1963
Cells
Heart
Enzymes
Trypsin NF 1:250 : 250: 0.1%
Medium
Saline A
(See reference)
Thymus
Human
Human
Vandenabeele S, Hochrein H, Mavaddat N, Winkel K, Shortman K.
Human thymus contains 2 distinct dendritic cell populations ,
Blood
 97 , 1733-41 , 2001
Cells
Dentritic
Enzymes
Medium
RPMI 1640
Thymus
Mouse
Mouse
Rode, I. and Boehm, T.
Regenerative Capacity of Adult Cortical Thymic Epithelial Cells. ,
Proc Natl Acad Sci U S A
 109 , 3463-8 , 2012
Cells
Thymic stromal
Enzymes
Medium
RPMI-1640
Thymus
Mouse
Mouse
Phillips, Joy, Brondstetter, T. , English, C. , Lee, H. , Virts, E. and Thoman, M.
IL-7 Gene Therapy in Aging Restores Early thymopoiesis without Reversing Involution ,
J Immunol
 , 4869 , 2004
Cells
Stromal
Enzymes
Medium
RPMI 1640
Thymus
Mouse
Mouse
Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F. and Gabrieli, L
ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells ,
J Exp Med
 196 , 1415 , 2002
Cells
Dentritic
Enzymes
Medium
RPMI 1640
Thymus
Mouse
Mouse, 3-6 week
Smith KM, Olson DC, Hirose R, Hanahan D.
Pancreatic gene expression in rare cells of thymic medulla: evidence for functional contribution to T cell tolerance ,
Int Immunol
 9 , 1355-65 , 1997
Cells
Thymic
Enzymes
Collagenase Type 3 : 100-400 u/ml
Medium
HBSS
Thymus
Mouse
Mouse (BALB/c, C3H, C57BL/6), 1-28 day
Ropke, C. , van Deurs, B. and Petersen, O.
Short-term Cultivation of Murine Thymic Epithelial Cells in a Serum-Free Medium ,
In Vitro Cell Dev Biol
 26 , 671 , 1990
Cells
Epithelial, thymus
Enzymes
Neutral Protease : 1.5 µg/ml
Medium
DMEM
Thymus
Mouse
Mouse, C3H, 16 wk old, female
Ehmann, U. , Shiurba, R. and Peterson, W.
Long-Term Proliferation of Mouse Thymic Epithelial Cells in Culture ,
In Vitro Cell Dev Biol
 22 (12) , 738 , 1986
Cells
Epithelial
Enzymes
Medium
DMEM
Thymus
Mouse
Mouse, Swiss, 6 wk
Jones, K. and Pierre, R.
Analysis of Cellular Heterogeneity in Mouse Thymus Cultures ,
In Vitro
 17 , 431 , 1981
Cells
Thymus
Enzymes
Collagenase Type 3 : 150 u/ml
Medium
DMEM
Thymus
Rat
Rat, postnatal
Bonfanti, P. , Claudinot, S. , Amici, A. , Farley, A. , Blackburn, C. and Barrandon, Y.
Microenvironmental Reprogramming of Thymic Epithelial Cells to Skin Multipotent Stem Cells. ,
Nature
 466 , 978 , 2010
Cells
Thymic
Enzymes
Trypsin : 0.05%
Medium
HBSS
Thymus
Rat
Rat
Masuda, A. and Matsuyama, M
Epithelial Cell Lines From Rat Thymoma and Rat Thymus , Cell & Tissue Culture: Laboratory Procedures Vol. 2 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 21C:4.1 , 1995
Cells
Thymic epithelial
Enzymes
Medium
DMEM
Thymus
Rat
Rat (ACI/NMs X BUF/Mna) F1, male, 28 months
Rat, (ACI/MNs) male, 8 weeks
(also bovine, adult)
Masuda, A. , Ohtsuka, K. and Matsuyama, M.
Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus ,
In Vitro Cell Dev Biol
 26 , 713 , 1990
Cells
Epthelial
Enzymes
Medium
Eagle's MEM Serum-free

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.