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Pancreas
Rat
Rat, SD, male, 40 - 100 g
Hirschi, K. , Kenny, S. , Justice, J. and Brannon, P.
Effects of Secretin And Caerulein On Enzymes Of Cultured Pancreatic Acinar Cells ,
In Vitro Cell Dev Biol
 27 , 660 , 1991
Cells
Acinar
Enzymes
Hyaluronidase : 462 u/ml
Medium
Ham's F12
Pancreas
Rat
Rat, SD, male, 100 - 150 g
Yeh, C. , Mertz, P. , Oliver, C. , Baum, B. and Kousvelari, E.
Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells ,
In Vitro Cell Dev Biol
 27 , 707 , 1991
Cells
Parotid acinar
Enzymes
Trypsin : 0.001%
Medium
F12 medium
Pancreas
Rat
Rat, Wistar, male
Foskett, J. , Roifman, C. and Wong, D.
Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells ,
J Biol Chem
 266 , 2778 , 1991
Cells
Parotid acinar
Enzymes
Trypsin : 0.02%
Medium
Solution B
(See reference)
Pancreas
Rat
Rat, SD, both sexes, 6-20 wks old
Githens, S. , Schexnayder, J. , Desai, K. and Patke, C.
Rat Pancreatic Interlobular Duct Epithelium: Isolation and Culture in Collagen Gel ,
In Vitro Cell Dev Biol
 25 (8) , 679 , 1989
Cells
Interlobular ducts
Enzymes
Papain : 25 u/ml
Medium
DMEM/Ham's F-12
Pancreas
Rat
Rat, SD, male, 150 - 200 g
Menozzi, D. , Sato, S. , Jensen, R. and Gardner, J.
Cyclic GMP Does Not Inhibit Protein Kinase C-Mediated Enzyme Secretion in Rat Pancreatic Acini ,
J Biol Chem
 264 , 995 , 1989
Cells
Acinar
Enzymes
Medium
HEPES
Pancreas
Rat
Rat, Wistar, male, 250 - 350 g
Melvin, J. , Kawaguchi, M. , Baum, B. and Turner, R.
A Muscarinic Agonist-Stimulated Chloride Efflux Pathway is Associated With Fluid Secretion in Rat Parotid Acinar Cells ,
Biochem Biophys Res Commun
 145 , 754 , 1987
Cells
Acinar, parotid
Enzymes
Hyaluronidase : 0.015%
Medium
Earle's MEM
Pancreas
Rat
Rat, Fischer-344, either sex, 120-150 g
Tsao, M. and Duguid, W.
Establishment of Propagable Epithelial Cell Lines From Normal Adult Rat Pancreas ,
Exp Cell Res
 168 , 365 , 1987
Cells
Epithelial
Enzymes
Trypsin : 0.1%
Medium
Ham's F-12/HBSS
(see reference)
Pancreas
Rat
Rat, SD, male, 50 - 125 g
Oliver, C. , Waters, J. , Tolbert, C. and Kleinman, H.
Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel ,
In Vitro Cell Dev Biol
 23 , 465 , 1987
Cells
Acinar
Exorbital lacrimal, parotid, pancreas
Enzymes
Trypsin : 0.01%
Medium
HBSS, CMF
Pancreas
Rat
Rat, 200-350 g
Gotoh, M. , Maki, T. , Satomi, S. , Porter, J. , Bonner-Weir, S. , O'Hara, C. and Monaco, A.
Reproducible High Yield of Rat Islets By Stationary In Vitro Digestion Following Pancreatic Ductal or Portal Venous Collagenase Injection ,
Transplantation
 43 (5) , 725 , 1987
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Rat
Rat, SD, male, 42 - 48 day, 175 - 200 g
Quissell, D. , Redman, R. and Mark, M.
Short-Term Primary Culture of Acinar-Intercalated Duct Complexes From Rat Submandibular Glands ,
In Vitro Cell Dev Biol
 22 , 469 , 1986
Cells
Acinar, submandibular gland
Enzymes
Hyaluronidase : 0.1 %
Medium
HBSS, CF
Pancreas
Rat
Rat, Dark Agouti and Lewis rat donors, adult
Sutton, R. , Peters, M. , McShane, P. , Gray, D. and Morris, P.
An Improved Method for the Isolation of Islets of Langerhans From the Adult Rat Pancreas ,
Transplant Proc
 XVII (6) , 1819 , 1986
Cells
Islets
Enzymes
Medium
Hank's solution
Pancreas
Rat
Rat, SD, male, 50-75 g
Brannon, P. , Orrison, B. and Kretchmer, N.
Primary Cultures of Rat Pancreatic Acinar Cells in Serum-Free Medium ,
In Vitro Cell Dev Biol
 21 (1) , 6 , 1985
Cells
Acinar
Enzymes
Hyaluronidase : 0.1%
Medium
HBSS
(see reference)
Pancreas
Rat
Rat, SD, 125 - 350 g
Githens, S. , Holmquist, D. , Whelan, J. and Ruby, J.
Ducts of the Rat Pancreas in Agarose Matrix Culture ,
In Vitro
 16 , 797 , 1980
Cells
Duct
Enzymes
Trypsin : 0.01%
Medium
HBSS
Pancreas
Rat
Rat
Schulz, I. , Heil, K. , Kribben, A. , Sachs, G. and Haase, W.
Isolation and Functional Characterization of Cells From the Exocrine Pancreas , Biology of Normal and Cancerous Exocrine Pancreatic Cells , Ribet, A., Pradayrol, L., and Susini, C. , Elsevier , 1980
Cells
Exocrine
Enzymes
Hyaluronidase : 0.9%
Medium
Kreb's Ringer
Pancreas
Rat
Rat, Wistar, male
Katada, T. and Ui, M.
Enhanced Insulin Secretion and Cyclic Amp Accumulation in Pancreatic Islets Due to Activation of Native Calcium Ionophores ,
J Biol Chem
 254 (2) , 469 , 1979
Cells
Islets
Enzymes
Medium
Medium 199
Pancreas
Rat
Rat, SD, male, 250 - 350 g
(also mouse, white, Swiss, male, 20 - 24 g)
Williams, J. , Korc, M. and Dormer, R.
Action of Secretagogues on a New Preparation of Functionally Intact, Isolated Pancreatic Acini ,
Am J Physiol
 235 , 517 , 1978
Cells
Acinar
Enzymes
Hyaluronidase : 0.18%
Medium
Kreb's Henseleit bicarbonate buffer
Pancreas
Rat
Rat, Wistar, male, 300-350 g
Wolters, G. , Konijenendijk, W. and Bouman, P.
Effects of Fasting on Insulin Secretion, Islet Glucose Metabolism, and the Cyclic Adenosine 3'5'-Monophosphate Content of Rat Pancreatic Islets In Vitro ,
Diabetes
 26 (6) , 530 , 1977
Cells
Islets
Enzymes
Collagenase : 0.2%
Medium
Kreb's Ringer bicarbonate buffer
Pancreas
Rat
Rat
Kondo, S. and Schulz, I.
Calcium Ion Uptake in Isolated Pancreas Cells Induced by Secretagogues ,
Biochim Biophys Acta
 419 , 76 , 1976
Cells
Exocrine
Enzymes
Hyaluronidase : 0.15%
Medium
Krebs
Pancreas
Rat
Rat, Wistar, male, 200 - 300 g
Shibata, A. , Ludvigsen, C. , Naber, S. , McKaneil M. and Lacy, P.
Standardization of a Digestion-Filtration Method for Isolation of Islets ,
Diabetes
 25 , 667 , 1976
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Rat
Rat, SD, albino, male, 150-200 g
Mangos, J. , McSherry, N. , Butcher, F. , Irwin, K. and Barber, T.
Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character) ,
Am J Physiol
 229 (3) , 560 , 1975
Cells
Acinar, parotid
Enzymes
Trypsin : 0.01%
Collagenase : 40-50 u/ml
Hyaluronidase : 0.10%
Medium
HBSS CMF
Pancreas
Rat
Rat, Wistar-Lewis, fetal 18-20 day gestation
(also neonatal 3-12 day old)
Braaten, J. , Jarlfors, U. , Smith, D. and Mintz, D.
Purification of Monolayer Cell Cultures of the Endocrine Pancreas ,
Tissue Cell
 7 (4) , 747 , 1975
Cells
Islets
Enzymes
Medium
EBSS
(see reference)
Pancreas
Rat
Rat, neonate
Leonard, R. , Lazarow, A. and Hegre, O.
Pancreatic Islet Transplantation in the Rat ,
Diabetes
 22 , 413 , 1973
Cells
Islets
Enzymes
Trypsin : 0.05%
Medium
Puck's saline buffered w/ EDTA 0.02%
Pancreas
Rat
Rat, male, albino, 200-300 g
Lacy, P. , Walker, M. and Fink, J.
Perifusion of Isolated Rat Islets in Vitro ,
Diabetes
 21 (10) , 987 , 1972
Cells
Islets
Enzymes
Collagenase : 0.5%
Medium
Hanks solution
Pancreas
Rat
Rat, Lewis, inbred
Ballinger, W. and Lacy, P.
Transplantation of Intact Pancreatic Islets in Rats ,
Surgery
 72 (2) , 175 , 1972
Cells
Islets
Enzymes
Collagenase : 0.5%
Medium
HBSS
Pancreas
Rat
Rat, Wistar, albino, male, 400 - 500 g
Lacy, P. and Kostianovsky, M.
Method for the Isolation of Intact Islets of Langerhans from the Rat Pancreas ,
Diabetes
 16 , 35 , 1967
Cells
Islets
Enzymes
Collagenase Type 4 : 1.0% - 1.2%
Medium
HBSS
Pancreas
Rat
Rat
Essner, E.
Experiments on an Ascites Hepatoma. I. Enzymatic Digestion and Alkaline Degradation of the Cementing Substance and Separation of Cells, in Tumor Islands ,
Exp Cell Res
 7 , 430 , 1954
Cells
Ascites hepatoma
Enzymes
Trypsin : 0.1%
Medium
Phosphate buffer (see reference)
Skin
Canine
Canine
Zheng, Y. , Nace, A. , Chen, W. , Watkins, K. , Sergott, L. , Homan, Y. , Vandeberg, J. , Breen, M. and Stenn, K.
Mature Hair Follicles Generated from Dissociated Cells: A Universal Mechanism of Folliculoneogenesis. ,
Dev Dyn
 239 , 2619-26 , 2010
Cells
Epidermal
Enzymes
Collagenase : 0.35%
Medium
DMEM
Skin
Frog
Frog
Nishikawa, A. , Shimizu-Nishikawa, K. and Miler, L.
Isolation, characterization, and in vitro culture of larval and adult epidermal cells of the frog Xenopus laevis ,
In Vitro Cell Dev Biol
 26 , 1128 , 1990
Cells
Epidermal
Enzymes
Trypsin : 0.18%
Medium
Barth's solution, CMF
Skin
Goat
Goat
Deng, Y. , Hu, J. and Athanasiou, K.
Isolation and Chondroinduction of a Dermis-Isolated, Aggrecan-Sensitive Subpopulation with High Chondrogenic Potential. ,
Arthritis Rheum
 56 , 168 , 2007
Cells
Dermis isolated aggrecan sensitive
Enzymes
Medium
DMEM
Skin
Human

Human

Burja, B. , Paul, D. , Tastanova, A. , Edalat, S. , Gerber, R. , Houtman, M. , Elhai, M. , Bürki, K. , Staeger, R. , Restivo, G. , Lang, R. , Sodin-Semrl, S. , Lakota, K. , Tomšič, M. , Levesque, M. , Distler, O. , Rotar, �. , Robinson, M. and Frank-Bertoncelj, M.
An Optimized Tissue Dissociation Protocol for Single-Cell RNA Sequencing Analysis of Fresh and Cultured Human Skin Biopsies. , Front Cell Dev Biol Vol. 10 , 872688 , 2022
Cells
Skin
Enzymes
Neutral Protease : 2.4 u/ml
Collagenase Type 4 : 1,000 u/ml
Medium
RPMI
Skin
Human
Human
Tan, J. , Jaafar, F. and Makpol, S.
Proteomic Profiling of Senescent Human Diploid Fibroblasts Treated with gamma-tocotrienol. ,
BMC Complement Altern Med
 18 , 314 , 2018
Cells
Diploid fibroblasts
Enzymes
Medium
DMEM
Skin
Human
Human
Botting, Ra. , Bertram, K. , Baharlou, H. , Sandgren, K. , Fletcher, J. , Rhodes, J. , Rana, H. , Plasto, T. , Wang, X. , Lim, J. , Barnouti, L. , Kohout, M. , Papadopoulos, T. , Merten, S. , Olbourne, N. and Cunningham, A.
Phenotypic and Functional Consequences of Different Isolation Protocols on Skin Mononuclear Phagocytes. ,
J Leukoc Biol
 101 , 1393-1403 , 2017
Cells
Mononuclear phagocytes
Enzymes
Neutral Protease : 1 u/ml
Collagenase : 200 u/ml
Trypsin : 0.5%
Medium
RPMI-1640
Skin
Human
Human
Chen, Z. , Wang, X. , Jin, T. , Wang, Y. , Hong, C. , Tan, L. , Dai, T. , Wu, L. , Zhuang, Z. and Shi, C.
Increase in the Radioresistance of Normal Skin Fibroblasts but not Tumor Cells by Mechanical Injury. ,
Cell Death Dis
 8 , e2573 , 2017
Cells
Fibroblasts
Enzymes
Medium
DMEM
Skin
Human
Human
Douvaras, P. , Wang, J. , Zimmer, M. , Hanchuk, S. , O'Bara, M. , Sadiq, S. , Sim, F. , Goldman, J. and Fossati, V.
Efficient Generation of Myelinating Oligodendrocytes from Primary Progressive Multiple Sclerosis Patients by Induced Pluripotent Stem Cells. ,
Stem Cell Reports
 3 , 250 , 2014
Cells
Skin fibroblasts
Enzymes
Collagenase Type 1 : 1000 u/ml
Medium
DMEM
Skin
Human
Human
Cheuk, S. , Wiken, M. , Blomqvist, L. , Nylen, S. , Talme, T. and Stahle, M.and Eidsmo, L.
Epidermal Th22 and Tc17 Cells Form a Localized Disease Memory in Clinically Healed Psoriasis. ,
J Immunol
 192 , 3111 , 2014
Cells
Skin
Enzymes
Medium
RPMI 1640
Skin
Human
Human
Cerny, D. , Haniffa, M. , Shin, A. , Bigliardi, P. , Tan, B. , Lee, B. , Poidinger, M. , Tan, E. , Ginhoux, F. and Fink, K.
Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection. ,
PLoS Pathog
 10 , e1004548 , 2014
Cells
Skin
Enzymes
Medium
RPMI

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.